本文对草莓轻型黄边病毒(Strawberry mild yellow edge virus,SMYEV)的2种分子检测技术进行了比较研究。结果表明,多重PCR技术以草莓actin为内参基因,完全排除在实验中杂质与操作的影响。Real time PCR技术具有更高的灵敏度和可靠性,可...本文对草莓轻型黄边病毒(Strawberry mild yellow edge virus,SMYEV)的2种分子检测技术进行了比较研究。结果表明,多重PCR技术以草莓actin为内参基因,完全排除在实验中杂质与操作的影响。Real time PCR技术具有更高的灵敏度和可靠性,可以定量检测出样本中病毒粒子的含量。结果表明,Real time PCR的灵敏度是普通PCR的100倍以上,而多重PCR的检测成本更经济。所以Real time PCR技术将用于引进品种的检疫,而多重PCR技术将用于大规模的脱毒苗的检验。展开更多
The 930 bp segment in 3’ terminal region of Strawberry mild yellow edge virus(SMYEV)genome was amplified by 3’ rapid amplification of cDNA ends(RACE).Ten Chinese isolates were sequenced,and 7 of them were the same.N...The 930 bp segment in 3’ terminal region of Strawberry mild yellow edge virus(SMYEV)genome was amplified by 3’ rapid amplification of cDNA ends(RACE).Ten Chinese isolates were sequenced,and 7 of them were the same.Nucleotide and amino acid identities and phylogenesis were analyzed between Chinese isolates and 24 isolates from other regions of the world.Sequence analysis of the 878 nt stretch within 3’ terminal region of SMYEV genome showed that nucleotide acid identities ranged from 79.5% to 100%,deduced amino acid sequences of coat protein gene identity were 86.4% to 100%.Phylogenetic analysis showed that all isolates of SMYEV fell into four clades.To a certain extent,the clades were related with the geological distribution of SMYEV.Chinese isolates SY01 and SY04 lay in the same clade with European and American isolates,but formed a small separate branch.Isolates SY03 and SY02,derived from Fragaria×ananassa cv.Changhong-2 and F.pentaphylla respectively,had a far relationship with other isolates and fell into one clade.They were likely to be the special isolates that existed only in China.展开更多
文摘本文对草莓轻型黄边病毒(Strawberry mild yellow edge virus,SMYEV)的2种分子检测技术进行了比较研究。结果表明,多重PCR技术以草莓actin为内参基因,完全排除在实验中杂质与操作的影响。Real time PCR技术具有更高的灵敏度和可靠性,可以定量检测出样本中病毒粒子的含量。结果表明,Real time PCR的灵敏度是普通PCR的100倍以上,而多重PCR的检测成本更经济。所以Real time PCR技术将用于引进品种的检疫,而多重PCR技术将用于大规模的脱毒苗的检验。
文摘The 930 bp segment in 3’ terminal region of Strawberry mild yellow edge virus(SMYEV)genome was amplified by 3’ rapid amplification of cDNA ends(RACE).Ten Chinese isolates were sequenced,and 7 of them were the same.Nucleotide and amino acid identities and phylogenesis were analyzed between Chinese isolates and 24 isolates from other regions of the world.Sequence analysis of the 878 nt stretch within 3’ terminal region of SMYEV genome showed that nucleotide acid identities ranged from 79.5% to 100%,deduced amino acid sequences of coat protein gene identity were 86.4% to 100%.Phylogenetic analysis showed that all isolates of SMYEV fell into four clades.To a certain extent,the clades were related with the geological distribution of SMYEV.Chinese isolates SY01 and SY04 lay in the same clade with European and American isolates,but formed a small separate branch.Isolates SY03 and SY02,derived from Fragaria×ananassa cv.Changhong-2 and F.pentaphylla respectively,had a far relationship with other isolates and fell into one clade.They were likely to be the special isolates that existed only in China.