Chinese Sapium sebiferum(L.)Roxb has great potential to emerge as a promising biodiesel resource plant due to abundant oil content in seed.However,the unavailability of transcriptomic and genomic data impedes the prog...Chinese Sapium sebiferum(L.)Roxb has great potential to emerge as a promising biodiesel resource plant due to abundant oil content in seed.However,the unavailability of transcriptomic and genomic data impedes the progress of application in biodiesel production.Transcriptome analysis will help to further exploit tallow and kernel oil utilization in industry and facilitate molecular-breeding-based genetic improvement of S.sebiferum.Illumina hiseq 2000 sequencing platform was used to perform de novo transcriptome assembly and gene expression analyses of seeds from three strains in three developmental stages.A total of 188,254 unigenes over 200 bp were assembled with average length of 699 bp.These transcriptic unigenes were functionally assigned by GO and KEGG pathways.RPKM was used to identify differentially expressed unigenes of five cDNA libraries.Transcriptic genes endcoding enzymes related to fatty acid metabolism were evaluated.qRT-PCR was employed to confirm five key enzymes involved in fatty acid and triacylglycerol biosynthesis.This study provided comprehensive gene expression information of Chinese S.sebiferum seed at transcriptional level.These transcriptome data will facilitate high-oil-content genetic cultivation of S.sebiferum,which will further exploit tallow and kernel oil utilization in biodiesel industry.展开更多
基金funded by the National Science&Technology Pillar Program during the Twelfth Five-year Plan Period of China(2011BAD22B08).
文摘Chinese Sapium sebiferum(L.)Roxb has great potential to emerge as a promising biodiesel resource plant due to abundant oil content in seed.However,the unavailability of transcriptomic and genomic data impedes the progress of application in biodiesel production.Transcriptome analysis will help to further exploit tallow and kernel oil utilization in industry and facilitate molecular-breeding-based genetic improvement of S.sebiferum.Illumina hiseq 2000 sequencing platform was used to perform de novo transcriptome assembly and gene expression analyses of seeds from three strains in three developmental stages.A total of 188,254 unigenes over 200 bp were assembled with average length of 699 bp.These transcriptic unigenes were functionally assigned by GO and KEGG pathways.RPKM was used to identify differentially expressed unigenes of five cDNA libraries.Transcriptic genes endcoding enzymes related to fatty acid metabolism were evaluated.qRT-PCR was employed to confirm five key enzymes involved in fatty acid and triacylglycerol biosynthesis.This study provided comprehensive gene expression information of Chinese S.sebiferum seed at transcriptional level.These transcriptome data will facilitate high-oil-content genetic cultivation of S.sebiferum,which will further exploit tallow and kernel oil utilization in biodiesel industry.
文摘采用胰脂酶分解的方法对几种乌桕[Sapium sebiferum(L.)Roxb.]种子桕脂甘油三酯组成进行了测定,桕脂的甘油三酯是由9—14种甘油三酯所组成。与可可脂相似之处是甘油三酯2-位上的脂肪酸,82—88%为油酸。二饱和酸甘油酯占81—88%,其中以 POP 为主(74—81%);单饱和酸甘油酯占1.8—6.0%;而三饱和酸甘油酯占9.6—15.6%,主要是 PPP(8.5—14.0%)。桕脂经过适当的加工处理,可以做可可脂的代用品。将湖南产的乌桕与广西栽培的4个品种(“大粒鸡爪”、“蜈蚣”、“铜锤”和“小粒鸡爪”)桕脂中的甘油三酯组成比较,以“大粒鸡爪”桕脂中二饱和酸甘油酯的比例最高(88.0%),三饱和酸甘油酯的含量最低(9.6%)。
