[Objective] The experiment aimed to determine the optimum ISSR-PCR reaction system of Picea crassifolia kom. [Method] Picea crassifolia kom. was used as material to select and optimize influencing factors of ISSR-PCR ...[Objective] The experiment aimed to determine the optimum ISSR-PCR reaction system of Picea crassifolia kom. [Method] Picea crassifolia kom. was used as material to select and optimize influencing factors of ISSR-PCR such as Mg2+, dNTPs, Taq DNA polymerase, template DNA, primers, annealing temperature. [Result] The optimum ISSR-PCR reaction system in 20 μl reaction system was consisted of 1 μl 10×buffer, 1.5 mmol/L Mg2+, 0.2 mmol/L dNTPs, 1.0 U Taq DNA polymerase, 40 ng template DNA, 0.6 μmol/L primers. According to gradient test of annealing temperature in optimum ISSR-PCR reaction system of Picea crassifolia kom, it was found that the optimum annealing temperature of UBC 818 was 54.2 ℃ and the annealing temperature was different for different primers.[Conclusion]The construction of ISSR-PCR reaction system provided technical basis for classification of germplasm resources, construction of genetic map, gene mapping of Picea crassifolia kom. through using ISSR technology.展开更多
A project entitled‘Development of a Global High-resolution Marine Dynamic Environmental Forecasting System’has been funded by‘The Program on Marine Environmental Safety Guarantee’of The National Key Research and D...A project entitled‘Development of a Global High-resolution Marine Dynamic Environmental Forecasting System’has been funded by‘The Program on Marine Environmental Safety Guarantee’of The National Key Research and Development Program of China.This project will accomplish its objectives through basic theoretical research,model development and expansion,and system establishment and application,with a focus on four key issues separated into nine tasks.A series of research achievements have already been obtained,including datasets,observations,theories,and model results.展开更多
文摘[Objective] The experiment aimed to determine the optimum ISSR-PCR reaction system of Picea crassifolia kom. [Method] Picea crassifolia kom. was used as material to select and optimize influencing factors of ISSR-PCR such as Mg2+, dNTPs, Taq DNA polymerase, template DNA, primers, annealing temperature. [Result] The optimum ISSR-PCR reaction system in 20 μl reaction system was consisted of 1 μl 10×buffer, 1.5 mmol/L Mg2+, 0.2 mmol/L dNTPs, 1.0 U Taq DNA polymerase, 40 ng template DNA, 0.6 μmol/L primers. According to gradient test of annealing temperature in optimum ISSR-PCR reaction system of Picea crassifolia kom, it was found that the optimum annealing temperature of UBC 818 was 54.2 ℃ and the annealing temperature was different for different primers.[Conclusion]The construction of ISSR-PCR reaction system provided technical basis for classification of germplasm resources, construction of genetic map, gene mapping of Picea crassifolia kom. through using ISSR technology.
基金funded by "The Program on Marine Environmental Safety Guarantee" of "The National Key Research and Development Program of China"[grant number2016YFC1401409]
文摘A project entitled‘Development of a Global High-resolution Marine Dynamic Environmental Forecasting System’has been funded by‘The Program on Marine Environmental Safety Guarantee’of The National Key Research and Development Program of China.This project will accomplish its objectives through basic theoretical research,model development and expansion,and system establishment and application,with a focus on four key issues separated into nine tasks.A series of research achievements have already been obtained,including datasets,observations,theories,and model results.
