Synthesis of a Pair of Derivatives of N-Methylpyrrole Polyamide

A pair of derivatives of tetrameric N-methylpyrrole polyamide were synthesized in order to develop a new method for the study of interaction of the polyamide derivatives with DNA. Indole acetic acid and nicotinic acid were introduced to the polyamide in the synthesized compound, which showed an expected red shift in the UV spectrum. These compounds may function as a potential tool in the detection of the polyamide binding to DNA.

新型4-羟基香豆素衍生物的微波合成及其光谱性质

以4-羟基香豆素（a）为原料微波辐射合成了具有独特的生理活性和荧光性能的3,3′,3″,3′″-亚乙四基-4-羟基香豆素（b）、3,3′-苯亚甲基-双-4-羟基香豆素（c）和4-羟基香豆素-1,4-萘醌（d）系列4-羟基香豆素衍生物, 采用元素分析、红外光谱、核磁共振及质谱表征了产物的结构, 并对其紫外-可见吸收光谱及荧光光谱性质进行了研究, 探索了化合物的微观结构与其光学性能之间的关系. 研究结果表明, 具有“近平面”、大π共轭和对称型结构的化合物b具有较大的摩尔吸光系数及强荧光特性, 且浓度在0.50-1.50×10^-4 mol/L范围时, 其荧光强度随着浓度的降低而呈线性增加; 在pH=1.81-6.09时, 荧光强度随pH降低而减弱, 在pH 8.36~11.98时, 荧光强度随pH升高而减弱. 此外, 牛血清白蛋白（BSA）及脱氧核糖核酸（DNA）可与该化合物发生相互作用, 进而敏化增强该分子的内源荧光.