Objective:To explore the structural and functional characteristics of microbiota in oropharynx of subhealthy children with gastrointestinal heat retention syndrome(GHRS)differentiated by traditional Chinese medicine,a...Objective:To explore the structural and functional characteristics of microbiota in oropharynx of subhealthy children with gastrointestinal heat retention syndrome(GHRS)differentiated by traditional Chinese medicine,and screen the biometric operational taxonomic units(OTUs)to assist the clinical diagnosis.Methods:We recruited children according to the“GHRS diagnostic scale”,collected their oropharyngeal swabs,and sequenced the 16 SrDNA V4 region.We described the bacterial structure with alpha-indexes,beta-distances,and relative abundances;moreover,we screened the differential genera/OTUs with Wilcoxon rank-sum test,Metagenome Seq analysis,and linear discriminant analysis effect size(LEf Se)analysis,in which biometric OTUs were selected to construct the receiver operating characteristic curve to verify the diagnostic value.The bacterial function was predicted with Kyoto Encyclopedia of Genes and Genomes pathways according to 16S rDNA gene by using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States.Results:The study population was composed of 10 children with GHRS and 10 healthy control children.GHRS children were more likely to overeat(gluttony,P=.033).Alpha-indexes,such as Sobs,abundancebased coverage estimator,Bootstrap,and Qstat,were significantly higher in the GHRS group,while betadistances did not exhibit any significant intergroup differences.There were 9 differently distributed nonpredominant genera between the groups in Wilcoxon rank-sum test,as well as 13 non-predominant genera in Metagenome Seq analysis and 3 non-predominant OTUs in LEfSe analysis.OTU44 and OTU196 were used to construct the receiver operating characteristic curve,and the area under curve was 0.92.Predicted functions showed that pathways related to oxidative phosphorylation and carbon metabolism were enriched in healthy control samples,while the pathway related to renin secretion was remarkably enriched in GHRS samples.Conclusion:Unique oropharyngeal microbial structure and function were identified in GHRS children.OTU44 and OTU196 were specific OTUs,which could be used as biomarkers of GHRS to assist clinical diagnosis.展开更多
Objective： To investigate a method for quantitative differential diagnosis of damp-heat and cold-damp impeding syndrome of rheumatoid arthritis （RA） in Chinese medicine （CM）. Methods： Laboratory parameters were ...Objective： To investigate a method for quantitative differential diagnosis of damp-heat and cold-damp impeding syndrome of rheumatoid arthritis （RA） in Chinese medicine （CM）. Methods： Laboratory parameters were collected from 306 patients with RA. The clinical symptoms and laboratory parameters were compared between patients with these two syndromes （158 with RA of damp-heat impeding syndrome, and 148 with RA of cold-damp impeding syndrome）, and a regression equation was established to facilitate discrimination of the two RA syndromes. Results： There were significant differences in disease activity score in 28 joints [DAS28 （4）], erythrocyte sedimentation rate （ESR）, white blood cell count （WBC）, C-reactive protein （CRP）, platelet count （PLT）, albumin （ALB） and globulin （GLB） between the two syndrome of RA （P〈0.05）. Logistic regression analysis showed that the parameters ESR, WBC, CRP, joint pyrexia, joint cold, thirst, sweating, aversion to wind and cold, and cold extremities were statistically useful to discriminate damp-heat from cold-damp impeding syndrome. The regression equation was as follows： P=1/{1＋exp[-（3.0-0.021X1-0.196X2-0.163X3- 1.559X4＋1.504X5-0.927X6-1.039X7＋1.070X8＋1.330X9）]}. The independent variables X1-X9 were ESR, WBC, CRP, hot joint, cold joint, thirst, sweating, aversion to wind and cold, and cold limbs. A P value 〉 0.5 signified cold-damp impeding syndrome, and a P value 〈 0.5 signified damp-heat impeding syndrome. The accuracy was 90.2%. Conclusion： The regression equation may be useful for discriminating damp-heat from cold-damp impeding syndrome of RA.展开更多
Recent studies have revealed that the property of drug is mainly associated with the body's substance and energy metabolism. The present study aimed to evaluate the drug property of Poria, called Fuling(FL) in tra...Recent studies have revealed that the property of drug is mainly associated with the body's substance and energy metabolism. The present study aimed to evaluate the drug property of Poria, called Fuling(FL) in traditional Chinese medicine(TCM), in terms of its effects on the substance and energy metabolism in rat models of cold-deficiency and heat-deficiency syndromes, compared with Aconiti Lateralis Radix Praeparaia, called Fuzi(FZ) in TCM, with hot property, and Anemarrhenae Rhizoma, called Zhimu(ZM) in TCM, with cold property, as reference drugs, respectively. The appearance score, toe and rectal temperatures of the animals treated were assessed at different time points. Several indices in vivo correlated with substance and energy metabolism(glucokinas, phosphoglycerate kinase, cytochrome c reductase, cytochrome c oxydase, and Na^+-K^+-ATPase), endocrine system(triiodothyronine, thyroxine, and 17-hydroxycorticosteroid), nervous system(acetylcholin esterase), and cyclic nucleotide system were determined. The changes in appearance score and indices in vivo suggested the successful establishment of cold-deficiency and heat-deficiency syndrome models. FZ reversed the decreased levels of indices(substance and energy metabolism and endocrine system) and alleviated the syndrome of cold-deficiency model, and ZM showed obviously therapeutic effect on heat-deficiency syndrome(appearance score, substance and energy metabolism, and endocrine system). FL could alleviate cold-deficiency syndrome and raise the decreased levels of glucokinas, phosphoglycerate kinase, cytochrome c reductase and triiodothyronine in cold-deficiency model, but had no significant effect on heat-deficiency syndrome. Drug property of FL was inferred as trending to "flat and warm", which still need further study. It was advisable to adopt both cold-deficiency and heat-deficiency models to study the drugs with "flat" property.展开更多
目的:观察柴胡桂枝干姜汤治疗上热下寒型失眠的临床疗效。方法:选择2023年4—10月安阳市中医院脑病三科门诊诊治的100例上热下寒型失眠患者,按照随机数字表法分为对照组和试验组,每组各50例。对照组给予艾司唑仑片治疗,试验组给予柴胡...目的:观察柴胡桂枝干姜汤治疗上热下寒型失眠的临床疗效。方法:选择2023年4—10月安阳市中医院脑病三科门诊诊治的100例上热下寒型失眠患者,按照随机数字表法分为对照组和试验组,每组各50例。对照组给予艾司唑仑片治疗,试验组给予柴胡桂枝干姜汤加减治疗。比较两组患者的临床疗效、不良反应积分及治疗前后中医证候积分、匹兹堡睡眠治疗指数、世界卫生组织生存质量测定量表简表(the world health organization quality of life scale,WHOQOL-BREF)积分变化情况。结果:两组患者治疗后中医证候积分低于本组治疗前,且治疗后试验组低于对照组(P<0.05)。试验组有效率为92.0%,高于对照组的86.0%,差异有统计学意义(P<0.05)。两组患者治疗后匹兹堡睡眠指数低于本组治疗前,且治疗后试验组低于对照组(P<0.05)。两组患者治疗后WHOQOL-BREF评分高于本组治疗前,且治疗后试验组高于对照组(P<0.05)。对照组不良反应积分为(2.72±0.73)分,试验组不良反应积分为(0.60±0.67)分,差异具有统计学意义(P<0.05)。结论:柴胡桂枝干姜汤加减治疗上热下寒型失眠临床疗效确切,可改善患者的临床症状,提高睡眠质量及生活质量。展开更多
lasma cortisol levels and leukocyte glucocorticosteroid receptor (GCR) contents were assayed in 10 patients with Deficiency-Cold Syndrome, 10 patients with Deficiency-Heat Syndrome, and 10healthy subjects as normal co...lasma cortisol levels and leukocyte glucocorticosteroid receptor (GCR) contents were assayed in 10 patients with Deficiency-Cold Syndrome, 10 patients with Deficiency-Heat Syndrome, and 10healthy subjects as normal control. The results showed practically normal plasma cortisol levels in the De-ticiency-Cold Group, but the leukocyte GCR contents were markedly lowered. In the Deficiency-Heatgroup, plasma cortisol levels were remarkably elevated and the leukocyte GCR contents also tended to behigher than normal. The quantity of plasma cortisol concentrations multiplied by leukocyte GCR contentscan be taken as a sensitive index for the differentiation of Cold and Heat natures in Deficiency Syndromes.展开更多
基金supported by the National Natural Science Foundation of China(81373769)the Scientific Research and Postgraduate Training Co-construction Project of Beijing Municipal Education Commission(1000062520115)the Province Natural Science Foundation of Beijing(7172131)。
文摘Objective:To explore the structural and functional characteristics of microbiota in oropharynx of subhealthy children with gastrointestinal heat retention syndrome(GHRS)differentiated by traditional Chinese medicine,and screen the biometric operational taxonomic units(OTUs)to assist the clinical diagnosis.Methods:We recruited children according to the“GHRS diagnostic scale”,collected their oropharyngeal swabs,and sequenced the 16 SrDNA V4 region.We described the bacterial structure with alpha-indexes,beta-distances,and relative abundances;moreover,we screened the differential genera/OTUs with Wilcoxon rank-sum test,Metagenome Seq analysis,and linear discriminant analysis effect size(LEf Se)analysis,in which biometric OTUs were selected to construct the receiver operating characteristic curve to verify the diagnostic value.The bacterial function was predicted with Kyoto Encyclopedia of Genes and Genomes pathways according to 16S rDNA gene by using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States.Results:The study population was composed of 10 children with GHRS and 10 healthy control children.GHRS children were more likely to overeat(gluttony,P=.033).Alpha-indexes,such as Sobs,abundancebased coverage estimator,Bootstrap,and Qstat,were significantly higher in the GHRS group,while betadistances did not exhibit any significant intergroup differences.There were 9 differently distributed nonpredominant genera between the groups in Wilcoxon rank-sum test,as well as 13 non-predominant genera in Metagenome Seq analysis and 3 non-predominant OTUs in LEfSe analysis.OTU44 and OTU196 were used to construct the receiver operating characteristic curve,and the area under curve was 0.92.Predicted functions showed that pathways related to oxidative phosphorylation and carbon metabolism were enriched in healthy control samples,while the pathway related to renin secretion was remarkably enriched in GHRS samples.Conclusion:Unique oropharyngeal microbial structure and function were identified in GHRS children.OTU44 and OTU196 were specific OTUs,which could be used as biomarkers of GHRS to assist clinical diagnosis.
基金supported by the Scientific Program of Traditional Chinese Medicine of Chongqing Municipal Health Bureau,China (2008-1-15)the National Natural Science Foundation of China(No.30973827)
文摘Objective： To investigate a method for quantitative differential diagnosis of damp-heat and cold-damp impeding syndrome of rheumatoid arthritis （RA） in Chinese medicine （CM）. Methods： Laboratory parameters were collected from 306 patients with RA. The clinical symptoms and laboratory parameters were compared between patients with these two syndromes （158 with RA of damp-heat impeding syndrome, and 148 with RA of cold-damp impeding syndrome）, and a regression equation was established to facilitate discrimination of the two RA syndromes. Results： There were significant differences in disease activity score in 28 joints [DAS28 （4）], erythrocyte sedimentation rate （ESR）, white blood cell count （WBC）, C-reactive protein （CRP）, platelet count （PLT）, albumin （ALB） and globulin （GLB） between the two syndrome of RA （P〈0.05）. Logistic regression analysis showed that the parameters ESR, WBC, CRP, joint pyrexia, joint cold, thirst, sweating, aversion to wind and cold, and cold extremities were statistically useful to discriminate damp-heat from cold-damp impeding syndrome. The regression equation was as follows： P=1/{1＋exp[-（3.0-0.021X1-0.196X2-0.163X3- 1.559X4＋1.504X5-0.927X6-1.039X7＋1.070X8＋1.330X9）]}. The independent variables X1-X9 were ESR, WBC, CRP, hot joint, cold joint, thirst, sweating, aversion to wind and cold, and cold limbs. A P value 〉 0.5 signified cold-damp impeding syndrome, and a P value 〈 0.5 signified damp-heat impeding syndrome. The accuracy was 90.2%. Conclusion： The regression equation may be useful for discriminating damp-heat from cold-damp impeding syndrome of RA.
基金supported by National Basic Research Program of China(No.2013CB531803)
文摘Recent studies have revealed that the property of drug is mainly associated with the body's substance and energy metabolism. The present study aimed to evaluate the drug property of Poria, called Fuling(FL) in traditional Chinese medicine(TCM), in terms of its effects on the substance and energy metabolism in rat models of cold-deficiency and heat-deficiency syndromes, compared with Aconiti Lateralis Radix Praeparaia, called Fuzi(FZ) in TCM, with hot property, and Anemarrhenae Rhizoma, called Zhimu(ZM) in TCM, with cold property, as reference drugs, respectively. The appearance score, toe and rectal temperatures of the animals treated were assessed at different time points. Several indices in vivo correlated with substance and energy metabolism(glucokinas, phosphoglycerate kinase, cytochrome c reductase, cytochrome c oxydase, and Na^+-K^+-ATPase), endocrine system(triiodothyronine, thyroxine, and 17-hydroxycorticosteroid), nervous system(acetylcholin esterase), and cyclic nucleotide system were determined. The changes in appearance score and indices in vivo suggested the successful establishment of cold-deficiency and heat-deficiency syndrome models. FZ reversed the decreased levels of indices(substance and energy metabolism and endocrine system) and alleviated the syndrome of cold-deficiency model, and ZM showed obviously therapeutic effect on heat-deficiency syndrome(appearance score, substance and energy metabolism, and endocrine system). FL could alleviate cold-deficiency syndrome and raise the decreased levels of glucokinas, phosphoglycerate kinase, cytochrome c reductase and triiodothyronine in cold-deficiency model, but had no significant effect on heat-deficiency syndrome. Drug property of FL was inferred as trending to "flat and warm", which still need further study. It was advisable to adopt both cold-deficiency and heat-deficiency models to study the drugs with "flat" property.
文摘目的:观察柴胡桂枝干姜汤治疗上热下寒型失眠的临床疗效。方法:选择2023年4—10月安阳市中医院脑病三科门诊诊治的100例上热下寒型失眠患者,按照随机数字表法分为对照组和试验组,每组各50例。对照组给予艾司唑仑片治疗,试验组给予柴胡桂枝干姜汤加减治疗。比较两组患者的临床疗效、不良反应积分及治疗前后中医证候积分、匹兹堡睡眠治疗指数、世界卫生组织生存质量测定量表简表(the world health organization quality of life scale,WHOQOL-BREF)积分变化情况。结果:两组患者治疗后中医证候积分低于本组治疗前,且治疗后试验组低于对照组(P<0.05)。试验组有效率为92.0%,高于对照组的86.0%,差异有统计学意义(P<0.05)。两组患者治疗后匹兹堡睡眠指数低于本组治疗前,且治疗后试验组低于对照组(P<0.05)。两组患者治疗后WHOQOL-BREF评分高于本组治疗前,且治疗后试验组高于对照组(P<0.05)。对照组不良反应积分为(2.72±0.73)分,试验组不良反应积分为(0.60±0.67)分,差异具有统计学意义(P<0.05)。结论:柴胡桂枝干姜汤加减治疗上热下寒型失眠临床疗效确切,可改善患者的临床症状,提高睡眠质量及生活质量。
文摘lasma cortisol levels and leukocyte glucocorticosteroid receptor (GCR) contents were assayed in 10 patients with Deficiency-Cold Syndrome, 10 patients with Deficiency-Heat Syndrome, and 10healthy subjects as normal control. The results showed practically normal plasma cortisol levels in the De-ticiency-Cold Group, but the leukocyte GCR contents were markedly lowered. In the Deficiency-Heatgroup, plasma cortisol levels were remarkably elevated and the leukocyte GCR contents also tended to behigher than normal. The quantity of plasma cortisol concentrations multiplied by leukocyte GCR contentscan be taken as a sensitive index for the differentiation of Cold and Heat natures in Deficiency Syndromes.
