The worldwide use of Stevia rebaudiane as substitute sweetener for sugar, increases every year. Stevia rebaudiane is not involved in the insulin mechanism and as so has no calories. This makes Stevia rebaudiane also a...The worldwide use of Stevia rebaudiane as substitute sweetener for sugar, increases every year. Stevia rebaudiane is not involved in the insulin mechanism and as so has no calories. This makes Stevia rebaudiane also a natural substitute for the more common synthetic substitutes for sugar. Various studies show that the plant Stevia rebaudiane contains substances with properties of antioxidant reagent, and as such can prevent antioxidation damage to DNA. [1] In this work protocols for extraction are developed and antioxidating activity tested. The aim was to find optimal conditions for the process, so the antioxidants in the Stevia plant were preserved. Methods used for testing: Redox-titrations and spectrophothometric methods. In all results Vitamin C was used as reference for the antioxidation activity. The results show that the Stevia rebaudiane plant is active as an antioxidant reagent; and that the extent of the antioxidant activity depends on the solvent and on the conditions of the extraction process. In addition there was an attend to measure sweetness of the Stevia rebaudiane plant extract as function of the extraction process.展开更多
文摘The worldwide use of Stevia rebaudiane as substitute sweetener for sugar, increases every year. Stevia rebaudiane is not involved in the insulin mechanism and as so has no calories. This makes Stevia rebaudiane also a natural substitute for the more common synthetic substitutes for sugar. Various studies show that the plant Stevia rebaudiane contains substances with properties of antioxidant reagent, and as such can prevent antioxidation damage to DNA. [1] In this work protocols for extraction are developed and antioxidating activity tested. The aim was to find optimal conditions for the process, so the antioxidants in the Stevia plant were preserved. Methods used for testing: Redox-titrations and spectrophothometric methods. In all results Vitamin C was used as reference for the antioxidation activity. The results show that the Stevia rebaudiane plant is active as an antioxidant reagent; and that the extent of the antioxidant activity depends on the solvent and on the conditions of the extraction process. In addition there was an attend to measure sweetness of the Stevia rebaudiane plant extract as function of the extraction process.