In this study area the geological conditions are complicated and the effective sandstone is very heterogeneous.The sandstones are thin and lateral and vertical variations are large.We introduce multi-attribute fusion ...In this study area the geological conditions are complicated and the effective sandstone is very heterogeneous.The sandstones are thin and lateral and vertical variations are large.We introduce multi-attribute fusion technology based on pre-stack seismic data, pre-stack P-and S-wave inversion results,and post-stack attributes.This method not only can keep the fluid information contained in pre-stack seismic data but also make use of the high SNR characteristics of post-stack data.First,we use a one-step recursive method to get the optimal attribute combination from a number of attributes.Second,we use a probabilistic neural network method to train the nonlinear relationship between log curves and seismic attributes and then use the trained samples to find the natural gamma ray distribution in the Su-14 well block and improve the resolution of seismic data.Finally,we predict the effective reservoir distribution in the Su-14 well block.展开更多
Block of proliferation 1(BOP1)is a key protein involved in ribosome maturation and affects cancer progression.However,its role in gastric cancer(GC)remains unknown.This study aimed to explore the expression of BOPI in...Block of proliferation 1(BOP1)is a key protein involved in ribosome maturation and affects cancer progression.However,its role in gastric cancer(GC)remains unknown.This study aimed to explore the expression of BOPI in GC and its potential mechanisms in regulating GC growth,and the relationship between BOP1 level in cancer tissues and survival was also analyzed.The expression of BOPI was examined by immunohistochemistry(IHC)in a cohort containing 387 patients with primary GC.Cultured GC cells were treated by siRNA to knock down the BOP1 expression,and examined by CCK-8 assay and plate clone formation to assess cell proliferation in vitro.Apoptotic rate of cultured GC cells was detected by flow cytometry with double staining of AnnxinV/PI.The xenografted mouse model was used to assess GC cell proliferation in vivo.Western blot and IHC were also performed to detect the expression levels of BOP1,p53 and p21.Patients with higher level of BOP1 in cancer tissues had significantly poorer survival.BOP1 silencing signifcantly suppressed GC cell proliferation both in vitro and in vivo.It blocked cell cycle at G0/G1 phase and led to apoptosis of GC cells via upregulating p53 and p21.BOP1 silencing-induced suppression of cell proliferation was partly reversed by pifithrin-a,(a p53 inhibitor).Our study dermonstrated that BOP1 up-regulation may be a hallmark of GC and it may regulate proliferation of GC cells by activating p53.BOP1 might be considered a novel biomarker of GC proliferation,and could be a potential indicator of prognosis of GC patients.BOP1 might also be a potential target for the treatment of GC patients if further researched.展开更多
基金The National Natural Science Foundation of China and China Petroleum&Chemical Corporation Co-funded Project(Grant Nos 40839905 and 40739907)
文摘In this study area the geological conditions are complicated and the effective sandstone is very heterogeneous.The sandstones are thin and lateral and vertical variations are large.We introduce multi-attribute fusion technology based on pre-stack seismic data, pre-stack P-and S-wave inversion results,and post-stack attributes.This method not only can keep the fluid information contained in pre-stack seismic data but also make use of the high SNR characteristics of post-stack data.First,we use a one-step recursive method to get the optimal attribute combination from a number of attributes.Second,we use a probabilistic neural network method to train the nonlinear relationship between log curves and seismic attributes and then use the trained samples to find the natural gamma ray distribution in the Su-14 well block and improve the resolution of seismic data.Finally,we predict the effective reservoir distribution in the Su-14 well block.
基金supported by National Natural Science Foundation of China(No.31670806)。
文摘Block of proliferation 1(BOP1)is a key protein involved in ribosome maturation and affects cancer progression.However,its role in gastric cancer(GC)remains unknown.This study aimed to explore the expression of BOPI in GC and its potential mechanisms in regulating GC growth,and the relationship between BOP1 level in cancer tissues and survival was also analyzed.The expression of BOPI was examined by immunohistochemistry(IHC)in a cohort containing 387 patients with primary GC.Cultured GC cells were treated by siRNA to knock down the BOP1 expression,and examined by CCK-8 assay and plate clone formation to assess cell proliferation in vitro.Apoptotic rate of cultured GC cells was detected by flow cytometry with double staining of AnnxinV/PI.The xenografted mouse model was used to assess GC cell proliferation in vivo.Western blot and IHC were also performed to detect the expression levels of BOP1,p53 and p21.Patients with higher level of BOP1 in cancer tissues had significantly poorer survival.BOP1 silencing signifcantly suppressed GC cell proliferation both in vitro and in vivo.It blocked cell cycle at G0/G1 phase and led to apoptosis of GC cells via upregulating p53 and p21.BOP1 silencing-induced suppression of cell proliferation was partly reversed by pifithrin-a,(a p53 inhibitor).Our study dermonstrated that BOP1 up-regulation may be a hallmark of GC and it may regulate proliferation of GC cells by activating p53.BOP1 might be considered a novel biomarker of GC proliferation,and could be a potential indicator of prognosis of GC patients.BOP1 might also be a potential target for the treatment of GC patients if further researched.
