Changes of Activities in NAD Kinase and NADP PhosphataseDuring Ripening and Senescence of Tomatoand Strawberry Fruits

Activities of NAD kinase(NADK)and NADP phosphatase and relationship between the two enzymes and temperature, respiration, ethylene production and trifluoperazine(TFP) were studied during ripening and senescence of strawberry and tomato fruits after harvest at 4℃ and 20℃. The activity of NAD kinase in strawberry decreased slowly during first four days, then increased gradually. The NADP phosphatase activity increased at the second day, decreased the next day,then increased again. In tomato fruit, the activities of NAD kinase and NADP phosphatase increased at the second day, decreased with the ripening and senescence of the fruit. The change trend of NAD kinase and respiration in the two fruits were similar, the same were NADP phosphatase and ethylene production. TFP enhanced the activity of NAD kinase and had little effect on NADP phosphatase. Low temperature(4℃) activated the NAD kinase and reduced the activity of NADP phosphatase. These results indicated that the NAD kinase and NADP phosphatase were related to the ripening and senescence of strawberry and tomato fruits. The activation of NAD kinase probably postponed the ripening and senescence of the fruits.

草莓和番茄果实采后NAD激酶、NADP磷酸酶活性变化的研究

研究了草莓和番茄果实采后在 4℃和 2 0℃下 ,NAD激酶 (NADK)、NADP磷酸酶活性变化及其与果实呼吸、乙烯释放率、钙调素拮抗剂三氟啦嗪 (Trifluoperazine ,TFP)的关系。结果表明 ,两种果实的NADK、NADP磷酸酶活性变化趋势存在显著差异 ,草莓果实采后NADK活性先下降、后上升 ,NADP磷酸酶活性出现第 1个峰后继续缓慢上升 ,而番茄果实NADK活性先上升、后下降 ,NADP磷酸酶活性在出现第 1个峰后继续下降 ;两种果实NADK活性变化与呼吸变化趋势相似 ,NADP磷酸酶变化与乙烯释放率变化趋势相似 ;低温激活NADK ,抑制NADP磷酸酶 ,TFP对NADK有激活作用 ,对NADP磷酸酶影响较小。表明果实后熟衰老过程与NAD激酶、NADP磷酸酶密切相关 ,激活NADK可能与延缓果实后熟衰老有关。