Mangrove （Sonneratia spp.） could be found at Waai seashore, Ambon island. The remainder of the mangrove stem will be decayed and become the waste product. Some indigenous bacteria species that live in the decayed ma...Mangrove （Sonneratia spp.） could be found at Waai seashore, Ambon island. The remainder of the mangrove stem will be decayed and become the waste product. Some indigenous bacteria species that live in the decayed mangrove stem waste product have cellulolytic and amylolytic characters. This research was done to： （1） identify and determine the cellulolytic bacteria species; （2） identify and determine the amylolytic bacteria species; （3） determine the cellulolytic bacteria species that have the highest cellulase activity; （4） determine the amylolytic bacteria species that have the highest amylase activity. This research was conducted at the Microbiology Laboratory, Department of Biology, State University of Malang, Microbiology Laboratory, Faculty of Medicine, Brawijaya University and Chemistry Laboratory, Muhammadiyah Malang University. In the experiment, 25 g sample were grinded and diluted in 225 mL nutrient broth to get 101 suspension. Then the suspension was diluted gradually until 101~. The suspension was inoculated on nutrient agar medium with 0.1 mL each, and incubated in 37 ~C during 24 h. Each bacteria colony was isolated and identified to know whether it was cellulolytic or amylolytic bacteria. Afterward, the cellulase activity as well as the amylase activity was analyzed. The research results show that： （1） there are four cellulolytic bacteria species, i.e., Micrococcus luteus, Bacillus pumilus, Planococcus citreus and Bacillus cereus; （2） there are four amylolytic bacteria species, i.e., Bacillus firmus, Nitrobacter sp., Bacillus mycoides and Pseudomonas aeruginosa; （3） Bacillus cereus has the highest cellulase activity; （4） Nitrobacter sp. has the highest amylase activity.展开更多
Oriental Beech is the most important commercial tree species in northern Iran. In recent years wood production companies interested in felling large beech trees for profit have challenged advocates of close-to-nature ...Oriental Beech is the most important commercial tree species in northern Iran. In recent years wood production companies interested in felling large beech trees for profit have challenged advocates of close-to-nature silviculture who favor conservation. Our study objective was to assess the economic value of over-mature beech trees by relating tree diameter （DBH） to amount of decay. Based on the location of onset of decay, we categorized three types of decay as stump, stem, and crown decay. Trees of greater diameter （age） typically showed greater decay in the stem. Percent of decayed volume, diameter of decayed tissue, and length of decay in tree stems varied between 0.5%-64.3%, 15 cm-75 cm, and 2.0-19.5 m, respectively. With increasing trunk diameter, the propor- tion of truck decay increased. Red heart and dark red heart constituted 25% and 14.3% of sampled trees, respectively. However, we found no correlation between intensity of stem decay and morphological charac- teristics of trees. Seedlings were not abundant around the bases of over-mature trees, suggesting that the trees did not contribute to regeneration of the stand. Beech trees of diameter 〉1 m do not provide valu- able round wood for industries and cause to raise wood production costs. We recommend that these trees 〉1 m DBH should be retained in forest stands because of their low commercial value but high ecological and conservational values such as maintaining biodiversity in forest ecosystems.展开更多
文摘Mangrove （Sonneratia spp.） could be found at Waai seashore, Ambon island. The remainder of the mangrove stem will be decayed and become the waste product. Some indigenous bacteria species that live in the decayed mangrove stem waste product have cellulolytic and amylolytic characters. This research was done to： （1） identify and determine the cellulolytic bacteria species; （2） identify and determine the amylolytic bacteria species; （3） determine the cellulolytic bacteria species that have the highest cellulase activity; （4） determine the amylolytic bacteria species that have the highest amylase activity. This research was conducted at the Microbiology Laboratory, Department of Biology, State University of Malang, Microbiology Laboratory, Faculty of Medicine, Brawijaya University and Chemistry Laboratory, Muhammadiyah Malang University. In the experiment, 25 g sample were grinded and diluted in 225 mL nutrient broth to get 101 suspension. Then the suspension was diluted gradually until 101~. The suspension was inoculated on nutrient agar medium with 0.1 mL each, and incubated in 37 ~C during 24 h. Each bacteria colony was isolated and identified to know whether it was cellulolytic or amylolytic bacteria. Afterward, the cellulase activity as well as the amylase activity was analyzed. The research results show that： （1） there are four cellulolytic bacteria species, i.e., Micrococcus luteus, Bacillus pumilus, Planococcus citreus and Bacillus cereus; （2） there are four amylolytic bacteria species, i.e., Bacillus firmus, Nitrobacter sp., Bacillus mycoides and Pseudomonas aeruginosa; （3） Bacillus cereus has the highest cellulase activity; （4） Nitrobacter sp. has the highest amylase activity.
文摘Oriental Beech is the most important commercial tree species in northern Iran. In recent years wood production companies interested in felling large beech trees for profit have challenged advocates of close-to-nature silviculture who favor conservation. Our study objective was to assess the economic value of over-mature beech trees by relating tree diameter （DBH） to amount of decay. Based on the location of onset of decay, we categorized three types of decay as stump, stem, and crown decay. Trees of greater diameter （age） typically showed greater decay in the stem. Percent of decayed volume, diameter of decayed tissue, and length of decay in tree stems varied between 0.5%-64.3%, 15 cm-75 cm, and 2.0-19.5 m, respectively. With increasing trunk diameter, the propor- tion of truck decay increased. Red heart and dark red heart constituted 25% and 14.3% of sampled trees, respectively. However, we found no correlation between intensity of stem decay and morphological charac- teristics of trees. Seedlings were not abundant around the bases of over-mature trees, suggesting that the trees did not contribute to regeneration of the stand. Beech trees of diameter 〉1 m do not provide valu- able round wood for industries and cause to raise wood production costs. We recommend that these trees 〉1 m DBH should be retained in forest stands because of their low commercial value but high ecological and conservational values such as maintaining biodiversity in forest ecosystems.
