Report of a Dicephalic Steppes Ratsnake (Elaphe dione) Collected in South Korea

In this report, we describe morphological characteristics of a dicephalic Steppes Ratsnake （Elaphe dione） collected from the wild in 2011 in South Korea. The specimen has two heads and two long necks. Unlike normal individuals, the dicephalic snake has divided ventral scales under the necks of the bifurcated columns. The snout- vent length （SVL） and overall total length of the individual are shorter than those of normal snakes of the same age. Nevertheless, the counts of nine different scale types that are often used for classification are all within the ranges of normal individuals. As far as we know, this is the first detailed morphological description of a dicephalic E. dione in the scientific literature.

The Impact of Current Climate Changes on Morphodynamic Regimes of Steppes and Forest Steppes in Southern Siberia

Predictive assessments of the geomorphological systems’ behavior under climate warming in southern Siberia have been performed based on time-series data, using theoretical and empirical models of processes. High sensitivity and fast response of erosional, aeolian, and cryogenic processes to current climatic changes are presented. The current trends under changing functioning conditions of geomorphological systems while maintaining climatic trends have been determined. Areas with different targeting of morpholithogenesis are identified.

Comparison of CO_(2) Effluxes and Their Driving Factors Between Two Temperate Steppes in Inner Mongolia, China

Soil respiration is a key component of the global terrestrial ecosystem carbon cycle. The static opaque chamber method was used to measure the CO2 effiuxes from soil of a semiarid Aneurolepidium chinense steppe and a Stipa krylovii steppe in the Xilin River Basin of Inner Mongolia, China from March 2002 to December 2004. The results indicated that the soil respiration rates of the semiarid Aneurolepidium chinense steppe and the Stipa krylovii steppe were both relatively high from mid-May to mid-September of each year and remained low during the rest of the year. The minimum value of soil respiration occurred in December or January and negative effiuxes of CO2 appeared for several days during the non-growing season of individual years at the two sampling sites. A high annual variation was found in the two steppes with the coefficients of variance （CV） being over 94%, even high to 131%. The annual sums of soil CO2 effiux of the Aneurolepidium chinense steppe varied between 356.4 gC m^-2 yr^-1 and 408.8 gC m^-2 yr^-1, while those of the Stipa krylovii steppe in the three years were in the range of 110.6 gC m^-2 yr^-1 to 148.6 g Cm^-2 yr^-1. The mean respiration rates of the Aneurolepidium chinense steppe were significantly higher than those of the Stipa krylovii steppe in different statistical periods with the exception of the non-growing season. About 59.9% and 80.6% of the soil respiration variations in both steppes for the whole sampling period were caused by the changes of temperature and soil water content. In the Aneurolepidium chinense steppe, the soil respiration rate has significant or extremely significant positive correlation （r = 0.58 - 0.85, p 〈 0.05 or p 〈 0.01） with air temperature and ground temperature of the topsoil except in 2002; the unique contributions of temperature change to the soil respiration variation of the three years were 53.3%, 81.0% and 58.6%, respectively. But, for the Stipa krylovii steppe in the same time interval, the soil water content （especially that of the 10-20 cm layer） has a greater effect on the change of soil respiration, and the unique contributions of the change of the 10-20 cm soil water content to the variations of soil respiration in 2002 and 2003 were 60.0% and 54.3%, respectively. In 2004, in spite of the higher contribution of temperature than soil water content, the contribution of ground temperature at a depth of 10 cm was only 46.2%, much weaker than that of any single year in the Aneurolepidium chinense steppe.

Evaluation of Plant Diversity in the Steppes of White Wormwood of the Region of Saida (Western Algeria)

This work is an evaluation of the plant diversity of the white Wormwood formations (Artemisia herba-alba Asso.) in the steppes rangelands of the south-eastern zone of Sa?da (municipality of Maamora, Western Algeria). For this purpose, twenty-one phytoecological statements are carried out on three sample stations: an exclosure station where the white Wormwood is well developed and two other stations not protected where in one, the white Wormwood is moderately degraded and in the other one, it is strongly degraded. The factorial correspondences analysis (FCA) and the ascending hierarchical clustering (AHC) carried out on the floristic and edaphic parameters measured on these stations led to a hierarchy of the ecological factors determining the diversity of the studied plant formations with white Wormwood. The vegetation studied is marked by the dominance of the therophytes, particularly in the third station (very degraded station), while the exclosure station is essentially characterized by a high recovery rate and an important phytomass.

Evaluation of Characteristics Floristico-Edaphic of the Steppes at Alfa (<i>Stipa tenacissima</i>L.) in the Saida Region (Western Algeria)

To establish an assessment of floristic and edaphic characteristics of steppe formations we conducted a phytoecological study on a local scale by comparing the current plant diversity and soil in three (3) stations representative of the vegetation to alfa (Stipa tenacissima L.) located south of Saida (western Algeria). The relations between the station, the vegetation and the characteristics of soils in the steppe are very imperfectly known. This study intends to analyze the relationship between floristic composition and edaphic parameters of the steppe at alfa. Anthropogenic pressures on plant structure are different imbalance resulting in one ecological. The floristic and edaphic data have been the subject of a factorial correspondence analysis (A.F.C). The floristic inventory denotes a regressive dynamics of the formations at alfa passing from one site to another. Thus 39 plant species have been inventoried in the site in good condition, against 26 species for the moderately degraded site and 16 species only for the damaged site. The coupled results between species and edaphic parameters show the relations between the plant diversity and the physico-chemical characteristics of the soils. The statistical analyses do appear a strong correlation between floristic composition, the state of vegetation and the edaphic parameters.

基于TeamSTEPPS的出院准备联动干预在老年缺血性卒中患者中的应用

目的探讨基于Team STEPPS的出院准备联动干预在老年缺血性卒中患者中的应用。方法选取2022年10月—2023年9月在安徽医科大学附属巢湖医院神经内科住院的106例老年缺血性卒中患者为研究对象,按入院时间将2022年10月—2023年3月入院的51例患者作为对照组,予以常规出院护理及随访;2023年4月—2023年9月入院的55例患者为干预组,实施基于Team STEPPS的出院准备联动干预。比较两组患者的出院准备度、主要照顾者过渡期的准备情况以及出院90 d患者日常生活活动能力、不良事件发生及非计划再入院情况。结果干预组患者出院准备度评估量表总分及4个维度得分、主要照顾者过渡期准备情况评估量表总分及8个维度得分均高于对照组,差异有统计学意义(P均<0.01)。干预组患者出院90 d日常生活活动能力评分为55(40,65)分,高于对照组的40(20,55)分,差异有统计学意义(P<0.01)。干预组患者出院90 d发生跌倒/坠床2例、压力性损伤3例、非计划性拔管4例,均低于对照组的8、10、11例,差异有统计学意义(P均<0.05)。干预组患者出院90 d非计划再入院2例,少于对照组的8例,差异有统计学意义(P=0.046)。结论基于Team STEPPS的出院准备联动干预可提升老年缺血性卒中患者出院准备度,改善主要照顾者过渡期的准备情况,提高患者出院90 d后日常生活活动能力,降低不良事件发生及非计划再入院,为老年缺血性卒中患者出院准备提供一定的临床依据。

Responses of plant diversity and soil microorganism diversity to nitrogen addition in the desert steppe,China

Nitrogen(N)deposition is a significant aspect of global change and poses a threat to terrestrial biodiversity.The impact of plant-soil microbe relationships to N deposition has recently attracted considerable attention.Soil microorganisms have been proven to provide nutrients for specific plant growth,especially in nutrient-poor desert steppe ecosystems.However,the effects of N deposition on plant-soil microbial community interactions in such ecosystems remain poorly understood.To investigate these effects,we conducted a 6-year N-addition field experiment in a Stipa breviflora Griseb.desert steppe in Inner Mongolia Autonomous Region,China.Four N treatment levels(N0,N30,N50,and N100,corresponding to 0,30,50,and 100 kg N/(hm2•a),respectively)were applied to simulate atmospheric N deposition.The results showed that N deposition did not significantly affect the aboveground biomass of desert steppe plants.N deposition did not significantly reduce the alfa-diversity of plant and microbial communities in the desert steppe,and low and mediate N additions(N30 and N50)had a promoting effect on them.The variation pattern of plant Shannon index was consistent with that of the soil bacterial Chao1 index.N deposition significantly affected the beta-diversity of plants and soil bacteria,but did not significantly affect fungal communities.In conclusion,N deposition led to co-evolution between desert steppe plants and soil bacterial communities,while fungal communities exhibited strong stability and did not undergo significant changes.These findings help clarify atmospheric N deposition effects on the ecological health and function of the desert steppe.

Effects of long-term fencing on soil microbial community structure and function in the desert steppe,China

One of the goals of grazing management in the desert steppe is to improve its ecosystem.However,relatively little is known about soil microbe communities in the desert steppe ecosystem under grazing management.In this study,we investigated the diversity and aboveground biomass of Caragana korshinskii Kom.shrub communities in long-term fencing and grazing areas,combined with an analysis of soil physical-chemical properties and genomics,with the aim of understanding how fence management affects plant-soil-microbial inter-relationships in the desert steppe,China.The results showed that fence management(exclosure)increased plant diversity and aboveground biomass in C.korshinskii shrub area and effectively enhanced soil organic carbon(233.94%),available nitrogen(87.77%),and available phosphorus(53.67%)contents.As well,the Shannon indices of soil bacteria and fungi were greater in the fenced plot.Plant-soil changes profoundly affected the alpha-and beta-diversity of soil bacteria.Fence management also altered the soil microbial community structure,significantly increasing the relative abundances of Acidobacteriota(5.31%-8.99%),Chloroflexi(3.99%-5.58%),and Glomeromycota(1.37%-3.28%).The soil bacterial-fungal co-occurrence networks under fence management had higher complexity and connectivity.Based on functional predictions,fence management significantly increased the relative abundance of bacteria with nitrification and nitrate reduction functions and decreased the relative abundance of bacteria with nitrate and nitrite respiration functions.The relative abundances of ecologically functional fungi with arbuscular mycorrhizal fungi,ectomycorrhizal fungi,and saprotrophs also significantly increased under fence management.In addition,the differential functional groups of bacteria and fungi were closely related to plant-soil changes.The results of this study have significant positive implications for the ecological restoration and reconstruction of dry desert steppe and similar areas.

Spatial-temporal variation in soil respiration and its controlling factors in three steppes of Stipa L. in Inner Mongolia, China

Grassland is the largest terrestrial ecosystem in China. It is of great significance to measure accurately the soil respiration of different grassland types for the contribution evaluation of the Chinese terrestrial ecosystem’s carbon emission to the atmospheric CO2 concentration. A three-year (2005-2007) field experiment was carried out on three steppes of Stipa L. in the Xilin River Basin, Inner Mongolia, China, using a static opaque chamber technique. The seasonal and interannual variations of soil respiration rates were analyzed, and the annual total soil respiration of the three steppes was estimated. The numerical models between soil respiration and water-heat factors were established respectively. Similar seasonal dynamic and high annual and interannual variations of soil respiration were found in all of the three steppes. In the growing season, the fluctuation of soil respiration was particularly evident. The coefficients of variation (CVs) for soil respiration in different growing seasons ranged from 54% to 93%, and the annual CVs were all above 115%. The interannual CV of soil respiration progressively decreased in the order of Stipa grandis (S. grandis) steppe > Stipa baicalensis (S. baicalensis) steppe > Stipa krylovii (S. krylovii) steppe. The annual total soil respiration for the S. baicalensis steppe was 223.62?299.24 gC m-2 a-1, 150.62-226.99 gC m-2 a-1 for the S. grandis steppe, and 111.31–131.55 gC m-2 a-1 for the S. krylovii steppe, which were consistent with the precipitation gradient. The variation in the best fitting temperature factor explained the 63.5%, 73.0%, and 73.2% change in soil respiration in the three steppes at an annual time scale, and the corresponding Q10 values were 2.16, 2.98, and 2.40, respectively. Moreover, the Q10 values that were calculated by soil temperature at different depths all expressed a 10 cm > 5 cm > surface in the three sampling sites. In the growing season, the soil respiration rates were related mostly to the surface soil moisture, and the 95.2%, 97.4%, and 93.2% variations in soil respiration in the three steppes were explained by the change in soil moisture at a depth of 0-10 cm, respectively.

Stipa steppes in scantily explored regions of the tibetan Plateau:classification,community characteristics and climatic distribution patterns

Aims As a unique geographical unit of the earth,the tibetan Plateau is extensively covered by various Stipa communities.However,their vegetation features have not been reported systematically till now,especially in some scantily explored regions.In this study,we endeavor to reveal the community types,quantitative charac-teristics and climatic distribution patterns of Stipa steppes in these areas based on primary relevés obtained from fieldwork.Methods We collected a total of 223 plots in 79 study sites in the Changthang Plateau and the Yarlung Zangbo Valley,ranging from 79°E to 91°E.the categories of Stipa formations were identified according to the classification scheme in Vegetation of China and then verified by Nonmetric Multidimensional Scaling.We performed detrended correspondence analysis and detrended canonical correspondence analysis to hunt for the alteration of Stipa communities along the precipitation gradient.Quantitative characteristics including species richness,coverage,biomass as well as importance values(IV)of dominant species were calcu-lated and visualized,respectively.Important Findings Stipa steppes in scantily explored regions of the tibetan Plateau are classified into 11 formations but major formations are rather limited in number.Formation(form.)Stipa purpurea is the most widespread Stipa assemblages not only in scantily explored regions but also across the whole tibetan Plateau.the characteristics of Stipa com-munities,including coverage,species richness,productivity and IV of dominant species,demonstrate the features of typical alpine steppes on the tibetan Plateau.Precipitation proves to be the prime climatic factor controlling the distribution patterns of Stipa assemblages.Form.Stipa subsessiliflora var.basiplumosa and form.Stipa glareosa normally distribute in arid habitats,but rainfall for the former is of greater variance.Form.Stipa roborowskyi and form.Stipa capillacea favor moderately moist environment.Form.Stipa purpurea and form.Stipa roborowskyi can tolerate a fairly broad range of precipitation.

Soil respiration associated with plant succession at the meadow steppes in Songnen Plain, Northeast China

Aims Soil CO_(2) emission from steppes is affected by soil properties and vegetation in different successional stages.Primary and secondary succession of plants frequently occurred at the meadow steppe in Songnen Plain,Northeast China,which indicates the large uncer-tainty associated with CO_(2) emission in this environment.This study aims to investigate the temporal variations of soil respiration(Rs)and the effect of plant succession on cumulative soil CO_(2) emission during the growing season.Methods Using a LI-6400 soil CO_(2) flux system,Rs of five vegetation types which represented different stages of plant succession in meadow steppes of Songnen Plain,China,was investigated during the grow-ing seasons of 2011 and 2012.Important Findings Soil temperature(Ts)was the dominant controlling factor of Rs,which could explain~64%of the change in CO_(2) fluxes.The Q10 values of Rs were ranged from 2.0 to 6.7,showing a decreasing trend with the plant successional stages.The cumulative CO_(2) emis-sion increased with the degree of vegetation succession and it aver-aged to 316±6 g C m^(−2)(ranges:74.8±6.7 to 516.5±11.4 g C m^(−2))during the growing season.The magnitude of soil CO_(2) emission during the growing season was positively correlated with above-ground plant biomass,soil organic carbon content and mean soil water content,while negatively linked to mean Ts,pH,electrical conductivity and exchangeable sodium percentages.The results implied that soil CO_(2) emission increased with the development of plant communities toward more advanced stages.Our findings pro-vided valuable information for understanding the variations of CO_(2) emission in the process of vegetation succession.

Trends in extreme climatic indices across the temperate steppes of China from 1961 to 2013

Aims Extreme climate events have become more severe and frequent with global change in recent years.The Chinese temperate steppes are an important component of the Eurasian steppes and highly sensitive and vulnerable to climatic change.As a result,the occur-rence of extreme climate events must have strong impacts on the temperate steppes.Therefore,understanding the spatio-temporal trends in extreme climate is important for us to assess the sensitivity and vulnerability of Chinese temperate steppes to climatic changes.This research had two specific objects to(i)specify the temporal changes in extreme climate events across the whole steppe and(ii)compare the trend differences for extreme climate events in differ-ent types of steppes-meadow steppe,typical steppe and desert steppe.Methods To investigate extreme climate trends in the temperate steppes of China,82 meteorological stations with daily temperature and precipi-tation data(1961-2013)were used.Meanwhile,eight core extreme climate indices(extreme high-temperature threshold,extreme low-temperature threshold,frost days,heatwave duration,heavy rain-fall threshold,percentage of heavy rainfall,heavy rainfall days and consecutive dry days)from the Statistical and Regional Dynamical Downscaling of Extremes for European Regions(STARDEX)project were selected to analyse the trends in extreme climate across the whole temperate steppe and the three main types(meadow steppe,typical steppe and desert steppe)through time and space.Important Findings The results showed that(i)the changes in extreme climatic tem-perature events across the whole temperate steppe were obvi-ous during 1961-2013.The frost days(−3.40 days/10 year[yr])decreased significantly,while the extreme high-temperature thresh-old(0.24℃/10 yr),extreme low-temperature threshold(0.52℃/10 yr),and heatwave duration(0.58 days/10 yr)increased notably.The annual changes in extreme precipitation were small and not sig-nificant.(ii)Differences appeared in the extreme climatic trends in different types of steppes.The desert steppe showed strong climate extremes and underwent the most significant asymmetric warming compared with the meadow steppe and typical steppe.At the same time,the heatwave duration(0.62 days/10 yr)increased.In terms of the extreme precipitation,there was no significant trend among the three types of steppes.However,the fluctuations in extreme precipi-tation were the largest in the desert steppe compared to those in the typical steppe and meadow steppe.

基于Team STEPPS模型强化呼吸道管理在机械通气病人中的应用

目的:探讨基于Team STEPPS模型强化呼吸道管理在机械通气病人中的应用。方法:回顾性分析2022年1月—11月在医院重症监护室(ICU)接受机械通气的124例病人的临床资料,根据气道管理方式不同分为对照组(50例)和研究组(74例)。对照组病人接受常规护理干预,研究组病人接受基于Team STEPPS模型强化呼吸道管理。比较两组机械通气时间;ICU入住时间;入住ICU第1天和第7天的动脉血氧分压(PaO_(2))、血氧饱和度(SaO_(2))及氧合指数变化;并记录两组住院期间并发症发生情况。结果:研究组机械通气时间和ICU入住时间均短于对照组(P<0.05);两组入住ICU第7天PaO_(2)、SaO_(2)及氧合指数高于第1天,且研究组均高于对照组(P<0.05);研究组病人住院期间并发症总发生率低于对照组(P<0.05)。结论:基于Team STEPPS模型的强化呼吸道管理可缩短机械通气病人机械通气时间和ICU入住时间,改善病人血气指标,纠正低氧血症状态,降低并发症发生率,改善病人预后。

Biocrust-induced partitioning of soil water between grass and shrub in a desert steppe of Northwest China

Maintaining the stability of exotic sand-binding shrub has become a large challenge in arid and semi-arid grassland ecosystems in northern China.We investigated two kinds of shrublands with different BSCs(biological soil crusts)cover in desert steppe in Northwest China to characterize the water sources of shrub(Caragana intermedia Kuang et H.C.Fu)and grass(Artemisia scoparia Waldst.et Kit.)by stable 18O isotopic.Our results showed that both shrublands were subject to persistent soil water deficiency from 2012 to 2017,the minimum soil depth with CV(coefficient of variation)<15% and SWC(soil water content)<6% was 1.4 m in shrubland with open areas lacking obvious BSC cover,and 0.8 m in shrubland covered by mature BSCs.For C.intermedia,a considerable proportion of water sources pointed to the surface soil.Water from BSCs contributed to averages 22.9%and 17.6%of the total for C.intermedia and A.scoparia,respectively.C.intermedia might use more water from BSCs in rainy season than dry season,in contrast to A.scoparia.The relationship between shrub(or grass)and soil water by δ^(18)O shown significant differences in months,which partly verified the potential trends and relations covered by the high variability of the water source at seasonal scale.More fine roots at 0-5 cm soil layer could be found in the surface soil layer covered by BSCs(8000 cm/m^(3))than without BSCs(3200 cm/m^(3)),which ensured the possibility of using the surface soil water by C.intermedia.The result implies that even under serious soil water deficiency,C.intermedia can use the surface soil water,leading to the coexistence between C.intermedia and A.scoparia.Different with the result from BSCs in desert areas,the natural withdrawal of artificial C.intermedia from desert steppe will be a long-term process,and the highly competitive relationship between shrubs and grasses also determines that its habitat will be maintained in serious drought state for a long time.

Response of soil respiration to short-term changes in precipitation and nitrogen addition in a desert steppe

Changes in precipitation and nitrogen(N)addition may significantly affect the processes of soil carbon(C)cycle in terrestrial ecosystems,such as soil respiration.However,relatively few studies have investigated the effects of changes in precipitation and N addition on soil respiration in the upper soil layer in desert steppes.In this study,we conducted a control experiment that involved a field simulation from July 2020 to December 2021 in a desert steppe in Yanchi County,China.Specifically,we measured soil parameters including soil temperature,soil moisture,total nitrogen(TN),soil organic carbon(SOC),soil microbial biomass carbon(SMBC),soil microbial biomass nitrogen(SMBN),and contents of soil microorganisms including bacteria,fungi,actinomyces,and protozoa,and determined the components of soil respiration including soil respiration with litter(RS+L),soil respiration without litter(RS),and litter respiration(RL)under short-term changes in precipitation(control,increased precipitation by 30%,and decreased precipitation by 30%)and N addition(0.0 and 10.0 g/(m^(2)·a))treatments.Our results indicated that short-term changes in precipitation and N addition had substantial positive effects on the contents of TN,SOC,and SMBC,as well as the contents of soil actinomyces and protozoa.In addition,N addition significantly enhanced the rates of RS+L and RS by 4.8%and 8.0%(P<0.05),respectively.The increase in precipitation markedly increased the rates of RS+L and RS by 2.3%(P<0.05)and 5.7%(P<0.001),respectively.The decrease in precipitation significantly increased the rates of RS+L and RS by 12.9%(P<0.05)and 23.4%(P<0.001),respectively.In contrast,short-term changes in precipitation and N addition had no significant effects on RL rate(P>0.05).The mean RL/RS+L value observed under all treatments was 27.63%,which suggested that RL is an important component of soil respiration in the desert steppe ecosystems.The results also showed that short-term changes in precipitation and N addition had significant interactive effects on the rates of RS+L,RS,and RL(P<0.001).In addition,soil temperature was the most important abiotic factor that affected the rates of RS+L,RS,and RL.Results of the correlation analysis demonstrated that the rates of RS+L,RS,and RL were closely related to soil temperature,soil moisture,TN,SOC,and the contents of soil microorganisms,and the structural equation model revealed that SOC and SMBC are the key factors influencing the rates of RS+L,RS,and RL.This study provides further insights into the characteristics of soil C emissions in desert steppe ecosystems in the context of climate change,which can be used as a reference for future related studies.

Responses of vegetation yield to precipitation and reference evapotranspiration in a desert steppe in Inner Mongolia,China

Drought,which restricts the sustainable development of agriculture,ecological health,and social economy,is affected by a variety of factors.It is widely accepted that a single variable cannot fully reflect the characteristics of drought events.Studying precipitation,reference evapotranspiration(ET_(0)),and vegetation yield can derive information to help conserve water resources in grassland ecosystems in arid and semi-arid regions.In this study,the interactions of precipitation,ET_(0),and vegetation yield in Darhan Muminggan Joint Banner(DMJB),a desert steppe in Inner Mongolia Autonomous Region,China were explored using two-dimensional(2D)and three-dimensional(3D)joint distribution models.Three types of Copula functions were applied to quantitatively analyze the joint distribution probability of different combinations of precipitation,ET_(0),and vegetation yield.For the precipitation–ET_(0)dry–wet type,the 2D joint distribution probability with precipitation≤245.69 mm/a or ET_(0)≥959.20 mm/a in DMJB was approximately 0.60,while the joint distribution probability with precipitation≤245.69 mm/a and ET_(0)≥959.20 mm/a was approximately 0.20.Correspondingly,the joint return period that at least one of the two events(precipitation was dry or ET_(0)was wet)occurred was 2 a,and the co-occurrence return period that both events(precipitation was dry and ET_(0)was wet)occurred was 5 a.Under this condition,the interval between dry and wet events would be short,the water supply and demand were unbalanced,and the water demand of vegetation would not be met.In addition,when precipitation remained stable and ET_(0)increased,the 3D joint distribution probability that vegetation yield would decrease due to water shortage in the precipitation–ET_(0)dry–wet years could reach up to 0.60–0.70.In future work,irrigation activities and water allocation criteria need to be implemented to increase vegetation yield and the safety of water resources in the desert steppe of Inner Mongolia.

MED4 gene positively affects preadipocyte differentiation in Chinese red steppe cattle

Background:The regulatory genes related to lipid metabolism affect the intramuscular fat(IMF)content and improve meat quality traits.Mediator Complex Subunit 4(MED4),a vitamin D receptor protein,affects vitamin D,livestock growth,carcass traits,and triglyceride deposition.However,the physiological function of the MED4 gene on bovine adipocyte differentiation remains unknown.Methods:This study explored the function of the MED4 gene in preadipocyte differentiation in Chinese Red Steppe cattle.The overexpression plasmid and the interference sequences of the MED4 gene were constructed to detect the effects of the MED4 gene on adipogenesis and biomakers using quantitative polymerase chain reaction and western blotting.Results:The MED4 gene had significantly high expression during preadipocyte differentiation(p<0.05).Overexpression of the MED4 gene increased the expression of the PPARγgene,a preadipocyte differentiation biomarker,improved cellular lipid droplets and triglycerides accumulation,and positively accelerated adipocyte maturation(p<0.05).Interference of the MED4 gene can negatively regulate preadipocyte differentiation.Conclusion:This study showed that the MED4 gene may affect the preadipocyte differentiation and adipogenesis in Chinese Red Steppe cattle by regulating the PPARγgene.

Team STEPPS模型在“ICU-病房”肺部延伸护理中的应用效果评价

目的:评价基于提高医疗质量和患者安全的团队策略和工具包(Team Strategies and Tools to Enhance Performance and Patient Safety,Team STEPPS)构建的"ICU-病房"肺部延伸护理模式的应用效果。方法:回顾性选取2016年1-12月符合纳入标准的术后患者为对照组(98例),给予常规护理。纳入2017年1-12月符合纳入标准的术后患者为实验组(135例),运用基于Team STEPPS模型构建的"ICU-病房"肺部延伸护理模式。结果:实验组患者非计划重返ICU率、因肺部原因重返ICU率均低于对照组,患者对护理工作满意度评分高于对照组,差异均有统计学意义(P<0.05)。结论:运用基于Team STEPPS模型构建的"ICU-病房"肺部延伸护理模式可降低患者重返ICU率,提高患者满意度。

草原红牛转铁蛋白和后转铁蛋白多态性及其与生产性能相关性研究(英文)

[Objective] The aim of this study was to investigate the correlation between blood protein polymorphism of red steppe and its performance.[Method]Two blood protein polymorphic loci were detected in transferring(Tf)and posttremsferr(Ptf)from thirteen red steppes and eighteen hybrid of limousin and red steppe by polyacrylamide gel electrophoresis.[Result]Tf and Ptf were controlled by three and two alleles respectively.[Conclusion]The variance analysis of blood protein polymorphic loci and its performance indicates that two protein loci have a positive or negative correlation with some traits of red steppe and the improved limousin cattle population.

内蒙古高原荒漠草原植物气候生产力时空演变特征(英文)

Thornthwaite Memorial model and other statistic methods were used to calculate the climate-productivity of plants with the meteorological data from 1961 to 2007 at 9 stations distributed on Inner Mongolia desert steppe.The spatial and temporal variation characteristics of climate-productivity were analyzed by using the methods of the tendency rate of the climate trend,accumulative anomaly,and spatial difference and so on.The results showed that the climate-productivity kept linear increased trend over Inner Mongolia desert steppe in recent 47 years,but not significant.In spatial distribution,the climate-productivity reduced with the increased latitude.The climate-productivity in southwest part of Inner Mongolia desert steppe was growing while that in the southeast was reducing.The variation rate of the climate-productivity increased from the northwest part to the southeast part of Inner Mongolia desert steppe.In recent 47 years,the climate-productivity in southeast Jurh underwent the greatest decreasing extent,and the region was the sensitive area of the climate-productivity variation.