目的检测腹主动脉瘤(AAA)病人血清和组织中Notch1和T细胞激活分泌调节因子(reduced upon activation mormal T expression and secreted,RANTES)的表达,分析其意义。方法2019年1月~2021年1月间我院收治AAA病人41例为AAA组,同期健康志愿...目的检测腹主动脉瘤(AAA)病人血清和组织中Notch1和T细胞激活分泌调节因子(reduced upon activation mormal T expression and secreted,RANTES)的表达,分析其意义。方法2019年1月~2021年1月间我院收治AAA病人41例为AAA组,同期健康志愿者41例为健康组。通过RT-qPCR和ELISA检测血清中Notch1 mRNA和RANTES的水平。通过Western blot检测AAA组织中Notch1和RANTES蛋白表达水平。通过Pearson检测连续变量的相关性。结果AAA病人血清中Notch1 mRNA和RANTES水平分别为3.85±0.62和(58.46±5.59)μg/L健康组分别为1.04±0.23和(21.07±3.25)μg/L,两组比较差异有统计学意义(P<0.05)。AAA组织中的Notch1和RANTES蛋白表达水平分别为3.24±0.65和3.41±0.72,正常腹主动脉组织分别为1.08±0.21和1.12±0.23,两组比较差异有统计学意义(P<0.05)。AAA病人组织Notch1蛋白水平和血清Notch1 mRNA水平正相关(r=0.485,P=0.025);组织中RANTES蛋白水平与血清RAN-TES浓度正相关(r=0.506,P=0.016)。在AAA病人组织和血清中,Notch1和RANTES表达均呈正相关(r_(血清)=0.515,P=0.014;r_(组织)=0.537,P=0.008)。结论AAA病人血清和组织中的Notch1和RANTES均过表达,且二者正相关。展开更多
目的探讨血清白介素6(IL-6)和T细胞激活分泌调节因子(RANTES)在腹主动脉瘤的表达及其相关性。方法选取自2016年5月—2017年12月来我院就诊并确诊为腹主动脉瘤的患者40例作为研究组,同期门诊查体健康者40例作为对照组。采用酶联免疫法检...目的探讨血清白介素6(IL-6)和T细胞激活分泌调节因子(RANTES)在腹主动脉瘤的表达及其相关性。方法选取自2016年5月—2017年12月来我院就诊并确诊为腹主动脉瘤的患者40例作为研究组,同期门诊查体健康者40例作为对照组。采用酶联免疫法检测血清IL-6与RANTES水平、应用荧光定量PCR检测IL-6和RANTES m RNA的表达,分析血清IL-6与RANTES与临床病理参数的关系以及IL-6与RANTES相关性。结果两组血清IL-6浓度比较,差异有统计学意义(P<0.01);两组血清RANTES浓度比较,差异有统计学意义(P<0.01)。两组IL-6、RANTES m RNA相对表达比较,差异有统计学意义(P<0.01)。腹主动脉瘤中动脉瘤直径≥5.0 cm和有心血管疾病史患者IL-6、RANTES的表达高于动脉瘤直径<5.0 cm和无心血管疾病者,RANTES m RNA及浓度和IL-16呈正相关关系(r=0.521 6,P<0.005;r=0.6021,P<0.005)。结论腹主动脉瘤患者血清IL-6和RANTES水平显著升高,且二者呈正相关性。展开更多
Hepatocellular carcinoma (HCC) has a poor prognosis with limited therapeutic o ptions. We propose that local immune responses in patients with HCC are held in check by tumorinfiltrating CD4+CD25+T-regulatory lymphocyt...Hepatocellular carcinoma (HCC) has a poor prognosis with limited therapeutic o ptions. We propose that local immune responses in patients with HCC are held in check by tumorinfiltrating CD4+CD25+T-regulatory lymphocytes(Treg cells),which suppress the activity and proliferation of effector CD4+and CD8 +T cells. The phenotype and cell cycle status of tumor-infiltrating lymphocyte s (TILs) in HCC were analyzed via immunohistochemistry of sections from patients undergoing surgery for HCC and via flow cytometry of peripheral blood mononucle ar cells and TILs isolated from patients with HCC.Circulating and tumor-infiltr ating T-cell function and activation status were assessed via proliferation and flow cytometry. More than 96%of TILs were quiescent as measured via Mcm-2 or Ki-67 expression, while less than 10%of CD8+T cells expressed perform or gran zyme B. CD4+CD25+Treg cells comprised 8.7%(1.4-13.8) of TILs and always exce ed- ed the proportion in distant nontumor tissue (2.4%[1.5-5.6]; P = .014). Treg cells isolated from HCC suppressed proliferation of autologous circulating CD4 +CD25-cells and perforin expression and proliferation of autologous CD8+T cel ls. The proportion of circulating Treg cells in patients with HCC was similar in healthy controls(7.2%[1.2-23.3] and 9.2%[1.6-30.2], respectively), but the proportion of circulating Treg cells that were also transforming growth factor β1+was elevated in HCC compared with controls(55.5%[8.2-73.9] and 2.0%[0-4 .9], respectively; P = .003). In conclusion, TILs are compromised and contain a subpopulation of suppressive CD4+ CD25+Foxp3+Treg cells. Functional deletion of tumor-infiltrating Treg cells could enhance tumor specific immunot- herapy.展开更多
目的:观察雌激素(E2)诱导的大鼠慢性非细菌性前列腺炎(chronic nonbacterial prostatitis,CNP)中信号转导子及转录激活子1(signal transducer and activator of transcription 1,STAT-1)的激活和调节活化正常T细胞表达及分泌因子(regula...目的:观察雌激素(E2)诱导的大鼠慢性非细菌性前列腺炎(chronic nonbacterial prostatitis,CNP)中信号转导子及转录激活子1(signal transducer and activator of transcription 1,STAT-1)的激活和调节活化正常T细胞表达及分泌因子(regulated on activation,normal T-cell expressed and secreted,RANTES)的表达,探讨雌激素诱导炎症形成的机制。方法:将80只老年雄性SD大鼠随机分为对照组、去势组、去势+E2组和去势+E2+AG490组,每组20只。苏木素-伊红(HE)染色观察大鼠前列腺组织病理改变。Western blotting法测定STAT-1及p-STAT-1蛋白水平。RT-PCR和免疫组化SP法分别检测RANTES mRNA和蛋白表达水平,并分析p-STAT-1与RANTES表达水平的相关性。结果:去势+E2组前列腺组织呈明显炎症表现。去势+E2组中STAT-1及p-STAT-1蛋白表达明显高于对照组及去势组(P<0.01),RANTES mRNA和蛋白表达也显著增高(P<0.01)。去势+E2+AG490组中STAT-1及p-STAT-1蛋白、RANTES mRNA和蛋白表达水平较去势+E2组明显降低(P<0.01)。大鼠前列腺组织中p-STAT-1表达水平与RANTES mRNA转录水平呈正相关(r=0.735,P<0.05),RANTES表达水平与RANTESmRNA转录水平亦呈正相关(r=0.694,P<0.05)。结论:雌激素诱导CNP的形成可能与调节转录因子STAT-1、促进RANTES分子的表达及炎症反应有关。展开更多
文摘目的检测腹主动脉瘤(AAA)病人血清和组织中Notch1和T细胞激活分泌调节因子(reduced upon activation mormal T expression and secreted,RANTES)的表达,分析其意义。方法2019年1月~2021年1月间我院收治AAA病人41例为AAA组,同期健康志愿者41例为健康组。通过RT-qPCR和ELISA检测血清中Notch1 mRNA和RANTES的水平。通过Western blot检测AAA组织中Notch1和RANTES蛋白表达水平。通过Pearson检测连续变量的相关性。结果AAA病人血清中Notch1 mRNA和RANTES水平分别为3.85±0.62和(58.46±5.59)μg/L健康组分别为1.04±0.23和(21.07±3.25)μg/L,两组比较差异有统计学意义(P<0.05)。AAA组织中的Notch1和RANTES蛋白表达水平分别为3.24±0.65和3.41±0.72,正常腹主动脉组织分别为1.08±0.21和1.12±0.23,两组比较差异有统计学意义(P<0.05)。AAA病人组织Notch1蛋白水平和血清Notch1 mRNA水平正相关(r=0.485,P=0.025);组织中RANTES蛋白水平与血清RAN-TES浓度正相关(r=0.506,P=0.016)。在AAA病人组织和血清中,Notch1和RANTES表达均呈正相关(r_(血清)=0.515,P=0.014;r_(组织)=0.537,P=0.008)。结论AAA病人血清和组织中的Notch1和RANTES均过表达,且二者正相关。
文摘目的探讨血清白介素6(IL-6)和T细胞激活分泌调节因子(RANTES)在腹主动脉瘤的表达及其相关性。方法选取自2016年5月—2017年12月来我院就诊并确诊为腹主动脉瘤的患者40例作为研究组,同期门诊查体健康者40例作为对照组。采用酶联免疫法检测血清IL-6与RANTES水平、应用荧光定量PCR检测IL-6和RANTES m RNA的表达,分析血清IL-6与RANTES与临床病理参数的关系以及IL-6与RANTES相关性。结果两组血清IL-6浓度比较,差异有统计学意义(P<0.01);两组血清RANTES浓度比较,差异有统计学意义(P<0.01)。两组IL-6、RANTES m RNA相对表达比较,差异有统计学意义(P<0.01)。腹主动脉瘤中动脉瘤直径≥5.0 cm和有心血管疾病史患者IL-6、RANTES的表达高于动脉瘤直径<5.0 cm和无心血管疾病者,RANTES m RNA及浓度和IL-16呈正相关关系(r=0.521 6,P<0.005;r=0.6021,P<0.005)。结论腹主动脉瘤患者血清IL-6和RANTES水平显著升高,且二者呈正相关性。
文摘Hepatocellular carcinoma (HCC) has a poor prognosis with limited therapeutic o ptions. We propose that local immune responses in patients with HCC are held in check by tumorinfiltrating CD4+CD25+T-regulatory lymphocytes(Treg cells),which suppress the activity and proliferation of effector CD4+and CD8 +T cells. The phenotype and cell cycle status of tumor-infiltrating lymphocyte s (TILs) in HCC were analyzed via immunohistochemistry of sections from patients undergoing surgery for HCC and via flow cytometry of peripheral blood mononucle ar cells and TILs isolated from patients with HCC.Circulating and tumor-infiltr ating T-cell function and activation status were assessed via proliferation and flow cytometry. More than 96%of TILs were quiescent as measured via Mcm-2 or Ki-67 expression, while less than 10%of CD8+T cells expressed perform or gran zyme B. CD4+CD25+Treg cells comprised 8.7%(1.4-13.8) of TILs and always exce ed- ed the proportion in distant nontumor tissue (2.4%[1.5-5.6]; P = .014). Treg cells isolated from HCC suppressed proliferation of autologous circulating CD4 +CD25-cells and perforin expression and proliferation of autologous CD8+T cel ls. The proportion of circulating Treg cells in patients with HCC was similar in healthy controls(7.2%[1.2-23.3] and 9.2%[1.6-30.2], respectively), but the proportion of circulating Treg cells that were also transforming growth factor β1+was elevated in HCC compared with controls(55.5%[8.2-73.9] and 2.0%[0-4 .9], respectively; P = .003). In conclusion, TILs are compromised and contain a subpopulation of suppressive CD4+ CD25+Foxp3+Treg cells. Functional deletion of tumor-infiltrating Treg cells could enhance tumor specific immunot- herapy.
文摘目的:观察雌激素(E2)诱导的大鼠慢性非细菌性前列腺炎(chronic nonbacterial prostatitis,CNP)中信号转导子及转录激活子1(signal transducer and activator of transcription 1,STAT-1)的激活和调节活化正常T细胞表达及分泌因子(regulated on activation,normal T-cell expressed and secreted,RANTES)的表达,探讨雌激素诱导炎症形成的机制。方法:将80只老年雄性SD大鼠随机分为对照组、去势组、去势+E2组和去势+E2+AG490组,每组20只。苏木素-伊红(HE)染色观察大鼠前列腺组织病理改变。Western blotting法测定STAT-1及p-STAT-1蛋白水平。RT-PCR和免疫组化SP法分别检测RANTES mRNA和蛋白表达水平,并分析p-STAT-1与RANTES表达水平的相关性。结果:去势+E2组前列腺组织呈明显炎症表现。去势+E2组中STAT-1及p-STAT-1蛋白表达明显高于对照组及去势组(P<0.01),RANTES mRNA和蛋白表达也显著增高(P<0.01)。去势+E2+AG490组中STAT-1及p-STAT-1蛋白、RANTES mRNA和蛋白表达水平较去势+E2组明显降低(P<0.01)。大鼠前列腺组织中p-STAT-1表达水平与RANTES mRNA转录水平呈正相关(r=0.735,P<0.05),RANTES表达水平与RANTESmRNA转录水平亦呈正相关(r=0.694,P<0.05)。结论:雌激素诱导CNP的形成可能与调节转录因子STAT-1、促进RANTES分子的表达及炎症反应有关。
