Membranous nephropathy(MN)is a glomerulonephritis that has an C5b-9-mediated podocyte injury as its main feature.Stigma maydis polysaccharides(SMP)demonstrate a protective role against podocyte injury in vivo.However,...Membranous nephropathy(MN)is a glomerulonephritis that has an C5b-9-mediated podocyte injury as its main feature.Stigma maydis polysaccharides(SMP)demonstrate a protective role against podocyte injury in vivo.However,their in vitro effects remain unknown.The aim of the present study was to verify the potential protective effect of acidic SMP(ASMP)and neutral SMP(NSMP)on podocytes in vitro.Morphological changes and cell migration were examined by inverted microscope,changes in reactive oxygen species level and apoptosis were detected by fluorescence microscope,while the cell cycle arrest were measured by flow cytometry.The expression of the phosphoinositide 3-kinases(PI3K),and Akt protein were detected by Western blot technique.This study showed that oxidative stress,migration,cell cycle arrest,and apoptosis were all significantly suppressed following pretreatment with either of the SMP,however ASMP showed greater effects overall.In conclusion,ASMP could potentially be developed as a functional food to protect podocytes from sC5b-9-induced oxidative stress and apoptosis injury.展开更多
目的:观察不同浓度的玉米须多糖(stigm a m ayd is po lysaccharide SM PS)对肝癌SMM C-7721细胞作用的影响。方法:将玉米须多糖以不同浓度和时间作用于肝癌SMM C-7721细胞,采用M TT比色法观察细胞毒性;应用HE染色法观察凋亡细胞的形态...目的:观察不同浓度的玉米须多糖(stigm a m ayd is po lysaccharide SM PS)对肝癌SMM C-7721细胞作用的影响。方法:将玉米须多糖以不同浓度和时间作用于肝癌SMM C-7721细胞,采用M TT比色法观察细胞毒性;应用HE染色法观察凋亡细胞的形态;原位末端标记法(TUNEL法)检测细胞凋亡率。结果:玉米须多糖以剂量依赖和时间依赖的方式抑制SMM C-7721细胞的生长。HE染色观察到凋亡细胞的形态学改变。结论:玉米须多糖抑制SMM C-7721细胞的增殖,诱导细胞凋亡。为临床治疗肝癌提供理论依据。展开更多
基金financial support provided by the National Key Research and Development Program of China(2017YFD0400504).
文摘Membranous nephropathy(MN)is a glomerulonephritis that has an C5b-9-mediated podocyte injury as its main feature.Stigma maydis polysaccharides(SMP)demonstrate a protective role against podocyte injury in vivo.However,their in vitro effects remain unknown.The aim of the present study was to verify the potential protective effect of acidic SMP(ASMP)and neutral SMP(NSMP)on podocytes in vitro.Morphological changes and cell migration were examined by inverted microscope,changes in reactive oxygen species level and apoptosis were detected by fluorescence microscope,while the cell cycle arrest were measured by flow cytometry.The expression of the phosphoinositide 3-kinases(PI3K),and Akt protein were detected by Western blot technique.This study showed that oxidative stress,migration,cell cycle arrest,and apoptosis were all significantly suppressed following pretreatment with either of the SMP,however ASMP showed greater effects overall.In conclusion,ASMP could potentially be developed as a functional food to protect podocytes from sC5b-9-induced oxidative stress and apoptosis injury.
文摘目的:观察不同浓度的玉米须多糖(stigm a m ayd is po lysaccharide SM PS)对肝癌SMM C-7721细胞作用的影响。方法:将玉米须多糖以不同浓度和时间作用于肝癌SMM C-7721细胞,采用M TT比色法观察细胞毒性;应用HE染色法观察凋亡细胞的形态;原位末端标记法(TUNEL法)检测细胞凋亡率。结果:玉米须多糖以剂量依赖和时间依赖的方式抑制SMM C-7721细胞的生长。HE染色观察到凋亡细胞的形态学改变。结论:玉米须多糖抑制SMM C-7721细胞的增殖,诱导细胞凋亡。为临床治疗肝癌提供理论依据。
