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Stone Typesetting System
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《China's Foreign Trade》 1994年第1期19-19,共1页
World-known computer manufacturers are making efforts to develop high-grade typesetting systems. In 1993, the Zhuhai Stone Computer Typesetting System Development Company and Beijing Stone Modem Typesetting and Printi... World-known computer manufacturers are making efforts to develop high-grade typesetting systems. In 1993, the Zhuhai Stone Computer Typesetting System Development Company and Beijing Stone Modem Typesetting and Printing Equipment Company, both subsidiaries of the Beijing Stone Group, marketed the Stone Quick and Easy Tvpesetting Module and 展开更多
关键词 HIGH Stone typesetting System
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Parameter Design of Current Double Closed Loop for T-Type Three-Level Grid-Connected Inverter
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作者 Tiankui Sun Mingming Shi +3 位作者 Xiaolong Xiao Ping He Yu Ji Zhiyuan Yuan 《Energy Engineering》 EI 2023年第7期1621-1636,共16页
To reduce current harmonics caused by switching frequency,T-type grid-connected inverter topology with LCL filter is adopted.In view of the disadvantages of the slow response speed of the traditional current control a... To reduce current harmonics caused by switching frequency,T-type grid-connected inverter topology with LCL filter is adopted.In view of the disadvantages of the slow response speed of the traditional current control and the failure to eliminate the influence of the LCL filter on the grid-connected current by using current PI control alone,a current double closed loop PI current tracking control is proposed.Through the theoretical analysis of the grid-connected inverter control principle,the grid-connected inverter control model is designed,and the transfer functionmodel of each control link is deduced,and the current loop PI regulator is designed at last.The simulation results show that the control strategy is feasible. 展开更多
关键词 t-type inverter active damping current double closed loop
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盾构隧道通用环管片拟合排版技术
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作者 张稳军 张弛 +2 位作者 起建兵 牛荣健 张高乐 《地下空间与工程学报》 CSCD 北大核心 2024年第5期1648-1655,共8页
为实现通用环排版计算步骤和结果与管片实际偏差计算在隧道坐标下的统一,以准确、高效开展基于隧道轴线的管片轴线拟合,进而优化盾构通用环管片排版技术,首先明确了隧道三维坐标系的选取方法及盾构管片空间姿态的表示方法,提出了盾构通... 为实现通用环排版计算步骤和结果与管片实际偏差计算在隧道坐标下的统一,以准确、高效开展基于隧道轴线的管片轴线拟合,进而优化盾构通用环管片排版技术,首先明确了隧道三维坐标系的选取方法及盾构管片空间姿态的表示方法,提出了盾构通用环管片排版端面中心三维坐标的计算方法及起始环的定位方法。根据某盾构隧道工程实例,利用研发软件进行排版拟合,结果表明:该方法得到的排版结果与隧道设计轴线的拟合效果良好;将拟合得到的管片排版端面中心坐标与实际测量结果进行对比,能够得到施工偏差方向及具体数值,可为后续排版和纠偏提供定量控制指导,该研究成果还可实现管片楔形量的试算,从而优化管片设计参数,为盾构隧道的设计及建设提供技术支持。 展开更多
关键词 盾构隧道 通用环管片 排版技术
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Design of a distributed power amplifier based on T-type matching networks 被引量:1
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作者 张瑛 马凯学 +1 位作者 周洪敏 郭宇锋 《Journal of Southeast University(English Edition)》 EI CAS 2016年第3期278-284,共7页
The impedance characteristics of distributed amplifiers are analyzed based on T-type matching networks, and a distributed power amplifier consisting of three gain cells is proposed. Non-uniform T-type matching network... The impedance characteristics of distributed amplifiers are analyzed based on T-type matching networks, and a distributed power amplifier consisting of three gain cells is proposed. Non-uniform T-type matching networks are adopted to make the impedance of artificial transmission lines connected to the gate and drain change stage by stage gradually, which provides good impedance matching and improves the output power and efficiency. The measurement results show that the amplifier gives an average forward gain of 6 dB from 3 to 16. 5 GHz. In the desired band, the input return loss is typically less than - 9. 5 dB, and the output return loss is better than -8.5 dB. The output power at 1-dB gain compression point is from 3.6 to 10. 6 dBm in the band of 2 to 16 GHz while the power added efficiency (PAE) is from 2% to 12. 5% . The power consumption of the amplifier is 81 mW with a supply of 1.8 V, and the chip area is 0.91 mm × 0.45 mm. 展开更多
关键词 distributed amplifier impedance matching poweradded efficiency t-type network
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钢结构箱形旋转楼梯制作技术
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作者 吴鹏 王磊 +1 位作者 徐玲珑 荀天懿 《工程建设与设计》 2024年第5期204-206,共3页
在钢结构箱形旋转楼梯的生产制作过程中,先对图纸进行分析,建立BIM三维实体模型,在实体模型上找出尺寸控制点,绘制胎具放样图,搭设旋转楼梯专用组焊胎具,同时使用基于PKPM系统下的学思软件进行排版,通过对旋转楼梯生产过程中的下料尺寸... 在钢结构箱形旋转楼梯的生产制作过程中,先对图纸进行分析,建立BIM三维实体模型,在实体模型上找出尺寸控制点,绘制胎具放样图,搭设旋转楼梯专用组焊胎具,同时使用基于PKPM系统下的学思软件进行排版,通过对旋转楼梯生产过程中的下料尺寸、坡口角度、装配精度和焊接参数等对产品质量有着关键影响的因素进行严格控制,有效保证了钢结构箱形旋转楼梯的制作质量。 展开更多
关键词 钢结构 箱形 旋转楼梯 放样 排版 装配 焊接
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略谈语言文字学文献的整理
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作者 张可 《现代语文》 2024年第6期4-10,F0002,共8页
语言文字学文献不仅在中华文明历史传承过程中起到了关键作用,对于今天也同样具有重要的价值和意义。对语言文字学文献进行系统整理,有利于学术研究者和广大读者亲近经典、阅读经典、传承经典,推动中华优秀传统文化创造性转化、创新性... 语言文字学文献不仅在中华文明历史传承过程中起到了关键作用,对于今天也同样具有重要的价值和意义。对语言文字学文献进行系统整理,有利于学术研究者和广大读者亲近经典、阅读经典、传承经典,推动中华优秀传统文化创造性转化、创新性发展。同时,严谨、规范的整理成果也为未来的数字化出版做好了准备,有利于充分发挥其作为工具书的效用。语言文字学文献的整理,必须重视古籍整理的规范,尊重文献本身的特性,切合目标读者的需求,在整理形式、版本选择、录排、标点、校勘及辅文撰写等多方面加以注意。 展开更多
关键词 语言文字学 文献整理 录排 标点 校勘 辅文
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字体在排版设计中的侵权问题与规避策略
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作者 杜玉娇 《中国科技纵横》 2024年第13期129-131,156,共4页
文字承载着多元化元素,其作为感官载体能够向观看者呈现明确含义,是期刊排版中不可或缺的组成元素。此外,文字可塑性较强,是一种视觉艺术的表现形式,能够展现出千变万化的风貌和深邃丰富的情感,让画面充满设计感和丰富色彩。近年来,随... 文字承载着多元化元素,其作为感官载体能够向观看者呈现明确含义,是期刊排版中不可或缺的组成元素。此外,文字可塑性较强,是一种视觉艺术的表现形式,能够展现出千变万化的风貌和深邃丰富的情感,让画面充满设计感和丰富色彩。近年来,随着公民普法意识的不断提高,对知识产权权益的保护也随之加强。而字体版权问题往往最容易在期刊排版过程中被忽视。为了降低设计字体的侵权风险,本文阐述了字体版权侵权的界定,分析了导致字体侵权的原因,提出了相应规避策略,以期提高期刊排版过程中的字体排版应用水平。 展开更多
关键词 排版 设计 字体侵权
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服装定制新量具——中分双向(及可折叠式)人体量体软尺
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作者 田顺发 田源 《服装设计师》 2024年第5期93-97,共5页
本文介绍了服装定制领域量体新量具,即中分双向人体量体软尺,也着重介绍了该量具的具体使用方法。它可以精准测量出服装制板时所需用到的人体各部位的数据,让普通量体制板师也可以用客户人体数据制板,极大地弥补了定制行业在过去所存在... 本文介绍了服装定制领域量体新量具,即中分双向人体量体软尺,也着重介绍了该量具的具体使用方法。它可以精准测量出服装制板时所需用到的人体各部位的数据,让普通量体制板师也可以用客户人体数据制板,极大地弥补了定制行业在过去所存在的诸多缺陷,使服装定制能够真正做到一人一板。 展开更多
关键词 服装 私人定制 双向尺 量体制版
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方正书版和方正飞翔在机关文印排版设计中的应用与质量提升策略
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作者 宋慧芳 《网印工业》 2024年第8期68-70,共3页
本文旨在深入探讨机关文印排版设计的现状、挑战及解决策略,重点介绍各种设计工具的应用,分析核心设计要素,并提出提升设计质量的方法。通过技术应用与创新实践,期望推动机关文印排版设计的现代化发展。
关键词 方正书版 方正飞翔 机关文印 排版设计 技术应用 创新实践
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Calcium signaling and T-type calcium channels in cancer cell cycling 被引量:13
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作者 James T Taylor Xiang-Bin Zeng +6 位作者 Jonathan E Pottle Kevin Lee Alun R Wang Stephenie G Yi lennifer A S Scruggs Suresh S Sikka Ming Li 《World Journal of Gastroenterology》 SCIE CAS CSCD 2008年第32期4984-4991,共8页
Regulation of intracellular calcium is an important signaling mechanism for cell proliferation in both normal and cancerous cells. In normal epithelial cells, free calcium concentration is essential for cells to enter... Regulation of intracellular calcium is an important signaling mechanism for cell proliferation in both normal and cancerous cells. In normal epithelial cells, free calcium concentration is essential for cells to enter and accomplish the S phase and the M phase of the cell cycle. In contrast, cancerous cells can pass these phases of the cell cycle with much lower cytoplasmic free calcium concentrations, indicating an alternative mechanism has developed for fulfilling the intracellular calcium requirement for an increased rate of DNA synthesis and mitosis of fast replicating cancerous cells. The detailed mechanism underlying the altered calcium loading pathway remains unclear; however, there is a growing body of evidence that suggests the T-type Ca2+ channel is abnormally expressed in cancerous cells and that blockade of these channels may reduce cell proliferation in addition to inducing apoptosis. Recent studies also show that the expression of T-type Ca2+ channels in breast cancer cells is proliferation state dependent, i.e. the channels are expressed at higher levels during the fast-replication period, and once the cells are in a non-proliferation state, expression of this channel isminimal. Therefore, selectively blocking calcium entry into cancerous cells may be a valuable approach for preventing tumor growth. Since T-type Ca2+ channels are not expressed in epithelial cells, selective T-type Ca2+ channel blockers may be useful in the treatment of certain types of cancers. 展开更多
关键词 t-type calcium channels CANCER CELLCYCLE CALCIUM
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Laparoscopic pancreatic duct incision and stone removal and T-type tube drainage for pancreatic duct stone: A case report and review of literature 被引量:8
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作者 Yang Bai Shi-An Yu +1 位作者 Li-Yuan Wang Dao-Jun Gong 《World Journal of Clinical Cases》 SCIE 2018年第13期679-682,共4页
To study a more micro-invasive procedure for patients having pancreatic duct stones(PDS).Till now,there has been no report of laparoscopic pancreatic duct incision and stone removal and T-type tube drainage for PDS in... To study a more micro-invasive procedure for patients having pancreatic duct stones(PDS).Till now,there has been no report of laparoscopic pancreatic duct incision and stone removal and T-type tube drainage for PDS in the English literature.An 82-year-old man suffered from subxiphoid pain associated with a dilated pancreatic duct(7 mm)containing one stone,but without a mass in the head of the pancreas.Laparoscopic pancreatic duct incision,stone removal,and T-type tube drainage were successfully performed without intraoperative or postoperative complications.An uneventful operation was performed with laparoscopically completed procedures in 160 min.The intraoperative loss of blood was around 50 mL.After patient a discharge on day 11,complete relief from the subxiphoid pain was reported at a follow-up visit 15 mo later.Laparoscopic pancreatic duct incision with stone removal and T-type tube drainage is applicable in carefully selected patients and can be effectively and safely used for the treatment of the abdominal pain of chronic pancreatitis. 展开更多
关键词 LAPAROSCOPY Pancreatic duct STONE t-type TUBE drainage Chronic PANCREATITIS Surgery
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Resistance Characteristics of Hydraulic Oil through Isodiametric T-type Duct with Sharp Corners 被引量:6
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作者 TANG Jinglin WANG Liwei LI Xia 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2009年第2期250-255,共6页
Rational determination and reduction of local energy loss of oil flow at pipe junctions are of important significance to improve hydraulic pipeline's work efficiency, especially for complex hydraulic pipeline connect... Rational determination and reduction of local energy loss of oil flow at pipe junctions are of important significance to improve hydraulic pipeline's work efficiency, especially for complex hydraulic pipeline connected by isodiametric T-type ducts with sharp comers to get combined and divided flow. From this point of view, the formulae of resistance loss for combined flow and divided flow through isodiametric T-type duct with sharp comers as well as the correlations of resistance loss coefficients in the branches of the duct are derived using energy method. On this basis, resistance characteristics of hydraulic oil in the duct are obtained by numerical simulation of different flow modes, which are commonly applied in hydraulic pipelines, using computational fluid dynamics (CFD) method, and the reasons for the resistance loss are analyzed based on the pressure change mechanism in the flow field. A part of simulation results was validated with the reference data. The research shows that for combined flows the resistance loss of symmetrical is lower than that of unsymmetrical to obtain low speed in common branch, but to gain high speed is quite the contrary, for divided flows, the symmetrical is always a reasonable choice to reduce resistance loss. These conclusions can be applied to optimize the design of hydraulic pipeline. 展开更多
关键词 hydraulic oil t-type duct energy method computational fluid dynamics (CFD) resistance characteristic
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Locking compression plate+T-type steel plate for postoperative weight bearing and functional recovery in complex tibial plateau fractures 被引量:6
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作者 Hai-Feng Li Tao Yu +2 位作者 Xing-Fei Zhu Hua Wang Ying-Qi Zhang 《World Journal of Clinical Cases》 SCIE 2022年第2期502-510,共9页
Complex tibial plateau fractures can seriously affect quality of life and physical and mental health of patients.The anatomical relationship between the proximal tibial bone and soft tissue is complex,resulting in dif... Complex tibial plateau fractures can seriously affect quality of life and physical and mental health of patients.The anatomical relationship between the proximal tibial bone and soft tissue is complex,resulting in different types of tibial plateau fractures.Violent trauma can lead to displaced fracture,serious soft tissue injury,and potentially,dislocation of the knee joint.Therefore,tibial plateau fractures are extremely unstable.AIM To assess the use of locking compression plate(LCP)+T-type steel plate for postoperative weight bearing and functional recovery of complex tibial plateau fractures.METHODS Ninety-seven patients with complex tibial plateau fractures who underwent surgery at our hospital were selected for retrospective study.Forty-nine patients had been treated with LCP+T-type steel plate limited internal fixation(study group),and 48 patients with bilateral ordinary steel plate support(control group).The operation process index,postoperative rehabilitation related index,Rasmussen score of the knee joint,tibial plateau varus angle(TPA),tibial plateau retroversion angle(PA),and surgical complications of the two groups were compared.RESULTS The operation time and intraoperative bone graft volume in the study group were lower than those in the control group(P<0.05).There were no significant differences in surgical bleeding,anterior external incision length,postoperative drainage,hospital stay duration,and fracture healing time between the groups(P>0.05).There was no significant difference in the TPA and PA angle between the groups immediately and 18 mo after surgery(P>0.05).At 12 mo after surgery,the Rasmussen scale score was higher in the study group than in the control group(P<0.05).There was no significant difference in the Rasmussen scale score at 18 mo after surgery,and the radiology score at 12 and 18 mo after surgery,between the two groups(P>0.05).The postoperative complication rate in the study group(3.77%)was lower than that in the control group(15.09%;P<0.05).CONCLUSION LCP+T-type steel plate internal fixation has advantages in terms of minimizing trauma and enabling early postoperative functional exercise,promoting functional recovery and lower limb weight-bearing,and reducing postoperative complications. 展开更多
关键词 Locking compression plate t-type steel plate COMPLEXITY Tibial plateau fracture Functional recovery COMPLICATIONS
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Evaluation of hollow fiber T-type zeolite membrane modules for ethanol dehydration 被引量:7
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作者 Xuerui Wang Ji Jiang +3 位作者 Dezhong Liu Youquan Xue Chun Zhang Xuehong Gu 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2017年第5期581-586,共6页
This work presents the design of hollow fiber T-type zeolite membrane modules with different geometric configurations. The module performances were evaluated by pervaporation dehydration of ethanol/water mixtures. Str... This work presents the design of hollow fiber T-type zeolite membrane modules with different geometric configurations. The module performances were evaluated by pervaporation dehydration of ethanol/water mixtures. Strong concentration polarization was found for the modules with big membrane bundles. The concentration polarization was enhanced at high temperature due to the higher water permeation flux. The increase of feed flow could improve water permeation flux for the membrane modules with small membrane bundle.Computational fluid dynamics was used to visualize the flow field distribution inside of the modules with different configurations. The membrane module with seven bundles exhibited highest separation efficiency due to the uniform distribution of flow rate. The packing density could be 10 times higher than that of the tubular membrane module. The hollow fiber membrane module exhibited good stability for ethanol dehydration. 展开更多
关键词 Hollow fiber Module Pervaporation t-type zeolite membrane
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Inhibitory actions of mibefradil on steroidogenesis in mouse Leydig cells: involvement of Ca^2+ entry via the T-type Ca^2+ channel 被引量:1
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作者 Jae-Ho Lee Jong-Uk Kim +1 位作者 Changhoon Kim Churl K. Min 《Asian Journal of Andrology》 SCIE CAS CSCD 2010年第6期807-813,共7页
Intracellular cAMP and Ca^2+ are involved in the regulation of steroidogenic activity in Leydig cells, which coordinate responses to luteinizing hormone (LH) and human ehorionic gonadotropin (hCG). However, the i... Intracellular cAMP and Ca^2+ are involved in the regulation of steroidogenic activity in Leydig cells, which coordinate responses to luteinizing hormone (LH) and human ehorionic gonadotropin (hCG). However, the identification of Ca^2+ entry implicated in Leydig cell steroidogenesis is not well defined. The objective of this study was to identify the type of Ca^2+ channel that affects Leydig cell steroidogenesis. In vitro steroidogenesis in the freshly dissociated Leydig cells of mice was induced by hCG incubation. The effects of mibefradil (a putative T-type Ca^2+ channel blocker) on steroidogenesis were assessed using reverse transcription (RT)-polymerase chain reaction analysis for the steroidogenic acute regulatory protein (STAR) mRNA expression and testosterone production using radioimmunoassay. In the presence of 1.0 mmol L-1 extracellular Ca^2+, hCG at 1 to 100 IU noticeably elevated both StAR mRNA level and testosterone secretion (P 〈 0.05), and the stimulatory effects of hCG were markedly diminished by mibefradil in a dose-dependent manner (P 〈 0.05). Moreover; the hCG-induced increase in testosterone production was completely removed when external Ca^2+ was omitted, implying that Ca entry is needed for hCG-induced steroidogenesis. Furthermore, a patch-clamp study revealed the presence of mibefradil-sensitive Ca^24- currents seen at a concentration range that nearly paralleled those inhibiting steroidogenesis. Collectively, Our data provide evidence that hCG-stimulated steroidogenesis is mediated at least in part by Ca^2+ entry carried out by the T-type Ca^2+ channel in the Leydig cells of mice. 展开更多
关键词 Leydig cells MIBEFRADIL STAR steroidogenesis t-type Ca^2+ channel
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Ultra wideband CMOS digital T-type attenuator with low phase errors 被引量:1
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作者 Chao Fan Yahua Ran Liqun Ye 《Journal of Semiconductors》 EI CAS CSCD 2022年第3期57-59,共3页
A proposed inductive-phase-compensation ultra wideband CMOS digital T-type attenuator design based on an analysis of minimising phase errors is presented in this letter.In a standard CMOS technology,the proposed atten... A proposed inductive-phase-compensation ultra wideband CMOS digital T-type attenuator design based on an analysis of minimising phase errors is presented in this letter.In a standard CMOS technology,the proposed attenuator is analytically demonstrated to have low phase errors due to the inductive-phase-compensation network.A design equation is inferred and a wide-band 4dB attenuation bit digital attenuator with low phase errors is designed as a test vehicle for the proposed approach. 展开更多
关键词 ULTRA-WIDEBAND digital t-type attenuator low phase error inductive-phase-compensation CMOS
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Actuating mechanism and design of a T-type linear ultrasonic motor
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作者 刘英想 刘军考 +1 位作者 陈维山 石胜君 《Journal of Harbin Institute of Technology(New Series)》 EI CAS 2011年第2期43-46,共4页
To take advantage of merits of higher output-power and efficiency of bolt-clamped transducer,a T-type linear ultrasonic motor was proposed in this paper.The proposed motor contained a horizontal transducer and a verti... To take advantage of merits of higher output-power and efficiency of bolt-clamped transducer,a T-type linear ultrasonic motor was proposed in this paper.The proposed motor contained a horizontal transducer and a vertical transducer.The horizontal transducer included two exponential shape horns,and the vertical transducer contained one exponential shape horn.And the horns intersected at tip ends where a driving foot was located.The longitudinal vibrations of transducers were excited by the stretching vibration of PZT ceramics.Longitudinal vibrations were superimposed in the motor and generated elliptical trajectory at the driving foot.The actuating mechanism of the proposed motor was analyzed.The two working modals of the motor were gained with FEM.The resonant frequencies of two working modals were degenerated by adjusting the structural parameters.The motion trajectory of nodes on the driving foot was gained by transient analysis.The vibration amplitude of node on the driving foot indicates that the proposed motor has potential good output characteristics.The results verify the feasibility of the proposed design and provide instructions for the development of the new ultrasonic motor. 展开更多
关键词 ultrasonic motor LINEAR t-type longitudinal transducer FEM
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Design and Implementation of a Three-Phase Active T-Type NPC Inverter for Low-Voltage Microgrids
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作者 Rick Chen Ying-Yu Tzou 《Energy and Power Engineering》 2017年第4期70-77,共8页
This paper presents the design and implementation of a 3 kVA three-phase active T-type neutral-point clamped (NPC) inverter with GaN power devices for low-voltage microgrids. The designed inverter is used in a battery... This paper presents the design and implementation of a 3 kVA three-phase active T-type neutral-point clamped (NPC) inverter with GaN power devices for low-voltage microgrids. The designed inverter is used in a battery-based energy system (BESS) for power conversion optimization in applications to low-voltage microgrids. A modular design method has been developed for the design and implementation of the AT-NPC inverter. Experimental verification has been carried out based on a 3-kW three-phase T-Type NPC grid-connected inverter. FPGA based digital control technique has been developed for the current control of the three-level three-phase grid inverter. A maximum efficiency of 98.49% has been achieved within a load range from 50% to 75%. 展开更多
关键词 ACTIVE t-type NPC Inverter Modular Design Methodology Loss Analysis Efficiency Optimization
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T-type calcium channel expression in cultured human neuroblastoma cells
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作者 Xianjie Wen Shiyuan Xu +4 位作者 Lingling Wang Hua Liang Chengxiang Yang Hanbing Wang Hongzhen Liu 《Neural Regeneration Research》 SCIE CAS CSCD 2011年第31期2410-2413,共4页
Human neuroblastoma cells (SH-SY5Y) have similar structures and functions as neural cells and have been frequently used for cell culture studies of neural cell functions.Previous studies have revealed L-and N-type c... Human neuroblastoma cells (SH-SY5Y) have similar structures and functions as neural cells and have been frequently used for cell culture studies of neural cell functions.Previous studies have revealed L-and N-type calcium channels in SH-SY5Y cells.However,the distribution of the low-voltage activated calcium channel (namely called T-type calcium channel,including Cav3.1,Cav3.2,and Cav3.3) in SH-SY5Y cells remains poorly understood.The present study detected mRNA and protein expres-sion of the T-type calcium channel (Cav3.1,Cav3.2,and Cav3.3) in cultured SH-SY5Y cells using real-time polymerase chain reaction (PCR) and western blot analysis.Results revealed mRNA and protein expression from all three T-type calcium channel subtypes in SH-SY5Y cells.Moreover,Cav3.1 was the predominant T-type calcium channel subtype in SH-SY5Y cells. 展开更多
关键词 calcium ion channel calcium signaling cell culture neural regeneration real-time PCR t-type calcium channel western blot neural regeneration
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Macrophage migration inhibitory factor decreased T-type Ca^(2+) channel current through activating Src
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作者 RAO Fang,DENG Chun-yu,WU Shu-lin,YU Xi-yong,XIAO Ding-zhang,HUANG Wei,KUANG Su-juan,LIN Qiu-xiong, ShanZhi-xin (Department of Cardiology,Guangdong Cardiovascular Institute, Guangzhou 510100,China) 《岭南心血管病杂志》 2011年第S1期196-196,共1页
Aims T-type Ca<sup>2+</sup> current(I<sub>CaT</sub>)plays an important role in the pathogenesis of atrial fibrillation(AF).The present study sought to investigate the role of Macrophage migra... Aims T-type Ca<sup>2+</sup> current(I<sub>CaT</sub>)plays an important role in the pathogenesis of atrial fibrillation(AF).The present study sought to investigate the role of Macrophage migration inhibitory factor(MIF),a pleiotropic cytokine,in the regulation of T-type Ca<sup>2+</sup> channel in atrium myocytes.Methods We used whole-cell voltage-clamp technique and biochemical assays to study the regulation and expression of I<sub>Ca</sub>,T in mouse atrium myocytes(HL-1 cells).Results Serum MIF concentrations was slightly increased in patients with AF compared to sinus rhythm(SR) controls.In cultured HL-1 cells, significant amounts of MIF were produced in response to hydrogen peroxide(H<sub>2</sub>O<sub>2</sub>),but not AngiotensinⅡ(AngⅡ). Mouse recombinant MIF(rMIF)(20 or 40 nM,24 h) suppressed peak ICa,T by-38%and-60%in a concentration-dependent manner,impaired the voltage-dependent activation of I<sub>Ca</sub>,T,and down-regulated of TCC alG mRNA.Src inhibitors genistein and PPl significantly enhanced ICaT.The depression of ICa,T induced by rMIF could be reversed by genistein and PP1.Conclusions MIFis involved in the pathogenesis of AF,probably by decreasing ICa,T through impairment of the channel function and activation of c-Src kinases in atrium myocytes. 展开更多
关键词 type channel current through activating Src MIF Macrophage migration inhibitory factor decreased t-type Ca
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