[Objective] This study was conducted to investigate the biocontrol activity of Streptomyces corchorusii strain NF0919 and Bacillus subtilis D J-6 WP to grape downy mildew. [Methed] We determined the indoor toxicity of...[Objective] This study was conducted to investigate the biocontrol activity of Streptomyces corchorusii strain NF0919 and Bacillus subtilis D J-6 WP to grape downy mildew. [Methed] We determined the indoor toxicity of the supernatant of S. corchorusii strain NF0919, 1.0×1011 cfu/g B. subtilis D J-6 WP, mancozeb and dimethomorph on Plasmopara viticola by the leaf disc method, respectively, and a field efficacy trial was conducted. [Result] The results showed that the ECso values of the supernatant of strain NF0919, 1.0×1011cfu/g B. subtilis D J-6 WP, mancozeb and dimethomorph were 96.285 9, 86.603 8, 69.947 2 and 7.263 6 μg/ml, respec- tively. The values of field efficacy in preventive experiments for grape downy mildew on the 7th day after 2 times of spraying 20 times diluent of the supernatant of strain NF0919 and 1 000 times diluent of 1.0×1011 cfu/g B. subtilis D J-6 WP were 71.55% and 70.71%, respectively, and the values of field efficacy on the 14th day after the 2 times of fungicide application were 67.54% and 68.19%, respectively. The values of field efficacy in curative experiments on the 7th day after 2 times of spraying 20 times diluent of the supematant of strain NF0919 and 1 000 times diluent of 1.0×1011 cfu/g B. subtilis D J-6 WP were 59.72% and 56.07%, respectively, and the val- ues of field efficacy on the 14th day after the 2 times of fungicide application were 56.88% and 57.46%, respectively. The field efficacy values of the 2 tested biocon- trol agents were equivalent. The protective effect showed no significant difference between each of tested biocontrol agents and 300 times diluent of 50% mancozeb WP, but there was a significant difference in the efficacy between each of tested biocontrol agents and 200 times diluent of 40% dimethomorph SC. [Conclusion] The S. corchorusii strain NF0919 and B. subtilis D J-6 WP had certain biocontrol poten- tial to grape downy mildew and development value.展开更多
基金Supported by Science&Technology Specific Project for Enriching People and Strengthening County Economy in China(BN20156222)Jiangsu Agricultural Science and Technology Independent Innovation Fund(CX(14)2056)
文摘[Objective] This study was conducted to investigate the biocontrol activity of Streptomyces corchorusii strain NF0919 and Bacillus subtilis D J-6 WP to grape downy mildew. [Methed] We determined the indoor toxicity of the supernatant of S. corchorusii strain NF0919, 1.0×1011 cfu/g B. subtilis D J-6 WP, mancozeb and dimethomorph on Plasmopara viticola by the leaf disc method, respectively, and a field efficacy trial was conducted. [Result] The results showed that the ECso values of the supernatant of strain NF0919, 1.0×1011cfu/g B. subtilis D J-6 WP, mancozeb and dimethomorph were 96.285 9, 86.603 8, 69.947 2 and 7.263 6 μg/ml, respec- tively. The values of field efficacy in preventive experiments for grape downy mildew on the 7th day after 2 times of spraying 20 times diluent of the supernatant of strain NF0919 and 1 000 times diluent of 1.0×1011 cfu/g B. subtilis D J-6 WP were 71.55% and 70.71%, respectively, and the values of field efficacy on the 14th day after the 2 times of fungicide application were 67.54% and 68.19%, respectively. The values of field efficacy in curative experiments on the 7th day after 2 times of spraying 20 times diluent of the supematant of strain NF0919 and 1 000 times diluent of 1.0×1011 cfu/g B. subtilis D J-6 WP were 59.72% and 56.07%, respectively, and the val- ues of field efficacy on the 14th day after the 2 times of fungicide application were 56.88% and 57.46%, respectively. The field efficacy values of the 2 tested biocon- trol agents were equivalent. The protective effect showed no significant difference between each of tested biocontrol agents and 300 times diluent of 50% mancozeb WP, but there was a significant difference in the efficacy between each of tested biocontrol agents and 200 times diluent of 40% dimethomorph SC. [Conclusion] The S. corchorusii strain NF0919 and B. subtilis D J-6 WP had certain biocontrol poten- tial to grape downy mildew and development value.
