To improve the efficiency of fetching and transplanting seedlings for the mechanization of strawberry planting,an integrated transplanting mechanism was designed with protruding,fetching and planting performance to ac...To improve the efficiency of fetching and transplanting seedlings for the mechanization of strawberry planting,an integrated transplanting mechanism was designed with protruding,fetching and planting performance to achieve rapid fetching and pushing bowl movements.According to the working principle of the slewing mechanism,a kinematics model and the optimization goal were established,respectively.Based on visual auxiliary analysis software,optimal parameters were obtained.A three-dimensional model was established to obtain a simulation trajectory by means of a virtual simulation design analysis.Three-dimensional printing technology was used to manufacture the test prototype,and the actual working trajectories of the test prototype were extracted using high-speed photography technology,which verified the consistency of the actual trajectory with the theoretical and simulated trajectories.A prototype transplanting experiment was performed with the success rate of seedling extraction of 91.2%and excellent planting rate of 82.8%,which met the requirements for integrated strawberry harvesting,planting and transplanting.The experimental results verified the correctness and feasibility of the design of integrated transplanting mechanism.展开更多
Shoot-tip culture of strawberry is the most important way to get virus-free plants.Moreover,detoxification and rapid propagation are the key techniques for producing high-quality seeds and seedlings massively and rapi...Shoot-tip culture of strawberry is the most important way to get virus-free plants.Moreover,detoxification and rapid propagation are the key techniques for producing high-quality seeds and seedlings massively and rapidly and achieving the high-quality,high-yield and high-efficiency development of strawberry industry.We have recently produced virus-free strawberry seedlings by combing tissue culture technology and stolon-reproduction method and this technology has been widely used.In addition,a relatively perfect three breeding system of virus-free strawberry seedlings was established after production practices,guaranteeing the supply of high-quality seeds and seedlings and technical support for strawberry industry in corps.展开更多
With Arena strawberry as experimental materials, heat treatments combined with shoot tip culture were used to explore their detoxification effects. The results indicated that if not heated, splitting shoot tips direct...With Arena strawberry as experimental materials, heat treatments combined with shoot tip culture were used to explore their detoxification effects. The results indicated that if not heated, splitting shoot tips directly could not completely remove viruses. On the other hand, it will be obviously improved after shoot tips are heated. Incubators heat treatment (inconstant temperature) with shoot tip culture, constant temperature water bath incubation process with meristem-tip culture, hot air treatment (constant temperature) with apical meristem culture were three methods tested in searching for virus-free of Arena strawberry in this experiment. And their virus-free rates were 78.9%, 86.0% and 50.0% respectively. Of the three methods, the second, constant temperature water bath process with shoot tip culture, with 78.7% survival, is the best. Thus, it can be inferred that constant temperature water bath treatment combined with shoot tip culture might be the most appropriate method to remove viruses of Arena strawberry.展开更多
[ Objective] This study aimed to investigate the tissue culture and rapid propagation techniques of Benihoppe strawberry. [ Method] Shoot tips of new stolons of Benihoppe strawberry were used as experimental materials...[ Objective] This study aimed to investigate the tissue culture and rapid propagation techniques of Benihoppe strawberry. [ Method] Shoot tips of new stolons of Benihoppe strawberry were used as experimental materials to analyze the effects of media type, cytokinin type and concentration, carbon source type and concentration, and light intensity on tissue culture and propagation of Benihoppe strawberry. [ Result] MS was the optimal media for plantlet propagation. In the media containing 1.2 ing/L BA (with addition of 0.1 mg/L NAA), fresh weight, dry weight and propagation coefficient of strawberry plantlets reached the maxi- mum, which were 2.259 g, 0. 221 g and 12.4, respectively. The optimal carbon source was 30 g/L sucrose, and the optimal light intensity was 1 600 lx. [ Conclu- sion] The best media for tissue culture and rapid propagation of Benihoppe strawberry was MS + BA 1.2 mg/L + NAA 0. 1 mg/L + sucrose 30 g/L + agar 8 g/L. This study provided scientific basis for large-scale production of Benihoppe strawberry.展开更多
In this study, we employed a temporary immersion bioreactor(TIB)system for the micropropagation of strawberry seedlings. The TIB method and the conventional method for the micropropagation of strawberry seedlings were...In this study, we employed a temporary immersion bioreactor(TIB)system for the micropropagation of strawberry seedlings. The TIB method and the conventional method for the micropropagation of strawberry seedlings were compared in terms of explant propagation coefficient, seedling fresh weight,contents of anthocyanins and chlorophyll, and photosynthetic characteristics. The results showed that an inoculation density of 40 explants/L was suitable for the micropropagation of ’Benihope’ strawberry seedlings in the TIB. The propagation coefficient, fresh weight, photosynthetic rate, stomatal conductance, transpiration coefficient, and the contents of total anthocyanins and chlorophyll of seedlings micropropagated in the TIB were significantly higher than those of the seedlings micropropagated by the conventional method. In conclusion, the TIB method was superior to the conventional method in the micropropagation of strawberry seedlings.The negative reciprocity did not occur between anthocyanins and chlorophyll in strawberry seedlings cultured under forced ventilation such as TIB.展开更多
By employing temporary immersion bioreactor system(TIBs),we studied virus-free culture of seedlings from sugarcane varieties ROC16 and ROC22,from medium recipe,inoculation amount,sucrose concentration,and variety diff...By employing temporary immersion bioreactor system(TIBs),we studied virus-free culture of seedlings from sugarcane varieties ROC16 and ROC22,from medium recipe,inoculation amount,sucrose concentration,and variety difference. The results showed,using this method,that proliferation rate of ROC16 improved by 40 times,per flask generated about 800 plantlets; of ROC22 improved by 30 times,per flask generated about 400-600 plantlets. The results provided basis for using TIBs in rapid propagation of plantlets via tissue culture.展开更多
Virus-free sugarcane seedlings have improved biomass and sucrose content compared with ordinary seedlings, and sucrose invertases are key enzymes regulating sugarcane growth and sucrose accumulation. In this study, th...Virus-free sugarcane seedlings have improved biomass and sucrose content compared with ordinary seedlings, and sucrose invertases are key enzymes regulating sugarcane growth and sucrose accumulation. In this study, the differences in the expression levels of 3 invertase genes, CWI, SAI and NI, between virus- free and ordinary sugarcane seedlings were analyzed. Compared with ordinary sugarcane plants, the expression of CWI was mainly up-regulated in immature leaves and stems at elongation stage and leaves and immature internodes at maturation stage, and especially, greatly up-regulated in immature interuedes at maturation stage of virus-free plants. The expression of SAI and NI were mainly up-regnlated in leaves and immature internedes of virus-free plants at maturation stage, which might be beneficial to sugar accumulation and rapid utilization of monosaccharide in the stalks of virus-free plants. It is further indicated that virus-free treatment could significantly improve the expression of sucrose invertases at late growth period, and might facilitate the increase of plant biomass.展开更多
Strawberry is rich in nutrition,which is the only fresh fruit in winter in southern Xinjiang,with good prospect of stereoscopic cultivation.In this paper,the key techniques such as stereoscopic cultivation pattern,the...Strawberry is rich in nutrition,which is the only fresh fruit in winter in southern Xinjiang,with good prospect of stereoscopic cultivation.In this paper,the key techniques such as stereoscopic cultivation pattern,the combination of different modes,the substrate of strawberry cultivation,the drip irrigation system,the formula of nutrient solution,the selection of varieties,the propagation of seed and seedling,the induction of flower buds,the planting and management of plants,and the green control of diseases and insect pests are introduced,and greenhouse space and solar energy are fully used to maximize the economic and social benefits of strawberry farming combined with tourism.The results will further promote the quality and efficiency of strawberry industry in southern Xinjiang,and provide a reference for strawberry growers.展开更多
基金Supported by the National Natural Science Foundation of China Youth Fund Project(52005221)"the 13th Five-Year"National Key Research and Development Program:High-speed Planting Technology and Equipment Research and Development(2017YFD0700800)。
文摘To improve the efficiency of fetching and transplanting seedlings for the mechanization of strawberry planting,an integrated transplanting mechanism was designed with protruding,fetching and planting performance to achieve rapid fetching and pushing bowl movements.According to the working principle of the slewing mechanism,a kinematics model and the optimization goal were established,respectively.Based on visual auxiliary analysis software,optimal parameters were obtained.A three-dimensional model was established to obtain a simulation trajectory by means of a virtual simulation design analysis.Three-dimensional printing technology was used to manufacture the test prototype,and the actual working trajectories of the test prototype were extracted using high-speed photography technology,which verified the consistency of the actual trajectory with the theoretical and simulated trajectories.A prototype transplanting experiment was performed with the success rate of seedling extraction of 91.2%and excellent planting rate of 82.8%,which met the requirements for integrated strawberry harvesting,planting and transplanting.The experimental results verified the correctness and feasibility of the design of integrated transplanting mechanism.
文摘Shoot-tip culture of strawberry is the most important way to get virus-free plants.Moreover,detoxification and rapid propagation are the key techniques for producing high-quality seeds and seedlings massively and rapidly and achieving the high-quality,high-yield and high-efficiency development of strawberry industry.We have recently produced virus-free strawberry seedlings by combing tissue culture technology and stolon-reproduction method and this technology has been widely used.In addition,a relatively perfect three breeding system of virus-free strawberry seedlings was established after production practices,guaranteeing the supply of high-quality seeds and seedlings and technical support for strawberry industry in corps.
文摘With Arena strawberry as experimental materials, heat treatments combined with shoot tip culture were used to explore their detoxification effects. The results indicated that if not heated, splitting shoot tips directly could not completely remove viruses. On the other hand, it will be obviously improved after shoot tips are heated. Incubators heat treatment (inconstant temperature) with shoot tip culture, constant temperature water bath incubation process with meristem-tip culture, hot air treatment (constant temperature) with apical meristem culture were three methods tested in searching for virus-free of Arena strawberry in this experiment. And their virus-free rates were 78.9%, 86.0% and 50.0% respectively. Of the three methods, the second, constant temperature water bath process with shoot tip culture, with 78.7% survival, is the best. Thus, it can be inferred that constant temperature water bath treatment combined with shoot tip culture might be the most appropriate method to remove viruses of Arena strawberry.
文摘[ Objective] This study aimed to investigate the tissue culture and rapid propagation techniques of Benihoppe strawberry. [ Method] Shoot tips of new stolons of Benihoppe strawberry were used as experimental materials to analyze the effects of media type, cytokinin type and concentration, carbon source type and concentration, and light intensity on tissue culture and propagation of Benihoppe strawberry. [ Result] MS was the optimal media for plantlet propagation. In the media containing 1.2 ing/L BA (with addition of 0.1 mg/L NAA), fresh weight, dry weight and propagation coefficient of strawberry plantlets reached the maxi- mum, which were 2.259 g, 0. 221 g and 12.4, respectively. The optimal carbon source was 30 g/L sucrose, and the optimal light intensity was 1 600 lx. [ Conclu- sion] The best media for tissue culture and rapid propagation of Benihoppe strawberry was MS + BA 1.2 mg/L + NAA 0. 1 mg/L + sucrose 30 g/L + agar 8 g/L. This study provided scientific basis for large-scale production of Benihoppe strawberry.
基金Supported by the Innovation Platform Open Fund of Education Department in Hunan Province (18K100)。
文摘In this study, we employed a temporary immersion bioreactor(TIB)system for the micropropagation of strawberry seedlings. The TIB method and the conventional method for the micropropagation of strawberry seedlings were compared in terms of explant propagation coefficient, seedling fresh weight,contents of anthocyanins and chlorophyll, and photosynthetic characteristics. The results showed that an inoculation density of 40 explants/L was suitable for the micropropagation of ’Benihope’ strawberry seedlings in the TIB. The propagation coefficient, fresh weight, photosynthetic rate, stomatal conductance, transpiration coefficient, and the contents of total anthocyanins and chlorophyll of seedlings micropropagated in the TIB were significantly higher than those of the seedlings micropropagated by the conventional method. In conclusion, the TIB method was superior to the conventional method in the micropropagation of strawberry seedlings.The negative reciprocity did not occur between anthocyanins and chlorophyll in strawberry seedlings cultured under forced ventilation such as TIB.
基金Supported by Youth Science Foundation of Guangxi ( Guikeqing0832060)S&T Development Project from Guangxi Academy of Agricultural Sciences(2006006)~~
文摘By employing temporary immersion bioreactor system(TIBs),we studied virus-free culture of seedlings from sugarcane varieties ROC16 and ROC22,from medium recipe,inoculation amount,sucrose concentration,and variety difference. The results showed,using this method,that proliferation rate of ROC16 improved by 40 times,per flask generated about 800 plantlets; of ROC22 improved by 30 times,per flask generated about 400-600 plantlets. The results provided basis for using TIBs in rapid propagation of plantlets via tissue culture.
基金Supported by"863"Program(2013AA102604-1)Natural Science Foundation of Hainan Province(20163124)+1 种基金Basal Research Fund for Central Public-interest Scientific Institute(ITBB140503)Earmarked Fund for China Agriculture Research System(CARS-20-2-5)
文摘Virus-free sugarcane seedlings have improved biomass and sucrose content compared with ordinary seedlings, and sucrose invertases are key enzymes regulating sugarcane growth and sucrose accumulation. In this study, the differences in the expression levels of 3 invertase genes, CWI, SAI and NI, between virus- free and ordinary sugarcane seedlings were analyzed. Compared with ordinary sugarcane plants, the expression of CWI was mainly up-regulated in immature leaves and stems at elongation stage and leaves and immature internodes at maturation stage, and especially, greatly up-regulated in immature interuedes at maturation stage of virus-free plants. The expression of SAI and NI were mainly up-regnlated in leaves and immature internedes of virus-free plants at maturation stage, which might be beneficial to sugar accumulation and rapid utilization of monosaccharide in the stalks of virus-free plants. It is further indicated that virus-free treatment could significantly improve the expression of sucrose invertases at late growth period, and might facilitate the increase of plant biomass.
基金Supported by Science and Technology Project of the First Division of Xinjiang Production and Construction Corps"Construction and Technology Demonstration of Strawberry Stereoscopic Cultivation Expo Park"(2019NY01)"Three Zones"Science and Technology Talents Project of Xinjiang Production and Construction Corps。
文摘Strawberry is rich in nutrition,which is the only fresh fruit in winter in southern Xinjiang,with good prospect of stereoscopic cultivation.In this paper,the key techniques such as stereoscopic cultivation pattern,the combination of different modes,the substrate of strawberry cultivation,the drip irrigation system,the formula of nutrient solution,the selection of varieties,the propagation of seed and seedling,the induction of flower buds,the planting and management of plants,and the green control of diseases and insect pests are introduced,and greenhouse space and solar energy are fully used to maximize the economic and social benefits of strawberry farming combined with tourism.The results will further promote the quality and efficiency of strawberry industry in southern Xinjiang,and provide a reference for strawberry growers.