A new stem rot disease is found to occur naturally on Arabidopsis plants in greenhouses of Fuzhou, China. In order to identify its pathogen, we conducted a series of fungal isolation and purification, plant reinoculat...A new stem rot disease is found to occur naturally on Arabidopsis plants in greenhouses of Fuzhou, China. In order to identify its pathogen, we conducted a series of fungal isolation and purification, plant reinoculation, and ascus and ascospore induction from the sclerotia. The isolate caused typical water-soaked lesions after reinoculation and produced sclerotia both on Arabidopsis plants and culture medium plates, and the sclerotia could be induced to produce discal apothecia and 8 binucleate ascospores per ascus. These disease symptom and fungal morphology data revealed that the fungus Sclerotinia sclerotiorum (Lib.) de Bary was the pathogen for Arabidopsis stem rot. To confirm this, we further amplified its large subunit ribosomal DNA (LSU rDNA) by polymerase chain reaction (PCR), and compared the sequence with the known LSU rDNA sequences in GenBank. The results show that the sequence shares the highest identities with the LSU rDNAs of different S. sclerotiorum strains. Taking all these data together, we concluded that the fungus that caused the Arabidopsis stem rot is S. sclerotiorum (Lib.) de Bary. This is the first report that Arabidopsis is naturally infected by S. sclerotiorum.展开更多
The design of landing gear is complicated due to the numerous considered elements.And the initial elements related to each other can also be influenced by different factors.Landing gear design often involves a very la...The design of landing gear is complicated due to the numerous considered elements.And the initial elements related to each other can also be influenced by different factors.Landing gear design often involves a very large variety of configurations,especially in the conceptual design phase.However,traditional method costs more time to complete the whole procedure for suitable configurations of landing gears.Therefore,the parametric modeling of component library for landing gear based on computer aided three-dimensional interface application/component application architecutre(CATIA/CAA)is proposed.According to the analysis of the characteristics of landing gear components,a method is presented to extract the primary parameters of landing gear components so that a systematic classification can be established.Further,the related theories and methods,including receiving geometrical parameters of the components and updating the parametrical model,displaying the component parts,are also illustrated.Finally,the development technology for component library is explained.The proposed modeling method can improve the efficiency of the whole design cycle for landing gear.展开更多
基金the National Natural Science Foundation of China (No. 30471178)the Ministry of Agriculture of China (No.nyhyzx07-054)
文摘A new stem rot disease is found to occur naturally on Arabidopsis plants in greenhouses of Fuzhou, China. In order to identify its pathogen, we conducted a series of fungal isolation and purification, plant reinoculation, and ascus and ascospore induction from the sclerotia. The isolate caused typical water-soaked lesions after reinoculation and produced sclerotia both on Arabidopsis plants and culture medium plates, and the sclerotia could be induced to produce discal apothecia and 8 binucleate ascospores per ascus. These disease symptom and fungal morphology data revealed that the fungus Sclerotinia sclerotiorum (Lib.) de Bary was the pathogen for Arabidopsis stem rot. To confirm this, we further amplified its large subunit ribosomal DNA (LSU rDNA) by polymerase chain reaction (PCR), and compared the sequence with the known LSU rDNA sequences in GenBank. The results show that the sequence shares the highest identities with the LSU rDNAs of different S. sclerotiorum strains. Taking all these data together, we concluded that the fungus that caused the Arabidopsis stem rot is S. sclerotiorum (Lib.) de Bary. This is the first report that Arabidopsis is naturally infected by S. sclerotiorum.
基金Supported by the National Natural Science Foundation of China(51075203,51105197)the Nanjing University of Aeronautics and Astronautics Research Funding(NS2010023)
文摘The design of landing gear is complicated due to the numerous considered elements.And the initial elements related to each other can also be influenced by different factors.Landing gear design often involves a very large variety of configurations,especially in the conceptual design phase.However,traditional method costs more time to complete the whole procedure for suitable configurations of landing gears.Therefore,the parametric modeling of component library for landing gear based on computer aided three-dimensional interface application/component application architecutre(CATIA/CAA)is proposed.According to the analysis of the characteristics of landing gear components,a method is presented to extract the primary parameters of landing gear components so that a systematic classification can be established.Further,the related theories and methods,including receiving geometrical parameters of the components and updating the parametrical model,displaying the component parts,are also illustrated.Finally,the development technology for component library is explained.The proposed modeling method can improve the efficiency of the whole design cycle for landing gear.
