Experimental Study of Submergence Ratio on Local Scour Around a Square Pile in Steady Flow

Scour around a submerged square pile was realized experimentally in a steady flow to study the effects of flow depth on local scour.Flow depth to pile height ratios ranging from 1.5 to 5 in uniform sand and 2 to 5 in non-uniform sand were tested in the approaching flow velocity to critical velocity(larger than which the sediment particle is motivated)ratios of 0.56 and 1.03,respectively.The influences of flow depth were investigated on the basis of analysis of the three-dimensional topography,temporal maximum scour depth,bed profile development,and equilibrium scour depth.Results showed that the maximum scour depth was at the upstream corners of the pile other than at the stagnation point.The evolutions of the maximum scour depth data in non-uniform sand were well fitted with a recent exponential function,which characterized the initial,developing,and equilibrium stages of scour depth.The scour hole slopes upstream of the pile were found to be parallel to each other in the process of each test and were mainly governed by the sediment repose underwater.The equilibrium scour depth varied slightly with flow depth when the submergence ratio was larger than 1 in uniform sand while it was 2 in non-uniform sand.The armoring effects of coarse sediment particles markedly reduced the sediment transport in non-uniform sand despite the 0.34 increment in non-uniformity.

Morpho-Physiological Changes in Roots of Rice Seedling upon Submergence

Submergence is a serious environmental condition that causes large loss in rice production in rain fed lowland and flood affected area. This study evaluated morphological and physiological responses of rice roots to submergence using two tolerant rice genotypes FR13A and Swarna-Sub 1 and two sensitive ones Swarna and IR42. The tolerant genotypes had higher survival rate and less shoot elongation but greater root elongation during submergence than the sensitive ones. After submergence,the tolerant genotypes also had higher root dry weight and more active roots than the sensitive ones.Tolerant genotypes exhibited less root injury, with less malondialdehyde production and slower electrolyte leakage after submergence. Tolerant genotypes also maintained higher concentrations of soluble sugar and starch in roots and shoots and higher chlorophyll retention after submergence than the sensitive ones.Our data showed that root traits such as root activity and root growth are associated with survival rate after submergence. This is probably accomplished through higher energy supply, and membrane integrity is necessary to preserve root function and reduce injury during submergence. These root traits are important for submergence tolerance in rice.

Ethylene response factor BnERF2-like (ERF2.4) from Brassica napus L.enhances submergence tolerance and alleviates oxidative damage caused by submergence in Arabidopsis thaliana

Ethylene response factor proteins play an important role in regulating a variety of stress responses in plants,but their exact functions in submergence stress are not well understood.In this study,we isolated BnE RF2.4 from Brassica napus L.to study its function in submergence tolerance.The expression of the BnE RF2.4 gene in B.napus and the expression of antioxidant enzyme genes in transgenic Arabidopsis were analyzed by quantitative RT-PCR.The expression of BnE RF2.4 was induced by submergence in B.napus and the overexpression of BnE RF2.4 in Arabidopsis increased the level of tolerance to submergence and oxidative stress.A histochemical method detected lower levels of H_2O_2,O^(·-)_2and malondialdehyde(MDA) in transgenic Arabidopsis.Compared to the wild type,transgenic lines also had higher soluble sugar content and higher activity of antioxidant enzymes,which helped to protect plants against the oxidative damage caused by submergence.It was concluded that BnE RF2.4 increased the tolerance of plants to submergence stress and may be involved in regulating soluble sugar content and the antioxidant system in defense against submergence stress.

Marker-Assisted Breeding of Thermo-Sensitive Genic Male Sterile Line 1892S for Disease Resistance and Submergence Tolerance

Rice line 1892S is an elite thermo-sensitive genic male sterile(TGMS)line for two-line hybrid rice production.However,1892S is susceptible to rice blast,bacterial blight and submergence.Here we reported the introduction of blast resistance(R)gene Pi9,bacterial blight R gene Xa21 and submergence tolerance gene Sub1A into 1892S genetic background through backcrossing and marker-assisted selection.The improved TGMS line 31892S and its hybrids conferred disease resistance to rice blast and bacterial blight,and showed submergence tolerance for over 14 d without significant loss of viability.The sterility-fertility conversion of 31892S was similar to that of 1892S.31892S and its derived hybrid rice had similar agronomic traits and grain quality with 1892S and the control hybrid rice,respectively.The newly developed 31892S provided an improved TGMS line for two-line hybrid rice production with disease resistance to rice blast and bacterial blight,and submergence tolerance with no yield penalty or change in grain quality.

Assessment of Variation in Morpho-Physiological Traits and Genetic Diversity in Relation to Submergence Tolerance of Five Indigenous Lowland Rice Landraces

The present study evaluated submergence responses in 88 lowland indigenous rice(Oryza sativa L.) landraces from Koraput, India, to identify submergence-tolerant rice genotypes. In pot experiments, variations in survival rate, shoot elongation, relative growth index, dry matter, chlorophyll, soluble sugar and starch contents were evaluated in two consecutive years under well-drained and completely submerged conditions. Principal component analysis showed that the first three axes contributed 96.820% of the total variation among the landraces, indicating wide variation between genotypes. Major traits such as survival rate, relative growth index, soluble sugar and starch contents appeared to be important determinants of phenotypic diversity among the landraces. Phenotypic coefficient of variance was higher than genotypic coefficient of variance for all the traits and all showed high heritability(90.38%–99.54%). Five rice landraces(Samudrabali, Basnamundi, Gadaba, Surudaka and Dokarakuji) were the most tolerant to submergence. When submerged for up to 14d, Samudrabali, Basnamundi and Godoba were notable for having greater survival rates than a standard submergence tolerant variety FR13 A, and also notable for elongating more vigorously and accumulating more biomass. These three landraces may therefore be especially useful in lowland rice growing areas that are affected by both moderate stagnant water and flash flooding. Molecular genotyping revealed that the submergence tolerance of Samudrabali, Basnamundi and Godoba is linked to the presence of one or more different Sub1 loci and it may well prove useful for breeding improved submergence tolerant rice varieties, thereby assising to improve yield stability in the rainfed lowland agro-ecosystem.

Physio-Biochemical and Genetic Exploration for Submergence Tolerance in Rice (<i>Oryza sativa</i>L.) Landraces with Special References to <i>Sub</i>1 Loci

In the present study a group of four indigenous and less popular rice genotypes (Meghi, Panibhasha, Jabra and Sholey) reported by growers as submergence tolerant lines from flood prone areas of south Bengal were explored through study of nodal anatomy, physio-biochemical screening under submergence and genotyping with submergence tolerance linked rice microsatellite loci (RM loci). To identify the different allelic forms of different Sub1 compnents (Sub1A, Sub1B and Sub1C) among the studied lines, the genomic DNA of individual genotypes was amplified with three ethylene response factor like genes from Sub1 loci, located on rice chromosome 9. From the different physio-biochemical experiments performed in this investigation, it has been shown that Meghi and Jabra are the two probable potent genotypes which share common properties of both submergence tolerant and deep water nature whereas rest two genotypes (Sholey and Panibhasha) behave like typical deep water rice. The submergence tolerance property of Meghi was also confirmed from submergence tolerance linked SSR based genotyping by sharing with FR13A for some common alleles as reflected in fingerprint derived dendrogram. The rest of the genotypes shared a number of alleles and were included in a separate cluster. The common behaviour of Meghi and FR13A under submergence was also confirmed from genetic study of Sub1 loci through sharing of some common alleles for three Sub1 components (Sub1A, Sub1B and Sub1C loci). One SSR loci (RM 285) was identified as a potent molecular marker for submergence tolerance breeding programme involving these two selected rice lines (Meghi and Jabra) as donor plant through marker assisted selection.

Assessment of Some Biomarkers under Submergence Stress in Some Rice Cultivars Varying in Responses

Three rice varieties, significantly differed in their ability, when subjected to submergence have been studied in relation to physiological attributes. On account of oxidative stress, MDA content and carbonyl content were measured. The MDA content was maximally decreased in FR13A and minimally decreased in Swarna irrespective of shoots and roots. A higher increase in carbonyl content was found in Swarna followed by FR13A and Swarna Sub1A in both shoots and roots. The activity of antioxidant moieties like total phenolics content and flavonoid content were more increased under submergence than that in air except for Swarna. FR13A showed maximum increase in Phenolics and flavonoid content in both shoots and roots when subjected to submergence. A sharp increase in guaiacol peroxidase and glutathione reductase characterized the plants’ response to sub-mergence irrespective of varieties. The expression of Guaiacol peroxidase was increased in FR13A followed by Swarna Sub1A and Swarna. Glutathione reductase was measured in terms of oxidation of NADP(H) and both FR13A and Swarna Sub1A recorded maximum oxidation than Swarna under submergence. With regards to isozymic variation plants were differed to the intensities of poly-peptide, however not in numbers and may be suggestive for concomitant gene expression to sub-mergence. The analysis clarification for possible biomarkers with regards to cellular responses of rice plants under submergence has been anticipated.

Improved Management Options for Submergence-Tolerant (Sub1) Rice Genotype in Flood-Prone Rainfed Lowlands of West Bengal

Flash flood or submergence is a common phenomenon in rice growing rainfed lowland areas that seriously affects crop establishment leading to severe yield losses. A few submergence-tolerant rice varieties have been developed by introgressing SUB1 gene into mega rice varieties of South Asia. Two of these, Swarna-Sub1 and Sambha Mahsuri-Sub1, are already released in India for the commercial cultivation. Performance of these varieties can be further enhanced through adoption of appropriate management practices both in nursery and in main field. Two on-station field experiments were conducted using Swarna-Sub1 during the wet season (kharif) of 2011 at Rice Research Station, Chinsurah, Hooghly, West Bengal (India). Results of these experiments revealed that the use of lower seeding density (25 g m-2), application of balanced doses of N-P2O5-K2O @ 80-40-40 kg ha-1 in nursery and transplanting of aged seedlings (44 days) significantly improved plant survival, yield attributing traits and grain yield. An additional N-dose of 20 kg ha-1 at 7 days after receding of flood water resulted in better post-submergence recovery and maximum grain yield. In the on-farm trials conducted at three different locations in West Bengal, nursery raising of seedlings with the application of N-P2O5-K2O @ 80-40-40 kg ha-1, transplanting them at an optimum age (35 days) and application of 20 kg·N ha-1 after 7 days of de-submergence produced maximum grain yield of Swarna-Sub1.

Application of Marker Assisted Backcrossing to Introgress the Submergence Tolerance QTL <i>SUB</i>1 into the Vietnam Elite Rice Variety-AS996

The result of the study contributes to enhancing and sustaining future livelihoods and food security in Vietnam vis-a-vis climate change. An innovative strategy based on marker-assisted backcrossing (MABC) was used to transform popular rice variety AS996 into the one can tolerate submergence while maintaining its original characteristics preferred by farmers and consumers. The submergence tolerance QTL SUB1 counts for up to 70% of the submergence tolerant and provides a marked improvement of submergence tolerance in all genetic backgrounds and environments tested so far. Parental diversity was carried out with 460 markers. Of which, 53 polymorphic markers were used for assessment on BC1F1, BC2F1 and BC3F1 generations. The best BC1F1 plant was P422 with highest recipient allele was 87.5%, while the additional chosen plants were P412, P428, P215 and P39 (81% - 84%). All these plants were used to develop BC2F1 generation. The six BC2F1 plants were used to develop BC3F1 and BC2F2 were the plants number P422-11 and P422-14 having 93.75% recipient alleles and P422-12, P422-3, P39-17, P39-25 having 92.25% recipient alleles. Total of 445 BC3F1 plants were confirmed the introgresion of SUB1 using ART5 and SC3. After three generations of backcrossing, application of MABC resulted in the best BC3F1 individual P422-14-177 with 100% of recipient alleles based on the number of 53 markers used with only the introgression size of SUB1 was 0.3Mb between ART5 and SC3. Phenotyping was carried out on BC3F1 and BC2F2 of the selected lines. The survival ratio of these selected lines and IR64SUB1 were the same. It convinced the successfully introgress SUB1 into AS996 rice variety. The breeding line BC4F1 having 100% genetic background of donor variety is ready for develop new submergence tolerant rice variety ASS996-SUB1 to cope with climate change.

Submergence Analysis Using Geo-Informatics Technology for Proposed Dam Reservoirs of Par-Tapi-Narmada River Link Project, Gujarat State, India

The Par-Tapi-Narmada river link envisages transfer of surplus water from west flowing rivers between Par and Tapi in Gujarat State, India to water deficit areas in North Gujarat. The scheme is located mainly in southern Gujarat but it also covers part of the areas of Maharashtra, North of Mumbai on the Western Ghats of India. The main aim of Par-Tapi-Narmada link is to transfer the surplus waters of Par, Auranga, Ambica and Purna River basins to take over part of Narmada Canal command (Miyagam branch) after providing enroute irrigation. It is proposed that water saved in Sardar Sarovar Project, as a result of this transfer, would be taken further northwards to benefit water scarce areas of north Gujarat and also westwards in Saurashtra and Kutch regions. The construction of seven reservoirs on Par-Tapi-Narmada River Link Project would affect land use/land cover, settlements and infrastructure facilities within and around reservoir area. Thus, the submergence impact analysis of all the seven reservoirs of this project have been carried out by using remote sensing and GIS techniques for planning and designing of the structures. Out of the seven reservoirs, the paper discusses submergence analysis of Kelwan Dam reservoir which is located in geologically complex region of the Dangs district. The study attempts to assess the present problems of submergence of land, forest, agriculture, settlements and infrastructure facilities by using GIS techniques for taking alternative remedial measures prior and during construction of the dams.

Determining Critical Submergence in Tanks by Means of Reynolds &Weber Numbers

Critical submergence in pumping systems can be determined using a number of calculations, all of which result from heterogeneous geometries based on water. The most widely spread critical submergence formula is that of the Hydraulic Institute. A study, carried out in Germany, looked at eight different formulations used to calculate critical submergence, comparing their results with those of a hydraulic model test. The conclusion is that the simplest models, overestimate the critical submergence. Similarly, a study of submergence in water intake structures concluded that predicted values were much higher than real values. A detailed analysis has been done to detect the origin of the off-set between the measured submergence and the calculated value. The main aspects selected from the analysis were the fluid properties involved in the surface deformation and the dynamic behavior outlet flow, so two a-dimensional numbers have been selected, Weber and Reynolds. To build an equation, to calculate the critical submergence, based on the mentioned a-dimensional numbers, a mixed technique (numerical and testing) has been used. The first step was driving a test in a hydraulic model to verify the critical submergence level. Then, a numerical model was built to simulate the same phenomenon and calibrate it, to be used in the future. After that, the second step is to simulate and calculate the critical submergence with other boundary condition (fluid, flow rate, pipe diameter). Once the critical submergence is calculated, a non-linear least squared approach has been developed to build the equation to calculate the critical submergence based on the Reynolds and Weber number. The numerical method used in this paper is a finite element model with a fluid volume scheme, used normally in the fluid simulation activities.

对称系泊半潜平台在各种可能系泊组合下的动力学分析

In this technologically advancing world,the demand for more energy,oil and gas production is rapidly escalating.To accomplish this,people have inclined more towards completely floating offshore structures,deployed in deep waters.A semi-submersible is selected in the present study,due to its better response characteristics and stability under harsh environmental conditions.The semi-submersible is position restrain with spread mooring lines incorporated with submerged buoy at different locations has been studied.A detailed numerical analysis is carried out using Ansys Aqwa for dynamic response analysis of semi-submersible under the combination of wind,wave,and current forces for 0°,45°,and 90°directions.It was observed that damping ratios and natural periods had been affected based on the position and number of submerged buoys in the mooring system.Also,reduction in mooring force after incorporating buoy in the mooring lines was observed.Subsequently,a Matlab code based on the S-N curve approach was generated and employed to investigate the fatigue damage of mooring lines under dynamic variation of mooring forces.When pegged with submerged buoys,fatigue life of mooring lines is increased under intact and postulated damaged mooring conditions.Moreover,coupling of motion responses of semi-submersible is observed,and unbounded response is not seen in any degrees-offreedom,even during damaged condition of mooring lines.

Stabilisation of estuarine sediments with an alkali-activated cement for deep soil mixing applications

In this work,an alternative alkali-activated cement(AAC)made of ladle slag precursor mixed with sodium hydroxide and sodium silicate has been developed to enhance the bearing capacity of estuarine soils in coastal conditions via deep soil mixing(DSM).The AAC was optimized to use a low reactivity precursor(ladle slag)and to deal with a contaminated high-water content natural sediment cured under water.The material performance was analysed by comparison to a mixture made with Portland cement and cured in the same conditions.Flexural and unconfined compressive strength tests as well as seismic waves measurements after 3-,7-,14-and 28-d curing were performed to obtain a relationship between elastic stiffness and strength with curing time for both mixtures.Remarkably,the AAC mix demonstrated superior strength results,exhibiting almost double flexural and compressive strengths after 28 d compared to the Portland cement mix.The AAC mix also showed a higher rate of stiffness increase than the Portland cement mix,which has a higher initial stiffness at young ages but lower stiffness evolution.Leachate analysis confirmed that the proposed AAC could effectively immobilise any contaminants from soil or precursors.The effect of curing under stress was analysed in triaxial compression tests and found to be insignificant,indicating that laboratory data obtained without stress curing can represent the material's behaviour in a DSM column,which will cure under the weight of the column.

Investigation of hydroxyl-terminated polybutadiene propellant breaking characteristics and mechanism impacted by submerged cavitation water jet

A submerged cavitation water jet(SCWJ)is an effective method to recycle solid propellant from obsolete solid engines by the breaking method.Solid propellant's breaking modes and mechanical process under SCWJ impact are unclear.This study aims to understand those impact breaking mechanisms.The hydroxyl-terminated polybutadiene(HTPB)propellant was chosen as the research material,and a self-designed test system was used to conduct impact tests at four different working pressures.The high-speed camera characterized crack propagation,and the DIC method calculated strain change during the impact process.Besides,micro and macro fracture morphologies were characterized by scanning electron microscope(SEM)and computed tomography(CT)scanning.The results reveal that the compressive strain concentration region locates right below the nozzle,and the shear strain region distributes symmetrically with the jet axis,which increases to 4% at first 16th ms,the compressive strain rises to 2% and 6% in the axial and transverse direction,respectively.The two tensile cracks formed first at the compression strain concentrate region,and there generate many shear cracks around the tensile cracks,and those shear cracks that develop and aggregate cause the cracks to become wider and cut through the tensile cracks,forming the tensile-shear cracks and the impact parts eventually fail.The HTPB propellant forms a breaking hole shaped conical after impact 10 s.The mass loss increases by 17 times at maximum,with the working pressure increasing by three times.Meanwhile,the damage value of the breaking hole remaining on the surface increases by 7.8 times while 2.9 times in the depth of the breaking hole.The breaking efficiency is closely affected by working pressures.The failure modes of HTPB impacted by SCWJ are classified as tensile crack-dominated and tensile-shear crack-dominated damage mechanisms.

Numerical Analysis of the Influence of the Impinging Distance on the Scouring Efficiency of Submerged Jets

Water jet technology is widely used in submerged buried pipes as a non-traditional trenching process,often invol-ving a complex sediment response.An important adjustable and influential engineering variable in this technol-ogy is represented by the impinging distance.In this study,the FLOW-3D software was used to simulate the jet scouring of sand beds in a submerged environment.In particular,four sets of experimental conditions were con-sidered to discern the relationship between the maximum scour depth and mass and the impinging distance.As shown by the results,a critical impinging distance h0 exists by which the static scour depth can be maximized;the scour mass ratio between dynamic and static conditions decreases as the impinging distance increases.Moreover,the profile contours are similar when the erosion parameter Ec is in the range 0.35<Ec<2.Empirical equations applicable for predicting the jet trenching contour under both dynamic and static scour modes are also provided in this study.

Structure Design of Underwater Micro Robot

A submergible robot model has been presented, and for 3D printing measures, their parts have been modified enough. It has been shown in our design that using printable connectors—a few engines and weight arrangements can be carried out, permitting distinctive moving prospects. After presenting our configuration and delineating a bunch of potential structures, a helpful model dependent on open-source equipment and programming arrangements has been presented conditionally. The model can be effectively tried in a few makes-a plunge streams and lakes throughout the planet. The unwavering quality of the printed models can be strained distinctly in generally shallow waters. Nonetheless, we accept that their accessibility will inspire the overall population to construct and test submerged robots, subsequently accelerating the improvement of imaginative arrangements and applications.

Evaluation of airlift pump performance for vertical conveying of coal particles

One of the crucial aspects of reducing air consumption when conveying particles with an airlift pump is to know the factors that affect the process of particle motion at an initial velocity of zero.To determine the influence of submer-gence ratio and physical properties of particles(such as size,shape,and mass)on the onset of vertical particle motion,the airlift pump was taken as the research object,and spherical glass together with irregular shaped coal were used as experimental test particles.The results show that unlike the water-solid environment,the start of particle motion in the water-air mixture does not always occur at a certain value of superficial water velocity and this value also increases with increasing submergence level.Among the parameters considered,the role of submergence ratio is much more effective than the dimensions and the shape of the particle,because by increasing submergence from 0.3 to 0.8,it is possible to reduce air consumption by up to 8 times.Based on this study the corresponding theoretical model derived by Fujimoto et al.is optimized,wherein the overall agreement between the modified theory and present experimental data is particularly good.Contrary to Fujimoto,the minimum superficial water velocity for lifting solids in the air-water mixture is not always smaller than water ambient which indicates on optimum submergence ratio higher than 0.7.Finally,a new criterion was introduced to describe the moment of onset of the particle motion as a function of the superficial fluid velocity ratio for each submergence value.

Flow and penetration behavior of submerged side-blown gas

To assess the widely used submerged side-blowing in pyrometallurgy,a high-speed camera-digital image processing-statistical approach was used to systematically investigate the effects of the gas flow rate,nozzle diameter,and inclination angle on the space-time distribution and penetration behavior of submerged side-blown gas in an air-water system.The results show that the gas motion gradually changes from a bubbling regime to a steady jetting regime and the formation of a complete jet structure as the flow rate increases.When the flow rate is low,a bubble area is formed by large bubbles in the area above the nozzle.When the flow rate and the nozzle diameter are significant,a bubble area is formed by tiny bubbles in the area above the nozzle.The increased inclination angle requires a more significant flow rate to form a complete jet structure.In the sampling time,the dimensionless horizontal and vertical penetration depths are Gaussian distributed.Decreasing the nozzle diameter and increasing the flow rate or inclination angle will increase the distribution range and discreteness.New correlations for a penetration depth with an error of±20%were obtained through dimensional analysis.The dimensionless horizontal penetration depth of an argon-melt system in a 120 t converter calculated by the correlation proposed by the current study is close to the result calculated by a correlation in the literature and a numerical simulation result in the literature.

Effect of light and nutrients on interspecific interactions between submerged macrophytes:implications for restoration of multispecies aquatic vegetation in eutrophic lakes

Constructing multispecies submerged vegetation systems and maintaining stable seasonal succession is crucial for restoring shallow eutrophic lakes.However,little is known about the interactions between successional and existing species of different growth forms,particularly under the low light and high nutrient conditions of eutrophic lakes.We measured the functional traits of mature Vallisneria natans(Lour.)Hara plants and Potamogeton crispus L.shoots in monoculture and mosaic patterns under different light and nutrient conditions.The effect of light on functional traits of the submerged macrophyte species was more significant than that of nutrients,but the reverse was true for P.crispus biomass allocation.Moreover,interspecific interactions affected only the submerged macrophytes under the low light condition and varied with species.Specifically,the interaction of P.crispus to V.natans was biased towards competition,while the interaction of V.natans to P.crispus was converted from facilitation to competition by eutrophication,particularly in the homogenous mosaic growth pattern.This study demonstrates that sufficient light is a prerequisite and patch planting is an effective means to form a multispecies submerged vegetation system.In addition,we emphasize that the coexistence of eutrophication and low light will likely result in a competition between submerged macrophytes thus simplifying the vegetation,even if their growth forms and growing seasons are different.These findings help explain the collapse of multispecies submerged vegetation and guide the restoration of aquatic plants in eutrophic lakes.

Temporal characteristics of algae-denitrifying bacteria co-occurrence patterns and denitrifier assembly in epiphytic biofilms on submerged macrophytes in Caohai Lake,SW China

Denitrifying bacteria in epiphytic biofilms play a crucial role in nitrogen cycle in aquatic habitats.However,little is known about the connection between algae and denitrifying bacteria and their assembly processes in epiphytic biofilms.Epiphytic biofilms were collected from submerged macrophytes(Patamogeton lucens and Najas marina L.)in the Caohai Lake,Guizhou,SW China,from July to November 2020 to:(1)investigate the impact of abiotic and biotic variables on denitrifying bacterial communities;(2)investigate the temporal variation of the algae-denitrifying bacteria co-occurrence networks;and(3)determine the contribution of deterministic and stochastic processes to the formation of denitrifying bacterial communities.Abiotic and biotic factors influenced the variation in the denitrifying bacterial community,as shown in the Mantel test.The co-occurrence network analysis unveiled intricate interactions among algae to denitrifying bacteria.Denitrifying bacterial community co-occurrence network complexity(larger average degrees representing stronger network complexity)increased continuously from July to September and decreased in October before increasing in November.The co-occurrence network complexity of the algae and nirS-encoding denitrifying bacteria tended to increase from July to November.The co-occurrence network complexity of the algal and denitrifying bacterial communities was modified by ammonia nitrogen(NH_(4)^(+)-N)and total phosphorus(TP),pH,and water temperature(WT),according to the ordinary least-squares(OLS)model.The modified stochasticity ratio(MST)results reveal that deterministic selection dominated the assembly of denitrifying bacterial communities.The influence of environmental variables to denitrifying bacterial communities,as well as characteristics of algal-bacterial co-occurrence networks and the assembly process of denitrifying bacterial communities,were discovered in epiphytic biofilms in this study.The findings could aid in the appropriate understanding and use of epiphytic biofilms denitrification function,as well as the enhancement of water quality.