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Developing a duplex ARMS-qPCR method to differentiate genotype Ⅰ and Ⅱ African swine fever viruses based on their B646L genes 被引量:2
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作者 DING Lei-lei REN Tao +6 位作者 HUANG Lian-yu Weldu TESFAGABER ZHU Yuan-mao LI Fang SUN En-cheng BU Zhi-gao ZHAO Dong-ming 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2023年第5期1603-1607,共5页
Africanswinefever(ASF),causedbythe African swine fever virus (ASFV), is an acute, hemorrhagic, and contagious disease of domestic pigs and wild boars.The disease is notifiable and listed by the World Organization for ... Africanswinefever(ASF),causedbythe African swine fever virus (ASFV), is an acute, hemorrhagic, and contagious disease of domestic pigs and wild boars.The disease is notifiable and listed by the World Organization for Animal Health (WOAH)(Wang N et al. 2019). 展开更多
关键词 SWINE FEVER duplex
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Joint Flexible Duplexing and Power Allocation with Deep Reinforcement Learning in Cell-Free Massive MIMO System 被引量:4
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作者 Danhao Deng Chaowei Wang +2 位作者 Zhi Zhang Lihua Li Weidong Wang 《China Communications》 SCIE CSCD 2023年第4期73-85,共13页
Network-assisted full duplex(NAFD)cellfree(CF)massive MIMO has drawn increasing attention in 6G evolvement.In this paper,we build an NAFD CF system in which the users and access points(APs)can flexibly select their du... Network-assisted full duplex(NAFD)cellfree(CF)massive MIMO has drawn increasing attention in 6G evolvement.In this paper,we build an NAFD CF system in which the users and access points(APs)can flexibly select their duplex modes to increase the link spectral efficiency.Then we formulate a joint flexible duplexing and power allocation problem to balance the user fairness and system spectral efficiency.We further transform the problem into a probability optimization to accommodate the shortterm communications.In contrast with the instant performance optimization,the probability optimization belongs to a sequential decision making problem,and thus we reformulate it as a Markov Decision Process(MDP).We utilizes deep reinforcement learning(DRL)algorithm to search the solution from a large state-action space,and propose an asynchronous advantage actor-critic(A3C)-based scheme to reduce the chance of converging to the suboptimal policy.Simulation results demonstrate that the A3C-based scheme is superior to the baseline schemes in term of the complexity,accumulated log spectral efficiency,and stability. 展开更多
关键词 cell-free massive MIMO flexible duplexing sum fair spectral efficiency deep reinforcement learning asynchronous advantage actor-critic
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Formation and evolution of the strike-slip faults in the central Sichuan Basin, SW China 被引量:2
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作者 MA Bingshan LIANG Han +7 位作者 WU Guanghui TANG Qingsong TIAN Weizhen ZHANG Chen YANG Shuai ZHONG Yuan ZHANG Xuan ZHANG Zili 《Petroleum Exploration and Development》 SCIE 2023年第2期373-387,共15页
Based on 3D seismic and drilling data, the timing, evolution and genetic mechanism of deep strike-slip faults in the central Sichuan Basin are thoroughly examined by using the U-Pb dating of fault-filled carbonate cem... Based on 3D seismic and drilling data, the timing, evolution and genetic mechanism of deep strike-slip faults in the central Sichuan Basin are thoroughly examined by using the U-Pb dating of fault-filled carbonate cement and seismic-geological analysis. The strike-slip fault system was initially formed in the Late Sinian, basically finalized in the Early Cambrian with dextral transtensional structure, was overlaid with at least one stage of transpressional deformation before the Permian, then was reversed into a sinistral weak transtensional structure in the Late Permian. Only a few of these faults were selectively activated in the Indosinian and later periods. The strike-slip fault system was affected by the preexisting structures such as Nanhuanian rifting normal faults and NW-striking deep basement faults. It is an oblique accommodated intracratonic transfer fault system developed from the Late Sinian to Early Cambrian to adjust the uneven extension of the Anyue trough from north to south and matches the Anyue trough in evolution time and intensity. In the later stage, multiple inversion tectonics and selective activation occurred under different tectonic backgrounds. 展开更多
关键词 strike-slip fault fault timing fault evolution seismic-geological analysis U-Pb dating faulting mechanism cra-tonic Sichuan Basin
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Control of strike-slip faults on Sinian carbonate reservoirs in Anyue gas field, Sichuan Basin, SW China
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作者 HE Xiao TANG Qingsong +5 位作者 WU Guanghui LI Fei TIAN Weizhen LUO Wenjun MA Bingshan SU Chen 《Petroleum Exploration and Development》 SCIE 2023年第6期1282-1294,共13页
The largest Precambrian gas field (Anyue gas field) in China has been discovered in the central Sichuan Basin. However, the deep ancient Ediacaran (Sinian) dolomite presents a substantial challenge due to their tightn... The largest Precambrian gas field (Anyue gas field) in China has been discovered in the central Sichuan Basin. However, the deep ancient Ediacaran (Sinian) dolomite presents a substantial challenge due to their tightness and heterogeneity, rather than assumed large-area stratified reservoirs controlled by mound-shoal microfacies. This complicates the characterization of “sweet spot” reservoirs crucial for efficient gas exploitation. By analyzing compiled geological, geophysical and production data, this study investigates the impact of strike-slip fault on the development and distribution of high-quality “sweet spot” (fractured-vuggy) reservoirs in the Ediacaran dolomite of the Anyue gas field. The dolomite matrix reservoir exhibits low porosity (less than 4%) and low permeability (less than 0.5×10^(-3) μm^(2)). Contrarily, fractures and their dissolution processes along strike-slip fault zone significantly enhance matrix permeability by more than one order of magnitude and matrix porosity by more than one time. Widespread “sweet spot” fracture-vuggy reservoirs are found along the strike-slip fault zone, formed at the end of the Ediacaran. These fractured reservoirs are controlled by the coupling mechanisms of sedimentary microfacies, fracturing and karstification. Karstification prevails at the platform margin, while both fracturing and karstification control high-quality reservoirs in the intraplatform, resulting in reservoir diversity in terms of scale, assemblage and type. The architecture of the strike-slip fault zone governed the differential distribution of fracture zones and the fault-controlled “sweet spot” reservoirs, leading to wide fractured-vuggy reservoirs across the strike-slip fault zone. In conclusion, the intracratonic weak strike-slip fault can play a crucial role in improving tight carbonate reservoir, and the strike-slip fault-related “sweet spot” reservoir emerges as a unique and promising target for the efficient development of deep hydrocarbon resources. Tailored development strategies need to be implemented for these reservoirs, considering the diverse and differential impacts exerted by strike-slip faults on the reservoirs. 展开更多
关键词 pre-Cambrian strike-slip fault carbonate reservoir FRACTURING controlling factor Sichuan Basin
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Investigation on influence of alloying on phase transitions of duplex stainless steel based on thermochemical calculation
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作者 张志强 刘博 +3 位作者 徐连勇 韩永典 赵雷 曲思成 《China Welding》 CAS 2023年第4期11-28,共18页
This paper investigated on influence of different alloying elements added into duplex stainless steel (DSS) on phase transitions using thermochemical methods in comparison with experiment.The results showed that the m... This paper investigated on influence of different alloying elements added into duplex stainless steel (DSS) on phase transitions using thermochemical methods in comparison with experiment.The results showed that the most possible species in the ferrite phase,austenite phase,σphase,Hcp phase,χphase,and carbide were Cr:Va-type,Fe:Va-type,Ni:Cr:Mo-type,Cr_(2)N-type,Fe_(24)Mo_(10)Cr_(24)-type,and Cr:Mo:C-type,respectively.Furthermore,the Ni,N,Cr,and Mo alloying had significant influences on the transition of each DSS phase.The Ni and N additions obviously raised the temperature at ferrite-1/austenite-1 balance while the Cr and Mo decreased the dual-phase balance temperature.In addition,the Ni addition can promote the precipitating ofσphase at relatively high temperature while the precipitating of Hcp phase at relatively low temperature.The Hcp phase andχphase can be obviously increased by the N addition.The introduction of Cr and Mo notably enhances the precipitation ofσphase.However,the promotion ofχphase precipitation is facilitated by the presence of Mo,while the Cr element acts as an inhibitor forχphase precipitation.Furthermore,the ferrite/austenite ratio tested by experiment was higher than that calculated by thermochemical methods,thus pre-designed solution temperature should be lower about 30-100℃than that calculated by thermochemical methods. 展开更多
关键词 phase transitions PRECIPITATION ALLOYING THERMOCHEMISTRY duplex stainless steel
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High throughput screening of localised and general corrosion in type 2205 duplex stainless steel at ambient temperature
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作者 Yiqi Zhou Sultan Mahmood Dirk Lars Engelberg 《International Journal of Minerals,Metallurgy and Materials》 SCIE EI CAS CSCD 2023年第12期2375-2385,共11页
Bipolar electrochemistry is used to produce a linear potential gradient across a bipolar electrode(BPE),providing direct access to the anodic and cathodic reactions under a wide range of applied potentials.The occurre... Bipolar electrochemistry is used to produce a linear potential gradient across a bipolar electrode(BPE),providing direct access to the anodic and cathodic reactions under a wide range of applied potentials.The occurrence of pitting corrosion,crevice corrosion,and general corrosion on type 2205 duplex stainless steel(DSS 2205)BPE has been observed at room temperature.The critical pit depth of 10-20μm with a55%-75% probability of pits developing into stable pits at potential from+0.9 to+1.2 V vs.OCP(open circuit potential)are measured.All pit nucleation sites are either within ferritic grains or at the interface between austenite and ferrite.The critical conditions for pitting and crevice corrosion are discussed with Epit(critical pitting potential)and Ecre(critical crevice potential)decreasing from 0.87 and 0.80 V vs.OCP after150 s of exposure to 0.84 and 0.76 V vs.OCP after 900 s of exposure,respectively.Pit growth kinetics under different applied bipolar potentials and exposure times have been obtained.The ferrite is shown to be more susceptible to general dissolution. 展开更多
关键词 bipolar electrochemistry duplex stainless steel pitting corrosion pit growth factor crevice corrosion
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Literature Review of Phase Transformations and Cavitation Erosion of Duplex Stainless Steels
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作者 Wenji Ai Shanshui Zheng +1 位作者 Xianfeng Zeng Huibing Cheng 《Journal of Materials Science and Chemical Engineering》 2023年第12期10-21,共12页
Phase transformation is one of the factors that would significantly influence the ability to resist cavitation erosion of stainless steels. Due to the specific properties of duplex stainless steel, the heat treatment ... Phase transformation is one of the factors that would significantly influence the ability to resist cavitation erosion of stainless steels. Due to the specific properties of duplex stainless steel, the heat treatment would bring about significant phase transformations. In this paper, we have examined the previous studies on the phase transition of stainless steel, including the literature on the classification of stainless steel, spinodal decomposition, sigma phase transformation, and cavitation erosion of double stainless steel. Through these literature investigations, the destruction of cavitation erosion on duplex stainless steel can be clearly known, and the causes of failure of duplex stainless steel in seawater can be clarified, thus providing a theoretical basis for subsequent scientific research. And the review is about to help assess the possibility of using bulk heat treatment to improve the cavitation erosion (CE) behaviour of the duplex stainless steel 7MoPLUS. 展开更多
关键词 duplex Stainless Steel Phase Transformation Cavitaion Erosion
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Deformation Mechanism of Bimodal Structured 2205 Duplex Stainless Steel in Two Yield Stages
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作者 盛捷 DU Mingchen +7 位作者 LI Yufeng MA Guocai CHEN Weiqian ZHENG Yuehong ZHAN Faqi REN Junqiang G I Raab 喇培清 《Journal of Wuhan University of Technology(Materials Science)》 SCIE EI CAS CSCD 2023年第1期184-191,共8页
A kind of micro/nanostructured 2205 duplex stainless steel(DSS)with uniform distribution of nanocrystals was prepared via aluminothermic reaction method.The analysis of stress-strain curve showed that the fracture str... A kind of micro/nanostructured 2205 duplex stainless steel(DSS)with uniform distribution of nanocrystals was prepared via aluminothermic reaction method.The analysis of stress-strain curve showed that the fracture strength and elongation of the specimen were 946 MPa and 24.7%,respectively.At present,the research on microstructure of bimodal 2205 DSS at room temperature(RT)mainly depended on scanning electron microscope(SEM)observation after loading experiments.The test result indicates that there are two different yield stages in stress-strain curve of specimen during tensile process.The microstructure of duplex bimodal structured stainless steel consists of two pairs of soft hard regions and phases.By studying deformation mechanism of bimodal structured stainless steel,the interaction between soft phase and hard phase are discussed.The principle of composition design and microstructure control of typical duplex stainless steel is obtained,which provides an important research basis for designing of advanced duplex stainless steel. 展开更多
关键词 2205 duplex stainless steel bimodal structure in-situ tensile test deformation mechanism two different yield stages
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In Band Full Duplex (IBFD) Technology for Next Generation Wireless Networks: A Survey in Cellular Networks
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作者 Injila G.R.Begh 《China Communications》 SCIE CSCD 2023年第5期20-39,共20页
Next-Generation(NextG)wireless communication networks with their widespread applications require high data rates,seamless connectivity and high quality of service(QoS).To cope up with an unprecedented rise of data hun... Next-Generation(NextG)wireless communication networks with their widespread applications require high data rates,seamless connectivity and high quality of service(QoS).To cope up with an unprecedented rise of data hungry applications,users demand more spectral resources imposing a limitation on available wireless spectrum.One of the potential solutions to address the spectrum scarce issue is to incorporate in band full duplex(IBFD)or full duplex(FD)paradigm in next generation networks including 5G new radio(NR).Recently,FD has gained the research interest in cellular networks for its potential to double the wireless link capacity and enhancing spectral efficiency(SE).In half duplex(HD)cellular networks,base stations(BSs)can either perform uplink(UL)or downlink(DL)transmission at a particular time instant leading to reduced throughput levels.Due to the advancement in the self interference reduction(SIR)techniques,full duplex base stations(FD-BSs)can be employed to allow simultaneous UL and DL transmissions at the same time–frequency resources as compared to its HD counterpart.It ideally achieves twice the throughput without any additional complexity at user-equipment(UE).This paper covers a detailed survey on FD cellular networks.A series of SIR approaches,UE-UE mitigation techniques are summarized.Various existing MAC protocols and antenna architectures for FD cellular networks are outlined.An overview of security aspects for FD in cellular networks is also presented.Lastly,various open issues and possible research directions are brought up for FD cellular networks. 展开更多
关键词 cellular network full duplex(FD)communication self interference reduction(SIR) three node full-duplex(TNFD)topology UE-UE interference
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鉴别猪圆环病毒2型和3型双重TaqMan MGB探针FQ-PCR检测方法研究
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作者 赵雪丽 闫若潜 +8 位作者 王华俊 王淑娟 马震原 谢彩华 柴茂 杨海波 王翠 刘影 王东方 《中国动物传染病学报》 CAS 北大核心 2024年第2期165-173,共9页
建立一种快速、特异鉴别检测猪圆环病毒2型(PCV2)和猪圆环病毒3型(PCV3)的双重TaqMan MGB探针FQ-PCR方法,本研究以PCV2的Rep蛋白和PCV3的Cap蛋白基因作为靶基因,各设计1对特异性引物和1条TaqMan MGB探针,经优化各反应条件和进行敏感性... 建立一种快速、特异鉴别检测猪圆环病毒2型(PCV2)和猪圆环病毒3型(PCV3)的双重TaqMan MGB探针FQ-PCR方法,本研究以PCV2的Rep蛋白和PCV3的Cap蛋白基因作为靶基因,各设计1对特异性引物和1条TaqMan MGB探针,经优化各反应条件和进行敏感性、特异性、重复性和干扰性试验,建立鉴别检测PCV2/PCV3的双重FQ-PCR方法。结果显示:该方法可特异性扩增PCV2、PCV3核酸,与猪伪狂犬病病毒(PRV)等8种病原及阴性对照无交叉反应,特异性较强;对PCV2和PCV3阳性质粒标准品的最低检出限均可达10 copies/μL,敏感性较高;PCV2/PCV3批内/批间重复试验变异系数(CV)值均在3%以下,表明方法稳定性、重复性较好;干扰性试验表明在两种病毒阳性质粒起始模板相差较大时该方法不会影响对其中任一病毒核酸的检出和准确定量。对42份临床疑似PCV感染样品检测结果与PCV2、PCV3基因测序结果符合率100%。本研究建立的双重FQ-PCR方法具有敏感性高达10 copies/μL、特异性强、在同一反应体系中能同时快速鉴别检测PCV2、PCV3等优点,可用于临床PCV2/PCV3感染的快速鉴别检测。 展开更多
关键词 猪圆环病毒2型 rep基因 猪圆环病毒3型 cap基因 双重TaqMan MGB FQ-PCR
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水下超双部件氢致开裂数值评估与实验
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作者 曹永 姜瑛 +1 位作者 徐祥娟 钱思成 《海洋工程装备与技术》 2024年第1期49-53,共5页
水下生产系统在我国海洋石油开发中应用越来越广泛。通常使用复合电液的方式对水下设备进行远程控制,控制管线采用超级双相不锈钢材质。但该材质在水下阴极保护系统下存在氢致开裂的风险。本文依据国外规范,建立了水下超双材料的氢致开... 水下生产系统在我国海洋石油开发中应用越来越广泛。通常使用复合电液的方式对水下设备进行远程控制,控制管线采用超级双相不锈钢材质。但该材质在水下阴极保护系统下存在氢致开裂的风险。本文依据国外规范,建立了水下超双材料的氢致开裂评估流程,详细阐述了水下超双部件氢致开裂的有限元评估过程,给出了建模方法、结果提取和处理的方法,以及结果校核的标准;结合实际工程项目实例计算分析,将分析结果线性化处理;控制部件的应力应变水平,可以有效避免部件在水下同阴极保护系统作用下的氢致开裂风险;研究和阐述了双相钢的适用环境参数条件和氢致开裂试验评估。 展开更多
关键词 氢致开裂 超双部件 HISC
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无人机辅助蜂窝网络的模式选择与资源优化
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作者 冯大权 郑灿健 孔祥琦 《物联网学报》 2024年第1期29-39,共11页
研究了无人机(UAV,unmanned aerial vehicle)与蜂窝网络共存下的无线通信资源分配和优化方案。为了提高网络频谱利用率,无人机用户以全双工(FD,full duplex)或半双工(HD,half duplex)设备对设备(D2D,device-to-device)技术复用蜂窝频谱... 研究了无人机(UAV,unmanned aerial vehicle)与蜂窝网络共存下的无线通信资源分配和优化方案。为了提高网络频谱利用率,无人机用户以全双工(FD,full duplex)或半双工(HD,half duplex)设备对设备(D2D,device-to-device)技术复用蜂窝频谱资源接入网络。此外,构造了一个联合接入控制、模式选择、功率控制和资源分配优化问题最大化网络的整体吞吐量,并保证无人机用户和地面蜂窝用户的服务质量要求。为解决这个问题,首先利用凸优化的第一阶段方法分别对全双工和半双工两种设备对设备模式进行接入控制和可行性判定。然后,对可接入无人机用户对使用凸凹过程(CCCP,convex and concave procedure)迭代算法求解功率控制问题。利用该局部最优值,原优化问题可以简化为加权最大化问题。最后,通过库恩-芒克斯(KM,Kuhn-Munrkes)算法对最优信道资源进行匹配,获得系统的全局最优吞吐量值。数值结果表明,所提方案能显著提高系统性能。 展开更多
关键词 无人机通信 全双工设备对设备技术 模式选择 功率控制 资源分配与优化
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床旁彩色及脉冲多普勒(双功能)超声评估原位肝移植术后并发症
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作者 黄媛 王志辉 +1 位作者 刘娅妮 崔新伍 《中国医学影像技术》 CSCD 北大核心 2024年第3期383-386,共4页
目的观察床旁彩色及脉冲多普勒(双功能)超声评估原位肝移植(OLT)术后并发症的价值。方法回顾性分析65例接受OLT患者术后第1、3、7天床旁腹部双功能超声检查资料,评估肝脏实质回声及肝固有动脉及门静脉等血流束,测量门静脉峰值流速(PVPV... 目的观察床旁彩色及脉冲多普勒(双功能)超声评估原位肝移植(OLT)术后并发症的价值。方法回顾性分析65例接受OLT患者术后第1、3、7天床旁腹部双功能超声检查资料,评估肝脏实质回声及肝固有动脉及门静脉等血流束,测量门静脉峰值流速(PVPV)、肝动脉主干收缩期血流峰值流速(PSV)及血流阻力指数(RI)。根据腹部CT增强、CT血管成像(CTA)、超声造影(CEUS)、临床干预(包括增强免疫抑制剂和二次移植手术)或病理结果,绘制受试者工作特征(ROC)曲线,计算曲线下面积(AUC),评估以OLT后第7天内超声所测PSV(PSV_(第7天))及RI(RI_(第7天))判断有无并发症的效能。结果65例OLT后第1、3及7天,PVPV分别为71.00(45.50,96.50)、57.50(42.00,71.00)及50.00(33.00,66.00)cm/s,RI分别为0.68±0.16、0.69±0.17、0.66±0.13,各时间点间PVPV及RI差异均无统计学意义(H=5.475、P=0.065,F=0.964、P=0.501);PSV分别为63.00(44.50,89.00)、47.00(31.50,64.50)及50.00(38.25,64.75)cm/s,各时间点差异有统计学意义(H=7.313,P=0.026)。65例中,PSV正常46例、偏低(PSV<25 cm/s)1例、偏高(PSV>100 cm/s)18例;RI正常18例、偏低(RI<0.5)11例、偏高(RI>0.7)36例。36例出现OLT后并发症。以PSV_(第7天)诊断OLT后并发症的AUC为0.561,RI_(第7天)为0.699,PSV_(第7天)联合RI_(第7天)为0.732,与单独RI_(第7天)差异无统计学意义(Z=0.290,P>0.05)。结论利用床旁双功能超声监测移植肝动脉主干血流信号及RI可有效评估OLT后并发症。 展开更多
关键词 肝移植 超声检查 多普勒 双功能 手术后并发症
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猫冠状病毒和猫细小病毒双重荧光定量PCR方法的建立
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作者 郭存杰 王蕾 +3 位作者 毕振威 钱晶 张传美 谭业平 《中国动物检疫》 CAS 2024年第4期91-95,共5页
为建立猫冠状病毒(feline coronavirus,FCoV)和猫细小病毒(feline parvovirus,FPV)的双重荧光定量PCR方法,选取FCoV 3'UTR和FPV VP2基因保守区域,分别设计2对特异性引物和TaqMan MGB探针,并进行反应体系和条件优化,以及特异性、敏... 为建立猫冠状病毒(feline coronavirus,FCoV)和猫细小病毒(feline parvovirus,FPV)的双重荧光定量PCR方法,选取FCoV 3'UTR和FPV VP2基因保守区域,分别设计2对特异性引物和TaqMan MGB探针,并进行反应体系和条件优化,以及特异性、敏感性和重复性试验,探讨所建方法的可行性。结果显示:该方法可特异性检出FCoV和FPV,与猫疱疹病毒、猫杯状病毒、猫轮状病毒、支原体、衣原体、波氏杆菌、犬腺病毒和犬副流感病毒等病原核酸无交叉反应,对FCoV和FPV检测限均为1 copies/μL;FCoV和FPV阳性参考质粒组间和组内重复试验变异系数均小于3%;对35份临床样本进行检测,发现建立的双重荧光定量PCR方法阳性检出率比普通PCR方法高。结果表明,本研究建立的双重荧光定量PCR方法具有灵敏、特异和稳定等优点,可用于临床FCoV和FPV感染的早期鉴别诊断。 展开更多
关键词 猫冠状病毒 猫细小病毒 双重荧光定量PCR
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Duplex PCR快速检测松材线虫 被引量:4
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作者 赵立荣 钟国强 廖金铃 《植物检疫》 北大核心 2004年第6期324-326,共3页
利用duplexPCR技术对松材线虫 (Bursaphelenchusxylophilus)与拟松材线虫 (B .mu cronatus)的rDNA部分核苷酸序列扩增。根据松材线虫与拟松材线虫的ITS1序列区别 ,设计出特异性引物 ,检测松材线虫的存在 ;在 5 8S ,2 8S保守序列区设计... 利用duplexPCR技术对松材线虫 (Bursaphelenchusxylophilus)与拟松材线虫 (B .mu cronatus)的rDNA部分核苷酸序列扩增。根据松材线虫与拟松材线虫的ITS1序列区别 ,设计出特异性引物 ,检测松材线虫的存在 ;在 5 8S ,2 8S保守序列区设计通用引物 。 展开更多
关键词 拟松材线虫 PCR快速检测 保守序列 特异性引物 通用引物 核苷酸序列 利用 扩增 PCR技术 DNA
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表面织构-活性屏离子氮化复合处理对38CrMoAl钢摩擦学行为的影响
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作者 王杰 陈伟敬 +6 位作者 林乃明 王强 王焕焕 袁烁 刘志奇 曾群锋 吴玉程 《太原理工大学学报》 CAS 北大核心 2024年第3期483-492,共10页
【目的】38CrMoAl钢是液压泵柱塞常用材质之一,其表面性能对液压泵长寿命安全服役尤为重要。磨损是液压泵柱塞的主要失效方式,采用表面强化处理能够突破其性能极限,是实现液压泵高性能、高可靠运行的有效途径。【方法】采用表面织构化... 【目的】38CrMoAl钢是液压泵柱塞常用材质之一,其表面性能对液压泵长寿命安全服役尤为重要。磨损是液压泵柱塞的主要失效方式,采用表面强化处理能够突破其性能极限,是实现液压泵高性能、高可靠运行的有效途径。【方法】采用表面织构化和活性屏离子氮化的复合处理工艺对38CrMoAl钢进行表面形性调控,以期实现其表面强化方法。先利用激光加工技术在38CrMoAl钢表面制备凹坑织构,再对38CrMoAl钢和表面织构化38CrMoAl钢进行活性屏离子氮化处理。借助球-盘式摩擦磨损试验机研究表面织构-活性屏离子氮化复合处理对38CrMoAl钢摩擦学行为的影响。【结果】结果表明,38CrMoAl钢表面生成了ε-Fe2-3N、γ'-Fe4N和CrN;氮化层表面致密且无明显裂纹和孔洞;氮化层均匀、连续,由白亮层和扩散层组成。氮化层的截面硬度呈梯度分布,试样表面硬度最高,随着距表面距离的增大,硬度逐渐降低。38CrMoAl钢相关试样与Al_(2)O_(3)球的干滑动摩擦结果可知,38CrMoAl钢的摩擦系数为0.40,而表面织构-活性屏离子氮化38CrMoAl钢的摩擦系数为0.36;经复合处理后,38CrMoAl钢的磨损失重降低了92.5%.复合处理显著提高了38CrMoAl钢的摩擦学性能。 展开更多
关键词 表面织构 活性屏离子氮化 复合处理 38CRMOAL钢 摩擦学性能
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非洲猪瘟病毒、高致病性猪繁殖与呼吸综合征病毒二重实时荧光定量PCR检测方法的建立与初步应用
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作者 刘影 闫若潜 +10 位作者 王东方 杨海波 赵美雪 宋丹 赵雪丽 谢彩华 王淑娟 马震原 柴茂 王翠 刘梅芬 《中国动物传染病学报》 CAS 北大核心 2024年第2期118-130,共13页
建立一种特异、敏感、快速的实时荧光定量PCR(FQ-PCR)方法,用于非洲猪瘟病毒(ASFV)和高致病性猪繁殖与呼吸综合征病毒(HP-PRRSV)的鉴别诊断。针对ASFV的B646L基因和HP-PRRSV的NSP2基因分别设计特异性引物/探针对,经优化反应体系、反应... 建立一种特异、敏感、快速的实时荧光定量PCR(FQ-PCR)方法,用于非洲猪瘟病毒(ASFV)和高致病性猪繁殖与呼吸综合征病毒(HP-PRRSV)的鉴别诊断。针对ASFV的B646L基因和HP-PRRSV的NSP2基因分别设计特异性引物/探针对,经优化反应体系、反应程序等反应条件,建立一种基于探针技术的FQ-PCR方法,验证方法的敏感性、特异性和重复性,对130份临床样品进行检测,并与OIE检测方法(ASFV)及国标方法(HP-PRRSV)进行比较分析。本研究成功建立的ASFV和HP-PRRSV二重FQ-PCR检测方法在10^(-1)~10^(5) copies/μL模板范围内有良好的线性关系;对ASFV和HP-PRRSV基因出现阳性扩增,但对猪日本乙型脑炎病毒(JEV)、猪瘟(CSFV)、猪细小病毒(PPV)、猪伪狂犬病病毒(PRV)、猪圆环病毒2型(PCV2)、猪繁殖与呼吸综合征病毒(PRRSV)美洲经典株(VR2332株)、健康猪脾脏等7种病原核酸样品对照未出现扩增;批内、批间试验变异系数在0.53%~3.14%,重复性良好;对ASFV和HP-PRRSV的最低检测模板浓度均为10 copies/μL;利用建立的二重FQ-PCR方法对130份临床样品进行检测,检测结果与OIE检测方法(ASFV)及国标方法(HP-PRRSV)完全一致。本研究成功建立了鉴别ASFV和HPPRRSV二重FQ-PCR检测方法,为ASFV和HP-PRRSV的鉴别诊断提供了快速、敏感、特异且能满足临床检测需求的检测方法。 展开更多
关键词 非洲猪瘟病毒 高致病性猪繁殖与呼吸综合征病毒 二重FQ-PCR
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海洋平台换热器橇海水系统超级双相钢耐蚀分析
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作者 刘羽 吴旭维 +2 位作者 于本水 郭海 张柏青 《科技和产业》 2024年第9期266-270,共5页
集合国外行业标准和国内使用经验,对海洋石油平台管壳式换热器橇内海水系统超级双相钢管线频繁泄漏进行研究,探析冶金状态、海水温度、焊接特性、材料污染为超级双相钢在海水系统中腐蚀泄漏的主要原因。从系统工艺、冶金、焊接、运行等... 集合国外行业标准和国内使用经验,对海洋石油平台管壳式换热器橇内海水系统超级双相钢管线频繁泄漏进行研究,探析冶金状态、海水温度、焊接特性、材料污染为超级双相钢在海水系统中腐蚀泄漏的主要原因。从系统工艺、冶金、焊接、运行等方面分别提出减少泄漏风险的方法,并在后续设备改造中得到良好的应用。 展开更多
关键词 管壳式换热器 海水系统 超级双相钢 点蚀
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时效对超级双相不锈钢析出相演变的影响
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作者 骆仁智 胡文豪 +3 位作者 王福明 涂江平 陶应啟 沈伟 《特殊钢》 2024年第1期77-81,共5页
通过在850℃时效处理研究了S32750超级双相不锈钢析出相的析出行为。采用Thermo-Calc热力学软件预测析出相及相组成,采用OM,FE-SEM和EDS对S32750超级双相不锈钢铸坯不同时效状态下的样品进行全面分析,利用K-J-M-A模型进行σ相析出动力... 通过在850℃时效处理研究了S32750超级双相不锈钢析出相的析出行为。采用Thermo-Calc热力学软件预测析出相及相组成,采用OM,FE-SEM和EDS对S32750超级双相不锈钢铸坯不同时效状态下的样品进行全面分析,利用K-J-M-A模型进行σ相析出动力学拟合。实验结果表明:850℃时效过程中,σ相优先在铁素体内部和双相晶界处析出,直至铁素体相耗尽;σ相的析出动力学控速环节主要是:初始阶段形核控速和时效40 min后的元素扩散控速;除σ相外,时效过程中还会析出χ相和Cr2N相。其中χ相属于亚稳定相,主要在时效前期析出,随着时效时间的延长最终转化成σ相。 展开更多
关键词 双相不锈钢 析出相 析出动力学 时效
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热效应所致析出相对2205双相不锈钢耐蚀性的影响
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作者 夏荣华 唐建群 巩建鸣 《南京工业大学学报(自然科学版)》 CAS 北大核心 2024年第3期300-309,共10页
研究不同热效应下2205双相不锈钢的析出相类型和特点,通过测试固溶态和不同热效应下2205双相钢的动电位极化曲线、电化学阻抗谱以及动电位扫描后试样表面形貌的观察,研究析出相对其耐腐蚀性能的影响。结果表明:固溶态材料的耐腐蚀性能... 研究不同热效应下2205双相不锈钢的析出相类型和特点,通过测试固溶态和不同热效应下2205双相钢的动电位极化曲线、电化学阻抗谱以及动电位扫描后试样表面形貌的观察,研究析出相对其耐腐蚀性能的影响。结果表明:固溶态材料的耐腐蚀性能及再钝化性能最好;而在850℃时效后,由于χ和σ相析出含量增多,在过电位下会由点蚀转变为选择性腐蚀,导致材料的耐腐蚀性能下降最严重;900℃时效后,2205双相不锈钢中的析出相含量降低,其耐腐蚀性能的下降有所缓解。 展开更多
关键词 2205双相不锈钢 析出相 极化曲线 电化学阻抗谱 耐蚀性
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