AFLP fingerprinting of the 98 main sweetpotato varieties planted in China has been constructed. Using 17 AFLP primer combinations which were selected from 1 208 primer combinations and generated the most amounts of po...AFLP fingerprinting of the 98 main sweetpotato varieties planted in China has been constructed. Using 17 AFLP primer combinations which were selected from 1 208 primer combinations and generated the most amounts of polymorphic bands, AFLP analysis of the 98 main sweetpotato varieties gave a total of 410 clear polymorphic bands with an average of 24.12 polymorphic bands per primer combination. Each one of the 98 sweetpotato varieties could be clearly distinguished by EcoR I-cta/Mse I-ggc primer combination which generated the most polymorphic bands. AFLP-based genetic distance ranged from 0.0546 to 0.5709 with an average of 0.3799. The dendrogram based on AFLP markers indicated that sweetpotato varieties coming from the same regions or having same parents were clustered in the same groups. Analysis of molecular variance （AMOVA） revealed greater variations within regions （94.08%） than among regions （5.92%）. Thus, the genetic variations mainly existed within regions, while the variations among regions were very low in the tested sweetpotato varieties. Significant genetic variations existed between ＂Northern＂ and ＂Southern＂ sweetpotato varieties when Yangtze River was used as the dividing line.展开更多
Sweetpotato,Ipomoea batatas(L.) Lam.,is a globally important food crop and usually grown on arid-and semi-arid lands.Therefore,investigating the molecular mechanism of drought tolerance will provide important informat...Sweetpotato,Ipomoea batatas(L.) Lam.,is a globally important food crop and usually grown on arid-and semi-arid lands.Therefore,investigating the molecular mechanism of drought tolerance will provide important information for the improvement of drought tolerance in this crop.In this study,transcriptome analysis of the drought-tolerant sweetpotato line Xushu 55-2 was conducted on Illumina HiSeq 2500 platform.A total of 86.69 Gb clean data were generated and assembled into 2 671 693 contigs,222 073 transcripts,and 73 636 unigenes.In total,11 359 differentially expressed genes(DEGs) were identified after PEG6000 treatment,in which 7 666 were up-regulated and 3 693 were down-regulated.Of the 11 359 DEGs,10 192 DEGs were annotated in at least one database,and the remaining 1 167 DEGs were unknown.Abscisic acid(ABA),ethylene(ETH),and jasmonic acid(JA) signalling pathways play a major role in drought tolerance of sweetpotato.Drought-inducible transcription factors were identified,some of which have been reported to be associated with drought tolerance and others are unknown in plants.In addition,7 643 SSRs were detected.This study not only reveals insights into the molecular mechanism of drought tolerance in sweetpotato but also provides the candidate genes involved in drought tolerance of this crop.展开更多
Geranylgeranyl pyrophosphate synthase(GGPS) plays an important role in the biosynthesis of carotenoids. In a previous study, the IbGGPS gene was isolated from a sweetpotato, Ipomoea batatas(L.) Lam., line Nongdafu 14 ...Geranylgeranyl pyrophosphate synthase(GGPS) plays an important role in the biosynthesis of carotenoids. In a previous study, the IbGGPS gene was isolated from a sweetpotato, Ipomoea batatas(L.) Lam., line Nongdafu 14 with high carotenoid contents, but its role and underlying mechanisms in carotenoid biosynthesis in sweetpotato were not investigated. In the present study, the IbGGPS gene was introduced into a sweetpotato cv. Lizixiang and the contents of β-carotene, β-cryptoxanthin, zeaxanthin and lutein were significantly increased in the storage roots of the IbGGPSoverexpressing sweetpotato plants. Further analysis showed that IbGGPS gene overexpression systematically upregulated the genes involved in the glycolytic, 2-C-methyl-D-erythritol-4-phosphate(MEP) and carotenoid pathways,which increased the carotenoid contents in the transgenic plants. These results indicate that the IbGGPS gene has the potential for use in improving the carotenoid contents in sweetpotato and other plants.展开更多
基金China Agriculture Research System (Sweetpotato) and Chinese Universities Scientific Fund (2012YJ008)
文摘AFLP fingerprinting of the 98 main sweetpotato varieties planted in China has been constructed. Using 17 AFLP primer combinations which were selected from 1 208 primer combinations and generated the most amounts of polymorphic bands, AFLP analysis of the 98 main sweetpotato varieties gave a total of 410 clear polymorphic bands with an average of 24.12 polymorphic bands per primer combination. Each one of the 98 sweetpotato varieties could be clearly distinguished by EcoR I-cta/Mse I-ggc primer combination which generated the most polymorphic bands. AFLP-based genetic distance ranged from 0.0546 to 0.5709 with an average of 0.3799. The dendrogram based on AFLP markers indicated that sweetpotato varieties coming from the same regions or having same parents were clustered in the same groups. Analysis of molecular variance （AMOVA） revealed greater variations within regions （94.08%） than among regions （5.92%）. Thus, the genetic variations mainly existed within regions, while the variations among regions were very low in the tested sweetpotato varieties. Significant genetic variations existed between ＂Northern＂ and ＂Southern＂ sweetpotato varieties when Yangtze River was used as the dividing line.
基金supported by the earmarked fund for China Agriculture Research System (CARS-10,Sweetpotato)
文摘Sweetpotato,Ipomoea batatas(L.) Lam.,is a globally important food crop and usually grown on arid-and semi-arid lands.Therefore,investigating the molecular mechanism of drought tolerance will provide important information for the improvement of drought tolerance in this crop.In this study,transcriptome analysis of the drought-tolerant sweetpotato line Xushu 55-2 was conducted on Illumina HiSeq 2500 platform.A total of 86.69 Gb clean data were generated and assembled into 2 671 693 contigs,222 073 transcripts,and 73 636 unigenes.In total,11 359 differentially expressed genes(DEGs) were identified after PEG6000 treatment,in which 7 666 were up-regulated and 3 693 were down-regulated.Of the 11 359 DEGs,10 192 DEGs were annotated in at least one database,and the remaining 1 167 DEGs were unknown.Abscisic acid(ABA),ethylene(ETH),and jasmonic acid(JA) signalling pathways play a major role in drought tolerance of sweetpotato.Drought-inducible transcription factors were identified,some of which have been reported to be associated with drought tolerance and others are unknown in plants.In addition,7 643 SSRs were detected.This study not only reveals insights into the molecular mechanism of drought tolerance in sweetpotato but also provides the candidate genes involved in drought tolerance of this crop.
基金supported by the National Key Research and Development Program of China(2019YFD1001302 and 2019YFD1001300)the China Agriculture Research System of MOF and MARA(CARS-10-Sweetpotato)。
文摘Geranylgeranyl pyrophosphate synthase(GGPS) plays an important role in the biosynthesis of carotenoids. In a previous study, the IbGGPS gene was isolated from a sweetpotato, Ipomoea batatas(L.) Lam., line Nongdafu 14 with high carotenoid contents, but its role and underlying mechanisms in carotenoid biosynthesis in sweetpotato were not investigated. In the present study, the IbGGPS gene was introduced into a sweetpotato cv. Lizixiang and the contents of β-carotene, β-cryptoxanthin, zeaxanthin and lutein were significantly increased in the storage roots of the IbGGPSoverexpressing sweetpotato plants. Further analysis showed that IbGGPS gene overexpression systematically upregulated the genes involved in the glycolytic, 2-C-methyl-D-erythritol-4-phosphate(MEP) and carotenoid pathways,which increased the carotenoid contents in the transgenic plants. These results indicate that the IbGGPS gene has the potential for use in improving the carotenoid contents in sweetpotato and other plants.
