【目的】利用P2C可以定向进入卵巢以及Gal4蛋白可与UAS序列稳定结合的特点,在中华按蚊Anopheles sinensis中建立高效的非胚胎期外源DNA投递技术系统。【方法】注射P2C-Gal4-DsRed重组蛋白至吸血后20 h时的中华按蚊雌成蚊腹部,通过冰冻...【目的】利用P2C可以定向进入卵巢以及Gal4蛋白可与UAS序列稳定结合的特点,在中华按蚊Anopheles sinensis中建立高效的非胚胎期外源DNA投递技术系统。【方法】注射P2C-Gal4-DsRed重组蛋白至吸血后20 h时的中华按蚊雌成蚊腹部,通过冰冻切片荧光观察和Western blot检测分析重组蛋白P2C-Gal4-DsRed在卵巢中的投递效率;制备P2C-Gal4 DNA BINDING重组蛋白,构建包含12×UAS重复基序的转基因质粒和辅助质粒,通过电泳迁移实验分析重组蛋白P2C-Gal4 DNA BINDING和12×UAS重复基序间的体外结合;分别将体外孵育的P2C-Gal4 DNA BINDING+辅助质粒ITF36-12×UAS和P2C-Gal4 DNA BINDING+转基因质粒ITF2-12×UAS afm复合物注射入吸血后20 h时的中华按蚊雌成蚊腹部,于血餐后40 h时提取其卵巢组织DNA,并通过特异性引物PCR扩增和测序分析外源DNA在活体中的投递情况。【结果】100%注射P2C-Gal4-DsRed的中华按蚊雌成蚊卵巢在绿色滤光片下呈现明显的红色荧光,表明P2C-Gal4-DsRed重组蛋白能够被高效地导入雌成蚊卵巢中;P2C-Gal4 DNA BINDING重组蛋白能够与12×UAS重复基序以及含有该重复基序片段的质粒稳定结合;分别有91%和93%的注射了P2C-Gal4 DNA BINDING+ITF36-12×UAS和P2C-Gal4 DNA BINDING+ITF2-12×UAS afm的雌成蚊卵巢组织中能够检测到外源DNA片段。【结论】在中华按蚊中成功建立了基于P2C卵巢导向肽和Gal4-12×UAS重复基序结合特性的外源DNA投递技术体系;通过此技术平台能够便捷、快速和高效地实现质粒等DNA分子在中华按蚊卵巢中的投递,这为进一步简化转基因、过表达及基因敲入等遗传操作奠定了基础。展开更多
An Unmanned Aircraft System （UAS） is an aircraft or ground station that can be either remote controlled manually or is capable of flying autonomously under the guidance of pre-programmed Global Positioning System （...An Unmanned Aircraft System （UAS） is an aircraft or ground station that can be either remote controlled manually or is capable of flying autonomously under the guidance of pre-programmed Global Positioning System （GPS） waypoint flight plans or more complex onboard intelligent systems. The UAS aircrafts have recently found extensive applications in military reconnaissance and surveillance, homeland security, precision agriculture, fire monitoring and analysis, and other different kinds of aids needed in disasters. Through surveillance videos captured by a UAS digital imaging payload over the interest areas, the corresponding UAS missions can be conducted. In this paper, the authors present an effective method to detect and extract architectural buildings under rural environment from UAS video sequences. The SIFT points are chosen as image features. The planar homography is adopted as the motion model between different image frames. The proposed algorithm is tested on real UAS video data.展开更多
随着物联网与互联网融合的不断深化,感知层与应用层之间的互联互通要求不断提高。针对现有数据采集系统存在的规范性、扩展性和适应性不足的问题,文章提出一套基于对象连接与嵌入过程控制统一架构(object linking and embedding for pro...随着物联网与互联网融合的不断深化,感知层与应用层之间的互联互通要求不断提高。针对现有数据采集系统存在的规范性、扩展性和适应性不足的问题,文章提出一套基于对象连接与嵌入过程控制统一架构(object linking and embedding for process control unified architecture,OPC UA)协议的分布式数据采集处理系统架构。对数据采集处理系统各个模块进行功能解耦和架构重组,分别介绍该系统硬件和软件架构;针对发布订阅模式下负载的特异性,提出一种改进的适用于OPC UA分布式订阅的负载均衡算法;最后在某车企实例验证该系统架构。结果表明,基于OPC UA的分布式数据采集处理系统数据采集处理效果良好,证明了该系统架构的可行性及有效性。展开更多
文摘【目的】利用P2C可以定向进入卵巢以及Gal4蛋白可与UAS序列稳定结合的特点,在中华按蚊Anopheles sinensis中建立高效的非胚胎期外源DNA投递技术系统。【方法】注射P2C-Gal4-DsRed重组蛋白至吸血后20 h时的中华按蚊雌成蚊腹部,通过冰冻切片荧光观察和Western blot检测分析重组蛋白P2C-Gal4-DsRed在卵巢中的投递效率;制备P2C-Gal4 DNA BINDING重组蛋白,构建包含12×UAS重复基序的转基因质粒和辅助质粒,通过电泳迁移实验分析重组蛋白P2C-Gal4 DNA BINDING和12×UAS重复基序间的体外结合;分别将体外孵育的P2C-Gal4 DNA BINDING+辅助质粒ITF36-12×UAS和P2C-Gal4 DNA BINDING+转基因质粒ITF2-12×UAS afm复合物注射入吸血后20 h时的中华按蚊雌成蚊腹部,于血餐后40 h时提取其卵巢组织DNA,并通过特异性引物PCR扩增和测序分析外源DNA在活体中的投递情况。【结果】100%注射P2C-Gal4-DsRed的中华按蚊雌成蚊卵巢在绿色滤光片下呈现明显的红色荧光,表明P2C-Gal4-DsRed重组蛋白能够被高效地导入雌成蚊卵巢中;P2C-Gal4 DNA BINDING重组蛋白能够与12×UAS重复基序以及含有该重复基序片段的质粒稳定结合;分别有91%和93%的注射了P2C-Gal4 DNA BINDING+ITF36-12×UAS和P2C-Gal4 DNA BINDING+ITF2-12×UAS afm的雌成蚊卵巢组织中能够检测到外源DNA片段。【结论】在中华按蚊中成功建立了基于P2C卵巢导向肽和Gal4-12×UAS重复基序结合特性的外源DNA投递技术体系;通过此技术平台能够便捷、快速和高效地实现质粒等DNA分子在中华按蚊卵巢中的投递,这为进一步简化转基因、过表达及基因敲入等遗传操作奠定了基础。
文摘An Unmanned Aircraft System （UAS） is an aircraft or ground station that can be either remote controlled manually or is capable of flying autonomously under the guidance of pre-programmed Global Positioning System （GPS） waypoint flight plans or more complex onboard intelligent systems. The UAS aircrafts have recently found extensive applications in military reconnaissance and surveillance, homeland security, precision agriculture, fire monitoring and analysis, and other different kinds of aids needed in disasters. Through surveillance videos captured by a UAS digital imaging payload over the interest areas, the corresponding UAS missions can be conducted. In this paper, the authors present an effective method to detect and extract architectural buildings under rural environment from UAS video sequences. The SIFT points are chosen as image features. The planar homography is adopted as the motion model between different image frames. The proposed algorithm is tested on real UAS video data.
文摘随着物联网与互联网融合的不断深化,感知层与应用层之间的互联互通要求不断提高。针对现有数据采集系统存在的规范性、扩展性和适应性不足的问题,文章提出一套基于对象连接与嵌入过程控制统一架构(object linking and embedding for process control unified architecture,OPC UA)协议的分布式数据采集处理系统架构。对数据采集处理系统各个模块进行功能解耦和架构重组,分别介绍该系统硬件和软件架构;针对发布订阅模式下负载的特异性,提出一种改进的适用于OPC UA分布式订阅的负载均衡算法;最后在某车企实例验证该系统架构。结果表明,基于OPC UA的分布式数据采集处理系统数据采集处理效果良好,证明了该系统架构的可行性及有效性。
