The role of RhoA in the migration of endothelial cells stimulated by interleukin-8

The development and maintenance of an abundant blood supply is essential for the healing of wound and neoplastic tissues.Angiogenesis,the development of new vessels from adjacent capillary networks,plays an important role in physiologic and as well as in

H2 Stimulated Raman Scattering in a Multi-pass Cell with a Herriott Configuration

Stimulated raman scattering （SRS） is an effective method for expanding the spectral range of high power lasers, especially in the regime of near IR and middle IR. We report the SRS of high pressure H2 with a multiple-pass cell configuration. The SRS with the multiple-pass cell configuration is found to be very efficient for reduction of threshold of the first Stokes （S1）. Due to the coherent SRS （CSRS） process, the multiple-pass cell configuration is more effective for reduction of the threshold for the second Stokes （S2） SRS and for increasing the conversion efficiency of S2. This contributes to the relatively low conversion efficiency of S1 for the multiple-pass cell configuration. Multiple-pass cell SRS is also found to be very effective for improving the beam quality and the stability of S1.

Retinal ganglion cells regenerate long-distance axons through neural activity stimulation and find their way back to the brain

Human central nerve system(CNS)is an extremely complex and delicate structure.While regeneration is possible in some reptiles and fish CNS,the regeneration capacity seems completely lost in adult mammals.Therefore,the classic concept is that once neurons in mammal

Delayed peripheral nerve repair: methods, including surgical ‘cross-bridging' to promote nerve regeneration

Despite the capacity of Schwann cells to support peripheral nerve regeneration, functional recovery after nerve injuries is frequently poor, especially for proximal injuries that require regenerating axons to grow over long distances to reinnervate distal targets. Nerve transfers, where small fascicles from an adjacent intact nerve are coapted to the nerve stump of a nearby denervated muscle, allow for functional return but at the expense of reduced numbers of innervating nerves. A 1-hour period of 20 Hz electrical nerve stimulation via electrodes proximal to an injury site accelerates axon outgrowth to hasten target reinnervation in rats and humans, even after delayed surgery. A novel strategy of enticing donor axons from an otherwise intact nerve to grow through small nerve grafts（cross-bridges） into a denervated nerve stump, promotes improved axon regeneration after delayed nerve repair. The efficacy of this technique has been demonstrated in a rat model and is now in clinical use in patients undergoing cross-face nerve grafting for facial paralysis. In conclusion, brief electrical stimulation, combined with the surgical technique of promoting the regeneration of some donor axons to ‘protect＇ chronically denervated Schwa nn cells, improves nerve regeneration and, in turn, functional outcomes in the management of peripheral nerve injuries.

Quantitative mitochondrial proteome of pancreatic INS-1β cells stimulated with prolonged high glucose using SILAC

Most type 2 diabetics are accompanied with deficiency of insulin secretion and hyperglycemia. It has reported that glucose-stimulated insulin secretion of β cell (GSIS)

Highly sensitive electrochemical detection of living cells based on diamond microelectrode arrays

We designed boron-doped nanocrystalline diamond microelectrode arrays（BNCD-MEAs） with 16 channels for the bioanalysis of multicellular samples, which could be readily adapted for a highly sensitive detection of H2 O2[2_TD$IF]released from stimulated cells by ascorbic acid（AA）. Our observations demonstrated that the as-prepared diamond microelectrode arrays could be utilized to distinguish cancer cells from normal cells, and the amperometric study showed the considerable differences in the currents, indicating that the related Hep G2 cancer cells could release more H2 O2 than that of L02 normal cells. This supports the possibility to use diamond-based MEAs for rapid cancer cell detection in future clinic applications.

DNA-based artificial dendritic cells for in situ cytotoxic T cell stimulation and immunotherapy

In immunotherapy,ex vivo stimulation of T cells requires significant resources and effort.Here,we report artificial dendritic cell-mimicking DNA microflowers(DM)for programming T cell stimulation in situ.To mimic dendritic cells,DNA-based artificial dendritic microflowers were constructed,surface-coated with polydopamine,and further modified with anti-CD3 and anti-CD28 antibodies to yield antibody-modified DM(DM-A).The porous structure of DM-A allowed entrapment of the T cell-stimulating cytokine,ineterleukin-2,yielding interleukin-2-loaded DM-A(DM-AI).For comparison,polystyrene microparticles coated with polydopamine and modified with anti-CD3 and anti-CD28 antibodies(PS-A)were used.Compared to PS-A,DM-AI showed significantly greater contact with T cell surfaces.DM-AI provided the highest ex vivo expansion of cytotoxic T cells.Local injection of DM-AI to tumor tissues induced the recruitment of T cells and expansion of cytotoxic T cells in tumor microenvironments.Unlike the other groups,model animals injected with DM-AI did not exhibit growth of primary tumors.Treatment of mice with DM-AI also protected against growth of a rechallenged distant tumor,and thus prevented tumor recurrence in this model.DM-AI has great potential for programmed stimulation of CD8+T cells.This concept could be broadly extended for the programming of specific T cell stimulation profiles.

Potential advantages of a combination of Chinese Medicine and bone marrow mesenchymal stem cell transplantation for removing blood stasis and stimulating neogenesis during ischemic stroke treatment

Combined treatment of ischemic stroke with Chinese medicine and exogenous bone marrow mesenchymal stem cell(BMSC) transplantation may improve the removal of blood stasis and stimulation of neogenesis.Chinese medicines that remove blood stasis not only promote blood circulation but also calm the endopathic wind,remove heat,resolve phlegm,remove toxic substances and strengthen body resistance.The medicinal targeting effect of Chinese medicine can promote the homing of BMSCs,and the synergistic therapeutic effects of drugs can contribute to BMSC differentiation.As such,exogenous BMSC transplantation has potential advantages for neogenesis.Chinese medicines and exogenous BMSCs provide complementary functions for the removal of blood stasis and stimulation of neogenesis.Therefore,a combination of Chinese medicine and transplantation of exogenous BMSCs may be particularly suited to ischemic stroke treatment.