目的 探讨苏合香丸辅助治疗对老年脑卒中患者肺部感染肺功能和免疫功能的影响。方法 选取医院重症加强病房(Intensive care unit, ICU)2019年1月—2020年1月收治的重症脑卒中(Cerebralvascular accident, CVA)患者120例,根据治疗方法的...目的 探讨苏合香丸辅助治疗对老年脑卒中患者肺部感染肺功能和免疫功能的影响。方法 选取医院重症加强病房(Intensive care unit, ICU)2019年1月—2020年1月收治的重症脑卒中(Cerebralvascular accident, CVA)患者120例,根据治疗方法的不同分为观察组和对照组。对照组给予注射用盐酸头孢吡肟和胃管给复方甘草口服溶液,观察组在对照组基础上加用苏合香丸,均持续治疗14 d。统计并比较两组疗效,采用流式细胞仪测定外周血T淋巴细胞亚群,包括CD_(4)^(+)、CD_(8)^(+)T淋巴细胞和CD_(4)^(+)CD_(8)^(+)T淋巴细胞比值,经肺功能仪检测患者的肺功能指标[FEV1/FVC、 FEV1%pred、最大自主通气量(MVV)、血肌酐],记录并比较两组不良反应发生情况。结果 观察组治疗总有效率为100.00%(60/60),显著高于对照组的83.33%(50/60)(P<0.05)。观察组不良反应发生率为6.67%(4/60),显著低于对照组的11.67%(7/60)(P<0.05)。治疗后,观察组CD_(4)^(+)和CD_(4)^(+)CD_(8)^(+)水平改善效果显著优于对照组(P<0.05)。疗后,观察组FEV1/FVC、FEV1%pred和MVV高于对照组,血肌酐较低于对照组(P<0.05)。结论 苏合香丸辅助治疗对老年脑卒中患者肺部感染的疗效显著,快速缓解患者体征与临床症状,不良反应较少,可以显著改善患者机体的免疫功能、肺功能。展开更多
目的探讨针刺、苏合香丸联合高压氧对高血压脑出血(hypertensive intracerebral hemorrhage,HICH)患者的影响。方法随机选择2021年1月—2023年12月南京医科大学附属苏州医院神经康复科收治的60例HICH患者作为研究对象,通过信封法将研究...目的探讨针刺、苏合香丸联合高压氧对高血压脑出血(hypertensive intracerebral hemorrhage,HICH)患者的影响。方法随机选择2021年1月—2023年12月南京医科大学附属苏州医院神经康复科收治的60例HICH患者作为研究对象,通过信封法将研究对象分为研究组和对照组,各30例。对照组实施高压氧治疗,研究组在此基础上实施针刺、苏合香丸治疗。比较2组患者的治疗效果。结果干预后2组患者的美国国立卫生研究院卒中量表(National Institute of Health Stroke Scale,NIHSS)及Rivermead行为记忆评定量表(Rivermead behavioral memory test,RBMT)评分较干预前均有降低,且研究组低于对照组(P<0.05)。干预后2组患者的简易智力状态检查(mini-mental state examination,MMSE)、蒙特利尔认知评估(Montreal cognitive assessment,MoCA)、洛文斯顿认知功能评定测验(Loewenstein occupational therapy cognitive assessment,LOTCA)的水平较干预前均有改善,其中研究组的MMSE、Moca高于对照组,而LOTCA低于对照组(P<0.05)。干预后研究组的日常生活活动能力量表(activity of daily living scale,ADL)中工具性能力为(8.62±2.05)分,躯体生活评分为(7.62±1.38)分,均低于对照组的(11.24±2.28)分、(11.31±1.92)分(P<0.05)。结论对HICH患者实施针刺、苏合香丸联合高压氧可有效改善患者的神经功能、促进认知功能的恢复,从而提高治疗效果,改进日常生活能力。展开更多
The present study collected cerebrospinal fluid samples from the corpus striatum in rats treated with borneol, moschus, storax, and acorus tatarinowii using brain microdialysis technology. Levels of excitatory neurotr...The present study collected cerebrospinal fluid samples from the corpus striatum in rats treated with borneol, moschus, storax, and acorus tatarinowii using brain microdialysis technology. Levels of excitatory neurotransmitters aspartic acid and glutamate, as well as inhibitory neurotransmitters glycine and ^-aminobutyric acid, were measured in samples using reversed-phase high-performance liquid chromatography, phosphate gradient elution, and fluorescence detection. Results showed that concentrations of all four amino acid neurotransmitters significantly increased in the corpus striatum following treatment with borneol or moschus, but effects due to borneol were more significant than moschus. Acorus tatarinowii treatment increased ^-aminobutyric acid expression, but decreased glutamate concentrations. Storax increased aspartic acid concentrations and decreased glycine expression. Results demonstrated that borneol and moschus exhibited significant effects on con amino acid neurotransmitter expression; storax exhibited excitatory effects and acorus tatarinowii resulted in inhibitory effects.展开更多
Objective To explore the pharmacodynamic material basis and mechanism of action of volatile oil from Chuanxiong(Chuanxiong Rhizoma)-Suhexiang(Styrax)-Bingpian(Borneolum)(hereinafter referred to as C-S-B volatile oil)i...Objective To explore the pharmacodynamic material basis and mechanism of action of volatile oil from Chuanxiong(Chuanxiong Rhizoma)-Suhexiang(Styrax)-Bingpian(Borneolum)(hereinafter referred to as C-S-B volatile oil)in treating angina pectoris based on network pharmacology and to detect its protective effects against rat myocardial damage.Methods Gas chromatography-mass spectrometry(GC-MS)was used to determine the constituents of volatile oils from Chuanxiong(Chuanxiong Rhizoma),Suhexiang(Styrax),and Bingpian(Borneolum),and the targets of the three main constituents were found predicted and screened using the PharmMapper server,and Gene Cards and Coo LGe N databases.The STRING database and Cytoscape software were used to draw the protein-protein interaction(PPI)network,RStudio software was used to analyze Gene Ontology(GO)and Kyoto Encyclopedia of Genome and Genome(KEGG)pathways,and Cytoscape software was used to construct the component-target-pathwaydisease network.The rat model of myocardial injury was established by intraperitoneal injection of a large dose of isoprenaline hydrochloride.After continuous intervention with C-S-B volatile oil for 14 d,the ejection fraction(EF)and short axis shortening rate(FS)of the left ventricle were detected.The indices of myocardial damage were detected after hematoxylin-eosin(HE)staining.Results Fifteen volatile oil components from the C-S-B formula were identified.There are 470 targets of these volatile oil components and 401 angina-related genes.There are 28 core targets,including CHRM4,ADRA1 A,FGFR1,CHRM2,CYP2 A6,CHRM5,CHRM1,CHRM3,HDAC2,and MPO,etc..The results of the KEGG analysis indicated that the C-S-B formula probably interferes with the following pathways:neuroactive ligand-receptor interactions,calcium signaling,cytochrome P450 metabolism of heteropoietin,among others.The results of animal experiments showed that the C-S-B formula essential oil could significantly improve the following myocardial indices in rats with myocardial injury:EF,FS,left ventricular end-systolic diameter(LVIDs),left ventricular end-diastolic diameter(LVIDd),and stroke volume(SV),and all the differences were statistically significant(P<0.01).Conclusion The mechanism of action of volatile oil components in the C-S-B formula in treating angina pectoris was analyzed using multi-component,multi-target and multi-pathway systems,which has laid a foundation for further revealing its mechanism of action.Animal experiments have shown that the volatile oil of the C-S-B formula can improve EF,FS,and other indices of myocardial damage in a rat model,thus relieving myocardial damage caused by heart hyperactivity,improving cardiac function,and protecting against myocardial damage.展开更多
文摘目的探讨针刺、苏合香丸联合高压氧对高血压脑出血(hypertensive intracerebral hemorrhage,HICH)患者的影响。方法随机选择2021年1月—2023年12月南京医科大学附属苏州医院神经康复科收治的60例HICH患者作为研究对象,通过信封法将研究对象分为研究组和对照组,各30例。对照组实施高压氧治疗,研究组在此基础上实施针刺、苏合香丸治疗。比较2组患者的治疗效果。结果干预后2组患者的美国国立卫生研究院卒中量表(National Institute of Health Stroke Scale,NIHSS)及Rivermead行为记忆评定量表(Rivermead behavioral memory test,RBMT)评分较干预前均有降低,且研究组低于对照组(P<0.05)。干预后2组患者的简易智力状态检查(mini-mental state examination,MMSE)、蒙特利尔认知评估(Montreal cognitive assessment,MoCA)、洛文斯顿认知功能评定测验(Loewenstein occupational therapy cognitive assessment,LOTCA)的水平较干预前均有改善,其中研究组的MMSE、Moca高于对照组,而LOTCA低于对照组(P<0.05)。干预后研究组的日常生活活动能力量表(activity of daily living scale,ADL)中工具性能力为(8.62±2.05)分,躯体生活评分为(7.62±1.38)分,均低于对照组的(11.24±2.28)分、(11.31±1.92)分(P<0.05)。结论对HICH患者实施针刺、苏合香丸联合高压氧可有效改善患者的神经功能、促进认知功能的恢复,从而提高治疗效果,改进日常生活能力。
基金supported by the National Natural Science Foundation of China, No. 81173572
文摘The present study collected cerebrospinal fluid samples from the corpus striatum in rats treated with borneol, moschus, storax, and acorus tatarinowii using brain microdialysis technology. Levels of excitatory neurotransmitters aspartic acid and glutamate, as well as inhibitory neurotransmitters glycine and ^-aminobutyric acid, were measured in samples using reversed-phase high-performance liquid chromatography, phosphate gradient elution, and fluorescence detection. Results showed that concentrations of all four amino acid neurotransmitters significantly increased in the corpus striatum following treatment with borneol or moschus, but effects due to borneol were more significant than moschus. Acorus tatarinowii treatment increased ^-aminobutyric acid expression, but decreased glutamate concentrations. Storax increased aspartic acid concentrations and decreased glycine expression. Results demonstrated that borneol and moschus exhibited significant effects on con amino acid neurotransmitter expression; storax exhibited excitatory effects and acorus tatarinowii resulted in inhibitory effects.
基金funding support from the Major Science and Technology Research and Development Special Project of Jiangxi Science and Technology Department(No.20194ABC28009)National Key Research and Development Plan(No.2018YFC1706404)。
文摘Objective To explore the pharmacodynamic material basis and mechanism of action of volatile oil from Chuanxiong(Chuanxiong Rhizoma)-Suhexiang(Styrax)-Bingpian(Borneolum)(hereinafter referred to as C-S-B volatile oil)in treating angina pectoris based on network pharmacology and to detect its protective effects against rat myocardial damage.Methods Gas chromatography-mass spectrometry(GC-MS)was used to determine the constituents of volatile oils from Chuanxiong(Chuanxiong Rhizoma),Suhexiang(Styrax),and Bingpian(Borneolum),and the targets of the three main constituents were found predicted and screened using the PharmMapper server,and Gene Cards and Coo LGe N databases.The STRING database and Cytoscape software were used to draw the protein-protein interaction(PPI)network,RStudio software was used to analyze Gene Ontology(GO)and Kyoto Encyclopedia of Genome and Genome(KEGG)pathways,and Cytoscape software was used to construct the component-target-pathwaydisease network.The rat model of myocardial injury was established by intraperitoneal injection of a large dose of isoprenaline hydrochloride.After continuous intervention with C-S-B volatile oil for 14 d,the ejection fraction(EF)and short axis shortening rate(FS)of the left ventricle were detected.The indices of myocardial damage were detected after hematoxylin-eosin(HE)staining.Results Fifteen volatile oil components from the C-S-B formula were identified.There are 470 targets of these volatile oil components and 401 angina-related genes.There are 28 core targets,including CHRM4,ADRA1 A,FGFR1,CHRM2,CYP2 A6,CHRM5,CHRM1,CHRM3,HDAC2,and MPO,etc..The results of the KEGG analysis indicated that the C-S-B formula probably interferes with the following pathways:neuroactive ligand-receptor interactions,calcium signaling,cytochrome P450 metabolism of heteropoietin,among others.The results of animal experiments showed that the C-S-B formula essential oil could significantly improve the following myocardial indices in rats with myocardial injury:EF,FS,left ventricular end-systolic diameter(LVIDs),left ventricular end-diastolic diameter(LVIDd),and stroke volume(SV),and all the differences were statistically significant(P<0.01).Conclusion The mechanism of action of volatile oil components in the C-S-B formula in treating angina pectoris was analyzed using multi-component,multi-target and multi-pathway systems,which has laid a foundation for further revealing its mechanism of action.Animal experiments have shown that the volatile oil of the C-S-B formula can improve EF,FS,and other indices of myocardial damage in a rat model,thus relieving myocardial damage caused by heart hyperactivity,improving cardiac function,and protecting against myocardial damage.
