Objective:To investigate the clinical diagnostic significance of peripheral blood T-cell test(T-spot test)for tuberculosis(TB)infection combined with erythrocyte sedimentation rate(ESR)in pulmonary TB.Methods:41 patie...Objective:To investigate the clinical diagnostic significance of peripheral blood T-cell test(T-spot test)for tuberculosis(TB)infection combined with erythrocyte sedimentation rate(ESR)in pulmonary TB.Methods:41 patients with a clinical diagnosis of TB during hospitalization from January 2020 to April 2023 in our hospital were selected as the experimental group,and 45 patients without TB(bronchopneumonia patients)were selected as the control group.The diagnostic specificity,sensitivity,and accuracy of the T-spot TB test,ESR test,and the combined test of the two were calculated respectively.Results:The sensitivity,specificity,and accuracy of the T-spot TB test combined with ESR for the diagnosis of TB in the experimental group were significantly higher than the individual results of the T-spot TB test and ESR test alone(P<0.05).Conclusion:The T-spot TB test combined with the ESR test for TB diagnosis has greater clinical value than carrying out the tests individually.展开更多
Enhancement of the Human Immunodeficiency Virus (HIV) specific cytotoxic T-cells mechanisms in an HIV-1 and Mycobacterium tuberculosis (Mtb) co-infected individual seems to improve the clinical picture of an individua...Enhancement of the Human Immunodeficiency Virus (HIV) specific cytotoxic T-cells mechanisms in an HIV-1 and Mycobacterium tuberculosis (Mtb) co-infected individual seems to improve the clinical picture of an individual by reducing Acquired Immuno Deficiency Syndrome (AIDS) state progression rate. In this paper, we develop a system of deterministic differential equations representing the immune cells involved in an HIV-1 and Mtb co-infected individual. Results show that although the non-lytic arm of the HIV-1 cytotoxic T-cells affects the co-infection dynamics more than the lytic factors, a combination of both factors results in a more positive reduced progression to the AIDS state. This is due to the increased protection of the CD4<sup>+</sup> T-cells by the CTL mechanisms by further reducing infections and replications by the HIV. Thus, HIV-1 specific CTLs mechanisms’ involvement is here recommended to be part of a solution to the HIV and Mtb co-infection problems.展开更多
Objective: To investigate the correlation of CD4+T cell and tuberculosis test in AIDS patients with different types of tuberculosis. Methods: A total of 127 cases of AIDS complicated with tuberculosis were selected fr...Objective: To investigate the correlation of CD4+T cell and tuberculosis test in AIDS patients with different types of tuberculosis. Methods: A total of 127 cases of AIDS complicated with tuberculosis were selected from our hospital between June 2014 and June 2017 as observation group;50 cases with non-tuberculosis respiratory system who were admitted to our hospital during the same period were selected as control group. The positive rate of TB was examined by T.spotTB test and TST test, and the level of CD4+T lymphocyte was measured by flow cytometry. Results: The positive rate of the observed group After T.spotTB test and TST test was higher than the control group;observation group CD4+T lymphocyte level of 0-100/mm3 was significantly higher than that of the control group;different types of tuberculosis with AIDS showed no significant difference between the T.spotTB test and the TST test in terms of positive rate;There was also no significant difference between the different types of tuberculosis with HIV CD4+T lymphocyte level;the levels of 100-200/mm3 and >200/mm3 CD4+T lymphocytes and T.spotTB test positive and TST positive test were negatively correlated, while the levels of CD4+T lymphocyte 0-100/mm3 and positive T.spotTB test and TST test were positively correlated. Conclusions: the lower level of CD4+T cells in AIDS patients with different types of tuberculosis is positively related to the positive T.spotTB test and TST test.展开更多
目的探讨结核分枝杆菌感染T细胞斑点试验（spot test of T cells infected with Mycobacterium tuberculosis,TSPOT.TB）评分在多系统结核感染诊断中的应用价值。方法回顾性纳入2013年10月至2014年9月在郑州大学第一附属医院住院并确诊...目的探讨结核分枝杆菌感染T细胞斑点试验（spot test of T cells infected with Mycobacterium tuberculosis,TSPOT.TB）评分在多系统结核感染诊断中的应用价值。方法回顾性纳入2013年10月至2014年9月在郑州大学第一附属医院住院并确诊为结核感染的530例患者,所有患者均应用T-SPOT.TB试剂盒检测。分别将外周血中经6 k D早期分泌靶抗原（early secretory antigenic target 6,ESAT-6）和10 k D培养滤过蛋白（culture filtrate protein 10,CFP-10）刺激后T细胞斑点形成数记为其A值、B值。根据病变侵及范围分为单系统结核感染组和多系统结核感染组,分析两组间阳性检出率及两组A值、B值的差异。结果 T-SPOT.TB诊断结核感染的总敏感性为87.7%（465/530）。多系统结核感染组的阳性检出率94.1%（143/152）高于单系统结核感染组的阳性检出率85.1%（322/378）。多系统感染组的A值（202.32±4.57）/10~6PBMCs及B值（207.20±4.98）/10~6PBMCs均显著高于单系统感染组的A值（35.51±2.38）/10~6PBMCs及B值（38.48±1.91）/10~6PBMCs,差异均具有统计学意义（t_A=5.33;t_B=4.48;P〈0.001）。结论 T-SPOT.TB在诊断单系统及多系统结核感染中具有较高的敏感性,其值越高,提示多系统结核感染的可能性越大。展开更多
文摘Objective:To investigate the clinical diagnostic significance of peripheral blood T-cell test(T-spot test)for tuberculosis(TB)infection combined with erythrocyte sedimentation rate(ESR)in pulmonary TB.Methods:41 patients with a clinical diagnosis of TB during hospitalization from January 2020 to April 2023 in our hospital were selected as the experimental group,and 45 patients without TB(bronchopneumonia patients)were selected as the control group.The diagnostic specificity,sensitivity,and accuracy of the T-spot TB test,ESR test,and the combined test of the two were calculated respectively.Results:The sensitivity,specificity,and accuracy of the T-spot TB test combined with ESR for the diagnosis of TB in the experimental group were significantly higher than the individual results of the T-spot TB test and ESR test alone(P<0.05).Conclusion:The T-spot TB test combined with the ESR test for TB diagnosis has greater clinical value than carrying out the tests individually.
文摘Enhancement of the Human Immunodeficiency Virus (HIV) specific cytotoxic T-cells mechanisms in an HIV-1 and Mycobacterium tuberculosis (Mtb) co-infected individual seems to improve the clinical picture of an individual by reducing Acquired Immuno Deficiency Syndrome (AIDS) state progression rate. In this paper, we develop a system of deterministic differential equations representing the immune cells involved in an HIV-1 and Mtb co-infected individual. Results show that although the non-lytic arm of the HIV-1 cytotoxic T-cells affects the co-infection dynamics more than the lytic factors, a combination of both factors results in a more positive reduced progression to the AIDS state. This is due to the increased protection of the CD4<sup>+</sup> T-cells by the CTL mechanisms by further reducing infections and replications by the HIV. Thus, HIV-1 specific CTLs mechanisms’ involvement is here recommended to be part of a solution to the HIV and Mtb co-infection problems.
文摘Objective: To investigate the correlation of CD4+T cell and tuberculosis test in AIDS patients with different types of tuberculosis. Methods: A total of 127 cases of AIDS complicated with tuberculosis were selected from our hospital between June 2014 and June 2017 as observation group;50 cases with non-tuberculosis respiratory system who were admitted to our hospital during the same period were selected as control group. The positive rate of TB was examined by T.spotTB test and TST test, and the level of CD4+T lymphocyte was measured by flow cytometry. Results: The positive rate of the observed group After T.spotTB test and TST test was higher than the control group;observation group CD4+T lymphocyte level of 0-100/mm3 was significantly higher than that of the control group;different types of tuberculosis with AIDS showed no significant difference between the T.spotTB test and the TST test in terms of positive rate;There was also no significant difference between the different types of tuberculosis with HIV CD4+T lymphocyte level;the levels of 100-200/mm3 and >200/mm3 CD4+T lymphocytes and T.spotTB test positive and TST positive test were negatively correlated, while the levels of CD4+T lymphocyte 0-100/mm3 and positive T.spotTB test and TST test were positively correlated. Conclusions: the lower level of CD4+T cells in AIDS patients with different types of tuberculosis is positively related to the positive T.spotTB test and TST test.
文摘目的探讨结核分枝杆菌感染T细胞斑点试验（spot test of T cells infected with Mycobacterium tuberculosis,TSPOT.TB）评分在多系统结核感染诊断中的应用价值。方法回顾性纳入2013年10月至2014年9月在郑州大学第一附属医院住院并确诊为结核感染的530例患者,所有患者均应用T-SPOT.TB试剂盒检测。分别将外周血中经6 k D早期分泌靶抗原（early secretory antigenic target 6,ESAT-6）和10 k D培养滤过蛋白（culture filtrate protein 10,CFP-10）刺激后T细胞斑点形成数记为其A值、B值。根据病变侵及范围分为单系统结核感染组和多系统结核感染组,分析两组间阳性检出率及两组A值、B值的差异。结果 T-SPOT.TB诊断结核感染的总敏感性为87.7%（465/530）。多系统结核感染组的阳性检出率94.1%（143/152）高于单系统结核感染组的阳性检出率85.1%（322/378）。多系统感染组的A值（202.32±4.57）/10~6PBMCs及B值（207.20±4.98）/10~6PBMCs均显著高于单系统感染组的A值（35.51±2.38）/10~6PBMCs及B值（38.48±1.91）/10~6PBMCs,差异均具有统计学意义（t_A=5.33;t_B=4.48;P〈0.001）。结论 T-SPOT.TB在诊断单系统及多系统结核感染中具有较高的敏感性,其值越高,提示多系统结核感染的可能性越大。
