The present study is to determine the suitable protoscolices (PSCs) density for long-time culturing in vitro. The PSCs were divided into eight groups with different densities and the viability tests were carried out...The present study is to determine the suitable protoscolices (PSCs) density for long-time culturing in vitro. The PSCs were divided into eight groups with different densities and the viability tests were carried out with 0.1% methylene blue staining. Then the infection ability of cultured PSCs was assessed by the mean cyst weight of mice inoculated intraperitoneally with PSCs after 8 months post-infection.展开更多
基金supported by International Collaboration on Drug and Diagnostics Innovation of Tropical Diseases in China (International S&T Cooperation 2010DFB73280)Technology Development Research for Science Research Institute (2011EG150312)the Youth Project of Shanghai Municipal Health Bureau (87)
文摘The present study is to determine the suitable protoscolices (PSCs) density for long-time culturing in vitro. The PSCs were divided into eight groups with different densities and the viability tests were carried out with 0.1% methylene blue staining. Then the infection ability of cultured PSCs was assessed by the mean cyst weight of mice inoculated intraperitoneally with PSCs after 8 months post-infection.