Objective: To study the function of cellular immunity of patients with urticaria. Methods:T-lymphocytes subpopulations of the peripheral Mood ire 60 patients with urticaria and 40 healthy controls were examined by fla...Objective: To study the function of cellular immunity of patients with urticaria. Methods:T-lymphocytes subpopulations of the peripheral Mood ire 60 patients with urticaria and 40 healthy controls were examined by flaw cytometry. Results: The number of CD3+ and CD4+ cells in the urticaria, group were significantly lower than tliose in the control group (P<0. 01) , especially in patients with acute urticaria. Conclusion: There was immunologic dysfunction of T lymphocyte in the patients with urticaria, and not only humoral immunity takes part but also cellular immunity plays a certain role in the pathogenesis of urticaria.展开更多
Objective： To investigate whether human dendritic cells （DC） derived from peripheral blood mononuclear cells （PBMC）, which were pulsed by heat shock protein 70 （HSP70） isolated from human bladder tumor cell lin...Objective： To investigate whether human dendritic cells （DC） derived from peripheral blood mononuclear cells （PBMC）, which were pulsed by heat shock protein 70 （HSP70） isolated from human bladder tumor cell lines of E J, were able to induce peptide specific cytotoxic T-lymphocytes （CTL） response in vitro and give the experimental foundation for the future clinical trials of immunotherapy in bladder tumor. Methods： The E J-derived HSP70 co-cultured with DC from the healthy volunteers＇ PBMC, along with the crude lysate （the supematant before HSP70 purification） from EJ cells were used as the experimental groups and DC not pulsed by any tumor cells antigen were the blank control. The autologous T-lymphocytes were added into the above various DC groups, and after incubation, the stimulation indexes （SI） and interferon-y （IFN-γ） were detected to evaluate the immune activities of various DC groups. The killing effects of CTL to target cells, EJ and Hela cells, were determined with 51^Cr releasing test. Results： Both DC/HSP70 and DC/the crude lysate could effectively activate CTL in vitro and kill target cells EJ. The killing effect of DC/HSP70 to EJ was much stronger than DC/the crude lysate （the supernatant before HSP70 purification） （P 〈 0.05）. DC without any tumor cell antigens had a lower killing power to EJ. Meanwhile, DC/ HSP70 had little killing power to Hela non-relevant to bladder tumor histopathologically as compared with EJ cells （P 〈 0.05）. Conclusion： The DC pulsed by HSP70 derived from the autologous tumor cells could induce a peptide complexes specific CTL response to tumor cells, and the CTL response induced by the DC/HSP70 was stronger, which display the basis of the possible clinical application of DC/HSP70 for bladder tumor.展开更多
Experimental study was conducted to examine the effect of copper excess on the peripheral blood T-lymphocyte by the methods of flow cytometry （FCM） and experimental pathology. 420 one-day-old Avian chickens were ran...Experimental study was conducted to examine the effect of copper excess on the peripheral blood T-lymphocyte by the methods of flow cytometry （FCM） and experimental pathology. 420 one-day-old Avian chickens were randomly divided into seven groups, and fed on diets as follows： 1. controls （Cu 11mg/kg） and 2. copper excess （ Cu 100mg/kg, copper excess group Ⅰ; Cu 200mg/kg, copper excess group Ⅱ; Cu 30（hng/kg, copper excess group Ⅲ; Cu 400mg/kg, copper excess group Ⅳ; Cu 500mg/kg, copper excess group Ⅴ; Cu 600mg/kg, copper excess group VI） for six weeks. The results were as follows- 1 ） In thymus, lymphocytes in the medulla were decreased in number in copper excess groups Ⅲ,Ⅳ,Ⅴ, and Ⅵ, and the increased and enlarged thymic corpuscles and the proliferated reticular cells were also observed in both copper excess group V and copper excess group VI in comparison with those of control group. 2） The percentage of CD4＋ T cells was markedly decreased from 2 to 6 weeks of age in copper excess groups Ⅳ, Ⅴ and Ⅵ （P 〈0.05 or P 〈0.01）. 3） The percentage of CD8＋ T cell was not varied in six copper excess groups during the experiment when compared with that of control group （ P 〉 0.05 ）. 4） The CD4 ＋ / CD8＋ ratio was lower from 2 to 6 weeks of age in copper excess groups Ⅳ, Ⅴ and VI than in control group （ P 〈0.05 or P 〈0.01）. 5） It was concluded that dietary copper in excess of 300mg / kg suppressed the development of T-lymphocytes and reduced the percentage of CD4＋ T cells and the CD4＋/CD8＋ ratio, and resulted in pathological injury of the thymus. Cellular immune function was finally impaired.展开更多
Recently,fuzzy multi-sets have come to the forefront of scientists’interest and have been used in algebraic structures such asmulti-groups,multirings,anti-fuzzy multigroup and(α,γ)-anti-fuzzy subgroups.In this pape...Recently,fuzzy multi-sets have come to the forefront of scientists’interest and have been used in algebraic structures such asmulti-groups,multirings,anti-fuzzy multigroup and(α,γ)-anti-fuzzy subgroups.In this paper,we first summarize the knowledge about the algebraic structure of fuzzy multi-sets such as(α,γ)-anti-multi-fuzzy subgroups.In a way,the notion of anti-fuzzy multigroup is an application of anti-fuzzy multi sets to the theory of group.The concept of anti-fuzzy multigroup is a complement of an algebraic structure of a fuzzy multi set that generalizes both the theories of classical group and fuzzy group.The aim of this paper is to highlight the connection between fuzzy multi-sets and algebraic structures from an anti-fuzzification point of view.Therefore,in this paper,we define(α,γ)-antimulti-fuzzy subgroups,(α,γ)-anti-multi-fuzzy normal subgroups,(α,γ)-antimulti-fuzzy homomorphism on(α,γ)-anti-multi-fuzzy subgroups and these been explicated some algebraic structures.Then,we introduce the concept(α,γ)-anti-multi-fuzzy subgroups and(α,γ)-anti-multi-fuzzy normal subgroups and of their properties.This new concept of homomorphism as a bridge among set theory,fuzzy set theory,anti-fuzzy multi sets theory and group theory and also shows the effect of anti-fuzzy multi sets on a group structure.Certain results that discuss the(α,γ)cuts of anti-fuzzy multigroup are explored.展开更多
基金Supported by Grant from Janssen Science Research Foundation(JS2001008)
文摘Objective: To study the function of cellular immunity of patients with urticaria. Methods:T-lymphocytes subpopulations of the peripheral Mood ire 60 patients with urticaria and 40 healthy controls were examined by flaw cytometry. Results: The number of CD3+ and CD4+ cells in the urticaria, group were significantly lower than tliose in the control group (P<0. 01) , especially in patients with acute urticaria. Conclusion: There was immunologic dysfunction of T lymphocyte in the patients with urticaria, and not only humoral immunity takes part but also cellular immunity plays a certain role in the pathogenesis of urticaria.
基金Supported by a grant from the National Natural Science Foundation of China (No. 3000754).
文摘Objective： To investigate whether human dendritic cells （DC） derived from peripheral blood mononuclear cells （PBMC）, which were pulsed by heat shock protein 70 （HSP70） isolated from human bladder tumor cell lines of E J, were able to induce peptide specific cytotoxic T-lymphocytes （CTL） response in vitro and give the experimental foundation for the future clinical trials of immunotherapy in bladder tumor. Methods： The E J-derived HSP70 co-cultured with DC from the healthy volunteers＇ PBMC, along with the crude lysate （the supematant before HSP70 purification） from EJ cells were used as the experimental groups and DC not pulsed by any tumor cells antigen were the blank control. The autologous T-lymphocytes were added into the above various DC groups, and after incubation, the stimulation indexes （SI） and interferon-y （IFN-γ） were detected to evaluate the immune activities of various DC groups. The killing effects of CTL to target cells, EJ and Hela cells, were determined with 51^Cr releasing test. Results： Both DC/HSP70 and DC/the crude lysate could effectively activate CTL in vitro and kill target cells EJ. The killing effect of DC/HSP70 to EJ was much stronger than DC/the crude lysate （the supernatant before HSP70 purification） （P 〈 0.05）. DC without any tumor cell antigens had a lower killing power to EJ. Meanwhile, DC/ HSP70 had little killing power to Hela non-relevant to bladder tumor histopathologically as compared with EJ cells （P 〈 0.05）. Conclusion： The DC pulsed by HSP70 derived from the autologous tumor cells could induce a peptide complexes specific CTL response to tumor cells, and the CTL response induced by the DC/HSP70 was stronger, which display the basis of the possible clinical application of DC/HSP70 for bladder tumor.
基金the National Natural Science Foundation of China(No.30471304)the Sichuan Education Department(2002A002)Sichuan Scientific & Technical Department(03JY029-030-2)
文摘Experimental study was conducted to examine the effect of copper excess on the peripheral blood T-lymphocyte by the methods of flow cytometry （FCM） and experimental pathology. 420 one-day-old Avian chickens were randomly divided into seven groups, and fed on diets as follows： 1. controls （Cu 11mg/kg） and 2. copper excess （ Cu 100mg/kg, copper excess group Ⅰ; Cu 200mg/kg, copper excess group Ⅱ; Cu 30（hng/kg, copper excess group Ⅲ; Cu 400mg/kg, copper excess group Ⅳ; Cu 500mg/kg, copper excess group Ⅴ; Cu 600mg/kg, copper excess group VI） for six weeks. The results were as follows- 1 ） In thymus, lymphocytes in the medulla were decreased in number in copper excess groups Ⅲ,Ⅳ,Ⅴ, and Ⅵ, and the increased and enlarged thymic corpuscles and the proliferated reticular cells were also observed in both copper excess group V and copper excess group VI in comparison with those of control group. 2） The percentage of CD4＋ T cells was markedly decreased from 2 to 6 weeks of age in copper excess groups Ⅳ, Ⅴ and Ⅵ （P 〈0.05 or P 〈0.01）. 3） The percentage of CD8＋ T cell was not varied in six copper excess groups during the experiment when compared with that of control group （ P 〉 0.05 ）. 4） The CD4 ＋ / CD8＋ ratio was lower from 2 to 6 weeks of age in copper excess groups Ⅳ, Ⅴ and VI than in control group （ P 〈0.05 or P 〈0.01）. 5） It was concluded that dietary copper in excess of 300mg / kg suppressed the development of T-lymphocytes and reduced the percentage of CD4＋ T cells and the CD4＋/CD8＋ ratio, and resulted in pathological injury of the thymus. Cellular immune function was finally impaired.
基金Yibin University Pre-research Project,Research on the coupling and coordinated development ofmanufacturing and logistics industry under the background of intelligentmanufacturing,(2022YY001)Sichuan ProvincialDepartment of EducationWater Transport EconomicResearch Center,Research on the Development Path and Countermeasures of the Advanced Manufacturing Industry in the Sanjiang New District of Yibin under a“dual circulation”development pattern(SYJJ2020A06).
文摘Recently,fuzzy multi-sets have come to the forefront of scientists’interest and have been used in algebraic structures such asmulti-groups,multirings,anti-fuzzy multigroup and(α,γ)-anti-fuzzy subgroups.In this paper,we first summarize the knowledge about the algebraic structure of fuzzy multi-sets such as(α,γ)-anti-multi-fuzzy subgroups.In a way,the notion of anti-fuzzy multigroup is an application of anti-fuzzy multi sets to the theory of group.The concept of anti-fuzzy multigroup is a complement of an algebraic structure of a fuzzy multi set that generalizes both the theories of classical group and fuzzy group.The aim of this paper is to highlight the connection between fuzzy multi-sets and algebraic structures from an anti-fuzzification point of view.Therefore,in this paper,we define(α,γ)-antimulti-fuzzy subgroups,(α,γ)-anti-multi-fuzzy normal subgroups,(α,γ)-antimulti-fuzzy homomorphism on(α,γ)-anti-multi-fuzzy subgroups and these been explicated some algebraic structures.Then,we introduce the concept(α,γ)-anti-multi-fuzzy subgroups and(α,γ)-anti-multi-fuzzy normal subgroups and of their properties.This new concept of homomorphism as a bridge among set theory,fuzzy set theory,anti-fuzzy multi sets theory and group theory and also shows the effect of anti-fuzzy multi sets on a group structure.Certain results that discuss the(α,γ)cuts of anti-fuzzy multigroup are explored.
