Screening of Substrate Formulas for Culture of Auricularia auricula Mycelia with Byproducts

[Objectives]This study was conducted to explore the source of substitutive substrate raw materials for cultivation of edible fungi,reduce the cutting and use of woody materials,and realize harmless treatment and resource utilization of byproducts from Chinese medicine production and bamboo processing.[Methods]Traditional Chinese medicine residue and bamboo shavings were used to partially replace hardwood sawdust in the conventional formula,and the growth of Auricularia auricula mycelia in large test tubes under different substrate formulas was studied.[Results]The results showed that the mycelia of A.auricula could grow normally on substrates with byproducts,and the mycelia grew differently with different formulas,and the performance of different strains of the same species was also different.Compared with the conventional formula,the suitable substitution amount of bamboo shavings for A.auricula strains was 10%-30%,and the substitution amount of Chinese medicine residue was 5%-15%.[Conclusions]This study provides reference for the efficient utilization of byproducts and the expansion of raw material sources for production of edible fungi.

Effects of Seed Soaking with Uniconazole and Substrate Formula on Seedling Quality of Rapeseed

[Objective] To cultivate blanket rapeseed seedlings suitable for automatic mechanical transplanting, the effects of seed soaking with uniconazole and substrate formula on seed germination and seedling quality of rapeseed were investigated using HY4 as the material. [Method] The rapeseed seeds were soaked in different concentrations（0, 5, 10, 20, 30, 40 and 50 mg/L） of uniconazole for 0, 1, 2, 4 and8 h, respectively. Subsequently, they were dried naturally. After the preliminary screening carried out in petri dishes and in a laboratory, the optimum soaking concentration and time of uniconazole were obtained. On the other hand, the rapeseed seeds were soaked in different concentrations（0, 5, 10, 15 and 20 mg/L） of uniconazole for 0, 1 and 2 h, respectively for outdoor pot experiment. At the same time, the screening of optimum substrate formula for cultivating blanket rapeseed seedlings was carried out. The field test was carried out to verify the screened optimum substrate formula. [Result] The results of petri dish experiment showed that seed soaking with uniconazole could delay seed germination, reduce seed germination rate, inhibit hypocotyl elongation and promote root growth. When the soaking concentration exceeded 30 mg/L and soaking time exceeded 2 h, the effect of seed soaking with uniconazole on seed germination was greater, and the seed soaking inhibited root growth. The results of outdoor pot experiment showed that the soaking concentrations and times all significantly reduced seedling height, slowed shoot growth and promoted root growth, which all contributed to the cultivation of blanket rapeseed seedlings suitable for automatic mechanical transplanting. In the substrate formula, the lower the proportion of soil was, the better the seedling growth was. In the substrate with lower proportion of soil, the seed germination rate, plant height,root vigor and dry matter accumulation were all increased（P〈0.05）, which was verified by the field test. When the soaking concentration was less than 10 mg/L and soaking time was less than 1 h, the uniconazole showed weaker regulation effect on seedling growth, and the rapeseed seedlings were higher. In the substrate with soil proportion less than 50%, the adequate fertility relatively weakened the regulation effect of uniconazole on seedling growth, and the rapeseed seedlings were larger. [Conclusion] When the soaking concentration ranged within 15-20 mg/L and soaking time ranged within 1-2 h and the proportion of soil in substrate was 50%,the height of cultivated rapeseed seedlings was less than 18 cm, there were 5-6leaves remained on each plant and the nursing period was shortened to 30-40 d,which were all beneficial to industrial production.