Objective:To study the blood coagulation function, degree of inflammation as well as apoptosis in brain tissue of rat models with heat stroke. Methods: Healthy male SD rats were selected as experimental animals and ra...Objective:To study the blood coagulation function, degree of inflammation as well as apoptosis in brain tissue of rat models with heat stroke. Methods: Healthy male SD rats were selected as experimental animals and randomly divided into heat stroke group and control group, heat stroke group were raised under the condition of environment temperature (40.0±0.5)℃and relative humidity 60%to establish heat stroke models, the anus temperature reached 42.5℃, and the control group were conventionally raised;60 and 120 min after model establishment, the blood coagulation function, serum inflammatory factor levels as well as brain water content, the number of apoptotic cells and apoptotic molecule expression were determined respectively. Results: (1) blood biochemical indexes:Activated partial thromboplastin time (APTT) and prothrombin time (PT) of heat stroke group were longer than those of control group, and serum D-Dimer content (D-D), tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β) and IL-6 levels were significantly higher than those of control group (P<0.05);(2) the brain tissue damage indexes:brain water content, the number of apoptotic cells, Caspase-3 expression and LC3-II/LC3-I ratio of heat stroke group were significantly higher than those of control group (P<0.05). Conclusions:There are blood coagulation dysfunction and systemic inflammation activation in heat stroke model rats, and the apoptosis in brain tissue increases obviously.展开更多
Objective:To investigate the effect of Shuxuetong injection on platelet function, hemorheology and cerebral blood flow in patients with ischemic stroke.Methods: One hundred cases of ischemic stroke admitted in our hos...Objective:To investigate the effect of Shuxuetong injection on platelet function, hemorheology and cerebral blood flow in patients with ischemic stroke.Methods: One hundred cases of ischemic stroke admitted in our hospital from February 2015 to January 2017 were randomly divided into control group and observation group. The control group was given routine treatment. The observation group was given Shuxuetong injection on the basis of the control group. The changes of platelet function, hemorheology and cerebral blood flow before and after treatment were detected in both groups.Results: After treatment, the whole blood viscosity, plasma viscosity and fibrinogen level in both groups were significantly lower than those before treatment. The whole blood viscosity, plasma viscosity and fibrinogen level in the observation group were (4.18±0.05) mPa?s, (1.66±0.12) mPa?s and (3.45±0.08) g/L, respectively, which were significantly lower than the control group;After treatment, the levels of MCA, ACA and PCA in both groups were significantly increased. MCA, ACA and PCA levels in the observation group were (70.82±4.13) cm/s, (60.62±3.55) cm/s and (54.11±2.36) cm/s, which were significantly higher than those in the control group;After treatment, the maximum platelet aggregation rate, PLT, MPV and PDW levels in the two groups significantly decreased. The maximum platelet aggregation rate, PLT, MPV and PDW levels in the observation group were (27.93±1.44)% and (155.32±13.46)×109/L, (9.42±0.32) fL and (9.12±0.24) fL, respectively, which were significantly lower than those in the control group. Conclusions: Shuxuetong injection can effectively improve the patient's hemorheology and platelet function, improve the level of cerebral blood flow in patients with significant effect, it is worth further clinical application.展开更多
Background：Bipolar electro-coagulation has a reported efficacy in treating epilepsy involving functional cortex by pure electro-coagulation or combination with resection.However,the mechanisms of bipolar electro-coag...Background：Bipolar electro-coagulation has a reported efficacy in treating epilepsy involving functional cortex by pure electro-coagulation or combination with resection.However,the mechanisms of bipolar electro-coagulation are not completely known.We studied the acute cortical blood flow and histological changes after bipolar electro-coagulation in 24 patients with intractable temporal lobe epilepsy.Methods：Twenty-four patients were consecutively enrolled,and divided into three groups according to the date of admission.The regional cortical blood flow （rCBF）,electrocorticography,the depth of cortex damage,and acute histological changes （H and E staining,neuronal staining and neurofilament （NF） staining） were analyzed before and after the operation.The t-test analysis was used to compare the rCBF before and after the operation.Results：The rCBF after coagulation was significantly reduced （P 〈 0.05）.The spikes were significantly reduced after electro-coagulation.For the temporal cortex,the depth of cortical damage with output power of 2-9 W after electro-coagulation was 0.34 ± 0.03,0.48 ± 0.06,0.69 ± 0.06,0.84 ± 0.09,0.98 ± 0.08,1.10 ± 0.1 l,1.11 ± 0.09,and 1.22 ± 0.11 mm,respectively.Coagulation with output power of 4-5 W completely damaged the neurons and NF protein in the molecular layer,external granular layer,and external pyramidal layer.Conclusions：The electro-coagulation not only destroyed the neurons and NF protein,but also reduced the rCBF.We concluded that the injuries caused by electro-coagulation would prevent horizontal synchronization and spread of epileptic discharges,and partially destroy the epileptic focus.展开更多
目的:对2型糖尿病患者瘀血舌象与PT、INR、APTT、FIB四项凝血指标之间的相关性进行探讨。方法:用Sysm ex CA1500全自动血凝分析仪检测136例2型糖尿病患者,77例正常人群体检者的凝血酶原时间(PT)、活化部分凝血酶时间(APTT)、国际标准化...目的:对2型糖尿病患者瘀血舌象与PT、INR、APTT、FIB四项凝血指标之间的相关性进行探讨。方法:用Sysm ex CA1500全自动血凝分析仪检测136例2型糖尿病患者,77例正常人群体检者的凝血酶原时间(PT)、活化部分凝血酶时间(APTT)、国际标准化比值(INR)、纤维蛋白原(FIB)。结果:瘀血舌象组、非瘀血舌象组的PT均比正常组显著缩短(P<0.017,P<0.001);瘀血舌象组、非瘀血舌象组的APTT均比正常组显著缩短(P<0.001,P<0.017);非瘀血舌象组FIB水平显著高于正常组(P<0.017);瘀血舌象组FIB水平显著高于非瘀血舌象组(P<0.001)。结论:糖尿病患者体内凝血功能增强、纤溶功能升高,而糖尿病血瘀证患者体内凝血功能增强、纤溶功能升高可能是其出现瘀血舌象的病理基础之一。展开更多
文摘Objective:To study the blood coagulation function, degree of inflammation as well as apoptosis in brain tissue of rat models with heat stroke. Methods: Healthy male SD rats were selected as experimental animals and randomly divided into heat stroke group and control group, heat stroke group were raised under the condition of environment temperature (40.0±0.5)℃and relative humidity 60%to establish heat stroke models, the anus temperature reached 42.5℃, and the control group were conventionally raised;60 and 120 min after model establishment, the blood coagulation function, serum inflammatory factor levels as well as brain water content, the number of apoptotic cells and apoptotic molecule expression were determined respectively. Results: (1) blood biochemical indexes:Activated partial thromboplastin time (APTT) and prothrombin time (PT) of heat stroke group were longer than those of control group, and serum D-Dimer content (D-D), tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β) and IL-6 levels were significantly higher than those of control group (P<0.05);(2) the brain tissue damage indexes:brain water content, the number of apoptotic cells, Caspase-3 expression and LC3-II/LC3-I ratio of heat stroke group were significantly higher than those of control group (P<0.05). Conclusions:There are blood coagulation dysfunction and systemic inflammation activation in heat stroke model rats, and the apoptosis in brain tissue increases obviously.
文摘Objective:To investigate the effect of Shuxuetong injection on platelet function, hemorheology and cerebral blood flow in patients with ischemic stroke.Methods: One hundred cases of ischemic stroke admitted in our hospital from February 2015 to January 2017 were randomly divided into control group and observation group. The control group was given routine treatment. The observation group was given Shuxuetong injection on the basis of the control group. The changes of platelet function, hemorheology and cerebral blood flow before and after treatment were detected in both groups.Results: After treatment, the whole blood viscosity, plasma viscosity and fibrinogen level in both groups were significantly lower than those before treatment. The whole blood viscosity, plasma viscosity and fibrinogen level in the observation group were (4.18±0.05) mPa?s, (1.66±0.12) mPa?s and (3.45±0.08) g/L, respectively, which were significantly lower than the control group;After treatment, the levels of MCA, ACA and PCA in both groups were significantly increased. MCA, ACA and PCA levels in the observation group were (70.82±4.13) cm/s, (60.62±3.55) cm/s and (54.11±2.36) cm/s, which were significantly higher than those in the control group;After treatment, the maximum platelet aggregation rate, PLT, MPV and PDW levels in the two groups significantly decreased. The maximum platelet aggregation rate, PLT, MPV and PDW levels in the observation group were (27.93±1.44)% and (155.32±13.46)×109/L, (9.42±0.32) fL and (9.12±0.24) fL, respectively, which were significantly lower than those in the control group. Conclusions: Shuxuetong injection can effectively improve the patient's hemorheology and platelet function, improve the level of cerebral blood flow in patients with significant effect, it is worth further clinical application.
文摘Background：Bipolar electro-coagulation has a reported efficacy in treating epilepsy involving functional cortex by pure electro-coagulation or combination with resection.However,the mechanisms of bipolar electro-coagulation are not completely known.We studied the acute cortical blood flow and histological changes after bipolar electro-coagulation in 24 patients with intractable temporal lobe epilepsy.Methods：Twenty-four patients were consecutively enrolled,and divided into three groups according to the date of admission.The regional cortical blood flow （rCBF）,electrocorticography,the depth of cortex damage,and acute histological changes （H and E staining,neuronal staining and neurofilament （NF） staining） were analyzed before and after the operation.The t-test analysis was used to compare the rCBF before and after the operation.Results：The rCBF after coagulation was significantly reduced （P 〈 0.05）.The spikes were significantly reduced after electro-coagulation.For the temporal cortex,the depth of cortical damage with output power of 2-9 W after electro-coagulation was 0.34 ± 0.03,0.48 ± 0.06,0.69 ± 0.06,0.84 ± 0.09,0.98 ± 0.08,1.10 ± 0.1 l,1.11 ± 0.09,and 1.22 ± 0.11 mm,respectively.Coagulation with output power of 4-5 W completely damaged the neurons and NF protein in the molecular layer,external granular layer,and external pyramidal layer.Conclusions：The electro-coagulation not only destroyed the neurons and NF protein,but also reduced the rCBF.We concluded that the injuries caused by electro-coagulation would prevent horizontal synchronization and spread of epileptic discharges,and partially destroy the epileptic focus.
文摘目的:对2型糖尿病患者瘀血舌象与PT、INR、APTT、FIB四项凝血指标之间的相关性进行探讨。方法:用Sysm ex CA1500全自动血凝分析仪检测136例2型糖尿病患者,77例正常人群体检者的凝血酶原时间(PT)、活化部分凝血酶时间(APTT)、国际标准化比值(INR)、纤维蛋白原(FIB)。结果:瘀血舌象组、非瘀血舌象组的PT均比正常组显著缩短(P<0.017,P<0.001);瘀血舌象组、非瘀血舌象组的APTT均比正常组显著缩短(P<0.001,P<0.017);非瘀血舌象组FIB水平显著高于正常组(P<0.017);瘀血舌象组FIB水平显著高于非瘀血舌象组(P<0.001)。结论:糖尿病患者体内凝血功能增强、纤溶功能升高,而糖尿病血瘀证患者体内凝血功能增强、纤溶功能升高可能是其出现瘀血舌象的病理基础之一。
