The deep_sea floor images are acquired under non_uniform illumination,the effects of which bring up many difficulties for image segmentation.The paper compares three methods of removing non_uniform illumination effect...The deep_sea floor images are acquired under non_uniform illumination,the effects of which bring up many difficulties for image segmentation.The paper compares three methods of removing non_uniform illumination effects.The effectiveness and robustness are evaluated on three test images with high_,moderate_ and low_density grains,respectively.The results show that local filtering algorithm does not remove the non_uniform illumination effects completely.The image subtracting algorithm will lose some of the dynamic range of the original data.The enhanced image stretching algorithm is the most effective one.展开更多
The fluorescence from the out-of-focus region excited by the sidelobes of a Bessel beam is the major concern for light-sheet fluorescence microscopy （LSFM） with Bessel beam plane illumination. Here, we propose a met...The fluorescence from the out-of-focus region excited by the sidelobes of a Bessel beam is the major concern for light-sheet fluorescence microscopy （LSFM） with Bessel beam plane illumination. Here, we propose a method of applying the subtractive imaging to overcome the limitation of the conventional LSFM with Bessel beam plane illumination. In the proposed method, the sample is imaged twice by line scanning using the extended solid Bessel beam and the ring-like Bessel beam. By subtracting between the two images with similar out-of-focus blur, the improved image quality with the suppression of the Bessel beam sidelobes and enhanced sectioning ability with improved contrast are demonstrated.展开更多
文摘The deep_sea floor images are acquired under non_uniform illumination,the effects of which bring up many difficulties for image segmentation.The paper compares three methods of removing non_uniform illumination effects.The effectiveness and robustness are evaluated on three test images with high_,moderate_ and low_density grains,respectively.The results show that local filtering algorithm does not remove the non_uniform illumination effects completely.The image subtracting algorithm will lose some of the dynamic range of the original data.The enhanced image stretching algorithm is the most effective one.
基金supported by the National Natural Science Foundation of China(Nos.61665006,61661028,61565012,and 61378062)the Natural Science Foundation of Jiangxi Province(Nos.20161BAB212041,20162BCB23012,and 20171ACB21018)
文摘The fluorescence from the out-of-focus region excited by the sidelobes of a Bessel beam is the major concern for light-sheet fluorescence microscopy （LSFM） with Bessel beam plane illumination. Here, we propose a method of applying the subtractive imaging to overcome the limitation of the conventional LSFM with Bessel beam plane illumination. In the proposed method, the sample is imaged twice by line scanning using the extended solid Bessel beam and the ring-like Bessel beam. By subtracting between the two images with similar out-of-focus blur, the improved image quality with the suppression of the Bessel beam sidelobes and enhanced sectioning ability with improved contrast are demonstrated.
