AIM To investigate the role of tumor necrosisfactor(TNF)in lung injury during acutenecrotizing pancreatitis(ANP),and thetherapeutic effect of'Tong Xia'purgativemethod in minimizing the severity of lung injury....AIM To investigate the role of tumor necrosisfactor(TNF)in lung injury during acutenecrotizing pancreatitis(ANP),and thetherapeutic effect of'Tong Xia'purgativemethod in minimizing the severity of lung injury.METHODS Fourteen canines were randomlydivided into 3 groups:the'Tong Xia'treatmentgroup(n = 5)using Dachengqitang;salinecontrol group(n = 5),and the sham operationgroup(n = 4).TNF activity in serum and inbronchoalveolar lavage fluid(BALF),the serumendotoxin levels were measured,and theseverity of lung injury evaluated.RESULTS Elevation of TNF activity was moreprominent in BALF than in serum.TNF activity inserum at 6 and 12 hours and in BALI:wassignificantly decreased in the'Tong Xia'treatment group than in the saline control one(q=21.11,q=12.07,q=9.03,respectively,P【0.01)and the lung injury was significantlyalleviated at 12 hours as compared with that inthe saline group,manifested as amelioration otthe lung wet/ dry weight ratio,decrease inprotein concentration and neutrophils count inBALF,and improvement of pulmonaryinflammatory changes.A positive correlationwas demonstrated between serum TNF activity and endotoxin level.CONCLUSION Hypersecretion of TNF is shownto be one of the major causes of lung injuryduring ANP;'Tong Xia'purgative method couldalleviate the degree of lung injury mediated byTNF.展开更多
The pathogenesis of severe acute pancreatitis isvery complicated.It is a multifactorial as well asmultifaceted disease.First of all,the etiologicagents initiate the pancreatic acinar injury by releaseof pancreatic enz...The pathogenesis of severe acute pancreatitis isvery complicated.It is a multifactorial as well asmultifaceted disease.First of all,the etiologicagents initiate the pancreatic acinar injury by releaseof pancreatic enzymes and overstimulation ofmacrophages and neutrophils,then the cytokinesand inflammatory mediators are liberated.There展开更多
1材料和方法1.1材料为1997-09/1998—12随机获得的于发病24 h 内收住的急性胰腺炎(AP)患者41例,按有关 AP 严重度临床分类标准分为轻症组(MAP 22例,男9例,女13例,平均年龄50.9岁),重症组(SAP 19例,男8例,女11例,平均年龄53.6岁),后者进...1材料和方法1.1材料为1997-09/1998—12随机获得的于发病24 h 内收住的急性胰腺炎(AP)患者41例,按有关 AP 严重度临床分类标准分为轻症组(MAP 22例,男9例,女13例,平均年龄50.9岁),重症组(SAP 19例,男8例,女11例,平均年龄53.6岁),后者进一步分为 SAP Ⅰ级和 SAPⅡ级,均为非手术治疗,方案相同.对照组为健康体检及献血人员20例,男7例,女13例,平均年龄49.75岁.各组间在年龄、性别上无显著性差异.展开更多
非胆源性复发性重症急性胰腺炎,病因复杂,彻底治疗困难,我院1991-01/1999-09共收治36例,现将其治疗体会分析如下.1材料和方法本组男11例,女25例,年龄17岁～76岁,平均52岁.病史中2次发作者19例,3次或3次以上者17例,曾有引流术史的5例,近...非胆源性复发性重症急性胰腺炎,病因复杂,彻底治疗困难,我院1991-01/1999-09共收治36例,现将其治疗体会分析如下.1材料和方法本组男11例,女25例,年龄17岁～76岁,平均52岁.病史中2次发作者19例,3次或3次以上者17例,曾有引流术史的5例,近期复发(3 mo 以内)14例,中远期(0.5 a～3 a)复发者22例.诊断依据:急性胰腺炎病史;临床症状和体征;展开更多
目的:探讨清胰利胆颗粒联合床旁连续性肾脏替代治疗(CRRT)对重症急性胰腺炎(SAP)患者炎性因子、免疫功能及外周血叉状头/翅膀状螺旋转录因子(Foxp3)、维甲酸相关孤独受体γt(RORγt)m RNA表达的影响。方法:选取2017年3月~2019年12月期...目的:探讨清胰利胆颗粒联合床旁连续性肾脏替代治疗(CRRT)对重症急性胰腺炎(SAP)患者炎性因子、免疫功能及外周血叉状头/翅膀状螺旋转录因子(Foxp3)、维甲酸相关孤独受体γt(RORγt)m RNA表达的影响。方法:选取2017年3月~2019年12月期间我院收治的SAP患者80例,根据随机数字表法分为对照组(n=40)和研究组(n=40),对照组患者予以床旁CRRT疗法治疗,研究组在对照组的基础上联合清胰利胆颗粒治疗,比较两组疗效、炎性因子、免疫功能、外周血Foxp3、RORγt mRNA相对表达量及不良反应。结果:研究组治疗后临床总有效率为87.50%(35/40),高于对照组患者的70.00%(28/40)(P<0.05)。研究组治疗后免疫球蛋白G(Ig G)、免疫球蛋白A(Ig A)、免疫球蛋白M(Ig M)、Foxp3 m RNA相对表达量较治疗前升高,且高于对照组(P<0.05)。两组治疗后白介素-6(IL-6)、白介素-8(IL-8)、肿瘤坏死因子-α(TNF-α)、RORγt m RNA相对表达量较治疗前降低,且研究组低于对照组(P<0.05)。两组在治疗过程中均未出现严重不良反应。结论:清胰利胆颗粒联合床旁CRRT疗法治疗SAP,疗效显著,可有效改善患者炎性因子、免疫功能,其主要作用机制可能与外周血Foxp3、RORγt m RNA相对表达量有关。展开更多
基金the"8th 5-year Plan"of National Administration Bureau of Traditional Chinese Medicine and Pharmacy,No.H09301
文摘AIM To investigate the role of tumor necrosisfactor(TNF)in lung injury during acutenecrotizing pancreatitis(ANP),and thetherapeutic effect of'Tong Xia'purgativemethod in minimizing the severity of lung injury.METHODS Fourteen canines were randomlydivided into 3 groups:the'Tong Xia'treatmentgroup(n = 5)using Dachengqitang;salinecontrol group(n = 5),and the sham operationgroup(n = 4).TNF activity in serum and inbronchoalveolar lavage fluid(BALF),the serumendotoxin levels were measured,and theseverity of lung injury evaluated.RESULTS Elevation of TNF activity was moreprominent in BALF than in serum.TNF activity inserum at 6 and 12 hours and in BALI:wassignificantly decreased in the'Tong Xia'treatment group than in the saline control one(q=21.11,q=12.07,q=9.03,respectively,P【0.01)and the lung injury was significantlyalleviated at 12 hours as compared with that inthe saline group,manifested as amelioration otthe lung wet/ dry weight ratio,decrease inprotein concentration and neutrophils count inBALF,and improvement of pulmonaryinflammatory changes.A positive correlationwas demonstrated between serum TNF activity and endotoxin level.CONCLUSION Hypersecretion of TNF is shownto be one of the major causes of lung injuryduring ANP;'Tong Xia'purgative method couldalleviate the degree of lung injury mediated byTNF.
文摘The pathogenesis of severe acute pancreatitis isvery complicated.It is a multifactorial as well asmultifaceted disease.First of all,the etiologicagents initiate the pancreatic acinar injury by releaseof pancreatic enzymes and overstimulation ofmacrophages and neutrophils,then the cytokinesand inflammatory mediators are liberated.There
文摘1材料和方法1.1材料为1997-09/1998—12随机获得的于发病24 h 内收住的急性胰腺炎(AP)患者41例,按有关 AP 严重度临床分类标准分为轻症组(MAP 22例,男9例,女13例,平均年龄50.9岁),重症组(SAP 19例,男8例,女11例,平均年龄53.6岁),后者进一步分为 SAP Ⅰ级和 SAPⅡ级,均为非手术治疗,方案相同.对照组为健康体检及献血人员20例,男7例,女13例,平均年龄49.75岁.各组间在年龄、性别上无显著性差异.
文摘非胆源性复发性重症急性胰腺炎,病因复杂,彻底治疗困难,我院1991-01/1999-09共收治36例,现将其治疗体会分析如下.1材料和方法本组男11例,女25例,年龄17岁～76岁,平均52岁.病史中2次发作者19例,3次或3次以上者17例,曾有引流术史的5例,近期复发(3 mo 以内)14例,中远期(0.5 a～3 a)复发者22例.诊断依据:急性胰腺炎病史;临床症状和体征;
文摘目的:探讨清胰利胆颗粒联合床旁连续性肾脏替代治疗(CRRT)对重症急性胰腺炎(SAP)患者炎性因子、免疫功能及外周血叉状头/翅膀状螺旋转录因子(Foxp3)、维甲酸相关孤独受体γt(RORγt)m RNA表达的影响。方法:选取2017年3月~2019年12月期间我院收治的SAP患者80例,根据随机数字表法分为对照组(n=40)和研究组(n=40),对照组患者予以床旁CRRT疗法治疗,研究组在对照组的基础上联合清胰利胆颗粒治疗,比较两组疗效、炎性因子、免疫功能、外周血Foxp3、RORγt mRNA相对表达量及不良反应。结果:研究组治疗后临床总有效率为87.50%(35/40),高于对照组患者的70.00%(28/40)(P<0.05)。研究组治疗后免疫球蛋白G(Ig G)、免疫球蛋白A(Ig A)、免疫球蛋白M(Ig M)、Foxp3 m RNA相对表达量较治疗前升高,且高于对照组(P<0.05)。两组治疗后白介素-6(IL-6)、白介素-8(IL-8)、肿瘤坏死因子-α(TNF-α)、RORγt m RNA相对表达量较治疗前降低,且研究组低于对照组(P<0.05)。两组在治疗过程中均未出现严重不良反应。结论:清胰利胆颗粒联合床旁CRRT疗法治疗SAP,疗效显著,可有效改善患者炎性因子、免疫功能,其主要作用机制可能与外周血Foxp3、RORγt m RNA相对表达量有关。
