[Objective] This study aimed to explore the mutagenesis effects of N＋ ion beam implantation on Streptomyces a/bus and obtain high-yield salinomycin- producing mutant strain. [ Method ] Streptomyces a/bus strain S-11-...[Objective] This study aimed to explore the mutagenesis effects of N＋ ion beam implantation on Streptomyces a/bus and obtain high-yield salinomycin- producing mutant strain. [ Method ] Streptomyces a/bus strain S-11-04 was mutated with different doses of N ＋ implantation. The effects of low energy N ＊ implantation on the survival rate, colony morphology and salinomycin-producing ability were investigated. [ Result] The results showed that low energy N ＋ implantation can efficiently improve the positive mutation rate of Streptomyces albus; 13 mutant strains with high yield of salinomycin were isolated; to be specific, mutant strain N3- 6 has relatively good genetic stability with four continuous generations, and the titres of salinomycin were increased by 41% in the shake-flask culture and 20.5% in mass production compared with the control. [ Conclusion ] N ＋ ion beam irradiation is an effective method to obtain high-yield salinomycin-producing Streptomy- ces albus strain.展开更多
Dear Editor,Streptomyces can produce a large variety of secondary metabolites as a major source of anti-infective, antitumor or immune-suppressive agents widely applied in clinical treatment. Antibiotics-resistant bac...Dear Editor,Streptomyces can produce a large variety of secondary metabolites as a major source of anti-infective, antitumor or immune-suppressive agents widely applied in clinical treatment. Antibiotics-resistant bacteria are spreading at alarming rates.展开更多
为了筛选和鉴定ε-聚赖氨酸（ε-poly-lysine,ε-PL）产生菌,试验利用酸性品红从辽宁营口东部山区土壤中筛选到一株放线菌E301,采用薄层层析法分析发酵产物及其酸水解成分,水杨醛保护法分析产物酰胺键连接方式,红外光谱法对产物进行表征...为了筛选和鉴定ε-聚赖氨酸（ε-poly-lysine,ε-PL）产生菌,试验利用酸性品红从辽宁营口东部山区土壤中筛选到一株放线菌E301,采用薄层层析法分析发酵产物及其酸水解成分,水杨醛保护法分析产物酰胺键连接方式,红外光谱法对产物进行表征,通过形态观察、培养特征、生理生化特征并结合16S r DNA序列分析对菌种进行鉴定。结果表明：放线菌E301发酵产物与Dragendorff试剂、甲基橙试剂作用都有沉淀产生,薄层层析显示发酵产物的酸水解成分为单体赖氨酸,产物酰胺键连接方式为ε型,红外光谱图与标准ε-聚赖氨酸一致,形态学及分子生物学鉴定放线菌E301与白色链霉菌的同源性大于99%。说明放线菌E301为白色链霉菌Streptomyces albus。展开更多
文摘[Objective] This study aimed to explore the mutagenesis effects of N＋ ion beam implantation on Streptomyces a/bus and obtain high-yield salinomycin- producing mutant strain. [ Method ] Streptomyces a/bus strain S-11-04 was mutated with different doses of N ＋ implantation. The effects of low energy N ＊ implantation on the survival rate, colony morphology and salinomycin-producing ability were investigated. [ Result] The results showed that low energy N ＋ implantation can efficiently improve the positive mutation rate of Streptomyces albus; 13 mutant strains with high yield of salinomycin were isolated; to be specific, mutant strain N3- 6 has relatively good genetic stability with four continuous generations, and the titres of salinomycin were increased by 41% in the shake-flask culture and 20.5% in mass production compared with the control. [ Conclusion ] N ＋ ion beam irradiation is an effective method to obtain high-yield salinomycin-producing Streptomy- ces albus strain.
基金supported by grants from the Ministry of Science and Technology of China (2015CB150600)the National Natural Science Foundation of China (31571281 and 31771378)
文摘Dear Editor,Streptomyces can produce a large variety of secondary metabolites as a major source of anti-infective, antitumor or immune-suppressive agents widely applied in clinical treatment. Antibiotics-resistant bacteria are spreading at alarming rates.
文摘为了筛选和鉴定ε-聚赖氨酸（ε-poly-lysine,ε-PL）产生菌,试验利用酸性品红从辽宁营口东部山区土壤中筛选到一株放线菌E301,采用薄层层析法分析发酵产物及其酸水解成分,水杨醛保护法分析产物酰胺键连接方式,红外光谱法对产物进行表征,通过形态观察、培养特征、生理生化特征并结合16S r DNA序列分析对菌种进行鉴定。结果表明：放线菌E301发酵产物与Dragendorff试剂、甲基橙试剂作用都有沉淀产生,薄层层析显示发酵产物的酸水解成分为单体赖氨酸,产物酰胺键连接方式为ε型,红外光谱图与标准ε-聚赖氨酸一致,形态学及分子生物学鉴定放线菌E301与白色链霉菌的同源性大于99%。说明放线菌E301为白色链霉菌Streptomyces albus。
