The present study was to screen and identify a biocontrol strain with antagonistic effect against Sporisorium scitaminea Sydow. 16 S rDNA gene fragments were amplified by universal primers P0 and P6. The results showe...The present study was to screen and identify a biocontrol strain with antagonistic effect against Sporisorium scitaminea Sydow. 16 S rDNA gene fragments were amplified by universal primers P0 and P6. The results showed that 16 S rDNA gene fragment with the size of 1 533 bp was obtained,and its sequence had the homology of 99% with the sequence of Bacillus subtilis. The morphological characteristics of the strain were as follows： the mycelium of strain HAS was short-rod shape,and could move; Gram staining was positive; 3% KOH solubility test was negative; flagellum was grown around the cells; spores were grown in the middle,elliptic,and cysts were slightly swelled. The strain was preliminarily identified as B. subtilis. Dual culture with pathogenic fungi showed that the strain had good inhibitory effect against various pathogenic fungi causing diseases on sugarcane and other crops. Therefore,strain HAS isolated in the study was identified to be B.subtilis,which had good control effect against S. scitaminea.展开更多
[ Objective] The paper aimed to screen sugarcane varieties and species layout suitable for Hainan sugarcane area. [ Method] Using mixed spores of Ustilago scitaminea Sydow, smut resistance of 21 major sugarcane variet...[ Objective] The paper aimed to screen sugarcane varieties and species layout suitable for Hainan sugarcane area. [ Method] Using mixed spores of Ustilago scitaminea Sydow, smut resistance of 21 major sugarcane varieties in national sugarcane regional trial as well as ROC22 and ROC16 were identified through artificial inoculation test and field natural incidence. [ Result] Yunzhe 99-596, Yuegan 42, Guitang 29 and Funong 39 reached highly resistant and resistant levels to smut in artificial inoculation test. [ Conclusion] Most of the varieties showed better resistance to smut in newly planted sugarcane and ratoon sugarcane except Yunzhe 03-103, Liucheng 03-1137 and ROC22.展开更多
基金Supported by Natural Science Foundation of Hainan Province(314120)Fundamental Research Fund for Central Public-interest Scientific Institutions(ITBB130502)Special Fund of China Agricultural Industry Research Sys-tem(nycytx-24)
文摘The present study was to screen and identify a biocontrol strain with antagonistic effect against Sporisorium scitaminea Sydow. 16 S rDNA gene fragments were amplified by universal primers P0 and P6. The results showed that 16 S rDNA gene fragment with the size of 1 533 bp was obtained,and its sequence had the homology of 99% with the sequence of Bacillus subtilis. The morphological characteristics of the strain were as follows： the mycelium of strain HAS was short-rod shape,and could move; Gram staining was positive; 3% KOH solubility test was negative; flagellum was grown around the cells; spores were grown in the middle,elliptic,and cysts were slightly swelled. The strain was preliminarily identified as B. subtilis. Dual culture with pathogenic fungi showed that the strain had good inhibitory effect against various pathogenic fungi causing diseases on sugarcane and other crops. Therefore,strain HAS isolated in the study was identified to be B.subtilis,which had good control effect against S. scitaminea.
基金Supported by National Natural Science Foundation of China(31471555)Natural Science Foundation of Hainan Province(314120)Special Fund of China Agricultural Industry Research System(CARS-20-2-25)
文摘[ Objective] The paper aimed to screen sugarcane varieties and species layout suitable for Hainan sugarcane area. [ Method] Using mixed spores of Ustilago scitaminea Sydow, smut resistance of 21 major sugarcane varieties in national sugarcane regional trial as well as ROC22 and ROC16 were identified through artificial inoculation test and field natural incidence. [ Result] Yunzhe 99-596, Yuegan 42, Guitang 29 and Funong 39 reached highly resistant and resistant levels to smut in artificial inoculation test. [ Conclusion] Most of the varieties showed better resistance to smut in newly planted sugarcane and ratoon sugarcane except Yunzhe 03-103, Liucheng 03-1137 and ROC22.
