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Promotion of the articular cartilage proteoglycan degradation by T-2 toxin and selenium protective effect 被引量:16
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作者 Si-yuan LI Jun-ling CAO +4 位作者 Zhong-li SHI Jing-hong CHEN Zeng-tie ZHANG Clare E. HUGHES Bruce CATERSON 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2008年第1期22-33,共12页
Objective: To identify the relationship between T-2 toxin and Kashin-Beck disease (KBD),the effects of T-2 toxin on aggrecan metabolism in human chondrocytes and cartilage were investigated in vitro. Methods: Chondroc... Objective: To identify the relationship between T-2 toxin and Kashin-Beck disease (KBD),the effects of T-2 toxin on aggrecan metabolism in human chondrocytes and cartilage were investigated in vitro. Methods: Chondrocytes were isolated from human articular cartilage and cultured in vitro. Hyaluronic acid (HA),soluble CD44 (sCD44),IL-1β and TNF-α levels in super-natants were measured by enzyme-linked immunosorbent assay (ELISA). CD44 content in chondrocyte membrane was deter-mined by flow cytometry (FCM). CD44,hyaluronic acid synthetase-2 (HAS-2) and aggrecanases mRNA levels in chondrocytes were determined using reverse transcription polymerase chain reaction (RT-PCR). Immunocytochemical method was used to investigate expressions of BC-13,3-B-3(-) and 2-B-6 epitopes in the cartilage reconstructed in vitro. Results: T-2 toxin inhibited CD44,HAS-2,and aggrecan mRNA expressions,but promoted aggrecanase-2 mRNA expression. Meanwhile,CD44 expression was found to be the lowest in the chondrocytes cultured with T-2 toxin and the highest in control plus selenium group. In addition,ELISA results indicated that there were higher sCD44,IL-1β and TNF-α levels in T-2 toxin group. Similarly,higher HA levels were also observed in T-2 toxin group using radioimmunoprecipitation assay (RIPA). Furthermore,using monoclonal antibodies BC-13,3-B-3 and 2-B-6,strong positive immunostaining was found in the reconstructed cartilage cultured with T-2 toxin,whereas no positive staining or very weak staining was observed in the cartilage cultured without T-2 toxin. Selenium could partly inhibit the effects of T-2 toxin above. Conclusion: T-2 toxin could inhibit aggrecan synthesis,promote aggrecanases and pro-inflammatory cytokines production,and consequently induce aggrecan degradation in chondrocytes. These will perturb metabolism balance between aggrecan synthesis and degradation in cartilage,inducing aggrecan loss in the end,which may be the initiation of the cartilage degradation. 展开更多
关键词 t-2 toxin Kashin-Beck disease (KBD) AGGRECAN IL-1β TNF-α AGGRECANASE Hyaluronic acid (HA) CD44
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Epide miological Study on the Cause of Osteoarthrosis Caused by T_2 Toxin in the Grain
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作者 孙殿军 刘云起 高本 《哈尔滨医科大学学报》 CAS 1999年第3期176-181,共6页
采用 Kallman 等人的诊断标准,对哈尔滨市区和富裕、双城、尚志等地农村50 岁以上380 例普通居民手骨关节病( Osteoarthrosis , O A) 进行 X 线检查。结果表明,各地的手 O A 检出率均超过60 % ... 采用 Kallman 等人的诊断标准,对哈尔滨市区和富裕、双城、尚志等地农村50 岁以上380 例普通居民手骨关节病( Osteoarthrosis , O A) 进行 X 线检查。结果表明,各地的手 O A 检出率均超过60 % 以上,病情不容轻视。利用病例对照调查法观察与中老年人手 O A 相关的危险因素,发现与主食种类有关,面粉( O R= 3 .02) 、玉米面( O R= 2 .41) 与大米( O R= 0 .30) 相比,是手 O A 的危险因素。采用历史前瞻性调查方法,证明了主食中 T2 毒素含量与手 O A 检出率之间存在“剂量效应”联系。据此,作者认为 T2 毒素有可能成为 O A 的致病因子之一。 展开更多
关键词 骨关节病 病因 t-2病毒 流行病学
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