A highly sensitive stopped-flow spectrophotometry kinetic method was proposed for quantification phenoxyl radicals based on their accelerating effect on the oxidation of nicotinamide adenine dinucleotide （NADH）. Phe...A highly sensitive stopped-flow spectrophotometry kinetic method was proposed for quantification phenoxyl radicals based on their accelerating effect on the oxidation of nicotinamide adenine dinucleotide （NADH）. Phenoxyl radicals generated from as low as 1×10^-8 mol/L 2,4-DCP can be readily detected with the proposed method and the detecting limit was 2.5×10^-9 mol/L.展开更多
The characteristic of Myoglobin as mimetic enzyme was studied and applied in the determination of HeO2 with o-phenylenediamine as substrate. The absorbanee obtained at 40 ℃ was found to be proportional to the concent...The characteristic of Myoglobin as mimetic enzyme was studied and applied in the determination of HeO2 with o-phenylenediamine as substrate. The absorbanee obtained at 40 ℃ was found to be proportional to the concentration of H2O2 in the range of 2.0×10^-5- 2.0×10^-7 mol/L, with a correlation coefficient of 0.999 1. The proposed meth od is simple and highly sensitive to a detection limit of 2.7 × 10^-8 mol/L. The relative standard deviations were within 5% for the determination of different concentrations of H2O2. Satisfied results were obtained in the determination of H2O2 in rainwater by this method, and the recoveries were at the range of 97.2%-105.4%.展开更多
基金The authors gratefully acknowledge financial support from the National Natural Science Foundation of China （No. 20275027 and No. 20377032）.
文摘A highly sensitive stopped-flow spectrophotometry kinetic method was proposed for quantification phenoxyl radicals based on their accelerating effect on the oxidation of nicotinamide adenine dinucleotide （NADH）. Phenoxyl radicals generated from as low as 1×10^-8 mol/L 2,4-DCP can be readily detected with the proposed method and the detecting limit was 2.5×10^-9 mol/L.
基金Supported by the National Natural Science Foun-dation of China (20275027) the Natural Science Foundation ofHubei Province (2004ABA182) .
文摘The characteristic of Myoglobin as mimetic enzyme was studied and applied in the determination of HeO2 with o-phenylenediamine as substrate. The absorbanee obtained at 40 ℃ was found to be proportional to the concentration of H2O2 in the range of 2.0×10^-5- 2.0×10^-7 mol/L, with a correlation coefficient of 0.999 1. The proposed meth od is simple and highly sensitive to a detection limit of 2.7 × 10^-8 mol/L. The relative standard deviations were within 5% for the determination of different concentrations of H2O2. Satisfied results were obtained in the determination of H2O2 in rainwater by this method, and the recoveries were at the range of 97.2%-105.4%.
