Background T-lymphoma and metastasis gene 1 (Tiaml) produces a guanine nucleotide exchange factor (GNEF) that regulates guanosine triphosphatase, which transforms guanosine diphosphate to guanosine triphosphate. R...Background T-lymphoma and metastasis gene 1 (Tiaml) produces a guanine nucleotide exchange factor (GNEF) that regulates guanosine triphosphatase, which transforms guanosine diphosphate to guanosine triphosphate. Recently published data indicate that Tiaml was associated with gastric cancer. The aim of this study was to investigate biological effects and potential mechanisms of Tiara1 in gastric carcinoma. Methods We analyzed the expression of Tiaml in 114 pair-matched gastric neoplastic and adjacent non-neoplastic tissues by quantitative real-time PCR. We investigated Tiaml expression and its prognostic value for gastric cancer. Furthermore, the functions of Tiaml over-expression were analyzed with stable-expression Tiara1 plasmid in human gastric cancer cell lines. Results Tiaml expression was significantly associated with cell differentiation and lymphatic metastasis; expression of Tiaml mRNA was up-regulated in gastric cancer compared to pair-matched adjacent non-tumor tissues. Analyses of surgical tissue samples and 5-year survival of gastric cancer patients showed that those with strong Tiaml expression had significantly shorter overall survival time than those with negative Tiaml expression. Ectopic expression of Tiaml promoted cell growth, migration and invasion of gastric cancer cells in vitro. Conclusions In gastric cancer cells, Tiaml affects multiple properties associated with acquisition of the metastatic phenotype, and may be a marker of gastric cancer progression and metastasis in a subset of cancer.展开更多
基金This study was supported by a grant from the Natural Science Foundation of Shandong Province of China (Nos. ZR2011HM041, Y2007C102, ZR2011HM076), Key Research Project from Shandong Science and Technology Commission (Nos. 2011GGH21844, 2007H2071), and the the National Youthful Science Foundation of China (No. 81101858).
文摘Background T-lymphoma and metastasis gene 1 (Tiaml) produces a guanine nucleotide exchange factor (GNEF) that regulates guanosine triphosphatase, which transforms guanosine diphosphate to guanosine triphosphate. Recently published data indicate that Tiaml was associated with gastric cancer. The aim of this study was to investigate biological effects and potential mechanisms of Tiara1 in gastric carcinoma. Methods We analyzed the expression of Tiaml in 114 pair-matched gastric neoplastic and adjacent non-neoplastic tissues by quantitative real-time PCR. We investigated Tiaml expression and its prognostic value for gastric cancer. Furthermore, the functions of Tiaml over-expression were analyzed with stable-expression Tiara1 plasmid in human gastric cancer cell lines. Results Tiaml expression was significantly associated with cell differentiation and lymphatic metastasis; expression of Tiaml mRNA was up-regulated in gastric cancer compared to pair-matched adjacent non-tumor tissues. Analyses of surgical tissue samples and 5-year survival of gastric cancer patients showed that those with strong Tiaml expression had significantly shorter overall survival time than those with negative Tiaml expression. Ectopic expression of Tiaml promoted cell growth, migration and invasion of gastric cancer cells in vitro. Conclusions In gastric cancer cells, Tiaml affects multiple properties associated with acquisition of the metastatic phenotype, and may be a marker of gastric cancer progression and metastasis in a subset of cancer.