Severe pneumonia is one of the most serious infectious diseases.Delayed intervention may lead to pulmonary fibrosis,which greatly threatens people’s life and health.Blood stasis syndrome is an important underlying sy...Severe pneumonia is one of the most serious infectious diseases.Delayed intervention may lead to pulmonary fibrosis,which greatly threatens people’s life and health.Blood stasis syndrome is an important underlying syndrome throughout the evolution of severe pneumonia-pulmonary fibrosis.Xuebijing injection(XBJ)was developed under the theoretical system of“Three syndromes and three methods”,demonstrating a good efficacy in treating severe pneumonia and pulmonary fibrosis due to its effect of removing blood stasis and dispersing toxins.Previous studies have shown that XBJ can protect vascular endothelial function,improve coagulation function and regulate immunity by inhibiting inflammatory.Hence,the research hypothesis is put forward that XBJ treats blood stasis syndrome by removing blood stasis and dredging blood vessels,to inhibit the disease progress of severe pneumonia to pulmonary fibrosis.Further researches are need to confirm the function and explore the mechanism of XBJ.展开更多
Objective To investigate the correlation of platelet and coagulation function with blood stasis syndrome(BSS)in coronary heart disease(CHD).Methods The protocol for this meta-analysis was registered on PROSPERO(CRD420...Objective To investigate the correlation of platelet and coagulation function with blood stasis syndrome(BSS)in coronary heart disease(CHD).Methods The protocol for this meta-analysis was registered on PROSPERO(CRD42019129452).PubMed,Excerpta Medica Database(Embase),the Cochrane Library,and China National Knowledge Infrastructure(CNKI)were searched from inception to 1st June,2020.Trials were considered eligible if they enrolled BSS and non-BSS(NBSS)patients with CHD and provided information on platelet and coagulation function.The platelet function,coagulation function,and fibrinolytic activity were compared between the BSS and NBSS groups.Forest plots were generated to show the SMDs or ESs with corresponding 95%CIs for each study.Subgroup analysis and sensitivity analysis were performed to explore potential sources of heterogeneity.Results The systematic search identified 1,583 articles.Thirty trials involving 10,323 patients were included in the meta-analysis.The results showed that mean platelet volume,platelet distribution width,platelet aggregation rate,platelet P selectin,fibrinogen,plasminogen activator inhibitor-1(PAI-1),thromboxane B2(TXB2),6-keto-prostaglandin F1alpha(6-keto-PGF1α),and TXB2/6-keto-PGF1αwere higher in the BSS group than in the NBSS group(P<0.05 or P<0.01).Activated partial thromboplastin time was lower in the BSS group than in the NBSS group in the acute phase of CHD(P<0.01).The R and K values in thromboelastography and tissue plasminogen activator(t-PA)and t-PA/PAI-1 were lower in the BSS group than in the NBSS group(all P<0.01).No difference was found in the results of platelet count,plateletcrit,maximum amplitude,von Willebrand factor,prothrombin time,thrombin time,international normalized ratio,etc.between groups.Conclusions Increased platelet function,hypercoagulability,and decreased fibrinolytic activity were found among CHD patients with BSS.展开更多
Objective:To establish a novel cardiocentesis method for withdrawing venous blood from the right atrium,and to improve an acute blood stasis rat model using an ice bath and epinephrine hydrochloride(Epi)while consider...Objective:To establish a novel cardiocentesis method for withdrawing venous blood from the right atrium,and to improve an acute blood stasis rat model using an ice bath and epinephrine hydrochloride(Epi)while considering the 3Rs(reduction,refinement,and replacement)of humane animal experimentation.Methods:An acute blood stasis model was established in male Sprague-Dawley rats by subcutaneous injection(s.c.)Epi(1.2mg/kg)administration at 0h,followed by a 5-min exposure to an ice-bath at 2h and s.c.Epi administration at 4h.Control rats received physiological saline.Rats were fasted overnight and treated with Angelicae Sinensis Lateralis Radix(ASLR)and Pheretima the following day.Venous blood was collected using our novel cardiocentesis method and used to test whole blood viscosity(WBV),prothrombin time(PT),activated partial thromboplastin time(APTT),and fibrinogen(FIB)8ntent.Results:The rats survived the novel cardiocentesis technique;WBV value returned to normal while hematological parameters such as hemoglobin level and red blood cell count were restored to>94%of the corresponding values in normal rats following a 14-day recovery.Epi(1.2 mg/kg,s.c.)combined with a 5-min exposure to the ice bath replicated the acute blood stasis rat model and was associated with the highest WBV value.In rats showing acute blood stasis,ASLR treatment[4g/(kg-d)for 8 days]decreased WBV by 9.98%,11.09%,9.34%,9.00%,7.66%,and 7.03%(P<0.05),while Pheretima treatment[2.6g/(kg?d),for 8 days]decreased WBV by 25.49%,25.94%,16.28%,17.76%,11.07%,and 7.89%(P<0.01)at shear rates of 1,3,10,30,100,and 180 s'1,respectively.Furthermore,Pheretima treatment increased APTT significantly(P<0.01).Conclusions:We presented a stable,reproducible,and improved acute blood stasis rat model,which could be applied to screen drugs for promoting blood circulation and eliminating blood stasis.展开更多
Objective： To investigate the effects of different directions of moxibustion therapy on hemorheology in rat models with blood stasis due to cold retention. Methods： A total of 32 Wistar rats were randomly divided in...Objective： To investigate the effects of different directions of moxibustion therapy on hemorheology in rat models with blood stasis due to cold retention. Methods： A total of 32 Wistar rats were randomly divided into 4 groups, based on the random digits table, including a normal group, a model group, a moxibustion along the meridian group, and a moxibustion against the meridian group, with 8 rats in each group. Except the normal group, the other 3 groups were used to make the rat models with blood stasis due to cold retention. Rats in the moxibustion along and against the meridian groups accepted moxibustion therapy in different directions on the trunk segment of the Bladder Meridian after successful modeling. Moxibustion for 10 min every day, and 7 d as a course of treatment. Two courses of treatment（a total of 14 d） were carried out. Quantitative score of signs and symptoms change was observed once a week, with a total of 7 times. Hemorheological indexes of rats were detected when the treatment finished. Results： The quantitative scores of signs and symptoms in the model, moxibustion along the meridian and against the meridian groups were significantly higher than those in the normal group after modeling（P〈0.05）. When the treatment finished, the quantitative score of signs and symptoms in the model group was significantly higher than that in the normal group（P〈0.05）; the quantitative scores of signs and symptoms of rats in the moxibustion along and against the meridian groups were significantly lower than that in the model group（P〈0.05）; the quantitative score of signs and symptoms in the moxibustion along the meridian group was significantly lower than that in the moxibustion against the meridian group（P〈0.05）; the whole blood viscosity and erythrocyte aggregation index in the model group were significantly higher than those in the normal group（P〈0.05）, however the increases of erythrocyte hematocrit and erythrocyte sedimentation rate were not obvious（P〈0.05）; whole blood viscosity and erythrocyte aggregation index in the moxibustion along and against the meridian groups were significantly lower than those in the model group（P〈0.05）, while there were no statistically significant differences between the moxibustion along the meridian group and moxibustion against the meridian group（P〈0.05）. Conclusion： Moxibustion along and against the meridian both showed different degrees of improvement for hemorrheology and symptoms of blood stasis due to cold retention. Improvement for symptoms of blood stasis due to cold retention was better in the moxibustion along the meridian group than that in the moxibustion against the meridian group. The influence on rat hemorrheology showed no significant difference between moxibustion along the meridian and moxibustion against the meridian.展开更多
基金the second batch of“Ten thousand plan”-National high level talents special support plan(W02020052).
文摘Severe pneumonia is one of the most serious infectious diseases.Delayed intervention may lead to pulmonary fibrosis,which greatly threatens people’s life and health.Blood stasis syndrome is an important underlying syndrome throughout the evolution of severe pneumonia-pulmonary fibrosis.Xuebijing injection(XBJ)was developed under the theoretical system of“Three syndromes and three methods”,demonstrating a good efficacy in treating severe pneumonia and pulmonary fibrosis due to its effect of removing blood stasis and dispersing toxins.Previous studies have shown that XBJ can protect vascular endothelial function,improve coagulation function and regulate immunity by inhibiting inflammatory.Hence,the research hypothesis is put forward that XBJ treats blood stasis syndrome by removing blood stasis and dredging blood vessels,to inhibit the disease progress of severe pneumonia to pulmonary fibrosis.Further researches are need to confirm the function and explore the mechanism of XBJ.
基金Supported by the Authorized Project of China Academy of Chinese Medical Sciences(No.ZZ13-036-4)the Special Fund of Xiyuan Hospital of China Academy of Chinese Medical Sciences for the Cultivation of the National Natural Science Foundation of China(No.XY20-01)。
文摘Objective To investigate the correlation of platelet and coagulation function with blood stasis syndrome(BSS)in coronary heart disease(CHD).Methods The protocol for this meta-analysis was registered on PROSPERO(CRD42019129452).PubMed,Excerpta Medica Database(Embase),the Cochrane Library,and China National Knowledge Infrastructure(CNKI)were searched from inception to 1st June,2020.Trials were considered eligible if they enrolled BSS and non-BSS(NBSS)patients with CHD and provided information on platelet and coagulation function.The platelet function,coagulation function,and fibrinolytic activity were compared between the BSS and NBSS groups.Forest plots were generated to show the SMDs or ESs with corresponding 95%CIs for each study.Subgroup analysis and sensitivity analysis were performed to explore potential sources of heterogeneity.Results The systematic search identified 1,583 articles.Thirty trials involving 10,323 patients were included in the meta-analysis.The results showed that mean platelet volume,platelet distribution width,platelet aggregation rate,platelet P selectin,fibrinogen,plasminogen activator inhibitor-1(PAI-1),thromboxane B2(TXB2),6-keto-prostaglandin F1alpha(6-keto-PGF1α),and TXB2/6-keto-PGF1αwere higher in the BSS group than in the NBSS group(P<0.05 or P<0.01).Activated partial thromboplastin time was lower in the BSS group than in the NBSS group in the acute phase of CHD(P<0.01).The R and K values in thromboelastography and tissue plasminogen activator(t-PA)and t-PA/PAI-1 were lower in the BSS group than in the NBSS group(all P<0.01).No difference was found in the results of platelet count,plateletcrit,maximum amplitude,von Willebrand factor,prothrombin time,thrombin time,international normalized ratio,etc.between groups.Conclusions Increased platelet function,hypercoagulability,and decreased fibrinolytic activity were found among CHD patients with BSS.
基金Supported by the National Scie nee and Tech no logy Major Project for Major New Drugs Innovation and Development of China(No.2009ZX09502-023-4)National Technology Support Program(No.2006BAI09B06)。
文摘Objective:To establish a novel cardiocentesis method for withdrawing venous blood from the right atrium,and to improve an acute blood stasis rat model using an ice bath and epinephrine hydrochloride(Epi)while considering the 3Rs(reduction,refinement,and replacement)of humane animal experimentation.Methods:An acute blood stasis model was established in male Sprague-Dawley rats by subcutaneous injection(s.c.)Epi(1.2mg/kg)administration at 0h,followed by a 5-min exposure to an ice-bath at 2h and s.c.Epi administration at 4h.Control rats received physiological saline.Rats were fasted overnight and treated with Angelicae Sinensis Lateralis Radix(ASLR)and Pheretima the following day.Venous blood was collected using our novel cardiocentesis method and used to test whole blood viscosity(WBV),prothrombin time(PT),activated partial thromboplastin time(APTT),and fibrinogen(FIB)8ntent.Results:The rats survived the novel cardiocentesis technique;WBV value returned to normal while hematological parameters such as hemoglobin level and red blood cell count were restored to>94%of the corresponding values in normal rats following a 14-day recovery.Epi(1.2 mg/kg,s.c.)combined with a 5-min exposure to the ice bath replicated the acute blood stasis rat model and was associated with the highest WBV value.In rats showing acute blood stasis,ASLR treatment[4g/(kg-d)for 8 days]decreased WBV by 9.98%,11.09%,9.34%,9.00%,7.66%,and 7.03%(P<0.05),while Pheretima treatment[2.6g/(kg?d),for 8 days]decreased WBV by 25.49%,25.94%,16.28%,17.76%,11.07%,and 7.89%(P<0.01)at shear rates of 1,3,10,30,100,and 180 s'1,respectively.Furthermore,Pheretima treatment increased APTT significantly(P<0.01).Conclusions:We presented a stable,reproducible,and improved acute blood stasis rat model,which could be applied to screen drugs for promoting blood circulation and eliminating blood stasis.
基金supported by National Basic Research Program of China(973 Program)Special Project for Cultivation of Postgraduates' Innovative Abilities in Shanghai University of Traditional Chinese Medicine~~
文摘Objective： To investigate the effects of different directions of moxibustion therapy on hemorheology in rat models with blood stasis due to cold retention. Methods： A total of 32 Wistar rats were randomly divided into 4 groups, based on the random digits table, including a normal group, a model group, a moxibustion along the meridian group, and a moxibustion against the meridian group, with 8 rats in each group. Except the normal group, the other 3 groups were used to make the rat models with blood stasis due to cold retention. Rats in the moxibustion along and against the meridian groups accepted moxibustion therapy in different directions on the trunk segment of the Bladder Meridian after successful modeling. Moxibustion for 10 min every day, and 7 d as a course of treatment. Two courses of treatment（a total of 14 d） were carried out. Quantitative score of signs and symptoms change was observed once a week, with a total of 7 times. Hemorheological indexes of rats were detected when the treatment finished. Results： The quantitative scores of signs and symptoms in the model, moxibustion along the meridian and against the meridian groups were significantly higher than those in the normal group after modeling（P〈0.05）. When the treatment finished, the quantitative score of signs and symptoms in the model group was significantly higher than that in the normal group（P〈0.05）; the quantitative scores of signs and symptoms of rats in the moxibustion along and against the meridian groups were significantly lower than that in the model group（P〈0.05）; the quantitative score of signs and symptoms in the moxibustion along the meridian group was significantly lower than that in the moxibustion against the meridian group（P〈0.05）; the whole blood viscosity and erythrocyte aggregation index in the model group were significantly higher than those in the normal group（P〈0.05）, however the increases of erythrocyte hematocrit and erythrocyte sedimentation rate were not obvious（P〈0.05）; whole blood viscosity and erythrocyte aggregation index in the moxibustion along and against the meridian groups were significantly lower than those in the model group（P〈0.05）, while there were no statistically significant differences between the moxibustion along the meridian group and moxibustion against the meridian group（P〈0.05）. Conclusion： Moxibustion along and against the meridian both showed different degrees of improvement for hemorrheology and symptoms of blood stasis due to cold retention. Improvement for symptoms of blood stasis due to cold retention was better in the moxibustion along the meridian group than that in the moxibustion against the meridian group. The influence on rat hemorrheology showed no significant difference between moxibustion along the meridian and moxibustion against the meridian.
