Stigma maydis polysaccharides protect podocyte injury by reducing oxidative stress and apoptosis

Membranous nephropathy(MN)is a glomerulonephritis that has an C5b-9-mediated podocyte injury as its main feature.Stigma maydis polysaccharides(SMP)demonstrate a protective role against podocyte injury in vivo.However,their in vitro effects remain unknown.The aim of the present study was to verify the potential protective effect of acidic SMP(ASMP)and neutral SMP(NSMP)on podocytes in vitro.Morphological changes and cell migration were examined by inverted microscope,changes in reactive oxygen species level and apoptosis were detected by fluorescence microscope,while the cell cycle arrest were measured by flow cytometry.The expression of the phosphoinositide 3-kinases(PI3K),and Akt protein were detected by Western blot technique.This study showed that oxidative stress,migration,cell cycle arrest,and apoptosis were all significantly suppressed following pretreatment with either of the SMP,however ASMP showed greater effects overall.In conclusion,ASMP could potentially be developed as a functional food to protect podocytes from sC5b-9-induced oxidative stress and apoptosis injury.

玉米须绞股蓝复合提取总黄酮工艺及其降压片的制备

以玉米须(stigma maydis)、绞股蓝(Gynostemma pentaphyllum)(1∶1)为提取原料,总黄酮含量为考察指标,通过提取溶剂、乙醇体积分数、提取温度、提取时间、料液比和提取次数等单因素考察对复合总黄酮含量的影响,再通过正交试验优化总黄酮的提取工艺,最后将优化提取的总黄酮过D101大孔吸附树脂精制后制备成降压片。结果表明,复方总黄酮的最佳提取工艺为乙醇体积分数50%,料液比1∶10,50℃下提取3.5 h。制备的片剂质量(片重差异±5.1%,脆碎度0.83%,崩解时限2.41 min)符合《中华人民共和国药典》(2020版)片剂的有关规定,并且降压片能降低SHR大鼠血压。