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The isolation and identification of apolipoprotein C-I in hormone-refractory prostate cancer using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry 被引量:3
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作者 Kaori Yamamoto-Ishikawa Hiroyoshi Suzuki +7 位作者 Masahiko Nezu Naoto Kamiya Takashi Imamoto Akira Komiya Kazuyuki Sogawa Takeshi Tomonaga Fumlo Nomur Tomohiko Ichikawa 《Asian Journal of Andrology》 SCIE CAS CSCD 2009年第3期299-307,共9页
Androgens play a central role in prostate cancer pathogenesis, and hence most of the patients respond to androgen deprivation therapies. However, patients tend to relapse with aggressive prostate cancer, which has bee... Androgens play a central role in prostate cancer pathogenesis, and hence most of the patients respond to androgen deprivation therapies. However, patients tend to relapse with aggressive prostate cancer, which has been termed as hormone refractory. To identify the proteins that mediate progression to the hormone-refractory state, we used protein-chip technology for mass profiling of patients' sera. This study included 16 patients with metastatic hormone-refractory prostate cancer who were initially treated with androgen deprivation therapy. Serum samples were collected from each patient at five time points: point A, pre-treatment; point B, at the nadir of the prostate- specific antigen (PSA) level; point C, PSA failure; point D, the early hormone-refractory phase; and point E, the late hormone-refractory phase. Using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry, we performed protein mass profiling of the patients' sera and identified a 6 640-Da peak that increased with disease progression. Target proteins were partially purified, and by amino acid sequencing the peak was identified as a fragment of apolipoprotein C-I (ApoC-I). Serum ApoC-I protein levels increased with disease progression. On immunohistochemical analysis, the ApoC-i protein was found localized to the cytoplasm of the hormone-refractory cancer cells. In this study, we showed an increase in serum ApoC-I protein levels in prostate cancer patients during their progression to the hormone-refractory state, which suggests that ApoC-I protein is related to progression of prostate cancer. However, as the exact role of ApoC-I in prostate cancer pathogenesis is unclear, further research is required. 展开更多
关键词 apolipoprotein C-I hormonal therapy PROGNOSIS prostate cancer surface-enhanced laser desorption/ionization time-offlight mass spectrometry
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Detection of nasopharyngeal carcinoma using surface-enhanced laser desorption and ionization mass spectrometry profiles of the serum proteome 被引量:2
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作者 Su-Mei Cao Jie-Kai YU +7 位作者 Qiu-Yan Chen Ning-Wei Li Yan-Qun Xiang Chao-Nan Qian Xun HU Chang-Qing Zhang Dan Xie Xiang Guo 《Chinese Journal of Cancer》 SCIE CAS CSCD 北大核心 2010年第8期721-728,共8页
Background and Objective: Early diagnosis of nasopharyngeal carcinoma (NPC) is difficult due to the insufficient specificity of the conventional examination method. This study was to investigate potential and consiste... Background and Objective: Early diagnosis of nasopharyngeal carcinoma (NPC) is difficult due to the insufficient specificity of the conventional examination method. This study was to investigate potential and consistent biomarkers for NPC, particularly for early detection of NPC. Methods: A proteomic pattern was identified in a training set (134 NPC patients and 73 control individuals) using the surface-enhanced laser desorption and ionization-mass spectrometry (SELDI-MS), and used to screen the test set (44 NPC patients and 25 control individuals) to determine the screening accuracy. To confirm the accuracy, it was used to test another group of 52 NPC patients and 32 healthy individuals at 6 months later. Results: Eight proteomic biomarkers with top-scored peak mass/charge ratios (m/z) of 8605 Da, 5320 Da, 5355 Da, 5380 Da, 5336 Da, 2791 Da, 7154 Da, and 9366 Da were selected as the potential biomarkers of NPC with a sensitivity of 90.9% (40/44) and a specificity of 92.0% (23/25). The performance was better than the current diagnostic method by using the Epstein-Barr virus (EBV) capsid antigen IgA antibodies (VCA/IgA). Similar sensitivity (88.5%) and specificity (90.6%) were achieved in another group of 84 samples. Conclusion: SELDI-MS profiling might be a potential tool to identify patients with NPC, particularly at early clinical stages. 展开更多
关键词 激光解吸电离 蛋白质组 鼻咽癌 表面增强 质谱法 生物标志物 SELDI 血清
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Two-dimensional gel electrophoresis and surface-enhanced laser desorption ionization-time of flight-mass spectrometry for detection of protein expression profiles in the hippocampus following closed brain injury 被引量:2
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作者 Qingming Shu Zhiqiang Li +3 位作者 Shuwang Yang Lingzhi Li Xiao Bai Yongliang Zhang 《Neural Regeneration Research》 SCIE CAS CSCD 2010年第23期1795-1801,共7页
Gene expression profile changes in brain regions following traumatic brain injury at the gene level cannot sufficiently elucidate gene expression time, expression amount, protein post-translational processing or modif... Gene expression profile changes in brain regions following traumatic brain injury at the gene level cannot sufficiently elucidate gene expression time, expression amount, protein post-translational processing or modification. Therefore, it is necessary to quantitatively analyze the gene expression profile using proteomic techniques. In the present study, we established a rat model of closed brain injury using Marmarou's weight-drop device, and investigated hippocampal differential protein expression using two-dimensional gel electrophoresis and surface-enhanced laser desorption ionization-time of flight-mass spectrometry. A total of 364 protein peaks were detected on weak cation exchange-2 protein chips, including 37 differential protein peaks. 345 protein peaks were detected on immobilized metal affinity capture arrays-Cu, including 12 differential protein peaks Further examination of these differential proteins revealed that glucose-regulated protein and proteasome subunit alpha type 3 expression were significantly upregulated post-injury. These results indicate that brain injury can alter protein expression in the hippocampus, and that glucose-regulated protein and proteasome subunit alpha type 3 are closely associated with the occurrence and development of traumatic brain injury. 展开更多
关键词 surface-enhanced laser desorption ionization-time of flight-mass spectrometry two-dimensional gel electrophoresis HIPPOCAMPUS PROTEOMICS brain injury neural regeneration
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MicrobMatcher: a microbial comparison software based on matrix-assisted laser desorption/ionization with time-of-flight mass spectrometry
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作者 Yizhen Fu Feng Liu Yalu Ye 《Journal of Biomedical Science and Engineering》 2011年第5期403-409,共7页
Matrix-assisted Laser Desorption/Ionization with Time-of-flight Mass Spectrometry (MALDI-TOFMS) was investigated as a method for the rapid identifica-tion of species. Current demand in microbial identi-fication is how... Matrix-assisted Laser Desorption/Ionization with Time-of-flight Mass Spectrometry (MALDI-TOFMS) was investigated as a method for the rapid identifica-tion of species. Current demand in microbial identi-fication is how to compare unknown strains to the known one quickly, semi-automatically and accurately. In this paper, we present a software tool that allows flexibly microbial matching in a user-friendly way, by letting the users to customize comparison parameters including: in vitro transcription enzyme, mass tolerance,minimum fragment length, intensity threshold and corresponding weights. We provide three spectral scoring functions to compute the affin-ity between the species. Therefore, the precision of microbial comparison increases. To test and verify this tool, we employed experimental spectral data based on MALDI-TOFMS and the gene sequences of E.coli and Salmonella. This software is written in Java for cross-platform intention. 展开更多
关键词 MICROBIAL Comparison Matrix-Assisted laser desorption/ionization with time-of-flight mass spectrometry
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Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry traces the geographical source of Biomphalaria pfeifferi and Bulinus forskali,involved in schistosomiasis transmission
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作者 Papa Mouhamadou Gaye EI Hadj Ibrahima Ndiaye +8 位作者 Souleymane Doucoure Doudou Sow Mapenda Gaye Ndiaw Goumballa Carole Cassagne Coralie L'Ollivier Oleg Medianikov Cheikh Sokhna Stephane Ranque 《Infectious Diseases of Poverty》 SCIE CAS CSCD 2024年第1期98-99,共2页
Background Freshwater snails of the genera Bulinus spp.,Biomphalaria spp.,and Oncomelania spp.are the main intermediate hosts of human and animal schistosomiasis.Identification of these snails has long been based on m... Background Freshwater snails of the genera Bulinus spp.,Biomphalaria spp.,and Oncomelania spp.are the main intermediate hosts of human and animal schistosomiasis.Identification of these snails has long been based on mor-phological and/or genomic criteria,which have their limitations.These limitations include a lack of precision for the morphological tool and cost and time for the DNA-based approach.Recently,Matrix-Assisted Laser Desorp-tion/lonization Time-Of-Flight(MALDI-TOF)mass spectrometry,a new tool used which is routinely in clinical microbi-ology,has emerged in the field of malacology for the identification of freshwater snails.This study aimed to evaluate the ability of MALDI-TOF MS to identify Biomphalaria pfeifferi and Bulinus forskali snail populations according to their geographicalorigin.Methods This study was conducted on 101 Bi.pfeifferi and 81 Bu.forskali snails collected in three distinct geo-graphical areas of Senegal(the North-East,South-East and central part of the country),and supplemented with wild and laboratory strains.Specimens which had previously been morphologically described were identified by MALDl-TOF MS[identification log score values(LSV)≥1.7],after an initial blind test using the pre-existing database.After DNA-based identification,new reference spectra of Bi.pfeiferi(n=10)and Bu.forskali(n=5)from the geographical areas were added to the MALDI-TOF spectral database.The final blind test against this updated database was per-formed to assess identification at the geographic source level.Results MALDI-TOF MS correctly identified 92.1%of 101 Bi.pfeifferi snails and 98.8%of 81 Bu.forskali snails.At the final blind test,88%of 166 specimens were correctly identified according to both their species and sampling site,with LSVs ranging from 1.74 to 2.70.The geographical source was adequately identified in 90.1%of 91 Bi.pfeifferi and 85.3%of 75 Bu.forskalii samples.Conclusions Our findings demonstrate that MALDI-TOF MS can identify and differentiate snail populations according to geographical origin.It outperforms the current DNA-based approaches in discriminating laboratory from wild strains.This inexpensive high-throughput approach is likely to further revolutionise epidemiological studies in areas which are endemic for schistosomiasis. 展开更多
关键词 Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry Snail Intermediate host Biomphalaria pfeifferi Bulinus forskali SCHISTOSOMIASIS Senegal
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Effect of surface-enhanced laser desorption/ionization time-of-flight mass spectrometry on identifing biomarkers of endometriosis 被引量:7
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作者 ZHANG Hong FENG Jie +3 位作者 CHANG Xiao-hong LI Zhong-xing WU Xiao-yi CUI Heng 《Chinese Medical Journal》 SCIE CAS CSCD 2009年第4期373-376,共4页
Background Endometriosis is a common gynecological disease. This study aimed to screen proteins that were expressed differently in patients with endometriosis versus normal controls using proteomic techniques, surface... Background Endometriosis is a common gynecological disease. This study aimed to screen proteins that were expressed differently in patients with endometriosis versus normal controls using proteomic techniques, surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS).Methods Protein chip SELDI-TOF-MS combines the advantages of microarray and mass spectrometry, and can screen latent markers in sera of patients with endometriosis. Serum samples from patients and normal volunteers were analyzed by SELDI-TOF-MS. Results After comparing the serum protein spectra of 36 patients with 24 normal controls, 24 differently expressed potential biomarkers (P 〈0.01) were identified. Using Biomarker Pattern software, we established a tree model of the 60 serum protein spectra. When using the three bJomarkers to classify the samples, the sensitivity for diagnosing endometriosis was 91.7%, specificity was 95.8%, and coincidence rate was 93.3%. Then we used serum samples from 12 patients and 8 normal controls to validate the tree model and report the sensitivity for diagnosing endometriosis was 91.7%, specificity was 75%, and coincidence rate was 85%. Conclusions SELDI-TOF-MS may be a useful tool in high-risk population screening for endometriosis. The identification and application of the biomarkers need to further study. 展开更多
关键词 ENDOMETRIOSIS PROTEOMICS surface-enhanced laser desorption/ionization mass spectrometry
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Studies on extracting solutions of endohedral rare-earth metallofullerenes by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
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作者 孙大勇 刘志强 +3 位作者 刘子阳 郭兴华 徐文国 刘淑莹 《Science China Chemistry》 SCIE EI CAS 1997年第6期590-599,共10页
Thirteen extracting solutions of rare-earth metallofullerenes containing La,Ce,Pr,Nd Sm,Eu,Gd,Tb,Dy,Ho,Er,Tm and Yb respectively have been investigated by means of matrix-assisted laser desorpuon/ ionization time-of-f... Thirteen extracting solutions of rare-earth metallofullerenes containing La,Ce,Pr,Nd Sm,Eu,Gd,Tb,Dy,Ho,Er,Tm and Yb respectively have been investigated by means of matrix-assisted laser desorpuon/ ionization time-of-flight mass spectrometry.The influences of the positive-ion/negative-ion mode,laser intensity,ma trix and mass discrimination to the analytical results are studied,based on which the optimal analytical conditions have been determined.The results show that the extracting solutions contain large quantities of rare-earth metallofullerenes besides empty fullerenes.On the basis of comparing their relative intensities,the different structure stabilities and solubilities of metallofullerenes with different rare-earth metals encapsulated into the fullerene cages,as well as some possible reasons to those differences,are discussed. 展开更多
关键词 METALLOFULLERENES FULLERENES laser desorption/ionization time-of-flight mass spectrometry
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Detection of Sialylated N-Linked Glycans by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry
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作者 PENG Yanfei XU Xiaojuan 《Wuhan University Journal of Natural Sciences》 CAS 2014年第3期245-252,共8页
Native and methyl-esterified sialylated glycans were analyzed with 2,4,6-trihydroxyacetophenone(THAP)and 2,5-dihydroxybenzoic acid(DHB)as matrix by a matrix-assisted laser desorption/ionization time-of-flight mass... Native and methyl-esterified sialylated glycans were analyzed with 2,4,6-trihydroxyacetophenone(THAP)and 2,5-dihydroxybenzoic acid(DHB)as matrix by a matrix-assisted laser desorption/ionization time-of-flight mass spectrometer(MALDI-TOF MS).High quality negative-ion spectra of commercial sialylated glycan were obtained with THAP as matrix.Detection limit of the glycan was less than 0.1 pmol.After methyl esterification of sialic acid(SA)residue,sialylated glycans were detected sensitively in the positive-ion mode using DHB as matrix.Neutral and sialylated glycans from the mixture of asialofetuin and fetuin were methylesterified and simultaneously recognized in one manipulation.Methyl esterification of SA residue offers a convenient and sensitive way to identify the structure of N-linked glycans for glycan profiling. 展开更多
关键词 sialic acid glycans methyl esterification matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF MS)
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血清蛋白质指纹图谱对紫杉类联合蒽环类化疗一线治疗乳腺癌的预测作用
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作者 葛小琴 赵菁 +1 位作者 叶晓贤 沈虹 《浙江医学》 CAS 2023年第4期391-394,F0003,共5页
目的寻找能预测紫杉类联合蒽环类(AT)一线治疗转移性乳腺癌疗效的潜在蛋白质标志物。方法以2015年8月至2018年7月浙江大学医学院附属第二医院经AT一线化疗的转移性三阴性乳腺癌的26例患者为对象,使用实体瘤疗效评价标准(RICIST)区分经过... 目的寻找能预测紫杉类联合蒽环类(AT)一线治疗转移性乳腺癌疗效的潜在蛋白质标志物。方法以2015年8月至2018年7月浙江大学医学院附属第二医院经AT一线化疗的转移性三阴性乳腺癌的26例患者为对象,使用实体瘤疗效评价标准(RICIST)区分经过2个疗程治疗后的化疗受益者和化疗无效者,使用血清蛋白质指纹图谱技术分析比较化疗受益者和化疗无效者的血清蛋白质谱的差异,并构建预测模型。结果经AT一线化疗,26例患者中17例有效,9例无效。表面增强激光解吸/电离飞行时间质谱(SELDI-TOF-MS)和人工神经网络(ANN)分析发现,两组患者间5个蛋白质峰存在差异。基于血清蛋白质指纹图谱构建的乳腺癌患者AT方案化疗疗效的潜在预测模型可有效区分化疗受益者与化疗无效者,灵敏度达1.000,特异度达0.889。结论血清蛋白质指纹图谱模型可有效筛选出可能从AT方案化疗中受益的转移性乳腺癌患者。 展开更多
关键词 乳腺癌 表面增强激光解吸/电离飞行时间质谱 人工神经网络 蛋白质组学
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血清中蛋白质组构型对结直肠癌的诊断意义 被引量:37
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作者 赵光 高春芳 +2 位作者 宋国英 李冬晖 王秀丽 《癌症》 SCIE CAS CSCD 北大核心 2004年第6期614-618,共5页
背景与目的:目前对结直肠癌诊断尚无敏感性和特异性均较高的血清学诊断指标,本研究旨在通过对血清中蛋白质组的质谱分析来鉴别结直肠癌、良性疾病和正常人血清蛋白质组构型的指纹图谱。方法:蛋白组学图谱通过表面增强激光解吸离子化飞... 背景与目的:目前对结直肠癌诊断尚无敏感性和特异性均较高的血清学诊断指标,本研究旨在通过对血清中蛋白质组的质谱分析来鉴别结直肠癌、良性疾病和正常人血清蛋白质组构型的指纹图谱。方法:蛋白组学图谱通过表面增强激光解吸离子化飞行时间质谱仪(surfaceenhancedlaserdesorption/ionization-timeofflight-massspectrometry,SELDI-TOF-MS)产生。对73例结直肠癌患者、31例正常人及16例结直肠良性疾病患者的血清进行分析,并反复分析寻找合理运算规则以确定能够区分癌症和非癌症的蛋白组学图谱。对独立的含120份双盲血清样本检测组分组,其中含73例结直肠癌患者的血清、31例正常人的血清及16例结直肠良性疾病患者的血清。结果:软件分析结果显示预测组中三类血清蛋白质在质荷比为4467Da、8131Da、8939Da、9192Da、9134Da、8221Da、5928Da、8324Da、11732Da9处含量有显著性差异,得到计算规则,其分类准确率为98.33%(118/120),敏感性为97.26%(71/73),特异性为100%(47/47);对检测组进行双盲检测,结果显示准确率为96.77%(116/120),敏感性和特异性分别为95.89%(70/73)、97.87%(46/47)。结论:利用SELDI-TOF-MS技术对结直肠癌患者、结直肠良性疾病患者及正常人血清的比较蛋白质组学分析,应用敏感性和特异性高的血清? 展开更多
关键词 飞行时间质谱 结直肠癌 血清 生物标记物 蛋白质组学
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胃癌患者血清比较蛋白质组学研究 被引量:20
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作者 高春芳 李冬晖 +3 位作者 赵光 郑国宝 王秀丽 许红兵 《解放军医学杂志》 CAS CSCD 北大核心 2005年第6期457-459,共3页
目的应用蛋白质质谱分析方法寻找胃癌鉴定的生物标志物.方法应用美国CipherGen公司金属亲和表面(IMAC3)芯片和蛋白芯片仪检测38例胃癌患者、82例正常人血清中的蛋白质相对含量.结果 38例胃癌患者与82例正常人在质荷比为1 723~14 048Da... 目的应用蛋白质质谱分析方法寻找胃癌鉴定的生物标志物.方法应用美国CipherGen公司金属亲和表面(IMAC3)芯片和蛋白芯片仪检测38例胃癌患者、82例正常人血清中的蛋白质相对含量.结果 38例胃癌患者与82例正常人在质荷比为1 723~14 048Da间有18种血清蛋白质含量有显著差异.在学习模式下38例胃癌患者及82例正常人均被正确分组,准确率为100%(120/120),灵敏度和特异性分别为100%(38/38)、100%(82/82);在检测模式下38例胃癌患者中有31例被正确论断,82例正常人中有81例被正确分组,灵敏度和特异性分别为81.6% (31/38)、98.8%(81/82).结论该方法可快速、准确检测胃癌,灵敏度、特异性高. 展开更多
关键词 胃肿瘤 肿瘤标记 生物学 表面增强激光解吸附离子化时间飞行质谱(SELDI-TOF-MS)
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利用血清中蛋白质组构型鉴定Dukes A期结直肠癌 被引量:11
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作者 高春芳 赵光 +2 位作者 郑国宝 李冬晖 王秀丽 《解放军医学杂志》 CAS CSCD 北大核心 2005年第6期460-462,共3页
目的 通过对血清蛋白质组的质谱分析,寻找用于Dukes A期结直肠癌鉴定的蛋白质组构型。方法 随机取10例Dukes A期及68例Dukes B、C、D期结直肠癌患者的血清作为预备组,另取10例Dukes A期及68例Dukes B、C、D期结直肠癌患者的血清作为... 目的 通过对血清蛋白质组的质谱分析,寻找用于Dukes A期结直肠癌鉴定的蛋白质组构型。方法 随机取10例Dukes A期及68例Dukes B、C、D期结直肠癌患者的血清作为预备组,另取10例Dukes A期及68例Dukes B、C、D期结直肠癌患者的血清作为检测组。预备组血清与金属亲和表面(IMAC3)芯片结合后,用蛋白芯片仪读取数据,分析可得到用于区分Dukes A期和Dukes B、C、D期结直肠癌患者的树状分类规则,并用双盲法测试检测组以证实其准确性。结果 预备组中两类血清蛋白质在质荷比为8 320、8 604、8 867、15 872Da等4处含量有显著差异,得到树状分类规则,其分类准确率为97 4%(76/78),灵敏度为100%(10/10),特异性为97 1%(66/68);对检测组进行双盲检测,结果显示准确率为94 9%(74/78),灵敏度和特异性分别为100%(10/10)、94 1%(64/68)。结论 该方法可快速、准确检测Dukes A期结直肠癌,灵敏度、特异性高。 展开更多
关键词 结直肠肿瘤 肿瘤标记 生物学 蛋白质组学 表面增强激光解吸附离子化时间飞行质谱(SELDI-TOF-MS)
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血清SELDI蛋白质指纹图谱在乳腺癌腋淋巴结转移中的应用研究 被引量:8
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作者 庞达 杨艳梅 +3 位作者 张国强 马玉彦 唐雅莉 龚建平 《中国肿瘤临床》 CAS CSCD 北大核心 2008年第17期1010-1014,共5页
目的:应用SELDI技术和生物信息学方法从血清中筛选乳腺癌蛋白质标志物并构建检测模型,为预测腋淋巴结(axillary lymph nodes,ALN)转移等提供可能的简便易行的方法。方法:应用SELDI-TOF-MS作为蛋白质组学摘要目的:应用SELDI技术和生物信... 目的:应用SELDI技术和生物信息学方法从血清中筛选乳腺癌蛋白质标志物并构建检测模型,为预测腋淋巴结(axillary lymph nodes,ALN)转移等提供可能的简便易行的方法。方法:应用SELDI-TOF-MS作为蛋白质组学摘要目的:应用SELDI技术和生物信息学方法从血清中筛选乳腺癌蛋白质标志物并构建检测模型,为预测腋研究平台,采用CM10芯片,对乳腺癌的血清进行了检测,探讨了乳腺癌患者血清蛋白质指纹图谱与是否发生ALN转移的关系,并结合生物信息学方法建立了相应的检测模型。结果:通过对ALN有(无)转移的乳腺癌患者血清蛋白指纹图谱数据的比较,找到了11个差异蛋白质峰(P<0.05),M/Z为M2164.16,M3269.90和M3272.31的3个蛋白质峰被选择用于构建分类决策树模型,该模型的交叉验证(测试组)总准确率为81.8%,ALN有转移的乳腺癌患者检出率为83.3%,ALN无转移的检出率为80%。结论:构建的分类决策树模型能达到区分ALN是否有转移的最佳效果,SELDI技术在确定乳腺癌患者是否发生腋淋巴结转移方面有一定的意义。 展开更多
关键词 乳腺癌 表面增强激光解吸电离-飞行时间质谱 生物信息学 蛋白质组学 CM10芯片
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结核性胸膜炎患者血清与胸腔积液蛋白质组的初步分析 被引量:15
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作者 彭德虎 胡锦兴 +3 位作者 石琳 林兆原 冯治宇 刘志辉 《实用医学杂志》 CAS 北大核心 2010年第23期4269-4271,共3页
目的:比较分析结核性胸膜炎患者血清与胸腔积液蛋白质组的异同,为探讨结核性胸膜炎的发生机制和寻找诊断标志物提供实验依据。方法:采用弱阳离子交换蛋白质芯片(WCX2)处理分析样本、应用表面增强激光解析电离飞行时间质谱(SELDI-TOF-MS... 目的:比较分析结核性胸膜炎患者血清与胸腔积液蛋白质组的异同,为探讨结核性胸膜炎的发生机制和寻找诊断标志物提供实验依据。方法:采用弱阳离子交换蛋白质芯片(WCX2)处理分析样本、应用表面增强激光解析电离飞行时间质谱(SELDI-TOF-MS)技术,以蛋白质/多肽质荷比(m/z)区分不同的蛋白质/多肽,对5例结核性胸膜炎患者血清与胸腔积液蛋白质组进行描述性分析。结果:(1)在5例患者血清中检测到134种血清蛋白质/多肽,其中76种在胸腔积液中未出现;在5例患者胸腔积液中检测到269种蛋白质/多肽,其中211种在血液中未出现。(2)在5例患者血液中均检测到m/z分别为2660、3191、4208、5903、7764、9287等6种蛋白质/多肽;在5例患者胸腔积液中均检测到m/z分别为2416、2660、2900、2940等4种蛋白质/多肽;在5例患者血液和胸腔积液中均检测到m/z为2660的蛋白质/多肽。结论:结核性胸膜炎患者胸腔积液蛋白质组远比其血清蛋白质组复杂,对其进行深入研究有助于明晰结核性胸膜炎的发生机制和寻找诊断标志物。 展开更多
关键词 结核 胸膜 蛋白质组 表面增强激光解析电离飞行时间质谱
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急性心肌梗死病程不同时期患者血清蛋白质组学分析 被引量:9
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作者 高春芳 郑国宝 +7 位作者 赵光 魏晓真 孟雪 王秀丽 李冬晖 许红兵 王全晖 宋国英 《解放军医学杂志》 CAS CSCD 北大核心 2005年第6期465-466,共2页
目的 探讨蛋白质质谱分析对急性心肌梗死病程不同时期的鉴别意义。方法 应用美国CipherGen公司金属亲和表面(IMAC3)芯片和蛋白芯片仪检测153例心肌梗死患者血清标本(其中刚入院45例,3h标本12例,6h标本22例,9h标本24例,12h标本24例,24... 目的 探讨蛋白质质谱分析对急性心肌梗死病程不同时期的鉴别意义。方法 应用美国CipherGen公司金属亲和表面(IMAC3)芯片和蛋白芯片仪检测153例心肌梗死患者血清标本(其中刚入院45例,3h标本12例,6h标本22例,9h标本24例,12h标本24例,24h标本16例,48h标本10例)中的蛋白质相对含量。结果 不同时期急性心肌梗死患者在质荷比为4kD^12kD间有10种血清蛋白质含量有显著差异。不同时期的患者均被正确判断,准确率为100%(153/153),灵敏度和特异性分别为100%(45/45)和100%(108/108)。结论 该方法可快速、准确检测各期急性心肌梗死,灵敏度、特异性高。 展开更多
关键词 心肌梗死 蛋白质组学 表面增强激光解吸附离子化时间飞行质谱(SELDI-TOF-MS)
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基于表面增强激光解析离子化飞行时间质谱技术的慢性肾衰竭中医湿证尿液中相关蛋白研究 被引量:8
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作者 郝一鸣 洪名超 +2 位作者 王文静 金亚明 王忆勤 《中国中西医结合杂志》 CAS CSCD 北大核心 2012年第11期1496-1499,共4页
目的采用表面增强激光解析离子化飞行时间质谱(surface-enhanced laser desorption/ionization time of flight mass spectrometry,SELDI-TOF-MS)技术研究慢性肾衰竭(chronic renal failure,CRF)中医湿证患者的尿液蛋白标志物。方法采集... 目的采用表面增强激光解析离子化飞行时间质谱(surface-enhanced laser desorption/ionization time of flight mass spectrometry,SELDI-TOF-MS)技术研究慢性肾衰竭(chronic renal failure,CRF)中医湿证患者的尿液蛋白标志物。方法采集CRF湿证患者90例和非湿证患者60例的尿液,采用H4蛋白芯片技术进行尿液蛋白质组学研究,用蛋白芯片阅读器PBSⅡ对芯片进行扫描、分析。结果 (1)湿证组与非湿证组尿液样本的蛋白质图谱在质荷比1000-20000范围内检测到25个差异蛋白峰(P<0.01)。(2)经生物信息学分析建立CRF中医湿证尿液蛋白预测模型,得到M/Z8654.96、M/Z2081.65、M/Z18667.3和M/Z2242.14共4个差异蛋白峰组成的生物标记物可以将湿证组和非湿证组样本较好地分类,其正确率84.7%,灵敏度为92.2%,特异性为73.3%。(3)湿证组与非湿证组尿液中差异蛋白峰经SwissProt数据库鉴定,可能为7种蛋白质。结论初步筛选出CRF中医湿证的尿液蛋白标志物,建立了CRF中医湿证尿液蛋白预测模型,通过数据库对尿液蛋白标志物进行了鉴定,为CRF中医湿证的临床辨证提供了一定的实验依据。 展开更多
关键词 慢性肾衰竭 中医湿证 尿液 表面增强激光解析离子化飞行时间质谱
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血清蛋白质指纹图谱与人工神经网络模型在肺癌诊断中的应用 被引量:7
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作者 韩明勇 刘奇 +1 位作者 余捷凯 郑树 《山东大学学报(医学版)》 CAS 北大核心 2008年第6期604-607,共4页
目的筛选肺癌相关标志物并建立诊断肺癌的蛋白质谱模型。方法应用表面增强激光解吸电离飞行时间质谱(SELDI-TOF-MS)技术检测了86例肺癌、80例健康对照样本的血清蛋白质质谱,结合人工神经网络建立肺癌诊断模型。结果从肺癌组与健康对... 目的筛选肺癌相关标志物并建立诊断肺癌的蛋白质谱模型。方法应用表面增强激光解吸电离飞行时间质谱(SELDI-TOF-MS)技术检测了86例肺癌、80例健康对照样本的血清蛋白质质谱,结合人工神经网络建立肺癌诊断模型。结果从肺癌组与健康对照组中筛选出了4个蛋白质荷比峰建立肺癌诊断模型,该诊断模型的特异性为100%(95%的置信区间为93.9%~100.0%),敏感性为93.6%(87.6%-96.4%),准确率为96.7%(88.1%。98.3%)。结论成功建立了肺癌诊断模型,该模型在肺癌的诊断中具有较高的敏感性和特异性。 展开更多
关键词 肺肿瘤 表面增强激光解吸电离飞行时间质谱 生物信息学 诊断 蛋白组学
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应用SELDI-TOF-MS技术初步建立结直肠癌区域淋巴转移分类树模型 被引量:5
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作者 高春芳 范乃军 +2 位作者 王秀丽 李冬晖 赵光 《解放军医学杂志》 CAS CSCD 北大核心 2010年第2期121-125,共5页
目的寻找血清中与结直肠癌淋巴结转移相关的特异性生物标志物。方法应用表面增强激光解吸离子化飞行时间质谱仪(SELDI-TOF-MS)检测结直肠癌患者血清的蛋白质谱,利用配套软件进行蛋白质峰值鉴定、聚类并建立分类树模型。70例伴区域淋巴... 目的寻找血清中与结直肠癌淋巴结转移相关的特异性生物标志物。方法应用表面增强激光解吸离子化飞行时间质谱仪(SELDI-TOF-MS)检测结直肠癌患者血清的蛋白质谱,利用配套软件进行蛋白质峰值鉴定、聚类并建立分类树模型。70例伴区域淋巴结转移的结直肠癌患者和75例年龄、性别匹配,无区域淋巴结转移的结直肠癌患者作为训练组,通过软件分析得到分类树模型,以35例伴区域淋巴结转移的结直肠癌患者和30例年龄、性别匹配,无区域淋巴结转移的结直肠癌患者作为测试组进行独立样本的双盲验证。结果共识别出46种组间差异蛋白,其中由质荷比(M/Z)为3104、3781、5867、7970、9290五种蛋白构成的分类树模型可以有效鉴别结直肠癌患者伴或不伴区域淋巴结转移,灵敏度和特异度分别为94.3%(66/70)和100.0%(75/75),经双盲验证其灵敏度、特异度、阳性预测值分别为91.4%(32/35)、96.7%(29/30)、97.0%(32/33)。结论所建立的分类树模型可以准确鉴别结直肠癌患者伴或不伴区域淋巴结转移,对结直肠癌的术前筛查有重要价值。 展开更多
关键词 光谱法 质量 表面增强激光解吸离子化飞行时间 结直肠肿瘤 生物学标记 蛋白质组学 淋巴转移
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激光捕获显微切割联合SELDI蛋白质芯片筛选肺腺癌早期诊断标志蛋白 被引量:5
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作者 田应选 杨拴盈 +3 位作者 南岩东 张潍 余捷凯 郑树 《四川大学学报(医学版)》 CAS CSCD 北大核心 2009年第1期157-161,共5页
目的探讨应用激光显微切割(LCM)联合表面增强激光解吸离子化飞行时间质谱蛋白质芯片(SEL-DI-TOF-MS)及模式识别分类技术—支持向量机(SVM)筛选肺腺癌标志蛋白的可行性。方法将6例新鲜肺腺癌组织标本及其配对的4例正常肺组织制备8μm厚... 目的探讨应用激光显微切割(LCM)联合表面增强激光解吸离子化飞行时间质谱蛋白质芯片(SEL-DI-TOF-MS)及模式识别分类技术—支持向量机(SVM)筛选肺腺癌标志蛋白的可行性。方法将6例新鲜肺腺癌组织标本及其配对的4例正常肺组织制备8μm厚度冰冻切片;改良HE染色;用LCM技术选择性获取同质腺癌细胞和配对正常肺组织细胞。应用PBSⅡ+型SELDI-TOF-MS分析仪(IMAC芯片)分析腺癌及其配对正常细胞的蛋白质表达谱,比对差异点;应用SVM筛选并验证候选标志蛋白的判别效能。结果平均每个LCM帽子的激光点数约4000shots,获得了同质性>95%的肿瘤细胞和正常细胞。比较腺癌和配对正常细胞之间的SELDI谱图,共筛选出84个蛋白峰。将差异最明显的10个蛋白峰作为候选标志蛋白。和正常组织相比,6种蛋白在腺癌中呈低表达,4种蛋白在腺癌中呈高表达,差异有统计学意义(P<0.05)。初步筛选出3191m/z蛋白峰作为腺癌诊断标志蛋白。结论LCM联合SELDI蛋白质芯片技术有可能筛选出敏感性高、特异性强的肺腺癌标志蛋白;该技术将为肺腺癌早期诊断研究提供新的强有力的工具。 展开更多
关键词 激光捕获显微切割 表面增强激光解吸离子化飞行时间质谱 蛋白质组学 肺腺癌 早期诊断
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非霍奇金淋巴瘤患者化疗前后血清蛋白质谱动态变化及其标志蛋白的筛选 被引量:5
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作者 凌家瑜 孙晓非 +5 位作者 张星 甄子俊 夏奕 罗文标 林慧 郑磊 《癌症》 SCIE CAS CSCD 北大核心 2008年第10期1065-1069,共5页
背景与目的:采用现代治疗方法治疗非霍奇金淋巴瘤(non-Hodgkin’s lymphoma,NHL)完全缓解率可达70%~80%,但仍有40%~50%患者最终会复发,而微小残留病灶是复发的根源。本研究应用SELDI蛋白芯片技术,分析初诊NHL患者与正常人群血清蛋白... 背景与目的:采用现代治疗方法治疗非霍奇金淋巴瘤(non-Hodgkin’s lymphoma,NHL)完全缓解率可达70%~80%,但仍有40%~50%患者最终会复发,而微小残留病灶是复发的根源。本研究应用SELDI蛋白芯片技术,分析初诊NHL患者与正常人群血清蛋白质谱的差异和化疗前后血清蛋白质谱的变化,寻找NHL血清小分子标记物。方法:采用表面增强激光解吸电离飞行时间质谱分析技术(surface-enhanced laser desorption/ionization time of flight mass spectrometry,SELDI-TOF-MS)分析3组血清标本:44例NHL初诊组、51例正常对照组和44例完全缓解组(NHL患者自身配对)。应用Ciphergen ProteinChip 3.1软件进行原始数据的校正和分析。结果:与正常对照组比较,有1个差异蛋白峰(M11710)在NHL初诊组高表达而在CR组降低至接近正常(P<0.05);另外有9个差异蛋白峰(M3322、M4355、M6445、M6646、M8581、M8708、M8918、M13959、M15149)在NHL初诊组血清中低表达,而在CR组升高至接近正常(P<0.05)。通过建立决策树模型,发现初诊NHL患者血清存在有5个候选标志蛋白,高表达为M11710,低表达为M8581、M15149、M6646和M8918。结论:应用SELDI-TOF-MS分析可在化疗前后筛选出NHL患者血清中的标志蛋白,有可能在微小残留病监测、早期复发预测、疗效判断等方面提供有用的信息。 展开更多
关键词 非霍奇金淋巴瘤 表面增强激光解吸电离飞行时间质谱 蛋白标记
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