Streptococcus equi subsp.zooepidemicus(SEZ)is an important zoonotic agent.Here,a virulence-attenuated strain M35246 derived from natural variation of wild-type SEZ ATCC35246 was found.M35246 showed a deletion of 25con...Streptococcus equi subsp.zooepidemicus(SEZ)is an important zoonotic agent.Here,a virulence-attenuated strain M35246 derived from natural variation of wild-type SEZ ATCC35246 was found.M35246 showed a deletion of 25contiguous genes as well as a loss-of-function mutation in covS.Subsequently,a 25-gene-deleted strain(ΔPI),a covS-mutant strain(Mcov S),and relevant complementary strains were constructed and investigated.M35246 and Mcov S were significantly less encapsulated and exhibited poorer anti-phagocytic capacity compared to wild-type SEZ.McovS was significantly more sensitive toβ-lactams,aminoglycosides,macrolides,and lincosamides than wild-type SEZ.M35246,McovS,andΔPI exhibited an increase in median lethal dose(LD_(50))in mice by 10~5,10~5,and 5 times when compared to wild-type SEZ,respectively.Neither M35246 nor McovS were isolated from mice 48 h after being challenged with approximately 2000 times the LD_(50)of wild-type SEZ.Transcriptome analysis showed that 668 significantly differentially expressed genes existed between McovS and wild-type SEZ.Numerous virulence factor-encoding genes and anabolicrelated genes in McovS that were involved in anti-phagocytosis,capsule formation,pathogenicity,and antibiotic resistance were downregulated significantly relative to the wild-type strain.This study revealed that the CovS plays a vital role in the establishment of SEZ virulence.展开更多
Due to climate change,it is necessary to develop plant varieties that are resilient to climate conditions and resistant to abiotic and biotic stresses.The use of microalgae,which are microorganisms that contain carboh...Due to climate change,it is necessary to develop plant varieties that are resilient to climate conditions and resistant to abiotic and biotic stresses.The use of microalgae,which are microorganisms that contain carbohydrates,proteins,lipids,and vitamins,against drought tolerance is a new approach.The aim of the current study was to determine the drought-related mechanisms in the conomon melon genotype and develop drought-tolerant melon cultivars.Morphological,physiological,pomological,and molecular analyses were carried out on the algae-treated genotypes.It has been determined that commercial algae application provides the best results in leaf temperature,leaf relative water contents(LRWC),plant height,fruit length,fruit diameter,and yield,while Cag Cag(a special river in the region)stream algae application gives better results in main stem diameter.It was determined that the number of nodes in the control(without algae)plots was higher than in the other two treatments.Yield and LRWC and plant height values of genotype 7 were the best values among other genotypes.The leaf temperature measurement was lower on genotype 9 than on the other genotypes.While the highest fruit length value was measured in genotype 1,genotype 8 was superior in the main stem diameter,fruit diameter,and the number of nodes among the remaining plant materials.In terms of yield,it was determined that the interaction between genotype 2 and the commercial algae resulted in the best outcomes.In addition,the results of the genetic evaluation revealed that the materials used were not genetically distant from each other and more detailed genetic evaluations are needed.The molecular kinship analysis revealed that the genotypes used in the study were divided into three distinct groups,with individuals within each group exhibiting a high degree of relatedness to one another.As a result of the study,it was found that the application of microalgae had significant effects on improving the drought tolerance of Cucumis melo subsp.agrestis var.conomon genotypes.展开更多
A new species of </span><i><span style="font-family:Verdana;">Gentiana</span></i><span style="font-family:Verdana;"> (</span><i><span style="...A new species of </span><i><span style="font-family:Verdana;">Gentiana</span></i><span style="font-family:Verdana;"> (</span><i><span style="font-family:Verdana;">Gentianaceae</span></i><span style="font-family:Verdana;">), </span><i><span style="font-family:Verdana;">Gentiana</span></i> <i><span style="font-family:Verdana;">ciliolata</span></i> <span style="font-family:Verdana;">is</span><span style="font-family:Verdana;"> described and illustrated;it has a spectacular characteristics of calyx lobe ciliolate, distributed in Yunnan and Sichuan Provinces on the two sides of Jinsha River, and then two subspecies have been further re-classified as </span><i><span style="font-family:Verdana;">Gentiana</span></i> <i><span style="font-family:Verdana;">ciliolata</span></i> <span style="font-family:Verdana;">subsp</span><i><span style="font-family:Verdana;">.</span></i> <i><span style="font-family:Verdana;">ciliolata</span></i> <span style="font-family:Verdana;">and </span><i><span style="font-family:Verdana;">Gentiana</span></i> <i><span style="font-family:Verdana;">ciliolata</span></i> <span style="font-family:Verdana;">subsp.</span> <i><span style="font-family:Verdana;">longiloba</span></i> <span style="font-family:Verdana;">respectively</span><i><span style="font-family:Verdana;">.</span></i> <span style="font-family:Verdana;">The subsp.</span> <i><span style="font-family:Verdana;">ciliolata</span></i><span style="font-family:Verdana;"> idistributes</span> <span style="font-family:Verdana;">on the right side of Jinsha River in Yiliang County, Yunnan Province and the subsp</span><i><span style="font-family:Verdana;">.</span></i> <i><span style="font-family:Verdana;">longiloba</span></i><span style="font-family:Verdana;"> distributes itself </span><span style="font-family:Verdana;">on the southwest and west part of Sichuan Province, such as Leibo County, Wenchuan County and Mabian Yi Autonomous Prefacture, in the grassland under forests and moist rock slopes at an altitude of 1300 - 3200 m.展开更多
Two new beta-dihydroagarofuran sesquiterpene polyol esters were isolated from Celastrus paniculatus.subsp.paniculatus. Their Structures were deduced on the basis of spectral analyses including 2D NMR. CO2 supercritica...Two new beta-dihydroagarofuran sesquiterpene polyol esters were isolated from Celastrus paniculatus.subsp.paniculatus. Their Structures were deduced on the basis of spectral analyses including 2D NMR. CO2 supercritical fluid was used in separation.展开更多
Coriaria,the only genus under family Coriariacea,receives a lot interest among botanist due to its controversial taxonomic treatment and unique phytochemistry that could be commercially important.The populations of Co...Coriaria,the only genus under family Coriariacea,receives a lot interest among botanist due to its controversial taxonomic treatment and unique phytochemistry that could be commercially important.The populations of Coriaria japonica subsp.intermedia in Cordillera Central Range(CCR),Philippines had never been studied in detail.With the need for more information on this controversial genus,the study deemed it necessary to present the distribution and morpho-anatomical description of C.japonica subsp.intermedia in CCR.A total of 123 populations were documented,found at an elevation range of 1334 to 2494 m asl and stretched between16°31’37.7"to 17°5’47.8"N and 120°39’40.3"to120°55’48"E.Mapping these populations showed a narrow linear pattern of distribution concentrated at the southern portion of CCR.The plant is a shrub with simple leaves,collateral buds,raceme inflorescence,bisexual flowers with persistent perianth forming an accessory fruit after fertilization.In terms of anatomy,much are of the usual dicot characters such as dorsiventral leaf with stomata concentrated in the lower epidermis,eustele with pith in the stem andpresence of secondary growth both on old stem and old root.Interesting findings of the study include the123 populations,the larger leaf measurements,longer petals and larger fruits than the original description given by Matsumura.Also,the paracytic type of stomata and presence of pith in the primary roots of C.japonica subsp.intermedia are interesting since these are common monocot characters while the plant is dicot.These findings are additional information that could help clear the controversies on the genus Coriaria.More importantly,our results could provide essential information for understanding the plant and for developing conservation strategies not just for this plant but also for the other related plants in the CCR biogeographical zone.展开更多
Lactobacillus delbrueckii subsp. bulgaricus, which has been widely used as a fermented milk starter, is a type of probiotic, and certain strains are able to produce exopolysaccharide (EPS). EPS produced from L. bulgar...Lactobacillus delbrueckii subsp. bulgaricus, which has been widely used as a fermented milk starter, is a type of probiotic, and certain strains are able to produce exopolysaccharide (EPS). EPS produced from L. bulgaricus contributes to the physical and biological function of dairy products by regulating immune response, and this tendency seems to place EPS with acidic groups. To date, six types of chemical structure have been determined and are basically composed from glucose (Glc), galactose (Gal), and rhamnose (Rha). Eps clusters on chromosome DNA control the EPS synthesis and are transcribed as one mRNA 14 genes with 18kb on L. bulgaricus Lfi5. Furthermore, L. bulgaricus is able to utilize lactose (Lac) as carbohydrate source, repeating units of EPS are synthesized from Glc 6-phosphate, generated by an Embden-Meyerhof (EM) pathway in cellular carbohydrate assimilation. This review discusses EPS produced from L. bulgaricus.展开更多
It is generally known that the culture for mycoplasma is time-consuming and a variety of nutrients are needed in the culture medium. This brings a lot of difficulties to mycoplasma research and application, including ...It is generally known that the culture for mycoplasma is time-consuming and a variety of nutrients are needed in the culture medium. This brings a lot of difficulties to mycoplasma research and application, including Mycoplasma mycoides subsp. capri(Mmc). Furthermore, little research on the characteristics of Mmc metabolism has been reported. In this study, Mmc PG3 strain cultures were investigated for dynamic gene expression. Culture samples were harvested during logarithmic phase(PG3-1), stationary phase(PG3-2), decline phase(PG3-3) and late decline phase(PG3-4). Twelve RNA samples(three replicates for each of the four growth stages considered) from these cultures were collected and sequenced. Paired comparison between consecutive growth phases in the four growth stages showed 45 significant differentially expressed genes(P〈0.01) were linked to PG3 metabolism. The enzymes these genes coded were mainly involved in ATP synthase, pyrimidine metabolism, nicotinate and nicotinamide metabolism, arginine and proline metabolism. Among these, cytidylate kinase, fructose 1,6-bisphosphate aldolases Class II, nicotinate-nucleotide adenylyltransferase and dihydrolipoamide dehydrogenase play a key role in Mmc metabolism. These results provide a baseline to build our understanding of the metabolic pathway of Mmc.展开更多
基金supported by the National Key Research and Development Program of China(2021YFD1800400)the National Natural Science Foundation of China(31872480)+1 种基金the Jiangsu Agriculture Science and Technology Innovation Fund,China(CX(19)2020)the Priority Academic Program Development of Jiangsu Higher Education Institutions,China(PAPD)。
文摘Streptococcus equi subsp.zooepidemicus(SEZ)is an important zoonotic agent.Here,a virulence-attenuated strain M35246 derived from natural variation of wild-type SEZ ATCC35246 was found.M35246 showed a deletion of 25contiguous genes as well as a loss-of-function mutation in covS.Subsequently,a 25-gene-deleted strain(ΔPI),a covS-mutant strain(Mcov S),and relevant complementary strains were constructed and investigated.M35246 and Mcov S were significantly less encapsulated and exhibited poorer anti-phagocytic capacity compared to wild-type SEZ.McovS was significantly more sensitive toβ-lactams,aminoglycosides,macrolides,and lincosamides than wild-type SEZ.M35246,McovS,andΔPI exhibited an increase in median lethal dose(LD_(50))in mice by 10~5,10~5,and 5 times when compared to wild-type SEZ,respectively.Neither M35246 nor McovS were isolated from mice 48 h after being challenged with approximately 2000 times the LD_(50)of wild-type SEZ.Transcriptome analysis showed that 668 significantly differentially expressed genes existed between McovS and wild-type SEZ.Numerous virulence factor-encoding genes and anabolicrelated genes in McovS that were involved in anti-phagocytosis,capsule formation,pathogenicity,and antibiotic resistance were downregulated significantly relative to the wild-type strain.This study revealed that the CovS plays a vital role in the establishment of SEZ virulence.
基金supported by the Scientific Research Coordinatorship of Siirt University with the Project Number 2018-SİÜFEB-022.
文摘Due to climate change,it is necessary to develop plant varieties that are resilient to climate conditions and resistant to abiotic and biotic stresses.The use of microalgae,which are microorganisms that contain carbohydrates,proteins,lipids,and vitamins,against drought tolerance is a new approach.The aim of the current study was to determine the drought-related mechanisms in the conomon melon genotype and develop drought-tolerant melon cultivars.Morphological,physiological,pomological,and molecular analyses were carried out on the algae-treated genotypes.It has been determined that commercial algae application provides the best results in leaf temperature,leaf relative water contents(LRWC),plant height,fruit length,fruit diameter,and yield,while Cag Cag(a special river in the region)stream algae application gives better results in main stem diameter.It was determined that the number of nodes in the control(without algae)plots was higher than in the other two treatments.Yield and LRWC and plant height values of genotype 7 were the best values among other genotypes.The leaf temperature measurement was lower on genotype 9 than on the other genotypes.While the highest fruit length value was measured in genotype 1,genotype 8 was superior in the main stem diameter,fruit diameter,and the number of nodes among the remaining plant materials.In terms of yield,it was determined that the interaction between genotype 2 and the commercial algae resulted in the best outcomes.In addition,the results of the genetic evaluation revealed that the materials used were not genetically distant from each other and more detailed genetic evaluations are needed.The molecular kinship analysis revealed that the genotypes used in the study were divided into three distinct groups,with individuals within each group exhibiting a high degree of relatedness to one another.As a result of the study,it was found that the application of microalgae had significant effects on improving the drought tolerance of Cucumis melo subsp.agrestis var.conomon genotypes.
文摘A new species of </span><i><span style="font-family:Verdana;">Gentiana</span></i><span style="font-family:Verdana;"> (</span><i><span style="font-family:Verdana;">Gentianaceae</span></i><span style="font-family:Verdana;">), </span><i><span style="font-family:Verdana;">Gentiana</span></i> <i><span style="font-family:Verdana;">ciliolata</span></i> <span style="font-family:Verdana;">is</span><span style="font-family:Verdana;"> described and illustrated;it has a spectacular characteristics of calyx lobe ciliolate, distributed in Yunnan and Sichuan Provinces on the two sides of Jinsha River, and then two subspecies have been further re-classified as </span><i><span style="font-family:Verdana;">Gentiana</span></i> <i><span style="font-family:Verdana;">ciliolata</span></i> <span style="font-family:Verdana;">subsp</span><i><span style="font-family:Verdana;">.</span></i> <i><span style="font-family:Verdana;">ciliolata</span></i> <span style="font-family:Verdana;">and </span><i><span style="font-family:Verdana;">Gentiana</span></i> <i><span style="font-family:Verdana;">ciliolata</span></i> <span style="font-family:Verdana;">subsp.</span> <i><span style="font-family:Verdana;">longiloba</span></i> <span style="font-family:Verdana;">respectively</span><i><span style="font-family:Verdana;">.</span></i> <span style="font-family:Verdana;">The subsp.</span> <i><span style="font-family:Verdana;">ciliolata</span></i><span style="font-family:Verdana;"> idistributes</span> <span style="font-family:Verdana;">on the right side of Jinsha River in Yiliang County, Yunnan Province and the subsp</span><i><span style="font-family:Verdana;">.</span></i> <i><span style="font-family:Verdana;">longiloba</span></i><span style="font-family:Verdana;"> distributes itself </span><span style="font-family:Verdana;">on the southwest and west part of Sichuan Province, such as Leibo County, Wenchuan County and Mabian Yi Autonomous Prefacture, in the grassland under forests and moist rock slopes at an altitude of 1300 - 3200 m.
文摘Two new beta-dihydroagarofuran sesquiterpene polyol esters were isolated from Celastrus paniculatus.subsp.paniculatus. Their Structures were deduced on the basis of spectral analyses including 2D NMR. CO2 supercritical fluid was used in separation.
基金financial support provided by Benguet State University though the Office of the Vice-President for Research and Extension headed by Dr.Carlito P.Laurean。
文摘Coriaria,the only genus under family Coriariacea,receives a lot interest among botanist due to its controversial taxonomic treatment and unique phytochemistry that could be commercially important.The populations of Coriaria japonica subsp.intermedia in Cordillera Central Range(CCR),Philippines had never been studied in detail.With the need for more information on this controversial genus,the study deemed it necessary to present the distribution and morpho-anatomical description of C.japonica subsp.intermedia in CCR.A total of 123 populations were documented,found at an elevation range of 1334 to 2494 m asl and stretched between16°31’37.7"to 17°5’47.8"N and 120°39’40.3"to120°55’48"E.Mapping these populations showed a narrow linear pattern of distribution concentrated at the southern portion of CCR.The plant is a shrub with simple leaves,collateral buds,raceme inflorescence,bisexual flowers with persistent perianth forming an accessory fruit after fertilization.In terms of anatomy,much are of the usual dicot characters such as dorsiventral leaf with stomata concentrated in the lower epidermis,eustele with pith in the stem andpresence of secondary growth both on old stem and old root.Interesting findings of the study include the123 populations,the larger leaf measurements,longer petals and larger fruits than the original description given by Matsumura.Also,the paracytic type of stomata and presence of pith in the primary roots of C.japonica subsp.intermedia are interesting since these are common monocot characters while the plant is dicot.These findings are additional information that could help clear the controversies on the genus Coriaria.More importantly,our results could provide essential information for understanding the plant and for developing conservation strategies not just for this plant but also for the other related plants in the CCR biogeographical zone.
文摘Lactobacillus delbrueckii subsp. bulgaricus, which has been widely used as a fermented milk starter, is a type of probiotic, and certain strains are able to produce exopolysaccharide (EPS). EPS produced from L. bulgaricus contributes to the physical and biological function of dairy products by regulating immune response, and this tendency seems to place EPS with acidic groups. To date, six types of chemical structure have been determined and are basically composed from glucose (Glc), galactose (Gal), and rhamnose (Rha). Eps clusters on chromosome DNA control the EPS synthesis and are transcribed as one mRNA 14 genes with 18kb on L. bulgaricus Lfi5. Furthermore, L. bulgaricus is able to utilize lactose (Lac) as carbohydrate source, repeating units of EPS are synthesized from Glc 6-phosphate, generated by an Embden-Meyerhof (EM) pathway in cellular carbohydrate assimilation. This review discusses EPS produced from L. bulgaricus.
基金supported by the Science and Technology Project of Inner Mongolia, China (201502070)
文摘It is generally known that the culture for mycoplasma is time-consuming and a variety of nutrients are needed in the culture medium. This brings a lot of difficulties to mycoplasma research and application, including Mycoplasma mycoides subsp. capri(Mmc). Furthermore, little research on the characteristics of Mmc metabolism has been reported. In this study, Mmc PG3 strain cultures were investigated for dynamic gene expression. Culture samples were harvested during logarithmic phase(PG3-1), stationary phase(PG3-2), decline phase(PG3-3) and late decline phase(PG3-4). Twelve RNA samples(three replicates for each of the four growth stages considered) from these cultures were collected and sequenced. Paired comparison between consecutive growth phases in the four growth stages showed 45 significant differentially expressed genes(P〈0.01) were linked to PG3 metabolism. The enzymes these genes coded were mainly involved in ATP synthase, pyrimidine metabolism, nicotinate and nicotinamide metabolism, arginine and proline metabolism. Among these, cytidylate kinase, fructose 1,6-bisphosphate aldolases Class II, nicotinate-nucleotide adenylyltransferase and dihydrolipoamide dehydrogenase play a key role in Mmc metabolism. These results provide a baseline to build our understanding of the metabolic pathway of Mmc.