Understanding bacterial adsorption and the evolution of biofilms on arsenopyrite with different surface structures is of great signific-ance to clarifying the mechanism of microbe-mineral interfacial interactions and ...Understanding bacterial adsorption and the evolution of biofilms on arsenopyrite with different surface structures is of great signific-ance to clarifying the mechanism of microbe-mineral interfacial interactions and the production of acidic mine drainage impacting the environ-ment.In this study,the attachment of Sulfobacillus thermosulfidooxidans cells and subsequent biofilm formation on arsenopyrite with different surface structures in the presence of dissolved As(Ⅲ)was studied.Arsenopyrite slices with a specific surface were obtained by electrochemic-al corrosion at 0.26 V.The scanning electronic microscopy-energy dispersion spectra analyses indicated that the arsenopyrite surface deficient in sulfur and iron obtained by electrochemical treatment was not favorable for the initial adsorption of bacteria,and the addition of As(Ⅲ)in-hibited the adsorption of microbial cells.Epifluorescence microscopy showed that the number of cells attaching to the arsenopyrite surface in-creased with time;however,biofilm formation was delayed significantly when As(Ⅲ)was added.展开更多
基金Projects(31470230,51320105006,51604308)supported by the National Natural Science Foundation of ChinaProject(2017RS3003)supported by the Youth Talent Foundation of Hunan Province of China+1 种基金Project(2018JJ2486)supported by the Natural Science Foundation of Hunan Province of ChinaProject(2018WK2012)supported by the Key Research and Development Projects in Hunan Province,China。
基金This work was financially supported by National Natural Science Foundation of China(Nos.51774342,41802038,U1608254,51861135305,and 41830318)Beijing Syn-chrotron Radiation Facility Public User Program(2018-BEPC-PT-002240).
文摘Understanding bacterial adsorption and the evolution of biofilms on arsenopyrite with different surface structures is of great signific-ance to clarifying the mechanism of microbe-mineral interfacial interactions and the production of acidic mine drainage impacting the environ-ment.In this study,the attachment of Sulfobacillus thermosulfidooxidans cells and subsequent biofilm formation on arsenopyrite with different surface structures in the presence of dissolved As(Ⅲ)was studied.Arsenopyrite slices with a specific surface were obtained by electrochemic-al corrosion at 0.26 V.The scanning electronic microscopy-energy dispersion spectra analyses indicated that the arsenopyrite surface deficient in sulfur and iron obtained by electrochemical treatment was not favorable for the initial adsorption of bacteria,and the addition of As(Ⅲ)in-hibited the adsorption of microbial cells.Epifluorescence microscopy showed that the number of cells attaching to the arsenopyrite surface in-creased with time;however,biofilm formation was delayed significantly when As(Ⅲ)was added.
