Objective To investigate whether pregnancy-induced hypertension （PIH） may increase oxidative stress in women with PIH, and to explore the mechanisms by which PIH may increase oxidative stress and potential free radi...Objective To investigate whether pregnancy-induced hypertension （PIH） may increase oxidative stress in women with PIH, and to explore the mechanisms by which PIH may increase oxidative stress and potential free radical damage. Methods Seventy women with PIH and seventy women with uncomplicated normotensive pregnancy （UNP） whose age, nutritional conditions, levels of hemoglobin and albumin were all matched, were enrolled in a randomized controlled trial. Their plasma concentrations of nitric oxide （NO）, vitamin C （VC）, vitamin E （VE）, and β-carotene （β-CAR） as well as their erythrocyte malondialdehyde （MDA）, and activities of superoxide dismutase （SOD）, catalase （CAT） and glutathione peroxidase （GPX） were determined by spcctrophotometry. Results Compared with average values of the above experimental parameters in the women with UNP, the average value of erythrocyte MDA in the women with PIH significantly increased （P〈0.0001）, while the average values of plasma NO, VC, VE, and β-CAR as well as those of erythrocyte SOD, CAT, and GPX in the women with PIH significantly decreased （P〈0.0005-0.0001）. The findings from partial correlation analysis （controlling for age） for 70 women with PIH showed that with elevated systolic blood pressure （SBP） and diastolic blood pressure （DBP）, MDA value gradually increased （P〈0.001）, and NO, VC, VE, β-CAR, SOD, CAT, and GPX values gradually decreased （P〈0.02-0.001）. The findings from reliability analysis for NO, VC, VE, β-CAR, SOD, CAT, GPX, and MDA values used to reflect increased oxidative stress and potential free radical damage in women with PIH showed that the reliability coefficients （alpha, 8 items） = 0.7062, P〈 0.0001, and the standardized item alpha = 0.9116, P〈 0.0001. Conclusion The findings in the present research suggest that pregnancy-induced hypertension can increase oxidative stress and potential free radical damage in women with pregnancy-induced hypertension.展开更多
目的：运用基因芯片技术研究针刺对应激性高血压前期大鼠心脏基因表达的影响。方法：9周龄雄性Wistar大鼠27只,随机分为空白组、模型组、针刺组,每组9只。造模方法：噪声结合足底电击刺激,造模周期为14天。造模同时,针刺组针刺曲池穴、...目的：运用基因芯片技术研究针刺对应激性高血压前期大鼠心脏基因表达的影响。方法：9周龄雄性Wistar大鼠27只,随机分为空白组、模型组、针刺组,每组9只。造模方法：噪声结合足底电击刺激,造模周期为14天。造模同时,针刺组针刺曲池穴、太冲穴进行干预,模型组和空白组每天只做与针刺组相同的抓取刺激。记录在造模前1天、第3、5、7、9、11、13、15天大鼠的血压变化,并观察行为改变。15天后取材,进行基因芯片分析。结果：造模开始后,空白组血压一直正常;第3天,模型组血压开始上升〉120 mm Hg,至第14天,血压持续升高并保持在前期高血压（120~139 mm Hg）状态（P〈0.01）,出现行为改变,提示造模成功。针刺干预第5天开始,针刺组血压低于模型组（P〈0.05）。第15天,模型组已达到高血压水平,针刺组血压虽然没有降至正常,但与模型组相比较差异有统计学意义（P〈0.05）。芯片结果显示：模型组和空白组比较,186个基因表达上调,135个基因表达下调;针刺组和模型组比较,138个基因表达上调,183个基因表达下调;钙卫蛋白（S100A8/A9）、硫氧还蛋白相互作用蛋白（TXNIP）随血压变化,与血压及靶器官损伤密切相关。结论：针刺太冲、曲池穴对于应激性高血压前期大鼠具有明显的降压效应,其机制可能与调控高血压相关基因TXNIP、S100A8/A9的表达有关。展开更多
文摘Objective To investigate whether pregnancy-induced hypertension （PIH） may increase oxidative stress in women with PIH, and to explore the mechanisms by which PIH may increase oxidative stress and potential free radical damage. Methods Seventy women with PIH and seventy women with uncomplicated normotensive pregnancy （UNP） whose age, nutritional conditions, levels of hemoglobin and albumin were all matched, were enrolled in a randomized controlled trial. Their plasma concentrations of nitric oxide （NO）, vitamin C （VC）, vitamin E （VE）, and β-carotene （β-CAR） as well as their erythrocyte malondialdehyde （MDA）, and activities of superoxide dismutase （SOD）, catalase （CAT） and glutathione peroxidase （GPX） were determined by spcctrophotometry. Results Compared with average values of the above experimental parameters in the women with UNP, the average value of erythrocyte MDA in the women with PIH significantly increased （P〈0.0001）, while the average values of plasma NO, VC, VE, and β-CAR as well as those of erythrocyte SOD, CAT, and GPX in the women with PIH significantly decreased （P〈0.0005-0.0001）. The findings from partial correlation analysis （controlling for age） for 70 women with PIH showed that with elevated systolic blood pressure （SBP） and diastolic blood pressure （DBP）, MDA value gradually increased （P〈0.001）, and NO, VC, VE, β-CAR, SOD, CAT, and GPX values gradually decreased （P〈0.02-0.001）. The findings from reliability analysis for NO, VC, VE, β-CAR, SOD, CAT, GPX, and MDA values used to reflect increased oxidative stress and potential free radical damage in women with PIH showed that the reliability coefficients （alpha, 8 items） = 0.7062, P〈 0.0001, and the standardized item alpha = 0.9116, P〈 0.0001. Conclusion The findings in the present research suggest that pregnancy-induced hypertension can increase oxidative stress and potential free radical damage in women with pregnancy-induced hypertension.
文摘目的：运用基因芯片技术研究针刺对应激性高血压前期大鼠心脏基因表达的影响。方法：9周龄雄性Wistar大鼠27只,随机分为空白组、模型组、针刺组,每组9只。造模方法：噪声结合足底电击刺激,造模周期为14天。造模同时,针刺组针刺曲池穴、太冲穴进行干预,模型组和空白组每天只做与针刺组相同的抓取刺激。记录在造模前1天、第3、5、7、9、11、13、15天大鼠的血压变化,并观察行为改变。15天后取材,进行基因芯片分析。结果：造模开始后,空白组血压一直正常;第3天,模型组血压开始上升〉120 mm Hg,至第14天,血压持续升高并保持在前期高血压（120~139 mm Hg）状态（P〈0.01）,出现行为改变,提示造模成功。针刺干预第5天开始,针刺组血压低于模型组（P〈0.05）。第15天,模型组已达到高血压水平,针刺组血压虽然没有降至正常,但与模型组相比较差异有统计学意义（P〈0.05）。芯片结果显示：模型组和空白组比较,186个基因表达上调,135个基因表达下调;针刺组和模型组比较,138个基因表达上调,183个基因表达下调;钙卫蛋白（S100A8/A9）、硫氧还蛋白相互作用蛋白（TXNIP）随血压变化,与血压及靶器官损伤密切相关。结论：针刺太冲、曲池穴对于应激性高血压前期大鼠具有明显的降压效应,其机制可能与调控高血压相关基因TXNIP、S100A8/A9的表达有关。
