Effect of Chinese Herbs for Activating Blood Circulation,Removing Stasis and Supplementing Qi on the Circulating Endothelial Cells in Patients with Unstable Angina Pectoris

Objective: To observe the effect and clinical significance of circulating endothelial cells (CEC) in the pathogenesis of coronary heart disease with unstable angina pectoris (CHD-UAP), and to explore the protective effect of Chinese herbs for activating blood circulation, removing stasis and supplementing Qi (CH) on CHD-UAP patient's CEC. Methods: Sixty patients with diagnosis of CHD-UAP confirmed and differentiated to be Qi-deficiency and blood stasis by TCM were randomly divided into two groups and treated, on the basis of Western drug-therapy, with Tongxinluo capsule (通心络胶囊, TXL) and Huoxue Tongmai capsule (活血通脉胶囊, HXTM) respectively by way of oral taking three times a day, 3 capsules every time, with 1 month as one therapeutic course. The number of CEC in patients' blood circulation was counted before and after treatment. Besides, the number of CEC in 30 healthy persons was also counted for control. Results: The number of CEC in CHD-UAP patients was significantly higher than that in the healthy persons (P<0.01). After the patients were treated with CH, either TXL or HXTM, it significantly decreased (P<0.01)with insignificant difference between the two treated groups. Conclusion: CEC in CHD-UAP patients is severely damaged and endothelial function in disorder, Chinese herbs have protective effect on patients' CEC.

Effect of Chinese Drugs for Supplementing Qi,Nourishing Yin and Activating Blood Circulation on Myocardial Perfusion in Patients with Acute Myocardial Infarction after Revascularization

Objective:To observe the effects of Chinese drugs for supplementing qi,nourishing yin and activating blood circulation on the myocardial perfusion in acute myocardial infarction(AMI) patients after revascularization.Methods:Eighty patients with anterior or inferior ventricular wall AMI,who had received revascularization by intravenous thrombolysis or coronary bypass,were randomized into the treated group and the control group equally,both treated with conventional Western medical treatment,but combined,resp...

Protective Effect of Chinese Herbs for Supplementing Qi,Nourishing Yin and Activating Blood Circulation on Heart Function of Patients with Acute Coronary Syndrome after Percutaneous Coronary Intervention

Objective： To investigate the effects of Chinese herbs for supplementing qi, nourishing yin and activating blood circulation on heart function of patients with acute coronary syndrome （ACS） after successful percutaneous coronary intervention （PCI）. Methods： One hundred patients with ACS after successful PCI were randomly assigned to a Western medicine （WM） treatment group （WMG） and a combined treatment group （CMG） treated by Chinese herbs for supplementing qi, nourishing yin and activating blood circulation, besides Western medicine treatment, with 50 cases in each group. Both treatment courses were 6 months. The follow- up was scheduled at baseline, 6 months and 1 year after PCI, and New York Heart Association （NYHA） functional class, Chinese medicine （CM） symptom scores, blood stasis syndrome scores, and major adverse cardiovascular events （MACE） were observed, serum levels of N-terminal pro-brain natriuretic peptide （NT-proBNP） and hyper-sensitivity C-reactive protein （Hs-CRP） were measured, an echocardiogram was conducted to examine left ventricular ejection fraction （LVEF）, left ventricular end-diastolic volume （LVEDV）, left ventricular end-systolic volume （LVESV）, inter-ventricular septal thickness （IVST）, left ventricular posterior wall thickness （LVPWT）, and ventricular wall motion index （VWMI）. Results： Compared with the baseline, LVEF significantly increased （P〈0.01）, and CM symptom scores, blood stasis syndrome scores, VWMI, LVEDV, LVESV, NT-proBNP, and Hs-CRP all decreased （P〈0.01） in both groups at 6 months and at 1 year after PCI. There were no significant differences in all the above parameters at 1 year vs those at 6 months after PCI （P〉0.05）. VWMI, LVEDV, LVESV, NT-proBNP, Hs-CRP, LVEF, and CM symptom and blood stasis syndrome scores were all improved obviously in CMG than those in WMG （P〈0.05 or P〈0.01） at 6 months and at 1 year after PCI. There were no significant differences in NYHA functional class between CMG and WMG at different follow-up timepoints; it was notable that P value was 0.054 when comparing the cases of NYHA functional class between the two groups at 1-year follow-up. During the 1-year follow-up, 3 MACE and 11 MACE occurred in CMG and WMG, respectively; the MACE rate in CMG was lower than that in WMG （6% vs 22%, P〈0.05）. Conclusion： Chinese herbs for supplementing qi, nourishing yin and activating blood circulation could improve heart function, reduce the CM symptom scores and blood stasis syndrome scores, and decrease the incidence of MACE in patients with ACS after successful PCI.

Supplementing Qi and activating blood circulation method to treat vertebrobasilar dolichoectasia with posterior circulatory watershed infarction:a case report of two patients

Vertebrobasilar dolichoectasia(VBD),a rare posterior circulation vascular variant disease,is an important risk factor for many acute cerebrovascular diseases.An insufficient understanding of VBD often leads to misdiagnose.Two cases of VBD that were initially diagnosed as posterior circulation watershed infarction are reported here.Absence of common causes of stroke including hypoperfusion,blood system diseases,carotid and aortic dissection,and eosinophil elevation,the symptoms of the 2 patients met the diagnostic criteria of VBD.Both patients displayed symptoms that were in line with the Traditional Chinese Medicine(TCM)syndrome pattern of"Qi deficiency and blood stasis".Accordingly,they were comprehensively treated with Supplementing Qi and activating blood circulation method.The clinical manifestations of the 2 patients were remarkably improved and no recurrence of watershed infarction was found in a 1-year follow-up.A detailed medical history and laboratory examination are capable of improving diagnostic accuracy of VBD.TCM treatment based on syndrome identification might be a promising candidate for VBD management.

Clinical Study on Effect of Chinese Herbal Medicine for Supplementing Kidney and Qi and Activating Blood Circulation in Treating Intrauterine Growth Retardation of Fetus

Objective: To explore the therapeutic effect and the possible working mechanism in using Chinese herbal medicine (CHM) for supplementing Kidney and Qi, and activating blood circulation in treating intrauterine growth retardation of fetus (IUGR). Methods: Fifty-five cases of IUGR were divided into two groups, 30 cases in the CHM group treated with CHM and the 25 in the control group treated with amino acids. The effect of CHM treatment was observed and compared with that of the control group, normal pregnancy group and non-treated IUGR group. Results: Body weight of the newborns in the CHM was markedly higher than that in the control group. Not only the maternal fundal height (FH) and the abdominal circumference (AC), but also the fetal growth parameters, including biparietal diameter, head circumference (HC), and femur length (FL) in the CHM group increased much faster than those in the control group. After CHM treatment, the maternal serum levels of estriol (E3) and human placental lactogen (hPL) approached to those in the normal pregnancy group, but the control group,in comparison with the normal pregnancy group, was significantly different. The umbilical venous plasma concentration of essential amino acids in both treated groups improved, but the improvement in the CHM group was more significant than that in the control group. No apparent adverse effect of CHM was observed in either mother or fetus.Conclusion: CHM for supplementing Kidney and Qi and activating blood circulation was more effective in improving placental function and enhancing amino acid transportation than amino acid

益气活血方药治疗原发性肝癌的免疫学机制概述

归纳总结具有益气和/或活血功效的中药单体、单味中药以及中药复方治疗原发性肝癌的免疫学机制,包括对T淋巴细胞亚群、巨噬细胞、髓源性抑制细胞(myeloid-derived suppressor cell,MDSC)、肿瘤细胞表面受体、细胞因子以及红细胞免疫在数量和功能上的调节,阐述其抗肿瘤作用,为进一步的基础研究和临床应用提供思路。

益气活血中药治疗慢性心力衰竭机制的研究进展

慢性心力衰竭是一种进行性的、使患者身体衰弱的疾病,病死率和再住院率居高不下。据报道,我国慢性心力衰竭患者基数庞大且持续增加,已成为严重的社会公共卫生问题。近年来,虽然现代医学在药物治疗方面不断改进,但是仍无法克服其致死率高、不良反应明显等不足。中医学认为慢性心力衰竭病位在心,心气亏虚,推动无力,从而导致血脉瘀滞。因此,益气活血法是治疗慢性心力衰竭的有效方法。益气活血药是以补虚扶弱、疏通血脉为主要功效,以治疗气虚血瘀证为主的一类中药。其代表药物有人参、黄芪、丹参、当归、川芎等,是治疗慢性心力衰竭心气虚证的常用药物。人参、黄芪益气强心,配合当归、川芎活血化瘀,在缓解心气不足、心血不运中显示出了良好疗效。诸多研究表明益气活血类中药具有多成分、多途径、多靶点的特点,在改善慢性心力衰竭患者症状,提高生活质量方面具有独特优势。近期研究发现其作用机制与血流动力学、神经内分泌系统、内皮损伤、免疫调节、炎症因子、氧化应激、心肌能量代谢、心室重构、自噬和凋亡等机制有关。文章旨在总结益气活血类中药改善心功能的作用机制,以期为慢性心力衰竭气虚血瘀证的治疗提供新思路。

基于数据挖掘及网络药理学的益气活血法治疗膝骨关节炎的机制研究

目的:基于数据挖掘分析益气活血法的高频配伍规律,并通过网络药理学方法研究其核心药物组治疗膝骨关节炎的作用机制。方法:应用Python 3.8.1编程对中国中医药在线数据库进行智能抓取,分析其配伍规律,以符合支持度≥0.30,置信度≥0.90,提升度≥1.00的中药为核心药物组,通过中药系统药理学数据库与分析平台(TCMSP)获取核心药物组的主要活性成分,利用PubChem平台、Swiss Target Prediction平台收集药物靶标基因,通过GeneCards及OMIM数据库获得膝骨关节炎相关靶基因,利用Venny数据库获取药物靶点及疾病靶点的交集,采用Cysoscape软件和STRING数据库分析蛋白质-蛋白质相互作用网络,借助DAVID软件对关键靶基因进行基因本体论(GO)分析和京都基因与基因组百科全书(KEGG)通路分析,通过Systems Dock Web Site对成分与关键靶标基因进行分子对接验证。结果:以益气活血法中使用频次最高的药对黄芪-当归为核心药物组,并筛选得到22个活性成分,涉及74个关键靶基因。网络分析结果表明,核心药物组治疗膝骨关节炎的关键靶点为肿瘤坏死因子(Tumor Necrosis Factor,TNF)、Jun原癌基因(Jun Proto-Oncogene,JUN)、白细胞介素(Interleukin,IL)-6、IL-4、IL-1β和趋化因子2(C-X-C Motif Chemokine Ligand 2,CXCL2)等;GO富集分析主要涉及脂多糖反应、细胞死亡、迁移的正向调节、细胞因子受体结合等生物学过程,KEGG通路中晚期糖基化终末产物(Advanced Glycation End-product,AGE)与其受体(Receptor for Advanced Glycation End Products,RAGE)信号通路、TNF信号通路、IL-17信号通路与治疗膝骨关节炎密切相关,分子对接显示关键靶点与山柰酚、β-谷甾醇、槲皮苷具有较高亲和力。结论:初步预测了益气活血法核心药物组对膝骨关节炎的基本药理基础及作用机制,益气活血法治疗膝骨关节炎具有多基因参与、多信号通路的特点。

益气活血法在糖尿病周围神经病变中的应用进展

糖尿病周围神经病变(Diabetic Peripheral Neuropathy,DPN)是糖尿病(Diabetes mellitus,DM)持续发展所引起的常见慢性并发症之一。研究报道,50%~66%的DM患者最终均会出现该病变。DPN主要包括感觉神经、运动神经及自主神经系统的病变,其标志性临床特征是大、小神经纤维的进行性损伤。DPN患病率高、致病广泛、致残率高,给家庭及社会造成沉重负担。其所造成的反复肢体感染、溃疡甚至截肢是导致DM患者死亡和残疾的主要原因。目前,西医治疗以控制血糖、缓解氧化应激、改善微循环等疗法为主,在改善患者临床症状、控制疾病进展等方面效果有限。中医治疗本病历史悠久,认为本病病机多以气虚为本,瘀血为标,呈现虚实夹杂的病机特征。纵观文献,笔者发现诸医家论治本病多从健脾益气,活血通络入手,以黄芪桂枝五物汤、补阳还五汤等经典方剂加减论治,标本兼治,消补兼施,并以益气活血法为主,配合针刺、足浴等中医外治法实现多靶点、多途径防治本病,在改善患者临床症状、提高神经传导速度、减轻炎症反应、改善神经营养状态等方面疗效确切而显著,兹将近年益气活血法指导下的相关中医内治法包括中药汤剂、中成药及外治法在DPN中的应用进展综述如下。

通冠胶囊对冠心病介入术后气虚血瘀证患者的影响

目的观察益气活血中药通冠胶囊对经皮冠状动脉介入治疗(percutaneous coronary intervention,PCI)后气虚血瘀证患者的影响。方法将100例成功施行PCI患者分为两组,每组50例。对照组手术前、中、后选用抗栓、抗凝等西药常规治疗。治疗组在对照组治疗基础上加用通冠胶囊,两组疗程均为1个月。于术前1天、术后3天和术后30天3个时间点观察以下项目:心绞痛疗效、主要中医证候、气虚证评分和血瘀证评分。结果治疗后治疗组心绞痛疗效96.0%(48/50例),对照组为92.0%(46/50例),组间差异无统计学意义(P>0.05)。PCI术后3天两组气虚证积分较术前有增加趋势,术后30天对照组较术前明显升高;术后3、30天两组血瘀证积分较术前明显降低(P<0.05)。治疗组术后30天气虚证积分和血瘀证积分较对照组均明显降低(P<0.05)。结论益气活血中药通冠胶囊能显著改善PCI术后患者气虚血瘀证表现。

心肌梗死后心力衰竭大鼠心脏基因表达谱及活血益气方药对其心脏的影响

目的观察活血益气方药对心肌梗死后心力衰竭模型大鼠心脏功能结构的影响。方法以大鼠左冠状动脉结扎术造成心肌梗死后心衰模型。对心衰形成期 (术后 10天 )、稳定期 (术后 8周 )大鼠左心室梗死区、非梗死区和同期假手术组左心室分别取材并提取总RNA ,以 6张大鼠 4 0S基因芯片 (40 96个基因 /张芯片 )对其进行检测 ;用基因芯片分析软件Genespring、Treeview、Clustering、SOM对芯片结果进行分析。术后 4周以活血益气方药对模型大鼠开始治疗 ,共 4周 ,以卡托普利作为阳性对照药 ;经阻抗法观察给药前后大鼠心脏功能和心脏系数的变化。结果 (1)用Genespring软件分析基因表达谱芯片检测结果发现有参与能量代谢、心肌细胞骨架及纤维化等 13类共千余条表达显著上调和下调的基因 ,在梗死区差异表达基因以形成期最多 (10 86条 )、稳定期次之 (72 4条 ) ;而非梗死区差异表达基因也以形成期最多 (196条 ) ,稳定期较少 (97条 )。 (2 )心肌梗死后心衰大鼠经活血益气方药、卡托普利治疗 ,心功能明显改善 ,每搏输出量 (SV)、心输出量 (CO)、心脏指数 (CI)均较治疗前显著提高 (P <0 0 1) ;同时活血益气方药还能改善模型动物的心脏系数 (与模型组比较 ,P <0 0 1,与假手术组比较 ,P >0 0 5 )。

早期应用活血和益气中药抑制心衰大鼠左室重构和凋亡的对比研究

目的:比较早期应用活血、益气中药,抑制心梗后心衰大鼠左室重构和心肌细胞凋亡的作用特点。方法:结扎大鼠左冠状动脉致心梗后心力衰竭,随机分为模型组、活血组(8 g.kg-1)、益气组(8 g.kg-1)、气血组(16g.kg-1)、西药组(卡托普利10.125 mg.kg-1),另外有假手术组作为对照。于大鼠心梗后次日给药、疗程分别为4,8周。采用阻抗法测定心功能;病理染色图象分析测定左室腔周长和面积、单位面积内心肌细胞核数、单位面积内胶原的含量;TUNEL法测定心肌细胞凋亡百分率以及放射免疫法测定心肌组织中血管紧张素Ⅱ(AngⅡ)的含量。结果:各治疗组都改善了心衰大鼠左室收缩功能、减小左室腔面积、抑制胶原增殖、AngⅡ含量和心肌细胞凋亡,与模型组比较有显著差异;活血组给药8周改善心脏指数(C I)、减小左室腔面积、降低心肌AngⅡ的结果与假手术组已无显著性区别;活血组心肌细胞凋亡率显著低于益气药组。结论:活血、益气中药均有抑制心衰左室重构和心肌细胞凋亡,延缓心衰发展的作用,且活血组8周的效果最佳。

不同治则中药单体对体外培养神经干细胞分化的影响

[目的]研究不同治则中药对体外培养大鼠神经干细胞分化及成熟的影响。[方法]体外培养胎鼠神经干细胞 ,按适当浓度接种于24孔板 ,以β-tublin代表非成熟神经元marker,以tau代表成熟神经元marker,RT -PCR方法检测β-tublin、tau的表达 ,观察黄芩苷、栀子苷 ,黄芪甲苷、三七总皂苷对神经干细胞分化及成熟的影响。[结果]结果表明 ,黄芩苷、栀子苷能诱导神经干细胞特异性分化成神经元 ,而联合应用黄芪甲苷、三七总皂苷能进一步促进分化神经元的成熟。[结论]不同治则中药 ,针对神经再生的增殖、分化过程 ,联合用药 。

益气活血法改善糖尿病肾病大鼠肾小管重吸收功能的研究

目的:观察益气活血药对黄芪丹参对糖尿病肾病模型大鼠尿蛋白、肾功能、肾小管重吸收的影响,并探讨其机制。方法:采用链脲佐菌素单次大剂量(55mg/kg)腹腔注射,诱导大鼠糖尿病肾病(DN),造模成功后随机分成模型组、益气活血组(黄芪和丹参注射液给药组)、格列喹酮组,同时设置正常对照组。于给药第6周尾静脉注射FITC-BSA,取各组大鼠的尿液检测24h尿蛋白量,血液测定血糖、血清尿素氮、血清肌酐、血清甘油三酯及血清总胆固醇;取肾脏制成组织匀浆测定LPO、SOD、GSH-Px和高级糖基化终产物(AGEs),肾脏石蜡切片用于PAS、HE及免疫组化染色。结果:与正常对照组比较,模型组大鼠体质量显著降低,血糖、24h尿蛋白量、血清肌酐、血清尿素氮、血清甘油三酯、血清总胆固醇均显著增加,肾组织中LPO和AGEs的含量显著增加(P<0.01),GSH-Px和SOD的活性显著下降(P<0.05)。益气活血药对可显著降低DN大鼠24h尿蛋白量(P<0.01),显著降低血清肌酐、血清尿素氮,血清甘油三酯和血清总胆固醇的水平(P<0.05),并可以降低DN大鼠肾组织中LPO和AGEs的含量(P<0.05),升高GSH-Px的活性(P<0.05)。肾脏切片染色结果显示益气活血药对可显著改善糖尿病肾脏肾小管的病变,并提高肾小管对FITC-BSA的重吸收能力。免疫组化结果表明黄芪丹参合用可以增加肾小管上皮细胞megalin表达。结论:益气活血法治疗可以改善糖尿病肾病大鼠肾小管的病变,减少蛋白尿,改善肾小管重吸收功能,其机制可能与增加肾脏组织的抗氧化活性,增加肾小管上皮细胞megalin表达有关。

益气养阴活血法对早期糖尿病肾病患者一氧化氮的影响

目的:观察以益气养阴活血法为主治疗早期糖尿病肾病(DN)患者的疗效及对一氧化氮(NO)的影响。方法:将入选的65例患者随机分为对照组(31例)和治疗组(34例),对照组予糖尿病教育、常规降血糖及对症处理,治疗组加用以益气养阴活血为主的中药治疗。结果:早期糖尿病肾病患者NO明显升高,治疗8周后,两组均能较好地降低症状积分、空腹血糖(FBG)及24h尿微量白蛋白排泄量(UAE)。治疗组在改善患者症状、降低UAE及N0水平方面,疗效明显优于对照组(P<0.05或P<0.01),对照组对NO改善不明显。结论:提示DN早期高N0生成与肾小球高滤过有关,益气养阴活血法为主可能通过降低肾脏NO水平,改善肾小球的高滤过。

益气活血化瘀祛痰法治疗冠心病心绞痛疗效的Meta分析

目的：运用Meta分析对应用益气活血化瘀祛痰法治疗冠心病心绞痛的疗效进行系统评价。方法：检索中国期刊全文数据库（CNKI）,维普中文期刊数据库（VIP）,中国生物医学文献光盘数据库（CBM disk）,万方数据库,纳入运用益气、活血、化瘀、祛痰中2种及以上为主要治疗方法,以中药为主要治疗药物,对照组采用西药治疗或中成药物治疗的随机对照试验。由2名研究者通过阅读文章题目和摘要,并结合全文,对文献进行资料提取,应用Revman 5.3软件对临床总有效率和心电图改变总有效率进行Meta分析。结果：共纳入研究文献12篇,Meta分析结果显示：在治疗冠心病心绞痛临床总有效率方面,以益气活血化瘀祛痰法为主要治疗方法与对照组比较具有统计学意义（OR=3.40,95%Cl=[2.43,4.76],Z=7.15,P〈0.000 01）;在心电图改善有效率方面,以益气活血化瘀祛痰法为主要治疗方法与对照组比较具有统计学意义（OR=2.61,95%Cl=[1.75,3.89],Z=4.69,P〈0.000 01）。结论：益气活血化瘀祛痰法治疗冠心病心绞痛临床疗效和心电图改善水平由于常规疗法,但本次纳入的文献质量一般,本次分析仍具有一定的局限性,仍需进一步进行合理的多中心随机双盲对照实验进行评价。

益气活血化痰法对老年高脂血症患者血管内皮功能及凝血系统的影响

目的:观察益气活血化痰法治疗老年高脂血症的临床疗效及对血管内皮功能和凝血系统的影响。方法:将96例老年高脂血症患者随机分为治疗组和对照组。治疗组56例,口服丹参决明颗粒,对照组40例,口服血脂康胶囊。观察指标包括血脂、血管内皮功能、凝血系统及安全性检测。结果:治疗后在降低血浆总胆固醇(TC)和低密度脂蛋白胆固醇(LDL-C)方面治疗组优于对照组(P<0.05)。血栓素 B_2(TXB_2)/6-酮-前列腺素 F_(1α)(6-keto-PGF_(1α))比值治疗后治疗组与对照组比较差异有显著性(P<0.05)。D-2聚体(D-D)、纤维蛋白原(FIB)两组治疗后比较差异有显著性(P<0.05)。结论:丹参决明颗粒具有调节老年高脂血症血脂代谢,改善血管内皮功能和凝血系统的作用。

益气活血中药对急性肺损伤机械通气后患者肺保护的临床研究

目的:观察益气活血中药对急性肺损伤(ALI)机械通气后的肺保护作用。方法:对符合纳入标准67例ALI患者按纳入的时间顺序随机分为2组,对照组32例给予常规综合治疗,观察组35例辅以益气活血中药治疗,60 m L/次,3次/天,连用14天。在服药后第3、7、14天比较患者血气分析、血流动力学、外周血中性粒细胞(PMN)计数指标及支气管镜显像等变化。结果:观察组在PMN、体循环血管阻力(SVR)、心率(HR)、血氧分压(Pa O2)、氧合指数(PO2/Fi O2)及p H值较对照组显著改善(P<0.05)。观察组气管镜下显像提示充血、水肿及炎症反应有显著好转。结论:益气活血中药辅助治疗能改善ALI患者的血流动力学、动脉血气及炎性指标,对肺有较好的保护作用,疗效确切。

人参川芎含药血清对氧糖剥夺/复氧培养神经干细胞ERK信号通路和增殖、活力的影响

目的观察人参、川芎组合对体外培养氧糖剥夺/复氧大鼠神经干细胞细胞外信号调节激酶(ex-tracellular-signal-responsive kinase,ERK)信号通路和增殖、活力的影响。方法神经干细胞培养分为5组:正常对照组、氧糖剥夺/复氧组、氧糖剥夺/复氧加人参药物血清组、氧糖剥夺/复氧加川芎药物血清组、氧糖剥夺/复氧加人参川芎药物血清组。免疫印迹方法观察ERK及p-ERK蛋白表达水平,5-溴脱氧尿核苷(5-bro-modeoxyuridine,BrdU)掺入法观察神经干细胞的增殖,噻唑蓝(methyl thiazolyl tetrazolium,MTT)检测细胞活性。结果 ERK磷酸化水平于复氧后10min、30min一过性升高,但4h、6h、1d各时间点均降至正常水平;取复氧后6h时间点观察发现,相比氧糖剥夺/复氧组,人参、川芎、人参川芎含药血清均可升高ERK磷酸化水平,促进复氧后1d时神经干细胞的增殖,提高其活力,且人参川芎联合运用优于单用人参和单用川芎。结论益气活血药物人参川芎可能通过ERK信号通路促进氧糖剥夺、复氧后大鼠神经干细胞的增殖,提高细胞活力,效果优于单用益气药物人参和活血药物川芎。

益气活血法治疗闭塞性动脉硬化症60例临床研究

目的:探讨益气活血中药方剂对早期闭塞性动脉硬化症(ASO)的治疗作用。方法:90例ASO患者随机分为2组,治疗2个月,观察2组治疗前后临床表现、血脂、血液流变学、血管内皮细胞功能、血流动力学等的变化。结果:冶疗组临床治愈率为61.67%,总有效率为100％;对照组临床治愈率为36.67％,总有效率为93.33%。对主要症状、体征的疗效,治疗组明显优于对照组(P<0.01)。对血脂的影响,治疗组治疗前后甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)差异有显著性(P<0.01);血清总胆固醇(TC)、极低密度脂蛋白胆固醇(VLDL-C)、载脂蛋白A_1(ApoA_1)、载脂蛋白B_(100)(ApoB_(100))亦有显著差异(P<0.05)。治疗组治疗后血液流变学各项指标较治疗前均明显下降(P<0.01);治疗后治疗组血浆血栓素B_2(TXB _2)、6-酮前列腺素F_(1α)(6-keto-PGF_(1α))恢复至正常(P<0.05),血浆内皮素(ET-1)水平降低,一氧化氮(NO)值增高(P<0.01)。两组的TXB_2及对照组TXB_2、6-keto-PGF_(1α),与正常组相比仍有显著差异(P<0.01)。治疗组治疗后股、腘动脉管腔宽度和血流量有增加,踝/肱指数明显升高(P<0.01)。结论:益气活血方剂黄芪通脉合剂治疗早期ASO能明显改善临床症状,并具有调节血脂代谢、抗血栓形成、保护血管内皮细胞功能等作用。