INTRODUCTIONDouble primary esophageal carcinoma is defined ashaving two loci of squamous cell cancersimultaneously or consecutively developing indifferent sites of esophagus.This rare diseaseappears mostly in the lite...INTRODUCTIONDouble primary esophageal carcinoma is defined ashaving two loci of squamous cell cancersimultaneously or consecutively developing indifferent sites of esophagus.This rare diseaseappears mostly in the literature as case reports,reports about its treatment are even moreinfrequent.Here we present our experiences展开更多
INTRODUCTIONIn China,primary liver cancer (PLC) ranks secondin cancer mortality since the 1990s.In the field ofPLC treatment,surgical resection remains the best,which includes large PLC resection,small PLCresection,re...INTRODUCTIONIn China,primary liver cancer (PLC) ranks secondin cancer mortality since the 1990s.In the field ofPLC treatment,surgical resection remains the best,which includes large PLC resection,small PLCresection,re-resection of subclinical recurrence,aswell as cytoreduction and sequential resection forunresectable PLC.However,recurrence展开更多
自1993年至今采用食管支架置入术治疗食管贲门癌性狭窄及瘘63例,报告如下.1材料和方法1.1材料内镜下组织活检证实的食管贲门癌患者63例,原发53例(其中8例伴食管支气管瘘),术后复发10例,住院全面检查后胸外科会诊已无手术指征.男53例,女1...自1993年至今采用食管支架置入术治疗食管贲门癌性狭窄及瘘63例,报告如下.1材料和方法1.1材料内镜下组织活检证实的食管贲门癌患者63例,原发53例(其中8例伴食管支气管瘘),术后复发10例,住院全面检查后胸外科会诊已无手术指征.男53例,女10例,年龄45岁～83岁,平均53.6岁.均有明显吞咽困难,按 Stool 分级法…(0～Ⅳ级)分为Ⅱ级(进半流质)5例,Ⅲ级(进流质)47例,Ⅳ级(滴水不进)11例.病变上缘距门齿距离<20 cm 9例,20 cm～30 cm 29例,>30 cm 25例.内镜下管腔直径<2 mm13例,2 mm～4 mm 35例,4 mm～5 mm 15例.病变长度2 cm～3 cm 8例,4 cm～6 cm 32例,7 cm～9 cm 16例,>9 cm 7例.展开更多
AIM To observe the tumor inhibitory effects bytransfecting IL-6 cDNA into colon cancer cell lineHT-29 with retroviral vector pZIP cDNA.METHODS Human IL-6 gene was reconstructedin retrovirus vector and transfected into...AIM To observe the tumor inhibitory effects bytransfecting IL-6 cDNA into colon cancer cell lineHT-29 with retroviral vector pZIP cDNA.METHODS Human IL-6 gene was reconstructedin retrovirus vector and transfected into incasingcells PA317 by lipofectamine mediated method,the clones of the cells transferred with hlL-6were selected by G418,and targeted HT-29 cellswere infected with the virus granules secretedfrom PA317 and also selected by G418.Test genetranscription and expression level byhybridization,ELISA and MTT assay,etc.Analyze tumor inhibitory effects according to thecell growth curve,plating forming rate andtumorigenicity in nude mice.RESULT Successfully constructed andtransfected recombinant expressing vectorspZIPIL-6 cDNA and got positive transfected celllines.The colon cancer cell line(HT-29 IL-5)transfected with the hlL-5 gene by retroviralvector was established.The log proliferationperiod and the doubling time of this cell line wasbetween 4 to 7 days and 2.5 days according tothe direct cell count,the cell proliferation wasobviously inhibited with MTT assay,the platinginhibitory rate was 50% by plating efficiencytest.When HT-29 IL-6 cells were inoculated intothe nude mice subcutaneously,carcinogenicactivity of the solid tumor was found superior tothe control group and the size of tumor was notsignificantly enlarged.Injection of combinationvirus fluid containing 11.-6 gene intotransplantation tumors could inhibit the growthand development of the tumor.CONCLUSION IL-6 could inhibit the growth andproliferation of colon cancer cells by retroviralvector-mediated transduction.展开更多
文摘INTRODUCTIONDouble primary esophageal carcinoma is defined ashaving two loci of squamous cell cancersimultaneously or consecutively developing indifferent sites of esophagus.This rare diseaseappears mostly in the literature as case reports,reports about its treatment are even moreinfrequent.Here we present our experiences
文摘INTRODUCTIONIn China,primary liver cancer (PLC) ranks secondin cancer mortality since the 1990s.In the field ofPLC treatment,surgical resection remains the best,which includes large PLC resection,small PLCresection,re-resection of subclinical recurrence,aswell as cytoreduction and sequential resection forunresectable PLC.However,recurrence
文摘自1993年至今采用食管支架置入术治疗食管贲门癌性狭窄及瘘63例,报告如下.1材料和方法1.1材料内镜下组织活检证实的食管贲门癌患者63例,原发53例(其中8例伴食管支气管瘘),术后复发10例,住院全面检查后胸外科会诊已无手术指征.男53例,女10例,年龄45岁～83岁,平均53.6岁.均有明显吞咽困难,按 Stool 分级法…(0～Ⅳ级)分为Ⅱ级(进半流质)5例,Ⅲ级(进流质)47例,Ⅳ级(滴水不进)11例.病变上缘距门齿距离<20 cm 9例,20 cm～30 cm 29例,>30 cm 25例.内镜下管腔直径<2 mm13例,2 mm～4 mm 35例,4 mm～5 mm 15例.病变长度2 cm～3 cm 8例,4 cm～6 cm 32例,7 cm～9 cm 16例,>9 cm 7例.
文摘AIM To observe the tumor inhibitory effects bytransfecting IL-6 cDNA into colon cancer cell lineHT-29 with retroviral vector pZIP cDNA.METHODS Human IL-6 gene was reconstructedin retrovirus vector and transfected into incasingcells PA317 by lipofectamine mediated method,the clones of the cells transferred with hlL-6were selected by G418,and targeted HT-29 cellswere infected with the virus granules secretedfrom PA317 and also selected by G418.Test genetranscription and expression level byhybridization,ELISA and MTT assay,etc.Analyze tumor inhibitory effects according to thecell growth curve,plating forming rate andtumorigenicity in nude mice.RESULT Successfully constructed andtransfected recombinant expressing vectorspZIPIL-6 cDNA and got positive transfected celllines.The colon cancer cell line(HT-29 IL-5)transfected with the hlL-5 gene by retroviralvector was established.The log proliferationperiod and the doubling time of this cell line wasbetween 4 to 7 days and 2.5 days according tothe direct cell count,the cell proliferation wasobviously inhibited with MTT assay,the platinginhibitory rate was 50% by plating efficiencytest.When HT-29 IL-6 cells were inoculated intothe nude mice subcutaneously,carcinogenicactivity of the solid tumor was found superior tothe control group and the size of tumor was notsignificantly enlarged.Injection of combinationvirus fluid containing 11.-6 gene intotransplantation tumors could inhibit the growthand development of the tumor.CONCLUSION IL-6 could inhibit the growth andproliferation of colon cancer cells by retroviralvector-mediated transduction.
