Chemical Compositions of Stemmacantha carthamoides Root

[Objectives] Chemical compositions of S. carthamoides root were isolated and identified. [Methods]Solvent extraction,repeated silica gel column chromatography,ODS column chromatography,preparative liquid chromatography and recrystallization were used for separation and purification,and chemical structure of compound was identified via its physical-chemical properties and spectral analysis data.[Results] Eight compounds were obtained from methanol extract of S. carthamoides root: 24-ethyl-20-oxhydryl ecdysone( 1),β-ecdysterone( 2),Ajugasterone C( 3),3,4-DHBA( 4),3-methoxy-quercetin( 5),( 7 S,8 S)-syringoylglycerol( 6),( E)-3,7-dimethyl-1-O-[α-L-arabinofuranosyl-( 1→6)-β-D-glucopyranosy1]-oct-2-en-7-ol( 7),Polypodine B( 8). [Conclusions] Compounds 6 and 7 were firstly isolated from the plant.

漏芦中蜕皮甾酮提取方法研究

目的:优选漏芦中蜕皮甾酮的最佳提取工艺条件。方法:以蜕皮甾酮含量及固体物含量为指标,比较三种不同提取方法:不同溶剂回流法,超声提取法,冷浸法。结果:三种溶剂以甲醇提取蜕皮甾酮最完全,水其次,乙醇受浓度影响很大;超声提取所需时间短,提取基本完全;冷浸法所需时间长,提取不完全。结论:甲醇热提取法效果最好。

麻花头属和漏芦属药用植物中蜕皮甾酮类成分及其生物活性研究

漏芦为传统中药之一。本文概述我国麻花头属药用植物(华麻花头、蕴苞麻花头、麻花头、伪泥胡菜)和漏芦属药用植物(漏芦和鹿草)所含蜕皮甾酮类化合物的研究,分析其化学结构特点,为确定这些中药的药效物质基础和开发现代中药提供科学依据。上述药用植物所含蜕皮甾酮类化合物具有化学结构多样性的特点,与其生物学活性具有密切相关性。

鹿草根中β-蜕皮甾酮的含量测定

目的:建立哈萨克药鹿草根中β-蜕皮甾酮的含量测定方法。方法:采用反相,使用Waters XTerra RP C18(250 mm×4.6 mm,5μm)色谱柱,以甲醇-1%醋酸溶液(27∶73)为流动相,体积流量为1.0 mL.min-1,检测波长为250 nm,柱温30℃。结果:在上述条件下,β-蜕皮甾酮浓度在1.45～14.5μg.mL-1范围内呈良好的线性关系(r=0.9998);平均加样回收率(n=9)为100.4%。结论:本方法准确,灵敏度高,重现性好,能有效测定鹿草根中β-蜕皮甾酮含量。