目的分析T细胞活化免疫球蛋白抑制V型结构域(V-domain Ig suppressor of T cell activation,VISTA)在幼年特发性关节炎(juvenile idiopathic arthritis,JIA)患儿外周血的表达情况,探讨其在发病中的作用。方法前瞻性收集不同亚型JIA患儿...目的分析T细胞活化免疫球蛋白抑制V型结构域(V-domain Ig suppressor of T cell activation,VISTA)在幼年特发性关节炎(juvenile idiopathic arthritis,JIA)患儿外周血的表达情况,探讨其在发病中的作用。方法前瞻性收集不同亚型JIA患儿(47例)及健康儿童(10例)的外周血,利用流式细胞术检测CD14+单核细胞、CD4+T淋巴细胞、CD8+T淋巴细胞上VISTA、干扰素-γ(interfern-γ,IFN-γ)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)的表达情况。结果VISTA在JIA患儿中的表达水平比健康儿童低(P<0.05);不同亚型JIA患儿VISTA表达差异有统计学意义,以全身型表达水平最低(P<0.05);不同免疫细胞表达VISTA差异有统计学意义,单核细胞表面VISTA表达水平更高(P<0.05)。相关性分析发现CD4+T细胞上VISTA与IFN-γ(r=-0.436,P<0.05)、TNF-α(r=-0.382,P<0.05)表达呈负相关,CD8+T细胞上VISTA与IFN-γ(r=-0.348,P<0.05)、TNF-α(r=-0.487,P<0.05)表达呈负相关;CD14+单核细胞上VISTA与IFN-γ(r=-0.582,P<0.05)、TNF-α(r=-0.603,P<0.05)表达呈负相关。结论VISTA表达不足可能与JIA发病相关,增强VISTA的免疫调节作用可能是未来治疗JIA的途径之一。展开更多
Background: Immunoglobulins have immune-modulating capacities and are used for the treatment of different dermatological diseases. They have also been reported for the treatment of severe atopic dermatitis (AD). Objec...Background: Immunoglobulins have immune-modulating capacities and are used for the treatment of different dermatological diseases. They have also been reported for the treatment of severe atopic dermatitis (AD). Objectives: To determine the effects of immunoglobulins on the phenotype and function of peripheral T and B lymphocytes from patients with AD in comparison with healthy donors (HD) as controls. Methods: We studied lymphocyte activation and T-cell cytokine production from 12 patients with AD and 10 HD by multicolour flow cytometric analysis in the presence of immunoglobulins. Results: Immunoglobulins significantly inhibited T-cell activation (CD69), by 71%(AD) and by 62%(HD). Production of interferon-γand interleukin-4 was also significantly inhibited, by 44%/24%(AD) and 38%/10%(HD), respectively. In addition, CD86 expression on B lymphocytes was downregulated by 30%in AD and by 29%in HD, whereas CD23 expression was decreased without reaching statistical significance. Conclusions: Our data demonstrate that, in vitro, immunoglobulins modulate the activation and cytokine production of peripheral blood lymphocytes from both HD and patients with AD.展开更多
文摘Background: Immunoglobulins have immune-modulating capacities and are used for the treatment of different dermatological diseases. They have also been reported for the treatment of severe atopic dermatitis (AD). Objectives: To determine the effects of immunoglobulins on the phenotype and function of peripheral T and B lymphocytes from patients with AD in comparison with healthy donors (HD) as controls. Methods: We studied lymphocyte activation and T-cell cytokine production from 12 patients with AD and 10 HD by multicolour flow cytometric analysis in the presence of immunoglobulins. Results: Immunoglobulins significantly inhibited T-cell activation (CD69), by 71%(AD) and by 62%(HD). Production of interferon-γand interleukin-4 was also significantly inhibited, by 44%/24%(AD) and 38%/10%(HD), respectively. In addition, CD86 expression on B lymphocytes was downregulated by 30%in AD and by 29%in HD, whereas CD23 expression was decreased without reaching statistical significance. Conclusions: Our data demonstrate that, in vitro, immunoglobulins modulate the activation and cytokine production of peripheral blood lymphocytes from both HD and patients with AD.
