Swertisin contents in rat urine,feces and tissues were determined by reversed-phase high-performance liquid chromatography(RP-HPLC) method.Chromatographic separations were performed on a C18 column with acetonitrile...Swertisin contents in rat urine,feces and tissues were determined by reversed-phase high-performance liquid chromatography(RP-HPLC) method.Chromatographic separations were performed on a C18 column with acetonitrile-water(23:77,v/v) as the mobile phase.The calibration curves were linear over the ranges of 0.175-35.0μg/mL for rat urine,0.5-60.0μg/mL for rat feces,and 0.014 to 53.0μg/mL for all tissues.The inter-and intra-day precisions and accuracy for all measured samples were satisfactory.The fully validated method was applied for tissue distribution and excretion of swertisin in rat urine and bile after intravenous administration.The maximum level of swertisin was found in kidney,which reached 83.87± 6.36μg/g.In rat heart,swertisin was hardly detected under used experimental conditions.Swetisin level in liver,kidney,stomach,smooth muscle and skeletal muscle continued to decrease from 5 to 60min.Swertisin showed increasing tendency in intestine,spleen and testis tissues at scheduled time points.Detectable swertisin was found in brain and lung tissue.Totally 11.9% swertisin dose was cumulatively excreted from urine in 60h after intravenous administration.There was small amount of swertisin in rat feces and the cumulative excretion level reached 4.59% of intravenous dose in 60h.展开更多
运用HP20型大孔吸附树脂、聚酰胺柱色谱、Sephadex-LH20型葡聚糖凝胶柱色谱和YMC制备型高效液相色谱柱(ODS)等色谱方法,从海南产射干叶中分离了4种黄酮碳苷.采用UV,1 H NMR,13 C NMR,MS等波谱学方法,结合熔点测定、盐酸-镁粉反应、三氯...运用HP20型大孔吸附树脂、聚酰胺柱色谱、Sephadex-LH20型葡聚糖凝胶柱色谱和YMC制备型高效液相色谱柱(ODS)等色谱方法,从海南产射干叶中分离了4种黄酮碳苷.采用UV,1 H NMR,13 C NMR,MS等波谱学方法,结合熔点测定、盐酸-镁粉反应、三氯化铝显色反应等物理和化学方法,鉴定了它们的结构.4种黄酮碳苷分别为化合物Ⅰ:2″-O-鼠李糖基异牡荆素;化合物Ⅱ:2″-O-鼠李糖基当药素;化合物Ⅲ:异牡荆素;化合物Ⅳ:当药素.4种化合物均为首次从鸢尾科植物中分离.展开更多
基金Supported by the Excellent Young Scholars Research Foundation of Beijing Institute of Technology(2007Y0612)
文摘Swertisin contents in rat urine,feces and tissues were determined by reversed-phase high-performance liquid chromatography(RP-HPLC) method.Chromatographic separations were performed on a C18 column with acetonitrile-water(23:77,v/v) as the mobile phase.The calibration curves were linear over the ranges of 0.175-35.0μg/mL for rat urine,0.5-60.0μg/mL for rat feces,and 0.014 to 53.0μg/mL for all tissues.The inter-and intra-day precisions and accuracy for all measured samples were satisfactory.The fully validated method was applied for tissue distribution and excretion of swertisin in rat urine and bile after intravenous administration.The maximum level of swertisin was found in kidney,which reached 83.87± 6.36μg/g.In rat heart,swertisin was hardly detected under used experimental conditions.Swetisin level in liver,kidney,stomach,smooth muscle and skeletal muscle continued to decrease from 5 to 60min.Swertisin showed increasing tendency in intestine,spleen and testis tissues at scheduled time points.Detectable swertisin was found in brain and lung tissue.Totally 11.9% swertisin dose was cumulatively excreted from urine in 60h after intravenous administration.There was small amount of swertisin in rat feces and the cumulative excretion level reached 4.59% of intravenous dose in 60h.
文摘运用HP20型大孔吸附树脂、聚酰胺柱色谱、Sephadex-LH20型葡聚糖凝胶柱色谱和YMC制备型高效液相色谱柱(ODS)等色谱方法,从海南产射干叶中分离了4种黄酮碳苷.采用UV,1 H NMR,13 C NMR,MS等波谱学方法,结合熔点测定、盐酸-镁粉反应、三氯化铝显色反应等物理和化学方法,鉴定了它们的结构.4种黄酮碳苷分别为化合物Ⅰ:2″-O-鼠李糖基异牡荆素;化合物Ⅱ:2″-O-鼠李糖基当药素;化合物Ⅲ:异牡荆素;化合物Ⅳ:当药素.4种化合物均为首次从鸢尾科植物中分离.