Objective: To explore cytotoxicity of Synsepalum dulcificum(S. dulcificum) Daniell(Sapotaceae) on human colon cancer(HCT-116 and HT-29), human monocytic leukemia(THP-1) and normal(HDFn) cell lines, and its effect on t...Objective: To explore cytotoxicity of Synsepalum dulcificum(S. dulcificum) Daniell(Sapotaceae) on human colon cancer(HCT-116 and HT-29), human monocytic leukemia(THP-1) and normal(HDFn) cell lines, and its effect on the expression of early apoptotic genes, c-fos and c-jun. Methods: Leaf, stem and berry of S. dulcificum were separately extracted by using 2 solvents, 10% ethanol(EtOH) and 80% methanol(MeOH). PrestoB lue~? cell viability assay and q RT-PCR assay were conducted to examine the above objectives respectively. Results: Stem MeOH, stem EtOH, and berry EtOH extracts of S. dulcificum were cytotoxic to HCT-116 and HT-29 human colon cancer cells. For HCT-116, IC_(50) values of these 3 extracts were not significantly different(P>0.05) from that of the positive control bleomycin(IC_(50) of 33.57 μg/mL), while for HT-29, IC_(50) values of these 3 extracts were significantly lower(P<0.05) than that of bleomycin(IC_(50) of 25.24 μg/mL). None of the extracts were cytotoxic to the THP-1 monocytic leukemia cells and HDFn normal human dermal fibroblasts. For both HCT-116 and HT-29, these extracts significantly up-regulated(P<0.05) the expression of c-fos and c-jun compared to the untreated negative control. Conclusions: The results of this study suggest that cytotoxicity of stem MeOH, stem EtOH, and berry EtOH extracts of S. dulcificum on HCT-116 and HT-29 colon cancer cells is due to the induced apoptosis which is caused by the up-regulation of the expression of early apoptotic genes, c-fos and c-jun.展开更多
Objective: This study was undertaken to investigate the antihyperglycemic potential of miracle fruit(MF)as well as its hepatic safety as compared to aspartame in alloxan-induced diabetic mice.Methods: MF extracts were...Objective: This study was undertaken to investigate the antihyperglycemic potential of miracle fruit(MF)as well as its hepatic safety as compared to aspartame in alloxan-induced diabetic mice.Methods: MF extracts were prepared and screened for their phytochemical composition using highperformance liquid chromatography(HPLC). Total phenolic, flavonoid and tannin contents and antioxidant potential were also determined. Additionally, MF was evaluated for its sensory attributes. For in vivo work, MF ethanol extract at high(MFH: 500 mg/kg body weight [BW]) and low(MFL: 250 mg/kg BW) doses as well as aspartame were injected intraperitoneally into alloxan-induced diabetic mice.Blood glucose levels were determined following acute and subchronic treatment. At the end of the study,animals were sacrificed, serum was collected for biochemical analysis and liver tissues were obtained for histopathological examination.Results: MF ethanol extract contained more flavonoids and tannins, and had higher 1,1-diphenyl-1-picrylhydrazyl radical-scavenging activity(79.61%) compared to MF aqueous extract(P < 0.05). HPLC analysis of MF ethanol extract also revealed the presence of 10 antioxidants with quercetin comprising the major polyphenol. Additionally, sensory analysis of MF showed that its intake is effective in masking undesirable sourness. Subchronic administration of MFH proved amelioration of hyperglycemia in mice as compared to aspartame. Moreover, aspartame treatment significantly elevated(P < 0.05) the level of alanine aminotransferase and had destructive effects on the liver histopathology;however, hepatic architecture was restored by low and high doses of MF.Conclusion: MF is an effective antihyperglycemic with hepatoprotective properties that can be used as a healthier alternative sweetening agent in place of aspartame for sour beverages.展开更多
Objective:To determine the beneficial effects of the methanol extract of Synsepalum dulcificum on some biochemical parameters.Methods:In this study,rats were orally administered(gavage)with methanol extracts at doses ...Objective:To determine the beneficial effects of the methanol extract of Synsepalum dulcificum on some biochemical parameters.Methods:In this study,rats were orally administered(gavage)with methanol extracts at doses of 0 mg/kg(Group 1,as normal group),100 mg/kg(Group 2),200 mg/kg(Group 3)and 500 mg/kg(Group 4)body weight per day for 28 d.Results:Acute toxicity study showed that the methanol extract was not toxic to rats up to 5000 mg/kg.From the results,the 100 mg/kg doses of the extract significantly(P<0.05)reduced serum levels of bilirubin,low density lipoprotein,alanine aminotransferase and glucose after 14 d compared with those after 28 d.A significant difference(P<0.05)was observed in the malondialdehyde and serum protein concentration in Group 4 while glucose concentration decreased significantly(P<0.05)in Group 1 and Group 4 after 14 d compared with 28 d.The high density lipoprotein significantly increased(P<0.05)in Group 3.Conclusions:The fruit has no negative effect on some biochemical parameters in albino rats.展开更多
文摘Objective: To explore cytotoxicity of Synsepalum dulcificum(S. dulcificum) Daniell(Sapotaceae) on human colon cancer(HCT-116 and HT-29), human monocytic leukemia(THP-1) and normal(HDFn) cell lines, and its effect on the expression of early apoptotic genes, c-fos and c-jun. Methods: Leaf, stem and berry of S. dulcificum were separately extracted by using 2 solvents, 10% ethanol(EtOH) and 80% methanol(MeOH). PrestoB lue~? cell viability assay and q RT-PCR assay were conducted to examine the above objectives respectively. Results: Stem MeOH, stem EtOH, and berry EtOH extracts of S. dulcificum were cytotoxic to HCT-116 and HT-29 human colon cancer cells. For HCT-116, IC_(50) values of these 3 extracts were not significantly different(P>0.05) from that of the positive control bleomycin(IC_(50) of 33.57 μg/mL), while for HT-29, IC_(50) values of these 3 extracts were significantly lower(P<0.05) than that of bleomycin(IC_(50) of 25.24 μg/mL). None of the extracts were cytotoxic to the THP-1 monocytic leukemia cells and HDFn normal human dermal fibroblasts. For both HCT-116 and HT-29, these extracts significantly up-regulated(P<0.05) the expression of c-fos and c-jun compared to the untreated negative control. Conclusions: The results of this study suggest that cytotoxicity of stem MeOH, stem EtOH, and berry EtOH extracts of S. dulcificum on HCT-116 and HT-29 colon cancer cells is due to the induced apoptosis which is caused by the up-regulation of the expression of early apoptotic genes, c-fos and c-jun.
文摘Objective: This study was undertaken to investigate the antihyperglycemic potential of miracle fruit(MF)as well as its hepatic safety as compared to aspartame in alloxan-induced diabetic mice.Methods: MF extracts were prepared and screened for their phytochemical composition using highperformance liquid chromatography(HPLC). Total phenolic, flavonoid and tannin contents and antioxidant potential were also determined. Additionally, MF was evaluated for its sensory attributes. For in vivo work, MF ethanol extract at high(MFH: 500 mg/kg body weight [BW]) and low(MFL: 250 mg/kg BW) doses as well as aspartame were injected intraperitoneally into alloxan-induced diabetic mice.Blood glucose levels were determined following acute and subchronic treatment. At the end of the study,animals were sacrificed, serum was collected for biochemical analysis and liver tissues were obtained for histopathological examination.Results: MF ethanol extract contained more flavonoids and tannins, and had higher 1,1-diphenyl-1-picrylhydrazyl radical-scavenging activity(79.61%) compared to MF aqueous extract(P < 0.05). HPLC analysis of MF ethanol extract also revealed the presence of 10 antioxidants with quercetin comprising the major polyphenol. Additionally, sensory analysis of MF showed that its intake is effective in masking undesirable sourness. Subchronic administration of MFH proved amelioration of hyperglycemia in mice as compared to aspartame. Moreover, aspartame treatment significantly elevated(P < 0.05) the level of alanine aminotransferase and had destructive effects on the liver histopathology;however, hepatic architecture was restored by low and high doses of MF.Conclusion: MF is an effective antihyperglycemic with hepatoprotective properties that can be used as a healthier alternative sweetening agent in place of aspartame for sour beverages.
基金Support by Department of Biochemistry,Faculty of Biological Sciences,University of Nigeria,Nsukka,Enugu State,Nigeria(Grant No:2013).
文摘Objective:To determine the beneficial effects of the methanol extract of Synsepalum dulcificum on some biochemical parameters.Methods:In this study,rats were orally administered(gavage)with methanol extracts at doses of 0 mg/kg(Group 1,as normal group),100 mg/kg(Group 2),200 mg/kg(Group 3)and 500 mg/kg(Group 4)body weight per day for 28 d.Results:Acute toxicity study showed that the methanol extract was not toxic to rats up to 5000 mg/kg.From the results,the 100 mg/kg doses of the extract significantly(P<0.05)reduced serum levels of bilirubin,low density lipoprotein,alanine aminotransferase and glucose after 14 d compared with those after 28 d.A significant difference(P<0.05)was observed in the malondialdehyde and serum protein concentration in Group 4 while glucose concentration decreased significantly(P<0.05)in Group 1 and Group 4 after 14 d compared with 28 d.The high density lipoprotein significantly increased(P<0.05)in Group 3.Conclusions:The fruit has no negative effect on some biochemical parameters in albino rats.
