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钾通道阻滞剂对多发性硬化患者细胞因子分泌的影响 被引量:2
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作者 任晓暾 吴卫平 +1 位作者 黄德晖 徐全刚 《中国神经免疫学和神经病学杂志》 CAS 2011年第5期339-342,共4页
目的研究多发性硬化(multiple sclerosis,MS)患者髓鞘反应性CD4+T淋巴细胞分泌γ干扰素(INF-γ)和白细胞介素-4(IL-4)的水平,并探讨钾通道阻滞剂对其分泌的影响。方法采用酶联免疫斑点方法(ELISPOT)对12例急性期MS患者、12例缓解期MS患... 目的研究多发性硬化(multiple sclerosis,MS)患者髓鞘反应性CD4+T淋巴细胞分泌γ干扰素(INF-γ)和白细胞介素-4(IL-4)的水平,并探讨钾通道阻滞剂对其分泌的影响。方法采用酶联免疫斑点方法(ELISPOT)对12例急性期MS患者、12例缓解期MS患者(经INF--β1b治疗)和10名健康对照的CD4+T淋巴细胞在有无髓鞘抗原和钾通道阻滞剂作用下分泌细胞因子INF-γ和IL-4的变化进行比较。结果 MS急性期外周血CD4+T细胞以分泌IFN-γ为主;MS急性期及缓解期的CD4+T淋巴细胞经髓鞘碱性蛋白(MBP)刺激后分泌IFN-γ的水平较未加抗原组均增高(P<0.05),加入Kv1.3通道阻滞剂海葵毒素(Stichodactyla helianthustoxin,SHK)后,MS急性期和缓解期的MBP反应CD4+T淋巴细胞IFN-γ分泌明显减低(P<0.05),而对IL-4无明显影响。结论 Kv1.3钾通道阻滞剂SHK能减少MS患者MBP反应CD4+T细胞分泌IFN-γ,提示髓鞘反应性CD4+T细胞上Kv1.3通道有可能作为治疗MS的新靶点。 展开更多
关键词 多发性硬化 Kv1.3钾通道 海葵毒素 细胞因子 髓鞘碱性蛋白
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Changes of Cellular Superficial Configuration of Symbiotic Algae During Cultivation from Two Anemones Found in the South China Sea 被引量:1
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作者 ZHU Baohua PAN Kehou WANG Guangce 《Journal of Ocean University of China》 SCIE CAS 2008年第1期89-92,共4页
Symbiotic algae from two anemones, Radianthus macrodactylus and Stichodactyla mertensii, found in the South China Sea, were cultivated in ASP-8A medium in this study. Changes of superficial configuration of symbiotic ... Symbiotic algae from two anemones, Radianthus macrodactylus and Stichodactyla mertensii, found in the South China Sea, were cultivated in ASP-8A medium in this study. Changes of superficial configuration of symbiotic algae during the cultivation were studied by means of a microscope and a scanning electron microscope (SEM). A number of small cavities appeared on the sur- faces of symbiotic algae after they were cultivated for 10 h. The cavities enlarged and the cell contents were lost with extended culti- vation. Our data suggested that the presence of cavities on symbiotic algae surfaces may be one of the main reasons for failure to culture symbiotic algae in an artificial medium. 展开更多
关键词 Radianthus macrodactylus stichodactyla mertensii symbiotic algae superficial configuration
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