Coherent anti-Stokes Raman scattering(CA RS)microscopy can resolve the chemical compo-nents and distribution of living biological systems in a label-firee manner and is favored in several disciplines.Current CA RS mic...Coherent anti-Stokes Raman scattering(CA RS)microscopy can resolve the chemical compo-nents and distribution of living biological systems in a label-firee manner and is favored in several disciplines.Current CA RS microscopes typically use bulky,high-performance solid-state lasers,which are expensive and sensitive to environmental changes.With their relatively low cost and environmental sensitivity,supercontinum fiber(SF)lasers with a small footprint have found increasing use in biomedical applications.Upon these features,in this paper,we homebuilt a low-cost CARS microscope based on a SF laser module(scCA RS microscope).This SF laser module is specially customized by adding a time synchronized seed source channel to the SF laser to form a dual-channel output laser.The performance of the scCARS microscope is evaluated with dimethyl sulfoxide,whose results confirm a spatial resolution of better than 500nm and a detection sensitivity of millimolar concentrations.The dual-color imaging capability is further demonstrated by imaging different species of mixed microspheres.We finally explore the potential of our scCARS microscope by mapping lipid droplets in different cancer cells and corneal stromal lenses.展开更多
Fiber-based mid-infrared(MIR)supercontinuum(SC)sources benefit from their spectral brightness and spatial coherence that are needed for many applications,such as spectroscopy and metrology.In this paper,an SC spanning...Fiber-based mid-infrared(MIR)supercontinuum(SC)sources benefit from their spectral brightness and spatial coherence that are needed for many applications,such as spectroscopy and metrology.In this paper,an SC spanning from 2μm to 6μm is demonstrated in cascaded ZrF4-BaF2-LaF3-AlF3-NaF(ZBLAN)and As2Se3 step-index fibers.The pump source is a ZBLAN fiber-based MIR SC laser with abundant high-peak-power soliton pulses between 3000 nm and 4200 nm.By concatenating the ZBLAN fiber and the As2 Se3 fiber,efficient cascading red-shifts are obtained in the normal dispersion region of the As2 Se3 fiber.The spectral behavior of cascaded SC generation shows that the long-wavelength proportion of MIR SC generated in the ZBLAN fiber plays a critical role for further spectral extension in the As2 Se3 fiber.展开更多
基金This work was supported in part by the National Key R&D Program of China(2018YFC0910600)the National Natural Science Foundation of China(81871397)+4 种基金the National Young Talent Program,the Shaanxi Science Fund for Distinguished Young Scholars(2020JC-27)the Key Research and Development Program of Shaanxi(2021ZDLSF04-05)the Shaanxi Young Top-notch Talent Program,the Best Funded Projects for the Scientific and Technological Activities for Excellent Overseas Researchers in Shaanxi Province(2017017)the Fundamental Research Funds for Central Universities(QTZX2105)Xueli Chen would like to thank Dr.Chi Zhang at Purdue University for his help in building the CARS microscope.
文摘Coherent anti-Stokes Raman scattering(CA RS)microscopy can resolve the chemical compo-nents and distribution of living biological systems in a label-firee manner and is favored in several disciplines.Current CA RS microscopes typically use bulky,high-performance solid-state lasers,which are expensive and sensitive to environmental changes.With their relatively low cost and environmental sensitivity,supercontinum fiber(SF)lasers with a small footprint have found increasing use in biomedical applications.Upon these features,in this paper,we homebuilt a low-cost CARS microscope based on a SF laser module(scCA RS microscope).This SF laser module is specially customized by adding a time synchronized seed source channel to the SF laser to form a dual-channel output laser.The performance of the scCARS microscope is evaluated with dimethyl sulfoxide,whose results confirm a spatial resolution of better than 500nm and a detection sensitivity of millimolar concentrations.The dual-color imaging capability is further demonstrated by imaging different species of mixed microspheres.We finally explore the potential of our scCARS microscope by mapping lipid droplets in different cancer cells and corneal stromal lenses.
基金National Natural Science Foundation of China(Nos.52001140,52274363)Guangdong Basic Applied Basic Research Foundation,China(Nos.2022A1515010558,2022A1515011597,2022A1515240065)。
基金Project supported by the National Natural Science Foundation of China(Grant Nos.61435009,61235008,and 61405254)the Fund from China Scholarship Council(Grant No.201803170210)Hunan Provincial Innovation Foundation for Postgraduate(Grant No.CX2018B008)
文摘Fiber-based mid-infrared(MIR)supercontinuum(SC)sources benefit from their spectral brightness and spatial coherence that are needed for many applications,such as spectroscopy and metrology.In this paper,an SC spanning from 2μm to 6μm is demonstrated in cascaded ZrF4-BaF2-LaF3-AlF3-NaF(ZBLAN)and As2Se3 step-index fibers.The pump source is a ZBLAN fiber-based MIR SC laser with abundant high-peak-power soliton pulses between 3000 nm and 4200 nm.By concatenating the ZBLAN fiber and the As2 Se3 fiber,efficient cascading red-shifts are obtained in the normal dispersion region of the As2 Se3 fiber.The spectral behavior of cascaded SC generation shows that the long-wavelength proportion of MIR SC generated in the ZBLAN fiber plays a critical role for further spectral extension in the As2 Se3 fiber.
基金the project of the Czech Science Foundation (No.20-19170S)the German Research Foundation (Deutsche Forschungsgemeinschaft (DFG))for financial support within the scope of project (No.SCHA 1484/46-1).
