Biosensors are widely used in immunoassay. The biosensor chip carries a receptor which is used in immunoassay and the chip properties have an important influence on the detecting sensitivity of the biosensor. This pap...Biosensors are widely used in immunoassay. The biosensor chip carries a receptor which is used in immunoassay and the chip properties have an important influence on the detecting sensitivity of the biosensor. This paper describes a polystyrene\|based biosensor chip developed and used as part of a surface plasmon resonance (SPR) biosensor. The SPR biosensor has a much higher detecting sensitivity than enzyme\|linked immunoserbent assay (ELISA).展开更多
Modern miniaturization and the digitalization of characterization instruments greatly facilitate the diffusion of technological advances in new fields and generate innovative applications.The concept of a portable,ine...Modern miniaturization and the digitalization of characterization instruments greatly facilitate the diffusion of technological advances in new fields and generate innovative applications.The concept of a portable,inexpensive and semi-automated biosensing platform,or lab-on-a-chip,is a vision shared by many researchers and venture industries.Under this scope,we present a semiconductor monolithic integration approach to conduct surface plasmon resonance studies.This technology is already commonly used for biochemical characterization in pharmaceutical industries,but we have reduced the technological platform to a few nanometers in scale on a semiconductor chip.We evaluate the signal quality of this nanophotonic device using hyperspectral-imaging technology,and we compare its performance with that of a standard prism-based commercial system.Two standard biochemical agents are employed for this characterization study:bovine serum albumin and inactivated influenza A virus.Time resolutions of data acquisition varying between 360 and 2.2 s are presented,yielding 2.731025–1.531026 RIU resolutions,respectively.展开更多
文摘Biosensors are widely used in immunoassay. The biosensor chip carries a receptor which is used in immunoassay and the chip properties have an important influence on the detecting sensitivity of the biosensor. This paper describes a polystyrene\|based biosensor chip developed and used as part of a surface plasmon resonance (SPR) biosensor. The SPR biosensor has a much higher detecting sensitivity than enzyme\|linked immunoserbent assay (ELISA).
基金The authors acknowledge the financial contribution from the Natural Science and Engineering Research Council of Canada(NSERC Strategic grant STPGP 350501-07)the Canada Research Chair in Quantum Semiconductors Program and the Vanier Scholarship CGS program.
文摘Modern miniaturization and the digitalization of characterization instruments greatly facilitate the diffusion of technological advances in new fields and generate innovative applications.The concept of a portable,inexpensive and semi-automated biosensing platform,or lab-on-a-chip,is a vision shared by many researchers and venture industries.Under this scope,we present a semiconductor monolithic integration approach to conduct surface plasmon resonance studies.This technology is already commonly used for biochemical characterization in pharmaceutical industries,but we have reduced the technological platform to a few nanometers in scale on a semiconductor chip.We evaluate the signal quality of this nanophotonic device using hyperspectral-imaging technology,and we compare its performance with that of a standard prism-based commercial system.Two standard biochemical agents are employed for this characterization study:bovine serum albumin and inactivated influenza A virus.Time resolutions of data acquisition varying between 360 and 2.2 s are presented,yielding 2.731025–1.531026 RIU resolutions,respectively.
