Study on Lymphocyte Activation and Proliferation Induced by Anti-CD3 McAb

T cell activation and proliferation via CD3-TCR complex were investigated by lymphocyte DNA synthesis in vitro.Several interfering factors were also discussed.The result indicated that lymphocyte activation and proliferation are calciumdependent.A rise of cytoplasmic free Ca2+ quickly following activation with CD3 McAb is mainly due to intracellular mobilization of Ca2+,while lymphocyte proliferation needs both intracellular mobilization of Ca2+ as well as influx of extracellular Ca2+, It was confirmed that CTX sensitive G protein plays a role in regulating T cell proliferation by pretreatment with CTX suppressing lymphocyte H-TdR incorporation obviously.PLC and PKC inhibitor neomycin and P.S.S could also decrease T cell proliferation.

淋巴细胞[Ca^(2+)]i变化在小儿反复呼吸道感染发病机制中作用的探讨

目的：探讨反复呼吸道感染（ＲＲＴＩ）患儿Ｔ淋巴细胞增殖及胞浆游离钙浓度［Ｃａ２＋］ｉ的变化，及其在发病机制中的作用。方法：选取ＲＲＴＩ患儿３０例，健康儿童３１例为对照组，测定外周血单个核细胞（ＰＢＭＣ）中Ｔ细胞增殖、Ｔ细胞活化及未活化时［Ｃａ２＋］ｉ的变化。结果：ＲＲＴＩ与对照组相比，Ｔ细胞增殖明显降低（Ｐ＜００１）；Ｔ细胞未活化时两组间［Ｃａ２＋］ｉ无明显差异（Ｐ＞００５）；当Ｔ细胞活化时，ＲＲＴＩ组［Ｃａ２＋］ｉ明显低于对照组（Ｐ＜００１）。结论：ＲＲＴＩ患儿Ｔ细胞增殖能力降低，细胞免疫功能低下，这是患儿易发生反复感染的原因之一。Ｔ细胞活化过程中Ｃａ２＋动员存在一定程度异常，这是其增殖能力降低的原因之一。

淋巴细胞[Ca^(++)]i变化在小儿反复呼吸道感染发病机制中作用的探讨:附30例报告

为了探讨反复呼吸道感染(RRTI)患儿T淋巴细胞增殖及胞浆游离钙浓度([Ca^(++)]i)的变化,及其在发病机制中的作用,取RRTI患儿30例,健康儿童31例为对照组,测定外周血单个核细胞(PBMC)中T细胞增殖、T细胞活化及未活化时[Ca^(++)]i的变化。结果RRTI组与对照组相比,T细胞增殖明显降低(P<0.01);T细胞未活化时两组间[Ca^(++)]i无明显差异(P>0.05);当T细胞活化时,RRTI组[Ca^(++)]i明显低于对照组(P<0.01)。提示:RRTI患儿T细胞增殖能力降低,细胞免疫功能低下,这是患儿易发生反复感染的原因之一,T细胞活化过程中钙动员存在一定程度异常,这是其细胞增殖能力降低的原因之一。